aspsirna3767 details

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ASPsiRNA information siRNA Id:aspsirna3767
siRNA Name: 191
Mismatch information Wild siRNA (As strand 3'->5'):UUUCAACGCGCCUCCUCAA
ASP-siRNA (As strand 3'->5'):UUUCAACGCACCUCCUCAA
Mismatch position in siRNA: A10G
Gene Information Gene Name Peptidylprolyl isomerase B (PPIB)/Cyclophilin B
Target Sequence (5'->3'):CGCAGGCAAAGACACCAACGGCTCCCAGTTCTTCAT
Wild allele (5'->3'):AAAGUUGCGCGGAGGAGUU
Mutant allele (5'->3'):AAAGUUGCGCGGAGGAGUU
Position of siRNA on target gene: 595-630(t)
Respective Gene/Protein Resources GenBank Accession:NM_000942.4
Cytogenic location:15q22.31
Chromosomal coordinates:15:64,448,013-64,455,353
UniProt ID:P23284
HUGO ID:9255
Reference SNp(RefSNP): NA
Disease/Mutation information Target Mutation: NA
Matation type/variant: Single nucleotide variant/Missense variant
Mutation at gene level: NG_012979.1:g.12045_12048delACAG
Pathogenic status of mutation: Pathogenic
Disease:PPIB-Related Osteogenesis Imperfecta (Type IX)
Clinical Resources ClinVar ID:NA
KEGG disease ID:H00506
OMIM ID: 166200
COSMIC: PPIB/Cyclophilin B
DECIPHER: PPIB/Cyclophilin B
GeneTests: PPIB/Cyclophilin B
ASP siRNA details Mutant allele (5'->3'): AAAGUUGCGCGGAGGAGUU
ASP-siRNA (As strand 3'->5'):UUUCAACGCACCUCCUCAA
Percentage efficacy of ASP-siRNA for mutant allele:90
Wild allele (5'->3'): AAAGUUGCGCGGAGGAGUU
ASP-siRNA (As strand 3'->5'):UUUCAACGCACCUCCUCAA
Percentage efficacy of ASP-siRNA for wild allele:70
Relative difference:20
Wild siRNA details Wild allele (5'->3'): AAAGUUGCGCGGAGGAGUU
Wild siRNA (As strand 3'->5'):UUUCAACGCGCCUCCUCAA
Percentage efficacy of wild siRNA for wild allele:90
Wild allele (5'->3'): AAAGUUGCGCGGAGGAGUU
ASP-siRNA (As strand 3'->5'):UUUCAACGCACCUCCUCAA
Percentage efficacy of Wild sirna for mutant allele:70
Relative difference :20
General information Mismatch incorporated in siRNA/Target:siRNA
Cell-line used: HeLa
Experimental technique used: Dual luciferase reporter assay
Transfection method: Lipofectamine 2000
siRNA expression method: 2' ACE-chemistry
Post-transfection duration: 24 hours
Concentration used:100nM
Reference:16489337
Delivery method:Transfection
Article title:3' UTR seed matches, but not overall identity, are associated with RNAi off-targets.
Authors:Birmingham A, Anderson EM, Reynolds A, Ilsley-Tyree D, Leake D, Fedorov Y, Baskerville S, Maksimova E, Robinson K, Karpilow J, Marshall WS, Khvorova A.
Journal Reference:Nat Methods. 2006 Mar;3(3):199-204