| | |
|---|
| ASPsiRNA information |
siRNA Id: | aspsirna4035
|
|
siRNA Name: | siRNA-38_4C
|
| Mismatch information |
Wild siRNA (As strand 3'->5'): | CUCAGCUCAGCCUUUCGAG
|
|
ASP-siRNA (As strand 3'->5'): | CUCAGCUCAGCCUUUCGAG
|
|
Mismatch position in siRNA: | C:C |
| Gene Information |
Gene Name | Platelet-derived growth factor beta polypeptide (PDGFB) |
|
Target Sequence (5'->3'): | CCGAGCACATTCTGGAGTCGAGTCGGAAAGCTCATC
|
|
Wild allele (5'->3'): | GAGUCGAGUCGGAAAGCUC
|
|
Mutant allele (5'->3'): | GAGUCGAGUCGGAAACCUC
|
|
Position of siRNA on target gene: | 604-622
|
| Respective Gene/Protein Resources |
GenBank Accession: | NM_031524.1 |
| Cytogenic location: | 22q13.1 |
| Chromosomal coordinates: | 22:39,619,684-39,640,956 |
| UniProt ID: | P09619 |
| HUGO ID: | NA |
|
Reference SNp(RefSNP): | NA |
| Disease/Mutation information |
Target Mutation: | NA
|
|
Matation type/variant: | NA
|
|
Mutation at gene level: | NA
|
|
Pathogenic status of mutation: | NA
|
|
Disease: | NA |
| Clinical Resources |
ClinVar ID: | NA |
|
KEGG disease ID: | NA |
|
OMIM ID: | NA |
|
COSMIC: | PDGFB |
|
DECIPHER: | PDGFB |
|
GeneTests: | PDGFB |
| ASP siRNA details |
Mutant allele (5'->3'): | GAGUCGAGUCGGAAACCUC
|
|
ASP-siRNA (As strand 3'->5'): | CUCAGCUCAGCCUUUCGAG
|
|
Percentage efficacy of ASP-siRNA for mutant allele: | 57
|
|
Wild allele (5'->3'): | GAGUCGAGUCGGAAAGCUC
|
|
ASP-siRNA (As strand 3'->5'): | CUCAGCUCAGCCUUUCGAG
|
|
Percentage efficacy of ASP-siRNA for wild allele: | 94
|
|
Relative difference: | 37
|
| Wild siRNA details |
Wild allele (5'->3'): | GAGUCGAGUCGGAAAGCUC
|
|
Wild siRNA (As strand 3'->5'): | CUCAGCUCAGCCUUUCGAG
|
|
Percentage efficacy of wild siRNA for wild allele: | 90
|
|
Wild allele (5'->3'): | GAGUCGAGUCGGAAAGCUC
|
|
ASP-siRNA (As strand 3'->5'): | CUCAGCUCAGCCUUUCGAG
|
|
Percentage efficacy of Wild sirna for mutant allele: | 50
|
|
Relative difference : | 40
|
| General information |
Mismatch incorporated in siRNA/Target: | siRNA |
| Cell-line used: | HEK293 |
| Experimental technique used: | RT-PCR, Flourescence microscopy |
| Transfection method: | TriZol reagent |
| siRNA expression method: | Genepharma, Proligo Sigma |
| Post-transfection duration: | 24 hours |
| Concentration used: | 13nM |
| Reference: | 19815667 |
| Delivery method: | Transfection |
'| Article title: | Profiling of mismatch discrimination in RNAi enabled rational design of allele-specific siRNAs. |
'| Authors: | Huang H, Qiao R, Zhao D, Zhang T, Li Y, Yi F, Lai F, Hong J, Ding X, Yang Z, Zhang L, Du Q, Liang Z. |
'| Journal Reference: | Nucleic Acids Res. 2009 Dec;37(22):7560-9. doi: 10.1093/nar/gkp835. Epub . |
'
