Predicted Off-targets for siRNA "ggucuuacauaagaggacu" Using siRNA SpecificityServer

Specificity Server [Result page v1.0]


SpecificityServer - An siRNA design program for determining siRNA specificity

Automatic recommendation
OK - siRNA is specific for both sense and antisense (guide) strands

5 hits were found for GGUCUUACAUAAGAGGACU (3 guide strand hits and 2 sense strand hits.)

Hits to Guide strand

AccessionGeneStartEndDetails of mismatchesSpec.
Score
MismatchesContiguousJackson style
specificity
Hits similar
gene
HSP
Score
HSP %
Identity
HSP lengthGene Length
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19
NR_003366 ANKRD20B 63140 63158 uuUCUUACAUAAGAGuACa55 4 13 c false   <90%   96524
XM_290712 MGC46336 175 193 cGUCUUACAUuAGAGaACU10 3 9 c false   <90%   1765
XM_937979 MGC46336 175 193 cGUCUUACAUuAGAGaACU10 3 9 c false   <90%   1765


Hits to Sense strand



AccessionGeneStartEndDetails of mismatchesSpec.
Score
MismatchesContiguousJackson style
specificity
Hits similar
gene
HSP
Score
HSP %
Identity
HSP lengthGene Length
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19
NM_001040274 NO145 929 947 uGUCaUCUUAUGaAAGACC36 3 7   false   <90%   3139
NM_145753 PHLDB2 1804 1822 AGUCCUCUUAUcUAAGuau35 4 11   false   <90%   5491


