aspsirna3540 details

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ASPsiRNA information siRNA Id:aspsirna3540
siRNA Name: siC10
Mismatch information Wild siRNA (As strand 3'->5'):GUCGUCGUCCCCCUGGAUAG
ASP-siRNA (As strand 3'->5'):GUCGUCGUCGCCCUGGAUAG
Mismatch position in siRNA: G11C
Gene Information Gene Name Ataxin-3 (ATXN3)
Target Sequence (5'->3'):CAGCAGCAGCAGGGGGACCTATCAGGAC
Wild allele (5'->3'):CAGCAGCAGGGGGACCUAUC
Mutant allele (5'->3'):CAGCAGCAGCGGGACCUAUC
Position of siRNA on target gene: 976-995
Respective Gene/Protein Resources GenBank Accession:NM_004993.5
Cytogenic location:14q32.12
Chromosomal coordinates:14:92,524,895-92,572,964
UniProt ID:P54252
HUGO ID:7106
Reference SNp(RefSNP): rs19392292
Disease/Mutation information Target Mutation: c.G987C,c.892_894CAG(8_36) (p.Gln298_Gln305=)
Matation type/variant: Microsatellite
Mutation at gene level: NG_008198.2:g.40588_40590CAG(8_36)
Pathogenic status of mutation: Disease causing trinucleotide CAG repeats
Disease:Machado-Joseph Disease (MJD)/Spinocerebellar ataxia type 3 (SCA3)
Clinical Resources ClinVar ID:3551
KEGG disease ID:H00063
OMIM ID: 109150
COSMIC: ATXN3
DECIPHER: ATXN3
GeneTests: ATXN3
ASP siRNA details Mutant allele (5'->3'): CAGCAGCAGCGGGACCUAUC
ASP-siRNA (As strand 3'->5'):GUCGUCGUCGCCCUGGAUAG
Percentage efficacy of ASP-siRNA for mutant allele:66
Wild allele (5'->3'): CAGCAGCAGGGGGACCUAUC
ASP-siRNA (As strand 3'->5'):GUCGUCGUCGCCCUGGAUAG
Percentage efficacy of ASP-siRNA for wild allele:62
Relative difference:4
Wild siRNA details Wild allele (5'->3'): CAGCAGCAGGGGGACCUAUC
Wild siRNA (As strand 3'->5'):GUCGUCGUCCCCCUGGAUAG
Percentage efficacy of wild siRNA for wild allele:49
Wild allele (5'->3'): CAGCAGCAGGGGGACCUAUC
ASP-siRNA (As strand 3'->5'):GUCGUCGUCGCCCUGGAUAG
Percentage efficacy of Wild sirna for mutant allele:20
Relative difference :29
General information Mismatch incorporated in siRNA/Target:siRNA
Cell-line used: Cos-7
Experimental technique used: Western blot
Transfection method: Lipofectamine Plus
siRNA expression method: Integrated DNA Technologies
Post-transfection duration: 24-48 hours
Concentration used:5mg
Reference:US20080176812A1
Delivery method:Adenovirus associated shRNA delivery
Article title:Allele-specific silencing of disease genes.
Authors:Davidson Beverly L, Harper Scott, Gonzalez-Alegre Pedro,Miller Victor, Paulson Henry
Journal Reference:US2008/0176812 A1