| | |
ASPsiRNA information |
siRNA Id: | aspsirna3766
|
siRNA Name: | 190
|
Mismatch information |
Wild siRNA (As strand 3'->5'): | NA
|
ASP-siRNA (As strand 3'->5'): | UUUCAACGCGCCUCCUCAA
|
Mismatch position in siRNA: | NA |
Gene Information |
Gene Name | Peptidylprolyl isomerase B (PPIB)/Cyclophilin B |
Target Sequence (5'->3'): | CGCAGGCAAAGACACCAACGGCTCCCAGTTCTTCAT
|
Wild allele (5'->3'): | NA
|
Mutant allele (5'->3'): | AAAGUUGCGCGGAGGAGUU
|
Position of siRNA on target gene: | 595-630(t)
|
Respective Gene/Protein Resources |
GenBank Accession: | NM_000942.4 |
Cytogenic location: | 15q22.31 |
Chromosomal coordinates: | 15:64,448,013-64,455,353 |
UniProt ID: | P23284 |
HUGO ID: | 9255 |
Reference SNp(RefSNP): | NA |
Disease/Mutation information |
Target Mutation: | NA
|
Matation type/variant: | Single nucleotide variant/Missense variant
|
Mutation at gene level: | NG_012979.1:g.12045_12048delACAG
|
Pathogenic status of mutation: | Pathogenic
|
Disease: | PPIB-Related Osteogenesis Imperfecta (Type IX) |
Clinical Resources |
ClinVar ID: | NA |
KEGG disease ID: | H00506 |
OMIM ID: | 166200 |
COSMIC: | PPIB/Cyclophilin B |
DECIPHER: | PPIB/Cyclophilin B |
GeneTests: | PPIB/Cyclophilin B |
ASP siRNA details |
Mutant allele (5'->3'): | AAAGUUGCGCGGAGGAGUU
|
ASP-siRNA (As strand 3'->5'): | UUUCAACGCGCCUCCUCAA
|
Percentage efficacy of ASP-siRNA for mutant allele: | 90
|
Wild allele (5'->3'): | NA
|
ASP-siRNA (As strand 3'->5'): | UUUCAACGCGCCUCCUCAA
|
Percentage efficacy of ASP-siRNA for wild allele: | NA
|
Relative difference: | NA
|
Wild siRNA details |
Wild allele (5'->3'): | NA
|
Wild siRNA (As strand 3'->5'): | NA
|
Percentage efficacy of wild siRNA for wild allele: | NA
|
Wild allele (5'->3'): | NA
|
ASP-siRNA (As strand 3'->5'): | UUUCAACGCGCCUCCUCAA
|
Percentage efficacy of Wild sirna for mutant allele: | NA
|
Relative difference : | NA
|
General information |
Mismatch incorporated in siRNA/Target: | siRNA |
Cell-line used: | HeLa |
Experimental technique used: | Dual luciferase reporter assay |
Transfection method: | Lipofectamine 2000 |
siRNA expression method: | 2' ACE-chemistry |
Post-transfection duration: | 24 hours |
Concentration used: | 100nM |
Reference: | 16489337 |
Delivery method: | Transfection |
'Article title: | 3' UTR seed matches, but not overall identity, are associated with RNAi off-targets. |
'Authors: | Birmingham A, Anderson EM, Reynolds A, Ilsley-Tyree D, Leake D, Fedorov Y, Baskerville S, Maksimova E, Robinson K, Karpilow J, Marshall WS, Khvorova A. |
'Journal Reference: | Nat Methods. 2006 Mar;3(3):199-204 |
'