3 prime UTR Seed matches

Hits to same gene : 0
Hits to other genes : 1089
View matched accessions

3 prime seed match details

AccessionGene nameHits to this gene
NM_0010986383
NM_005504BCAT12
NM_033055HIAT12
NM_001039127ZNF7182
NM_022160DMRTA12
NM_175856CHSY-22
NM_145298APOBEC3F2
NM_004805POLR2D2
NM_023077C1orf1632
NM_005160ADRBK22
NM_006581FUT92
NM_007050PTPRT2
NM_133170PTPRT2
NM_018085IPO92
NM_014911AAK12
NM_206853QKI2
NM_206854QKI2
NM_206855QKI2
NM_007175ERLIN22
NM_003240LEFTY22
NM_001007279RASL10A2
NM_001830CLCN42
NM_002401MAP3K32
NM_203351MAP3K32
NM_003112SP42
NM_022166XYLT12
XM_001125986LOC2838242
NM_0330262
NM_198836ACACA2
NM_198837ACACA2
NM_198834ACACA2
NM_198838ACACA2
NM_198839ACACA2
NM_181521CMTM42
NM_002581PAPPA2
NM_001128AP1G12
NM_001030007AP1G12
NM_006212PFKFB22
NM_001013615C1orf1902
NM_001682ATP2B12
NM_001001323ATP2B12
NM_000390CHM2
NM_006584CCT6B1
NM_005849IGSF61
NM_003747TNKS1
NM_004394DAP1
NM_173496MPP71
NM_016009SH3GLB11
NM_007032TRIOBP1
NM_001039141TRIOBP1
NM_020870SH3RF11
NM_153346CXorf201
NM_006060IKZF11
NM_001039585PTGFR1
NM_000959PTGFR1
XM_927706LOC6445911
XM_927767LOC6535051
XM_928338LOC6535981
NM_014646LPIN21
NM_152342CDYL21
NM_005297MCHR11
NM_005259GDF81
NM_145341PDCD41
NM_014456PDCD41
NM_152573RASEF1
NM_012223MYO1B1
NM_001018108SERF21
NM_0010987181
XM_930068LOC6538091
XM_926086LOC6531141
NM_017688BSPRY1
NM_006472TXNIP1
NM_003980MAP71
NM_014899RHOBTB31
NM_001039618CREBZF1
NM_032435KIAA18041
NM_001079526IKZF21
NM_016260IKZF21
NM_018195C11orf571
NM_001082969C11orf571
NM_001082970C11orf571
NM_021132PPP3CB1
XM_001134281LOC7301391
XM_001134203LOC7322631
NM_000115EDNRB1
NM_175607CNTN41
NM_175612CNTN41
NM_175724IL5RA1
NM_175727IL5RA1
NM_015471NSL11
NM_001042549NSL11
NM_030755TXNDC11
NM_002417MKI671
NM_001919DCI1
NM_033122C4orf351
NM_019555ARHGEF31
NM_024312GNPTAB1
NM_015221DNMBP1
NM_006121KRT11
NM_031473IFT811
NM_000052ATP7A1
NM_001077691ALG91
NM_001077692ALG91
NM_024740ALG91
NM_001077690ALG91
NM_004360CDH11
NM_000046ARSB1
NM_198337INSIG11
NM_005542INSIG11
NM_198336INSIG11
NM_181809BMP8A1
NM_003489NRIP11
NM_001039547GK51
XM_936833LOC6441961
XM_927389LOC6441961
NM_018362LIN7C1
NM_181527NAT51
NM_181528NAT51
NM_016100NAT51
NM_0175521
NM_001584MPPED21
NM_001008539SLC7A21
NM_003046SLC7A21
NM_016201AMOTL21
NM_001009993LOC1300741
NM_012333MYCBP1
NM_001018082ADIG1
NM_001013660FRRS11
NM_000208INSR1
NM_001079817INSR1
NM_005385NKTR1
NM_032446MEGF101
NM_018243SEPT111
NM_020772NUFIP21
NM_003620PPM1D1
NM_032810ATAD11
NM_198274SMYD11
NM_001010846SHE1
NM_021100NFS11
NM_005019PDE1A1
NM_001032363C1orf1511
NM_015595SGEF1
NM_004559YBX11
NM_001001662ZNF7821
NM_0011111131
