aspsirna4080 details

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ASPsiRNA information siRNA Id:aspsirna4080
siRNA Name: siRNA-58_12A
Mismatch information Wild siRNA (As strand 3'->5'):CGUCCAGAGACAGUGCGAA
ASP-siRNA (As strand 3'->5'):CGUCCAGAGACAGUGCGAA
Mismatch position in siRNA: A:A
Gene Information Gene Name Chemokine (C-C motif) ligand 2 (CCL2)
Target Sequence (5'->3'):CCACTATGCAGGTCTCTGTCACGCTTCTGGGCCTGT
Wild allele (5'->3'):GCAGGUCUCUGUCACGCUU
Mutant allele (5'->3'):GCAGGUCACUGUCACGCUU
Position of siRNA on target gene: 78-96
Respective Gene/Protein Resources GenBank Accession:NM_031530.1
Cytogenic location:17q12
Chromosomal coordinates:17:32,582,295-32,584,221
UniProt ID:P13500
HUGO ID:NA
Reference SNp(RefSNP): NA
Disease/Mutation information Target Mutation: NA
Matation type/variant: NA
Mutation at gene level: NA
Pathogenic status of mutation: NA
Disease:NA
Clinical Resources ClinVar ID:NA
KEGG disease ID:NA
OMIM ID: NA
COSMIC: CCL2
DECIPHER: CCL2
GeneTests: CCL2
ASP siRNA details Mutant allele (5'->3'): GCAGGUCACUGUCACGCUU
ASP-siRNA (As strand 3'->5'):CGUCCAGAGACAGUGCGAA
Percentage efficacy of ASP-siRNA for mutant allele:90
Wild allele (5'->3'): GCAGGUCUCUGUCACGCUU
ASP-siRNA (As strand 3'->5'):CGUCCAGAGACAGUGCGAA
Percentage efficacy of ASP-siRNA for wild allele:91
Relative difference:1
Wild siRNA details Wild allele (5'->3'): GCAGGUCUCUGUCACGCUU
Wild siRNA (As strand 3'->5'):CGUCCAGAGACAGUGCGAA
Percentage efficacy of wild siRNA for wild allele:89
Wild allele (5'->3'): GCAGGUCUCUGUCACGCUU
ASP-siRNA (As strand 3'->5'):CGUCCAGAGACAGUGCGAA
Percentage efficacy of Wild sirna for mutant allele:83
Relative difference :6
General information Mismatch incorporated in siRNA/Target:siRNA
Cell-line used: HEK293
Experimental technique used: RT-PCR, Flourescence microscopy
Transfection method: TriZol reagent
siRNA expression method: Genepharma, Proligo Sigma
Post-transfection duration: 24 hours
Concentration used:13nM
Reference:19815667
Delivery method:Transfection
Article title:Profiling of mismatch discrimination in RNAi enabled rational design of allele-specific siRNAs.
Authors:Huang H, Qiao R, Zhao D, Zhang T, Li Y, Yi F, Lai F, Hong J, Ding X, Yang Z, Zhang L, Du Q, Liang Z.
Journal Reference:Nucleic Acids Res. 2009 Dec;37(22):7560-9. doi: 10.1093/nar/gkp835. Epub .