aspsirna3550 details

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ASPsiRNA information siRNA Id:aspsirna3550
siRNA Name: siA9/C8
Mismatch information Wild siRNA (As strand 3'->5'):CACCGGUCCACCUUCAUUUUAG
ASP-siRNA (As strand 3'->5'):CACCGGUGUACCUUCAUUUUAG
Mismatch position in siRNA: U14C,G15C
Gene Information Gene Name Microtubule-associated protein tau (MAPT)
Target Sequence (5'->3'):GUGGCCAGGUGGAAGUAAAAUC
Wild allele (5'->3'):GUGGCCAGGUGGAAGUAAAAUC
Mutant allele (5'->3'):GUGGCCACAUGGAAGUAAAAUC
Position of siRNA on target gene: 2274-2295
Respective Gene/Protein Resources GenBank Accession:NM_016835.4
Cytogenic location:17q21.31
Chromosomal coordinates:17:43,971,701-44,105,699
UniProt ID:P10636
HUGO ID:6893
Reference SNp(RefSNP): rs63750570
Disease/Mutation information Target Mutation: c.1960G>A,p.Val654Met
Matation type/variant: Single nucleotide variant/Missense variant
Mutation at gene level: NG_007398.1:g.129219G>A
Pathogenic status of mutation: Disease causing
Disease:Frontotemporal dementia and parkinsonism linked to chromosome 17 (FTDP-17)
Clinical Resources ClinVar ID:14252
KEGG disease ID:H00078
OMIM ID: 600274
COSMIC: MAPT
DECIPHER: MAPT
GeneTests: MAPT
ASP siRNA details Mutant allele (5'->3'): GUGGCCACAUGGAAGUAAAAUC
ASP-siRNA (As strand 3'->5'):CACCGGUGUACCUUCAUUUUAG
Percentage efficacy of ASP-siRNA for mutant allele:65
Wild allele (5'->3'): GUGGCCAGGUGGAAGUAAAAUC
ASP-siRNA (As strand 3'->5'):CACCGGUGUACCUUCAUUUUAG
Percentage efficacy of ASP-siRNA for wild allele:0
Relative difference:65
Wild siRNA details Wild allele (5'->3'): GUGGCCAGGUGGAAGUAAAAUC
Wild siRNA (As strand 3'->5'):CACCGGUCCACCUUCAUUUUAG
Percentage efficacy of wild siRNA for wild allele:49
Wild allele (5'->3'): GUGGCCAGGUGGAAGUAAAAUC
ASP-siRNA (As strand 3'->5'):CACCGGUGUACCUUCAUUUUAG
Percentage efficacy of Wild sirna for mutant allele:20
Relative difference :29
General information Mismatch incorporated in siRNA/Target:siRNA
Cell-line used: Cos-7
Experimental technique used: Western blot
Transfection method: Lipofectamine Plus
siRNA expression method: Integrated DNA Technologies
Post-transfection duration: 24 hours
Concentration used:2.5mg
Reference:US20080176812A1
Delivery method:Adenovirus associated shRNA delivery
Article title:Allele-specific silencing of disease genes.
Authors:Davidson Beverly L, Harper Scott, Gonzalez-Alegre Pedro,Miller Victor, Paulson Henry
Journal Reference:US2008/0176812 A1