NM_005339HIP21
NM_0011111121
NM_017777MKS11
NM_021238FAM60A1
NM_080591PTGS11
NM_000962PTGS11
NM_013255MKLN11
XM_001128243LOC7287051
NM_012234RYBP1
XM_944991C14orf821
XM_498578C14orf821
NM_004066CETN11
NM_0010984021
NM_0010984031
NM_020727ZNF2951
NM_016255FAM8A11
NM_022041GAN1
NM_194434VAPA1
NM_003574VAPA1
NM_016026RDH111
NM_001040429PCDH171
NM_020813ZNF4711
NM_173562KCTD201
NM_003734AOC31
NM_207171POGZ1
NM_015100POGZ1
NM_173359EIF4E31
XM_001129292MBOAT21
NM_021629GNB41
NM_005336HDLBP1
NM_203346HDLBP1
NM_145687MAP4K41
NM_145686MAP4K41
NM_004834MAP4K41
NM_178457C20orf1741
NM_1827627A51
NM_014674EDEM11
NM_018696ELAC11
NM_001040653ZXDC1
NM_006764IFRD21
XM_939981LOC6469761
XM_929947LOC6469761
NM_018111FLJ104901
NM_006802SF3A31
NM_005725TSPAN21
NM_170774RASSF21
NM_014737RASSF21
NM_001080745DGKG1
NM_001346DGKG1
NM_001080744DGKG1
NM_001025233CLLU11
NM_000868HTR2C1
NM_003161RPS6KB11
NM_015158ANKRD151
NM_153186ANKRD151
NM_006701TXNL4A1
NM_0209101
NM_139276STAT31
NM_003150STAT31
NM_213662STAT31
NM_176825SULT1C21
NM_001056SULT1C21
NM_005810KLRG11
NM_020448NPAL31
NM_001005751LOC3876801
NM_0182321
NM_015262FAM21C1
XM_370557LOC6534501
NM_001040146CTF81
NM_001039690CTF81
NM_001040144CTF81
NM_002148HOXD101
NM_000391TPP11
NM_133478SLC4A51
NM_017635SUV420H11
NM_004612TGFBR11
NM_017772TBC1D22B1
NM_018958C15orf21
NM_012419RGS171
NM_004449ERG1
NM_182918ERG1
XM_001130790KIAA18811
NM_001080400KIAA18811
XM_001129896LOC7315081
NM_000442PECAM11
NM_004782SNAP291
NM_002030FPRL21
NM_080625C20orf1601
NM_032873STS-11
NM_005399PRKAB21
NM_207404ZNF6621
NM_001042631LOC6440961
NM_152475ZNF4171
NM_020766PCDH191
NM_0011052431
NM_014703VPRBP1
NM_033389SSH21
NM_001018111PODXL1
NM_005397PODXL1
NM_004560ROR21
NM_170782KCNN31
NM_002249KCNN31
NM_004741NOLC11
NM_144963FAM91A11
XM_001126720PDZK11
XM_001126677PDZK11
XM_001126710PDZK11
XM_001131007LOC7296781
XM_001127177LOC7307801
XM_001127275LOC7308031
XM_001133269LOC7302491
NM_181782NCOA71
NM_173073SLC35C21
NM_173179SLC35C21
NM_015945SLC35C21
NM_032828ZNF5871
NM_021137TNFAIP11
NM_138292ATM1
NM_000051ATM1
NM_004078CSRP11
NM_172005WFDC131
NM_000096CP1
NM_182511CBLN21
NM_001040159SPOCK31
NM_016950SPOCK31
NM_052907TMEM132B1
NM_017952PTCD31
NM_030923TMEM1631
NM_004319ASTN11
NM_133646ZAK1
NM_0010853571
NM_181780BTLA1
NM_017707DDEFL11
NM_0011055531
NM_0011055541
NM_018300ZNF831
NM_0011055501
NM_0011055511
NM_0011055521
NM_0011055491
NM_020387RAB251
NM_017755NSUN21
NM_000167GK1
NM_203391GK1
NM_032783CBR41
NM_020348CNNM11
NM_018945PDE7B1
NM_000393COL5A21
NM_004148NINJ11
NM_0011004211
NM_0011004201
NM_017447C21orf911
NM_152705POLR1D1
NM_015058KIAA05641
NM_176814ZNF8001
