aspsirna3972 details

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ASPsiRNA information siRNA Id:aspsirna3972
siRNA Name: siRNA-25_12C
Mismatch information Wild siRNA (As strand 3'->5'):CUUGAAACGACGAGUCAUA
ASP-siRNA (As strand 3'->5'):CUUGAAACGACGAGUCAUA
Mismatch position in siRNA: C:U
Gene Information Gene Name Collagen type I alpha 1 (COL1A2)
Target Sequence (5'->3'):CTGGTCTTACTGGGAACTTTGCTGCTCAGTATTC
Wild allele (5'->3'):GAACUUUGCUGCUCAGUAU
Mutant allele (5'->3'):GAACUUUUCUGCUCAGUAU
Position of siRNA on target gene: 243-261
Respective Gene/Protein Resources GenBank Accession:NM_053356.1
Cytogenic location:17q21.3
Chromosomal coordinates:17:48,261,456-48,279,002
UniProt ID:P02452
HUGO ID:NA
Reference SNp(RefSNP): NA
Disease/Mutation information Target Mutation: NA
Matation type/variant: NA
Mutation at gene level: NA
Pathogenic status of mutation: NA
Disease:NA
Clinical Resources ClinVar ID:NA
KEGG disease ID:NA
OMIM ID: NA
COSMIC: COL1A1
DECIPHER: COL1A1
GeneTests: COL1A1
ASP siRNA details Mutant allele (5'->3'): GAACUUUUCUGCUCAGUAU
ASP-siRNA (As strand 3'->5'):CUUGAAACGACGAGUCAUA
Percentage efficacy of ASP-siRNA for mutant allele:90
Wild allele (5'->3'): GAACUUUGCUGCUCAGUAU
ASP-siRNA (As strand 3'->5'):CUUGAAACGACGAGUCAUA
Percentage efficacy of ASP-siRNA for wild allele:95
Relative difference:5
Wild siRNA details Wild allele (5'->3'): GAACUUUGCUGCUCAGUAU
Wild siRNA (As strand 3'->5'):CUUGAAACGACGAGUCAUA
Percentage efficacy of wild siRNA for wild allele:99
Wild allele (5'->3'): GAACUUUGCUGCUCAGUAU
ASP-siRNA (As strand 3'->5'):CUUGAAACGACGAGUCAUA
Percentage efficacy of Wild sirna for mutant allele:70
Relative difference :29
General information Mismatch incorporated in siRNA/Target:siRNA
Cell-line used: HEK293
Experimental technique used: RT-PCR, Flourescence microscopy
Transfection method: TriZol reagent
siRNA expression method: Genepharma, Proligo Sigma
Post-transfection duration: 24 hours
Concentration used:13nM
Reference:19815667
Delivery method:Transfection
Article title:Profiling of mismatch discrimination in RNAi enabled rational design of allele-specific siRNAs.
Authors:Huang H, Qiao R, Zhao D, Zhang T, Li Y, Yi F, Lai F, Hong J, Ding X, Yang Z, Zhang L, Du Q, Liang Z.
Journal Reference:Nucleic Acids Res. 2009 Dec;37(22):7560-9. doi: 10.1093/nar/gkp835. Epub .