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| ASPsiRNA information |
siRNA Id: | aspsirna3556
|
|
siRNA Name: | siA9/C8
|
| Mismatch information |
Wild siRNA (As strand 3'->5'): | CACCGGUCCACCUUCAUUUUAG
|
|
ASP-siRNA (As strand 3'->5'): | CACCGGUGUACCUUCAUUUUAG
|
|
Mismatch position in siRNA: | U14C,G15C |
| Gene Information |
Gene Name | Torsin family 1 member A (TOR1A) |
|
Target Sequence (5'->3'): | GUGGCCAGGUGGAAGUAAAAUC
|
|
Wild allele (5'->3'): | GUGGCCAGGUGGAAGUAAAAUC
|
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Mutant allele (5'->3'): | GUGGCCACAUGGAAGUAAAAUC
|
|
Position of siRNA on target gene: | NA
|
| Respective Gene/Protein Resources |
GenBank Accession: | NM_000113 |
| Cytogenic location: | NA |
| Chromosomal coordinates: | NA |
| UniProt ID: | O14656 |
| HUGO ID: | 3098 |
|
Reference SNp(RefSNP): | NA |
| Disease/Mutation information |
Target Mutation: | NA
|
|
Matation type/variant: | Single nucleotide variant/Missense variant
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Mutation at gene level: | NA
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Pathogenic status of mutation: | Disease causing
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|
Disease: | Frontotemporal dementia and parkinsonism linked to chromosome 17 (FTDP-17) |
| Clinical Resources |
ClinVar ID: | NA |
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KEGG disease ID: | H00078 |
|
OMIM ID: | 600274 |
|
COSMIC: | TOR1A |
|
DECIPHER: | TOR1A |
|
GeneTests: | TOR1A |
| ASP siRNA details |
Mutant allele (5'->3'): | GUGGCCACAUGGAAGUAAAAUC
|
|
ASP-siRNA (As strand 3'->5'): | CACCGGUGUACCUUCAUUUUAG
|
|
Percentage efficacy of ASP-siRNA for mutant allele: | 55
|
|
Wild allele (5'->3'): | GUGGCCAGGUGGAAGUAAAAUC
|
|
ASP-siRNA (As strand 3'->5'): | CACCGGUGUACCUUCAUUUUAG
|
|
Percentage efficacy of ASP-siRNA for wild allele: | 0
|
|
Relative difference: | 55
|
| Wild siRNA details |
Wild allele (5'->3'): | GUGGCCAGGUGGAAGUAAAAUC
|
|
Wild siRNA (As strand 3'->5'): | CACCGGUCCACCUUCAUUUUAG
|
|
Percentage efficacy of wild siRNA for wild allele: | 49
|
|
Wild allele (5'->3'): | GUGGCCAGGUGGAAGUAAAAUC
|
|
ASP-siRNA (As strand 3'->5'): | CACCGGUGUACCUUCAUUUUAG
|
|
Percentage efficacy of Wild sirna for mutant allele: | 20
|
|
Relative difference : | 29
|
| General information |
Mismatch incorporated in siRNA/Target: | siRNA
|
| Cell-line used: | HeLa |
| Experimental technique used: | Western blot |
| Transfection method: | Lipofectamine Plus |
| siRNA expression method: | Integrated DNA Technologies |
| Post-transfection duration: | 24 hours |
| Concentration used: | 2.5mg |
| Reference: | US20080176812A1 |
| Delivery method: | Adenovirus associated shRNA delivery |
| Article title: | Allele-specific silencing of disease genes. |
| Authors: | Davidson Beverly L, Harper Scott, Gonzalez-Alegre Pedro,Miller Victor, Paulson Henry |
| Journal Reference: | US2008/0176812 A1 |
