virsi2194 details

siRNA Id:virsi2194
Sense Sequence: ccugauuggcaugcuacag
Length: 19
GC Content (%):53
Virus Name:Hepatitis E Virus [HEV]
Family of Virus:Hepeviridae
Target Gene:Replicase
Genbank Accession: AF076239
Cell Line: HepG2
Concentration: 3 μg Level Per 35 mm Petri Dish (Corning)
Transfection Method: Lipofectamine 2000 / FuGENE 6
Incubation Time (Hours): 72
siRNA Expression Method: Chemically Synthesized
siRNA design algorithm used: Ambion
PubMed:20105445
Target Object (mRNA,Protein,etc): Protein
Silencing Efficacy :75
Structure:
siRNA sequence matching with Hepatitis E Virus [HEV] reference genome:
Target mRNA1:Replicase
mRNA1 % inhibition:88
mRNA1 Detection Method:RT-PCR
Protein1:Replicase
Protein1 % inhibition:75
Protein1 Detection Method:Luciferase Assay

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