aspsirna3435 details

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ASPsiRNA information siRNA Id:aspsirna3435
siRNA Name: sh39Ap10
Mismatch information Wild siRNA (As strand 3'->5'):CCCUGUCAUGAAGUUGCGAA
ASP-siRNA (As strand 3'->5'):CCCUGUCAUUAAGUUGCGAA
Mismatch position in siRNA: U10G
Gene Information Gene Name Hungtintin (HTT)
Target Sequence (5'->3'):AGCTTTGATGGATTCTAA
Wild allele (5'->3'):GGGACAGUACUUCAACGCUU
Mutant allele (5'->3'):GGGACAGUAAUUCAACGCUU
Position of siRNA on target gene: 5472-5489
Respective Gene/Protein Resources GenBank Accession:NM_002111.7
Cytogenic location:4p16.3
Chromosomal coordinates:4:3,076,407-3,245,686
UniProt ID:P42858
HUGO ID:4851
Reference SNp(RefSNP): rs363125
Disease/Mutation information Target Mutation: NM_002111.7:c.5165C>A
Matation type/variant: Single nucleotide variant/Missense variant
Mutation at gene level: NG_009378.1:g.118146C>A
Pathogenic status of mutation: Pathogenic
Disease:Huntington disease (HD)
Clinical Resources ClinVar ID:NA
KEGG disease ID:H00059
OMIM ID: 143100
COSMIC: HTT
DECIPHER: HTT
GeneTests: HTT
ASP siRNA details Mutant allele (5'->3'): GGGACAGUAAUUCAACGCUU
ASP-siRNA (As strand 3'->5'):CCCUGUCAUUAAGUUGCGAA
Percentage efficacy of ASP-siRNA for mutant allele:85
Wild allele (5'->3'): GGGACAGUACUUCAACGCUU
ASP-siRNA (As strand 3'->5'):CCCUGUCAUUAAGUUGCGAA
Percentage efficacy of ASP-siRNA for wild allele:10
Relative difference:75
Wild siRNA details Wild allele (5'->3'): GGGACAGUACUUCAACGCUU
Wild siRNA (As strand 3'->5'):CCCUGUCAUGAAGUUGCGAA
Percentage efficacy of wild siRNA for wild allele:80
Wild allele (5'->3'): GGGACAGUACUUCAACGCUU
ASP-siRNA (As strand 3'->5'):CCCUGUCAUUAAGUUGCGAA
Percentage efficacy of Wild sirna for mutant allele:5
Relative difference :75
General information Mismatch incorporated in siRNA/Target:siRNA
Cell-line used: HEK293T
Experimental technique used: RT-PCR
Transfection method: Lipofectamine 2000
siRNA expression method: Invitrogen
Post-transfection duration: 48 hours
Concentration used:6nM
Reference:24926995
Delivery method:Lentiviral vector mediated shRNA delivery
Article title:Allele-specific silencing of mutant huntingtin in rodent brain and human stem cells.
Authors:Drouet V, Ruiz M, Zala D, Feyeux M, Auregan G, Cambon K, Troquier L, Carpentier J, Aubert S, Merienne N, Bourgois-Rocha F, Hassig R, Rey M, Dufour N, Saudou F, Perrier AL, Hantraye P, Déglon N.
Journal Reference:PLoS One. 2014 Jun 13;9(6):e99341. doi: 10.1371/journal.pone.0099341. eCollection 2014