aspsirna3258 details

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ASPsiRNA information siRNA Id:aspsirna3258
siRNA Name: lv-shRNA167delinsWL-8
Mismatch information Wild siRNA (As strand 3'->5'):CCGGAGAAUGAACCUA
ASP-siRNA (As strand 3'->5'):CCGGAGAACCGAGAACCUA
Mismatch position in siRNA: delAinsGGCT
Gene Information Gene Name keratin 9 (KRT9)
Target Sequence (5'->3'):CTCGGCTGGCCTCTTACTTGGATAAGGTGCAGGCT
Wild allele (5'->3'):GGCCUCUUACUUGGAU
Mutant allele (5'->3'):GGCCUCUUGGCUCUUGGAU
Position of siRNA on target gene: 558-573
Respective Gene/Protein Resources GenBank Accession:NM_000226.3
Cytogenic location:17q21.2
Chromosomal coordinates:17:39,722,092-39,728,309
UniProt ID:P35527
HUGO ID:6447
Reference SNp(RefSNP): rs59317143
Disease/Mutation information Target Mutation: c.500delAinsGGCT,p.Tyr167delinsTrpLeu
Matation type/variant: InDel/inframe_variant
Mutation at gene level: NG_008300.1:g.5566delAinsGGCT
Pathogenic status of mutation: Pathogenic
Disease:Epidermolytic palmoplantar keratoderma (EPPK)
Clinical Resources ClinVar ID:66157
KEGG disease ID:H00722
OMIM ID: 144200
COSMIC: KRT9
DECIPHER: KRT9
GeneTests: KRT9
ASP siRNA details Mutant allele (5'->3'): GGCCUCUUGGCUCUUGGAU
ASP-siRNA (As strand 3'->5'):CCGGAGAACCGAGAACCUA
Percentage efficacy of ASP-siRNA for mutant allele:80
Wild allele (5'->3'): GGCCUCUUACUUGGAU
ASP-siRNA (As strand 3'->5'):CCGGAGAACCGAGAACCUA
Percentage efficacy of ASP-siRNA for wild allele:2
Relative difference:78
Wild siRNA details Wild allele (5'->3'): GGCCUCUUACUUGGAU
Wild siRNA (As strand 3'->5'):CCGGAGAAUGAACCUA
Percentage efficacy of wild siRNA for wild allele:40
Wild allele (5'->3'): GGCCUCUUACUUGGAU
ASP-siRNA (As strand 3'->5'):CCGGAGAACCGAGAACCUA
Percentage efficacy of Wild sirna for mutant allele:30
Relative difference :10
General information Mismatch incorporated in siRNA/Target:siRNA
Cell-line used: Transgenic mouse model
Experimental technique used: Quantitative immunoblotting
Transfection method: Lipofectamine 2000
siRNA expression method: Genechem
Post-transfection duration: 10 days
Concentration used:0 - 6.25 nM
Reference:27003758
Delivery method:Lentivirus-mediated shRNA delivery/Injection
Article title:A Small Indel Mutant Mouse Model of Epidermolytic Palmoplantar Keratoderma and Its Application to Mutant-specific shRNA Therapy
Authors:Ya-Su Lyu, Pei-liang Shi, Xiao-Ling Chen, Yue-Xiao Tang, Yan-Fang Wang, Rong-Rong Liu, Xiao-Rui Luan, Yu Fang4, Ru-Huan Mei4 Zhen-Fang Du, Hai-Ping Ke5, Erik Matro6, Ling-En Li, Zhao-Yu Lin, Jing Zhao, Xiang Gao and Xian-Ning Zhang
Journal Reference:Mol Ther Nucleic Acids. 2016 Mar 22;5:e299. doi: 10.1038/mtna.2016.17