siRNA Id: | virsi2192 |
Sense Sequence: | gugggucaucauugacgca |
Length: | 19 |
GC Content (%): | 53 |
Virus Name: | Hepatitis E Virus [HEV] |
Family of Virus: | Hepeviridae |
Target Gene: | Helicase |
Genbank Accession: | AF076239 |
Cell Line: | HepG2 |
Concentration: | 3 μg Level Per 35 mm Petri Dish (Corning) |
Transfection Method: | Lipofectamine 2000 / FuGENE 6 |
Incubation Time (Hours): | 72 |
siRNA Expression Method: | Chemically Synthesized |
siRNA design algorithm used: | Ambion |
PubMed: | 20105445 |
Target Object (mRNA,Protein,etc): | Protein |
Silencing Efficacy : | 70 |
Structure: | ![]() |
siRNA sequence matching with Hepatitis E Virus [HEV] reference genome: | ![]() |
Target mRNA1: | Helicase |
mRNA1 % inhibition: | 88 |
mRNA1 Detection Method: | RT-PCR |
Protein1: | Helicase |
Protein1 % inhibition: | 70 |
Protein1 Detection Method: | Luciferase Assay |
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