siRNA Id: | virsi1125 |
Sense Sequence: | aagaggacucuuggacucuc |
Length: | 20 |
GC Content (%): | 50 |
Virus Name: | Hepatitis B Virus [HBV] |
Family of Virus: | Hepadnaviridae |
Virus Strain: | ayw |
Target Gene: | X |
Genbank Accession: | U95551 |
Starting Position | 1658 |
Ending Position: | 1677 |
Cell Line: | HepG2.2.15 |
Transfection Method: | Lipofectamine 2000 |
Incubation Time (Hours): | 48 |
siRNA design algorithm used: | Ambion |
PubMed: | 19026173 |
Target Object (mRNA,Protein,etc): | Protein |
Silencing Efficacy : | 51 |
Structure: | |
siRNA sequence matching with Hepatitis B Virus [HBV] reference genome: | |
Virus Load % inhibition: | 27 |
Virus Load Method: | Real-Time PCR |
Target mRNA1: | S/P mRNA |
mRNA1 % inhibition: | 68 |
mRNA1 Detection Method: | Real Time RT-PCR |
Target mRNA2: | C/P mRNA |
mRNA2 % inhibition : | 83 |
mRNA2 Detection Method: | Real Time RT-PCR |
Protein1: | HBeAg |
Protein1 % inhibition: | 65 |
Protein1 Detection Method: | ELISA |
Protein2: | HBsAg |
Protein2 % inhibition : | 51 |
Protein2 Detection Method: | ELISA |
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