aspsirna4043 details

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ASPsiRNA information siRNA Id:aspsirna4043
siRNA Name: siRNA-40_12G
Mismatch information Wild siRNA (As strand 3'->5'):CCUUAGCGUCCGUAGUACC
ASP-siRNA (As strand 3'->5'):CCUUAGCGUCCGUAGUACC
Mismatch position in siRNA: G:G
Gene Information Gene Name solute carrier family 12 (potassium/chloride transporter), member 5 (SLC12A5)
Target Sequence (5'->3'):ATCGCAGGCATCATGGAGTCCTTCTGC
Wild allele (5'->3'):GGAAUCGCAGGCAUCAUGG
Mutant allele (5'->3'):GGAAUCGGAGGCAUCAUGG
Position of siRNA on target gene: 488-506
Respective Gene/Protein Resources GenBank Accession:NM_134363.1
Cytogenic location:20q13.12
Chromosomal coordinates:20:44,650,328-44,688,788
UniProt ID:Q9H2X9
HUGO ID:NA
Reference SNp(RefSNP): NA
Disease/Mutation information Target Mutation: NA
Matation type/variant: NA
Mutation at gene level: NA
Pathogenic status of mutation: NA
Disease:NA
Clinical Resources ClinVar ID:NA
KEGG disease ID:NA
OMIM ID: NA
COSMIC: SLC12A5
DECIPHER: SLC12A5
GeneTests: SLC12A5
ASP siRNA details Mutant allele (5'->3'): GGAAUCGGAGGCAUCAUGG
ASP-siRNA (As strand 3'->5'):CCUUAGCGUCCGUAGUACC
Percentage efficacy of ASP-siRNA for mutant allele:14
Wild allele (5'->3'): GGAAUCGCAGGCAUCAUGG
ASP-siRNA (As strand 3'->5'):CCUUAGCGUCCGUAGUACC
Percentage efficacy of ASP-siRNA for wild allele:81
Relative difference:67
Wild siRNA details Wild allele (5'->3'): GGAAUCGCAGGCAUCAUGG
Wild siRNA (As strand 3'->5'):CCUUAGCGUCCGUAGUACC
Percentage efficacy of wild siRNA for wild allele:89
Wild allele (5'->3'): GGAAUCGCAGGCAUCAUGG
ASP-siRNA (As strand 3'->5'):CCUUAGCGUCCGUAGUACC
Percentage efficacy of Wild sirna for mutant allele:79
Relative difference :10
General information Mismatch incorporated in siRNA/Target:siRNA
Cell-line used: HEK293
Experimental technique used: RT-PCR, Flourescence microscopy
Transfection method: TriZol reagent
siRNA expression method: Genepharma, Proligo Sigma
Post-transfection duration: 24 hours
Concentration used:13nM
Reference:19815667
Delivery method:Transfection
Article title:Profiling of mismatch discrimination in RNAi enabled rational design of allele-specific siRNAs.
Authors:Huang H, Qiao R, Zhao D, Zhang T, Li Y, Yi F, Lai F, Hong J, Ding X, Yang Z, Zhang L, Du Q, Liang Z.
Journal Reference:Nucleic Acids Res. 2009 Dec;37(22):7560-9. doi: 10.1093/nar/gkp835. Epub .