| | |
|---|
| ASPsiRNA information |
siRNA Id: | aspsirna3180
|
|
siRNA Name: | siSN
|
| Mismatch information |
Wild siRNA (As strand 3'->5'): | CUUAACUUAGUGGGCUUGU
|
|
ASP-siRNA (As strand 3'->5'): | CUUAACUUAGUGGGCUUGU
|
|
Mismatch position in siRNA: | NA |
| Gene Information |
Gene Name | Cyclin-dependent kinase 2 (CDK2) |
|
Target Sequence (5'->3'): | NA
|
|
Wild allele (5'->3'): | GAAUUGAAUCACCCGAACA
|
|
Mutant allele (5'->3'): | GAAUUGAAUCACCCGAACA
|
| Respective Gene/Protein Resources |
GenBank Accession: | NA |
| HUGO ID: | 1771 |
|
Reference SNp(RefSNP): | NA |
| Disease/Mutation information |
Target Mutation: | NA
|
|
Disease: | NA |
|
KEGG disease ID: | NA |
|
OMIM ID: | NA |
|
COSMIC: | CDK2 |
|
DECIPHER: | CDK2 |
|
GeneTests: | CDK2 |
| ASP siRNA details |
Mutant allele (5'->3'): | GAAUUGAAUCACCCGAACA
|
|
ASP-siRNA (As strand 3'->5'): | CUUAACUUAGUGGGCUUGU
|
|
Percentage efficacy of ASP-siRNA for mutant allele: | 90
|
|
Wild allele (5'->3'): | GAAUUGAAUCACCCGAACA
|
|
ASP-siRNA (As strand 3'->5'): | CUUAACUUAGUGGGCUUGU
|
|
Percentage efficacy of ASP-siRNA for wild allele: | 55
|
|
Relative difference: | 35
|
| Wild siRNA details |
Wild allele (5'->3'): | GAAUUGAAUCACCCGAACA
|
|
Wild siRNA (As strand 3'->5'): | CUUAACUUAGUGGGCUUGU
|
|
Percentage efficacy of wild siRNA for wild allele: | 80
|
|
Wild allele (5'->3'): | GAAUUGAAUCACCCGAACA
|
|
ASP-siRNA (As strand 3'->5'): | CUUAACUUAGUGGGCUUGU
|
|
Percentage efficacy of Wild sirna for mutant allele: | 100
|
|
Relative difference : | -20
|
| General information |
Mismatch incorporated in siRNA/Target: | siRNA |
| Cell-line used: | hTERT-RPE1 |
| Experimental technique used: | Fluorescence microscopy |
| Transfection method: | RNAiMAX |
| siRNA expression method: | Life Technologies |
| Post-transfection duration: | 48 hours |
| Concentration used: | 25 nM |
| Reference: | 25586224 |
| Delivery method: | Adeno-Associated Virus (rAAV) mediated |
'| Article title: | Efficient CRISPR-rAAV engineering of endogenous genes to study protein function by allele-specific RNAi |
'| Authors: | Kaulich M, Lee YJ, Lönn P, Springer AD, Meade BR, Dowdy SF |
'| Journal Reference: | Nucleic Acids Res. 2015 Apr 20;43(7):e45. doi: 10.1093/nar/gku1403. Epub 2015 Jan 13 |
'
