aspsirna3887 details

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ASPsiRNA information siRNA Id:aspsirna3887
siRNA Name: siRNA-4_12U
Mismatch information Wild siRNA (As strand 3'->5'):CCAAUUGUCGCUAGACUAC
ASP-siRNA (As strand 3'->5'):CCAAUUGUCGCUAGACUAC
Mismatch position in siRNA: U:G
Gene Information Gene Name Transforming growth factor, beta receptor II (TGFBR2)
Target Sequence (5'->3'):CCGCACGTTCCCAAGTCGGTTAACAGCGATCTGATG
Wild allele (5'->3'):GGUUAACAGCGAUCUGAUG
Mutant allele (5'->3'):GGUUAACGGCGAUCUGAUG
Position of siRNA on target gene: 344-362
Respective Gene/Protein Resources GenBank Accession:NM_031132.3
Cytogenic location:3p24.1
Chromosomal coordinates:3:30,647,993-30,735,633
UniProt ID:P37173
HUGO ID:NA
Reference SNp(RefSNP): NA
Disease/Mutation information Target Mutation: NA
Matation type/variant: NA
Mutation at gene level: NA
Pathogenic status of mutation: NA
Disease:NA
Clinical Resources ClinVar ID:NA
KEGG disease ID:NA
OMIM ID: NA
COSMIC: TGFBR2
DECIPHER: TGFBR2
GeneTests: TGFBR2
ASP siRNA details Mutant allele (5'->3'): GGUUAACGGCGAUCUGAUG
ASP-siRNA (As strand 3'->5'):CCAAUUGUCGCUAGACUAC
Percentage efficacy of ASP-siRNA for mutant allele:64
Wild allele (5'->3'): GGUUAACAGCGAUCUGAUG
ASP-siRNA (As strand 3'->5'):CCAAUUGUCGCUAGACUAC
Percentage efficacy of ASP-siRNA for wild allele:95
Relative difference:31
Wild siRNA details Wild allele (5'->3'): GGUUAACAGCGAUCUGAUG
Wild siRNA (As strand 3'->5'):CCAAUUGUCGCUAGACUAC
Percentage efficacy of wild siRNA for wild allele:95
Wild allele (5'->3'): GGUUAACAGCGAUCUGAUG
ASP-siRNA (As strand 3'->5'):CCAAUUGUCGCUAGACUAC
Percentage efficacy of Wild sirna for mutant allele:45
Relative difference :50
General information Mismatch incorporated in siRNA/Target:siRNA
Cell-line used: HEK293
Experimental technique used: RT-PCR, Flourescence microscopy
Transfection method: TriZol reagent
siRNA expression method: Genepharma, Proligo Sigma
Post-transfection duration: 24 hours
Concentration used:13nM
Reference:19815667
Delivery method:Transfection
Article title:Profiling of mismatch discrimination in RNAi enabled rational design of allele-specific siRNAs.
Authors:Huang H, Qiao R, Zhao D, Zhang T, Li Y, Yi F, Lai F, Hong J, Ding X, Yang Z, Zhang L, Du Q, Liang Z.
Journal Reference:Nucleic Acids Res. 2009 Dec;37(22):7560-9. doi: 10.1093/nar/gkp835. Epub .