NM_177424STX121
NM_152417TMEM681
NM_014033METTL7A1
NM_000242MBL21
NM_181876PPP2R2C1
NM_020416PPP2R2C1
NM_003455ZNF2021
NM_020648TWSG11
NM_020466LYRM21
NM_004852ONECUT21
NM_001040424PRDM151
NM_022549FEZ11
NM_014959CARD81
NM_007086WDHD11
NM_001008396WDHD11
NM_003994KITLG1
NM_000899KITLG1
NM_018963BRWD11
NM_001029863C6orf1201
NM_080928ASB151
NM_015886PI151
NM_001792CDH21
NM_022840METTL41
NM_1736151
NM_014157CCDC1131
NM_152916EMR21
NM_152918EMR21
NM_152917EMR21
NM_152920EMR21
NM_152921EMR21
NM_013447EMR21
NM_152919EMR21
NM_006631ZNF2661
NM_198058ZNF2661
NM_0011003981
NM_015085GARNL41
NM_015190DNAJC91
NM_006965ZNF241
NM_001005502CPM1
NM_198320CPM1
NM_001874CPM1
NM_139166ABRA1
NM_000430PAFAH1B11
NM_025202EFHD11
NM_015316PPP1R13B1
NM_153365TAPT11
NM_002959SORT11
NM_014117C16orf721
NM_001742CALCR1
NM_152551C6orf1511
NM_001025096PSMA81
NM_001025097PSMA81
NM_144662PSMA81
NM_003799RNMT1
NM_018241TMEM341
XM_942780SYNPO21
NM_181707C17orf641
NM_001008388ZCD21
NM_021135RPS6KA21
NM_001006932RPS6KA21
NM_002061GCLM1
NM_001013842C8orf581
NM_006195PBX31
NM_182791CCDC331
NM_014504RABGEF11
NM_003137SRPK11
NM_004426PHC11
XM_934166LOC6534411
XM_945184LOC6534411
NM_019863F81
NM_000132F81
NM_001730KLF51
NM_022482GZF11
NM_012193FZD41
NM_032511C6orf1681
NM_198267ING31
NM_033326SOX61
NM_017508SOX61
XM_495908DDX121
NM_183419RNF191
NM_015435RNF191
XM_001129796LOC6455131
NM_080283ABCA91
NM_024833ZNF6711
NM_013252CLEC5A1
NM_005409CXCL111
NM_006527SLBP1
NM_198243ASB71
NM_018933PCDHB131
NM_178425HDAC91
NM_178423HDAC91
NM_017705PAQR51
NM_0011045541
NM_152263TPM31
NM_004444EPHB41
NM_001748CAPN21
NM_014423AFF41
NM_000813GABRB21
NM_021911GABRB21
NM_020844KIAA14561
NM_0010996771
NM_145167PIGM1
NM_019041MTRF1L1
NM_018847KLHL91
NM_018260ZNF7011
NM_020463SMEK21
NM_020630RET1
NM_182503DEADC11
NM_005449FAIM31
NM_152695ZNF4491
NM_199131VAX11
NM_153456HS6ST31
NM_000927ABCB11
NM_153714C10orf671
NM_198968DZIP11
NM_014934DZIP11
NM_006029PNMA11
NM_181701QSOX21
NM_001196BID1
NM_197967BID1
NM_197966BID1
NM_138468ICA1L1
NM_005121THRAP11
XM_942121LOC6458431
XM_930419LOC6458431
NM_018290PGM21
NM_001007024GOSR11
NM_001007025GOSR11
NM_004871GOSR11
XM_001133059LOC7287721
XM_001133074LOC7287721
XM_001133066LOC7287721
NM_145041TMEM106A1
NM_138288C14orf1471
NM_021959PPP1R111
NM_012325MAPRE11
NM_015199ANKRD281
NM_014553TFCP2L11
XM_379623FLJ412001
XM_943473FLJ412001
NM_020724RNF1501
NM_000875IGF1R1
NM_015697COQ21
NM_182749N6AMT11
NM_013240N6AMT11
NM_002025AFF21
NM_004686MTMR71
NM_015002FBXO211
NM_033624FBXO211
NM_004621TRPC61
NM_005068SIM11
NM_018951HOXA101
NM_153715HOXA101
NM_001440EXTL31
NM_0010992711
NM_152408C5orf371
