siRNA Id: | virsi1347 |
Sense Sequence: | gggcuaucaaccuauagau |
Length: | 19 |
GC Content (%): | 42 |
Virus Name: | SARS Coronavirus |
Family of Virus: | Coronaviridae |
Virus Strain: | Urbani |
Target Gene: | Spike |
Genbank Accession: | AY278741 |
Starting Position | 22085 |
Ending Position: | 22103 |
Cell Line: | Vero E6 |
Transfection Method: | Lipofectamine 2000 |
Incubation Time (Hours): | 48 |
siRNA Expression Method: | Chemically Synthesized |
siRNA design algorithm used: | Elbashir |
PubMed: | 15652970 |
Target Object (mRNA,Protein,etc): | RNA |
Silencing Efficacy : | 90 |
Structure: | |
siRNA sequence matching with SARS Coronavirus reference genome: | |
RNA % inhibition: | 90 |
RNA Detection method: | RT-PCR |
Protein1 % inhibition: | 64 |
Protein1 Detection Method: | Western Blotting |
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