aspsirna3924 details

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ASPsiRNA information siRNA Id:aspsirna3924
siRNA Name: siRNA-13_12U
Mismatch information Wild siRNA (As strand 3'->5'):CAUUGACUUCCGAGCGAGU
ASP-siRNA (As strand 3'->5'):CAUUGACUUCCGAGCGAGU
Mismatch position in siRNA: U:C
Gene Information Gene Name Sphingosine 1-phosphate receptor1(S1PR1)
Target Sequence (5'->3'):CTGTAACTGAAGGCTCGCTCAACCTCGCCCT
Wild allele (5'->3'):GUAACUGAAGGCUCGCUCA
Mutant allele (5'->3'):GUAACUGCAGGCUCGCUCA
Position of siRNA on target gene: 314-332
Respective Gene/Protein Resources GenBank Accession:NM_017301.2
Cytogenic location:6q23.2
Chromosomal coordinates:6:132,269,315-132,272,517
UniProt ID:P21453
HUGO ID:NA
Reference SNp(RefSNP): NA
Disease/Mutation information Target Mutation: NA
Matation type/variant: NA
Mutation at gene level: NA
Pathogenic status of mutation: NA
Disease:NA
Clinical Resources ClinVar ID:NA
KEGG disease ID:NA
OMIM ID: NA
COSMIC: CTGF
DECIPHER: CTGF
GeneTests: CTGF
ASP siRNA details Mutant allele (5'->3'): GUAACUGCAGGCUCGCUCA
ASP-siRNA (As strand 3'->5'):CAUUGACUUCCGAGCGAGU
Percentage efficacy of ASP-siRNA for mutant allele:45
Wild allele (5'->3'): GUAACUGAAGGCUCGCUCA
ASP-siRNA (As strand 3'->5'):CAUUGACUUCCGAGCGAGU
Percentage efficacy of ASP-siRNA for wild allele:95
Relative difference:50
Wild siRNA details Wild allele (5'->3'): GUAACUGAAGGCUCGCUCA
Wild siRNA (As strand 3'->5'):CAUUGACUUCCGAGCGAGU
Percentage efficacy of wild siRNA for wild allele:94
Wild allele (5'->3'): GUAACUGAAGGCUCGCUCA
ASP-siRNA (As strand 3'->5'):CAUUGACUUCCGAGCGAGU
Percentage efficacy of Wild sirna for mutant allele:86
Relative difference :8
General information Mismatch incorporated in siRNA/Target:siRNA
Cell-line used: HEK293
Experimental technique used: RT-PCR, Flourescence microscopy
Transfection method: TriZol reagent
siRNA expression method: Genepharma, Proligo Sigma
Post-transfection duration: 24 hours
Concentration used:13nM
Reference:19815667
Delivery method:Transfection
Article title:Profiling of mismatch discrimination in RNAi enabled rational design of allele-specific siRNAs.
Authors:Huang H, Qiao R, Zhao D, Zhang T, Li Y, Yi F, Lai F, Hong J, Ding X, Yang Z, Zhang L, Du Q, Liang Z.
Journal Reference:Nucleic Acids Res. 2009 Dec;37(22):7560-9. doi: 10.1093/nar/gkp835. Epub .