NM_031281FCRL51
NM_001453FOXC11
NM_003144SSR11
NM_015879ST8SIA31
NM_019625ABCB91
NM_203444ABCB91
NM_019624ABCB91
NM_080592C2orf281
NM_016085C2orf281
NM_022474MPP51
NM_178542C17orf651
NM_001010980C1orf1301
NM_000411HLCS1
NM_018463ITFG21
NM_001012965KLK61
NM_002774KLK61
NM_001012964KLK61
NM_013406DHPS1
NM_001930DHPS1
NM_013407DHPS1
NM_020161DKFZp547H0251
NM_000990RPL27A1
NM_014276RBPJL1
NM_178554KY1
NM_020853KIAA14671
NM_023112OTUB21
NM_003644GAS71
NM_201432GAS71
NM_201433GAS71
NM_003681PDXK1
NM_148905OSBPL91
NM_148904OSBPL91
NM_148906OSBPL91
NM_148907OSBPL91
NM_148908OSBPL91
NM_024586OSBPL91
NM_148909OSBPL91
NM_0010996521
NM_021259TMEM81
NM_002390ADAM111
NM_012280FTSJ11
NM_177439FTSJ11
NM_177434FTSJ11
NM_206866BACH11
NM_001186BACH11
NM_080764SUHW21
NM_025031FLJ210751
NM_002547OPHN11
NM_032846RAB2B1
NM_020135WRNIP11
NM_130395WRNIP11
NM_015162ACSBG11
NM_003304TRPC11
NM_001012427FOXP41
NM_138457FOXP41
NM_001012426FOXP41
NM_019009TOLLIP1
NM_003099SNX11
NM_152826SNX11
NM_148955SNX11
NM_005895GOLGA31
NM_003409ZFP1611
NM_021033RAP2A1
NM_174912FAAH21
XM_941706LOC6490091
NM_145250RNASE111
NM_019593KIAA14341
NM_012429SEC14L21
NM_148911CHRFAM7A1
NM_139320CHRFAM7A1
NM_145176SLC2A121
NM_004775B4GALT61
NM_020666CLK41
NM_198066GNPNAT11
NM_007345ZNF2361
NM_004438EPHA41
XM_928745LOC6434751
XM_940123LOC6434751
NM_032151PCBD21
XM_001133948LOC7318551
NM_020859SHROOM31
NM_004862LITAF1
NM_007176C14orf11
NM_001254CDC61
NM_016040TMED51
NM_001011709PNLIPRP31
NM_001076778FAM107A1
NM_007177FAM107A1
NM_020644TMEM9B1
NM_012444SPO111
NM_198265SPO111
NM_005189CBX21
NM_001018058TFEC1
NM_012252TFEC1
NM_020974SCUBE21
XM_001133863LOC7297371
NM_002851PTPRZ11
NM_004590CCL161
NM_014873LPGAT11
NM_004109FDX11
NM_030918SNX271
NM_133367PAQR81
NM_003253TIAM11
NM_015659RSL1D11
NM_018100EFHC11
NM_152754SEMA3D1
NM_020957PCDHB161
NM_000134FABP21
NM_000021PSEN11
NM_001024688NBN1
NM_002485NBN1
NM_021141XRCC51
NM_015238WWC11
NM_003787NOL41
NM_0208231
NM_020443NAV11
NM_022778CCDC211
NM_014772KIAA04271
NM_005657TP53BP11
NM_020385REXO41
NM_138400NOM11
NM_153688ZFP11
NM_001039697SNAPC31
NM_033419PERLD11
NM_016263FZR11
NM_172071RC3H11
NM_0010996421
NM_018348FLJ111711
NM_205852CLEC12B1
XM_166659OTUD11
XM_001134465OTUD11
NM_001271CHD21
NM_052957ACRC1
NM_001040709SYPL21
NM_022756C1orf1491
NM_005857ZMPSTE241
NM_016146TRAPPC41
NM_004849ATG51
NM_015576ERC21
XM_941162LOC4009501
XM_376049LOC4009501
NM_002998SDC21
NM_025074FRAS11
XM_001128081LOC4402681
XM_496063LOC4402681
NM_003908EIF2S21
NM_001007563IGFBPL11
NM_001017981RNF2151
NM_018200HMG20A1
NM_005020PDE1C1
NM_001082534CA101
NM_020178CA101
NM_001082533CA101
NM_152395NUDT161
