virsi2195 details

siRNA Id:virsi2195
Sense Sequence: cucgggaguuauaguuuau
Length: 19
GC Content (%):37
Virus Name:Hepatitis E Virus [HEV]
Family of Virus:Hepeviridae
Target Gene:3CAE
Genbank Accession: AF076239
Cell Line: HepG2
Concentration: 3 μg Level Per 35 mm Petri Dish (Corning)
Transfection Method: Lipofectamine 2000 / FuGENE 6
Incubation Time (Hours): 72
siRNA Expression Method: Chemically Synthesized
siRNA design algorithm used: Ambion
PubMed:20105445
Target Object (mRNA,Protein,etc): Protein
Silencing Efficacy :82
Structure:
siRNA sequence matching with Hepatitis E Virus [HEV] reference genome:
Target mRNA1:3CAE Region
mRNA1 % inhibition:93
mRNA1 Detection Method:RT-PCR
Protein1:3CAE Region
Protein1 % inhibition:82
Protein1 Detection Method:Luciferase Assay

.
.
.
.
.
.
.
.
.
.
.

.

.

.