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| ASPsiRNA information |
siRNA Id: | aspsirna3985
|
|
siRNA Name: | siRNA-26_12C
|
| Mismatch information |
Wild siRNA (As strand 3'->5'): | CGUUUACCGGAGUGACCAC
|
|
ASP-siRNA (As strand 3'->5'): | CGUUUACCGGAGUGACCAC
|
|
Mismatch position in siRNA: | C:A |
| Gene Information |
Gene Name | Collagen type I alpha 1 (COL1A2) |
|
Target Sequence (5'->3'): | CAAATGGCCTCACTGGTGCCAAGGGAGC
|
|
Wild allele (5'->3'): | GCAAAUGGCCUCACUGGUG
|
|
Mutant allele (5'->3'): | GCAAAUGACCUCACUGGUG
|
|
Position of siRNA on target gene: | 922-940
|
| Respective Gene/Protein Resources |
GenBank Accession: | NM_053356.1 |
| Cytogenic location: | 17q21.3 |
| Chromosomal coordinates: | 17:48,261,456-48,279,002 |
| UniProt ID: | P02452 |
| HUGO ID: | NA |
|
Reference SNp(RefSNP): | NA |
| Disease/Mutation information |
Target Mutation: | NA
|
|
Matation type/variant: | NA
|
|
Mutation at gene level: | NA
|
|
Pathogenic status of mutation: | NA
|
|
Disease: | NA |
| Clinical Resources |
ClinVar ID: | NA |
|
KEGG disease ID: | NA |
|
OMIM ID: | NA |
|
COSMIC: | COL1A1 |
|
DECIPHER: | COL1A1 |
|
GeneTests: | COL1A1 |
| ASP siRNA details |
Mutant allele (5'->3'): | GCAAAUGACCUCACUGGUG
|
|
ASP-siRNA (As strand 3'->5'): | CGUUUACCGGAGUGACCAC
|
|
Percentage efficacy of ASP-siRNA for mutant allele: | 85
|
|
Wild allele (5'->3'): | GCAAAUGGCCUCACUGGUG
|
|
ASP-siRNA (As strand 3'->5'): | CGUUUACCGGAGUGACCAC
|
|
Percentage efficacy of ASP-siRNA for wild allele: | 95
|
|
Relative difference: | 10
|
| Wild siRNA details |
Wild allele (5'->3'): | GCAAAUGGCCUCACUGGUG
|
|
Wild siRNA (As strand 3'->5'): | CGUUUACCGGAGUGACCAC
|
|
Percentage efficacy of wild siRNA for wild allele: | 90
|
|
Wild allele (5'->3'): | GCAAAUGGCCUCACUGGUG
|
|
ASP-siRNA (As strand 3'->5'): | CGUUUACCGGAGUGACCAC
|
|
Percentage efficacy of Wild sirna for mutant allele: | 79
|
|
Relative difference : | 11
|
| General information |
Mismatch incorporated in siRNA/Target: | siRNA |
| Cell-line used: | HEK293 |
| Experimental technique used: | RT-PCR, Flourescence microscopy |
| Transfection method: | TriZol reagent |
| siRNA expression method: | Genepharma, Proligo Sigma |
| Post-transfection duration: | 24 hours |
| Concentration used: | 13nM |
| Reference: | 19815667 |
| Delivery method: | Transfection |
'| Article title: | Profiling of mismatch discrimination in RNAi enabled rational design of allele-specific siRNAs. |
'| Authors: | Huang H, Qiao R, Zhao D, Zhang T, Li Y, Yi F, Lai F, Hong J, Ding X, Yang Z, Zhang L, Du Q, Liang Z. |
'| Journal Reference: | Nucleic Acids Res. 2009 Dec;37(22):7560-9. doi: 10.1093/nar/gkp835. Epub . |
'
