Automatic recommendation
OK - siRNA is specific for both sense and antisense (guide) strands
10 hits were found for GUGGUGGACUUCUCUCAAU (5 guide strand hits and 5 sense strand hits.)
Hits to Guide strand
| Accession | Gene | Start | End | Details of mismatches | Spec. Score | Mismatches | Contiguous | Jackson style specificity | Hits similar gene | HSP Score | HSP % Identity | HSP length | Gene Length | ||||||||||||||||||
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | |||||||||||||
| NM_000833 | GRIN2A | 1871 | 1889 | G | U | G | G | U | G | G | A | C | U | U | C | U | C | U | g | u | g | c | 42 | 4 | 15 | c | false | <90% | 6293 | ||
| NM_005907 | MAN1A1 | 1299 | 1317 | u | U | G | G | U | G | G | A | C | U | a | C | U | C | U | C | A | g | c | 29 | 4 | 9 | c | false | <90% | 4139 | ||
| XM_001125704 | LOC727778 | 1173 | 1191 | G | U | G | c | U | G | G | A | g | U | U | C | U | C | U | C | A | A | g | -1 | 3 | 9 | bc | false | <90% | 2506 | ||
| XM_001125757 | LOC727778 | 1173 | 1191 | G | U | G | c | U | G | G | A | g | U | U | C | U | C | U | C | A | A | g | -1 | 3 | 9 | bc | false | <90% | 4271 | ||
| XM_001125727 | LOC727778 | 1173 | 1191 | G | U | G | c | U | G | G | A | g | U | U | C | U | C | U | C | A | A | g | -1 | 3 | 9 | bc | false | <90% | 4332 | ||
Hits to Sense strand
| Accession | Gene | Start | End | Details of mismatches | Spec. Score | Mismatches | Contiguous | Jackson style specificity | Hits similar gene | HSP Score | HSP % Identity | HSP length | Gene Length | ||||||||||||||||||
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | |||||||||||||
| NM_024657 | MORC4 | 2095 | 2113 | u | c | U | G | A | G | A | G | A | A | G | U | a | C | A | C | C | A | C | 76 | 3 | 10 | false | <90% | 3815 | |||
| NM_016639 | TNFRSF12A | 360 | 378 | g | g | a | G | A | G | A | G | A | A | G | U | u | C | A | C | C | A | C | 56 | 4 | 9 | false | <90% | 998 | |||
| NM_033226 | ABCC12 | 1763 | 1781 | A | g | U | G | A | G | A | G | g | A | G | U | C | C | A | C | C | A | g | 23 | 3 | 9 | bc | false | <90% | 5168 | ||
| NM_173647 | RNF149 | 1167 | 1185 | g | a | U | G | A | G | A | G | c | A | G | U | C | C | A | C | C | A | u | 21 | 4 | 9 | bc | false | <90% | 2452 | ||
| NM_002689 | POLA2 | 1617 | 1635 | A | U | U | G | A | G | A | G | A | u | G | U | g | C | A | C | C | A | u | 1 | 3 | 9 | false | <90% | 2514 | |||
3 prime UTR Seed matches
Hits to same gene : 0
Hits to other genes : 3452
View matched accessions
3 prime seed match details
Immunostimulatory motif search results
UGUGU - Judge et al. Sequence-dependent stimulation of the mammalian innate immune response by synthetic siRNA. Nat Biotechnol. 2005 Apr;23(4):457-62. (PMID: 15778705)
No Immunostimulatory motif found (UGUGU).
GUCCUUCAA - Hornung et al. Sequence-specific potent induction of IFN-alpha by short interfering RNA in plasmacytoid dendritic cells through TLR7. Nat Med. 2005 Mar;11(3):263-70. Epub 2005 Feb 20. (PMID: 15723075)
No Immunostimulatory motif found (GUCCUUCAA).
Settings used for search
Scores were calculated using scoring scheme SS1
Using AVG scoring mode.
Hit scores
| Parameter | Spec. Score cut-off | Colour | Comment |
| Hit to same gene | Colour | Hits to the same accession or genes with 100 percent identity (i.e. likely transcript variants). It is advisable to always check the genes hit. | |
| High scoring hit | ≥80 | Colour | Hits are very likely to cause an off-target effect |
| Medium scoring hit | <80¸≥40 | Colour | Hits with a small chance of causing an off target effect. |
| Low scoring hit | Colour | Hits very unlikely to cause an off-target effect |
The mismatches are colour coded based on the weighting of that position.
| Cut-off | Colour |
| Match | Colour |
| Wobble base pairs (G-U) | Colour |
| Critical bases (none) | Colour |
| 0.6 bits | Colour |
| 0.4 bits | Colour |
| 0.2 bits | Colour |
SpecificityServer is designed to help you identify potential non-specific matches to your siRNA. It incorporates the latest information about non-specific matches (sequence-specific only).
Designed by: Alistair Chalk, Quan Du, Zicai Liang and Erik L.L. Sonnhammer