virsi1125 details

siRNA Id:virsi1125
Sense Sequence: aagaggacucuuggacucuc
Length: 20
GC Content (%):50
Virus Name:Hepatitis B Virus [HBV]
Family of Virus:Hepadnaviridae
Virus Strain:ayw
Target Gene:X
Genbank Accession: U95551
Starting Position 1658
Ending Position: 1677
Cell Line: HepG2.2.15
Transfection Method: Lipofectamine 2000
Incubation Time (Hours): 48
siRNA design algorithm used: Ambion
PubMed:19026173
Target Object (mRNA,Protein,etc): Protein
Silencing Efficacy :51
Structure:
siRNA sequence matching with Hepatitis B Virus [HBV] reference genome:
Virus Load % inhibition:27
Virus Load Method:Real-Time PCR
Target mRNA1:S/P mRNA
mRNA1 % inhibition:68
mRNA1 Detection Method:Real Time RT-PCR
Target mRNA2:C/P mRNA
mRNA2 % inhibition :83
mRNA2 Detection Method:Real Time RT-PCR
Protein1:HBeAg
Protein1 % inhibition:65
Protein1 Detection Method:ELISA
Protein2:HBsAg
Protein2 % inhibition :51
Protein2 Detection Method:ELISA

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