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| ASPsiRNA information |
siRNA Id: | aspsirna4041
|
|
siRNA Name: | siRNA-40_17U
|
| Mismatch information |
Wild siRNA (As strand 3'->5'): | CCUUAGCGUCCGUAGUACC
|
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ASP-siRNA (As strand 3'->5'): | CCUUAGCGUCCGUAGUACC
|
|
Mismatch position in siRNA: | U:U |
| Gene Information |
Gene Name | solute carrier family 12 (potassium/chloride transporter), member 5 (SLC12A5) |
|
Target Sequence (5'->3'): | ATCGCAGGCATCATGGAGTCCTTCTGC
|
|
Wild allele (5'->3'): | GGAAUCGCAGGCAUCAUGG
|
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Mutant allele (5'->3'): | GGUAUCGCAGGCAUCAUGG
|
|
Position of siRNA on target gene: | 488-506
|
| Respective Gene/Protein Resources |
GenBank Accession: | NM_134363.1 |
| Cytogenic location: | 20q13.12 |
| Chromosomal coordinates: | 20:44,650,328-44,688,788 |
| UniProt ID: | Q9H2X9 |
| HUGO ID: | NA |
|
Reference SNp(RefSNP): | NA |
| Disease/Mutation information |
Target Mutation: | NA
|
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Matation type/variant: | NA
|
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Mutation at gene level: | NA
|
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Pathogenic status of mutation: | NA
|
|
Disease: | NA |
| Clinical Resources |
ClinVar ID: | NA |
|
KEGG disease ID: | NA |
|
OMIM ID: | NA |
|
COSMIC: | SLC12A5 |
|
DECIPHER: | SLC12A5 |
|
GeneTests: | SLC12A5 |
| ASP siRNA details |
Mutant allele (5'->3'): | GGUAUCGCAGGCAUCAUGG
|
|
ASP-siRNA (As strand 3'->5'): | CCUUAGCGUCCGUAGUACC
|
|
Percentage efficacy of ASP-siRNA for mutant allele: | 66
|
|
Wild allele (5'->3'): | GGAAUCGCAGGCAUCAUGG
|
|
ASP-siRNA (As strand 3'->5'): | CCUUAGCGUCCGUAGUACC
|
|
Percentage efficacy of ASP-siRNA for wild allele: | 84
|
|
Relative difference: | 18
|
| Wild siRNA details |
Wild allele (5'->3'): | GGAAUCGCAGGCAUCAUGG
|
|
Wild siRNA (As strand 3'->5'): | CCUUAGCGUCCGUAGUACC
|
|
Percentage efficacy of wild siRNA for wild allele: | 89
|
|
Wild allele (5'->3'): | GGAAUCGCAGGCAUCAUGG
|
|
ASP-siRNA (As strand 3'->5'): | CCUUAGCGUCCGUAGUACC
|
|
Percentage efficacy of Wild sirna for mutant allele: | 79
|
|
Relative difference : | 10
|
| General information |
Mismatch incorporated in siRNA/Target: | siRNA |
| Cell-line used: | HEK293 |
| Experimental technique used: | RT-PCR, Flourescence microscopy |
| Transfection method: | TriZol reagent |
| siRNA expression method: | Genepharma, Proligo Sigma |
| Post-transfection duration: | 24 hours |
| Concentration used: | 13nM |
| Reference: | 19815667 |
| Delivery method: | Transfection |
'| Article title: | Profiling of mismatch discrimination in RNAi enabled rational design of allele-specific siRNAs. |
'| Authors: | Huang H, Qiao R, Zhao D, Zhang T, Li Y, Yi F, Lai F, Hong J, Ding X, Yang Z, Zhang L, Du Q, Liang Z. |
'| Journal Reference: | Nucleic Acids Res. 2009 Dec;37(22):7560-9. doi: 10.1093/nar/gkp835. Epub . |
'
