aspsirna3180 details

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ASPsiRNA information siRNA Id:aspsirna3180
siRNA Name: siSN
Mismatch information Wild siRNA (As strand 3'->5'):CUUAACUUAGUGGGCUUGU
ASP-siRNA (As strand 3'->5'):CUUAACUUAGUGGGCUUGU
Mismatch position in siRNA: NA
Gene Information Gene Name Cyclin-dependent kinase 2 (CDK2)
Target Sequence (5'->3'):NA
Wild allele (5'->3'):GAAUUGAAUCACCCGAACA
Mutant allele (5'->3'):GAAUUGAAUCACCCGAACA
Respective Gene/Protein Resources GenBank Accession:NA
HUGO ID:1771
Reference SNp(RefSNP): NA
Disease/Mutation information Target Mutation: NA
Disease:NA
KEGG disease ID:NA
OMIM ID: NA
COSMIC: CDK2
DECIPHER: CDK2
GeneTests: CDK2
ASP siRNA details Mutant allele (5'->3'): GAAUUGAAUCACCCGAACA
ASP-siRNA (As strand 3'->5'):CUUAACUUAGUGGGCUUGU
Percentage efficacy of ASP-siRNA for mutant allele:90
Wild allele (5'->3'): GAAUUGAAUCACCCGAACA
ASP-siRNA (As strand 3'->5'):CUUAACUUAGUGGGCUUGU
Percentage efficacy of ASP-siRNA for wild allele:55
Relative difference:35
Wild siRNA details Wild allele (5'->3'): GAAUUGAAUCACCCGAACA
Wild siRNA (As strand 3'->5'):CUUAACUUAGUGGGCUUGU
Percentage efficacy of wild siRNA for wild allele:80
Wild allele (5'->3'): GAAUUGAAUCACCCGAACA
ASP-siRNA (As strand 3'->5'):CUUAACUUAGUGGGCUUGU
Percentage efficacy of Wild sirna for mutant allele:100
Relative difference :-20
General information Mismatch incorporated in siRNA/Target:siRNA
Cell-line used: hTERT-RPE1
Experimental technique used: Fluorescence microscopy
Transfection method: RNAiMAX
siRNA expression method: Life Technologies
Post-transfection duration: 48 hours
Concentration used:25 nM
Reference:25586224
Delivery method:Adeno-Associated Virus (rAAV) mediated
Article title:Efficient CRISPR-rAAV engineering of endogenous genes to study protein function by allele-specific RNAi
Authors:Kaulich M, Lee YJ, Lönn P, Springer AD, Meade BR, Dowdy SF
Journal Reference:Nucleic Acids Res. 2015 Apr 20;43(7):e45. doi: 10.1093/nar/gku1403. Epub 2015 Jan 13