NM_022353OSGEPL11
XM_001128291PARP81
NM_198715PTGER31
NM_012316KPNA61
NM_001003406CACNA1I1
NM_021096CACNA1I1
NM_144723ZMAT21
NM_020477ANK11
NM_020475ANK11
NM_000037ANK11
NM_020476ANK11
NM_020478ANK11
NM_020480ANK11
NM_001007070SDCBP1
NM_001007067SDCBP1
NM_005625SDCBP1
NM_001007069SDCBP1
NM_001007068SDCBP1
NM_032290ANKRD321
NM_020782KLHDC51
NM_172169CAMK2G1
NM_001222CAMK2G1
NM_172173CAMK2G1
NM_172171CAMK2G1
NM_172170CAMK2G1
NM_001008537KIAA20221
NM_020335VANGL21
NM_004487GOLGB11
NM_002299LCT1
NM_145280FAM119A1
NM_014733ZFYVE161
NM_0011052511
NM_001966EHHADH1
NM_005159ACTC11
NM_006694JTB1
XM_944531LOC6459491
XM_933289LOC6459491
NM_181472CMTM71
NM_138410CMTM71
NM_018932PCDHB121
NM_018940PCDHB71
NM_032826SLC35B41
NM_032825ZNF3821
NM_005095ZMYM41
NM_017515SLC35F21
NM_181458PAX31
NM_181460PAX31
NM_181457PAX31
NM_181459PAX31
NM_018662DISC11
NM_001012957DISC11
NM_0178921
NM_018047RBM221
NM_004598SPOCK11
NM_001077440BCLAF11
NM_001077441BCLAF11
NM_014739BCLAF11
NM_173851SLC30A81
XM_926382LOC6427551
NM_014015DEXI1
NM_0010853751
NM_015836WARS21
NM_201263WARS21
NM_207381TNFAIP8L31
NM_014786ARHGEF171
NM_005885MARCH61
NM_001042573FLJ218651
XM_938779LOC6539721
NM_007276CBX31
NM_016587CBX31
NM_024508ZBED21
NM_0151771
NM_032548ABTB11
NM_172028ABTB11
NM_172027ABTB11
NM_021813BACH21
NM_174953ATP2A31
NM_174956ATP2A31
NM_174954ATP2A31
NM_174957ATP2A31
NM_174958ATP2A31
NM_005173ATP2A31
NM_174955ATP2A31
NM_0151881
NM_181491SURF51
NM_001003407ABLIM11
NM_001003408ABLIM11
NM_002313ABLIM11
NM_006720ABLIM11
NM_001879MASP11
NM_021138TRAF21
NM_001003684UCRC1
NM_013387UCRC1
NM_138387G6PC31
NM_004200SYT71
XM_943686SYT71
NM_170672RASGRP31
NM_033081DIDO11
NM_020353PLSCR41
NM_001002026CLDN181
NM_016369CLDN181
NM_017643MBTD11
NM_005375MYB1
NM_018688BIN31
XM_001130733LOC7295941
XM_001131398LOC7319611
NM_015898ZBTB7A1
NM_153018ZFP31
NM_000332ATXN11
NM_001089ABCA31
NM_001040458ARTS-11
NM_021954GJA31
NM_005419STAT21
NM_016946F11R1
NM_018242FLJ108471
NM_001977ENPEP1
NM_176877INADL1
NM_144968RIBC11
NM_014293NPTXR1
NM_145321OSBPL31
NM_145320OSBPL31
NM_145322OSBPL31
NM_015550OSBPL31
XM_059166LOC1276021
XM_938194LOC1276021
NM_001729BTC1
NM_0011004001
NM_018196TMLHE1
NM_014342MTCH21
XM_499010LOC4410971
XM_943902LOC4410971
NM_0011045461
NM_148902TNFRSF181
NM_004195TNFRSF181
NM_020211RGMA1
NM_018427RRN31
NM_0149871
NM_003156STIM11
NM_153620HOXA11
NM_005522HOXA11
NM_006926SFTPA21
NM_0010986681
NM_021977SLC22A31
NM_001017440CALN11
NM_031468CALN11
NM_152624DCP21
NM_032595PPP1R9B1
NM_002364MAGEB21
NM_022744C16orf581
NM_004036ADCY31
NM_033516TPD52L31
NM_139157ST51
NM_005418ST51
NM_213618ST51
NM_173474NTAN11
NM_020458TTC7A1
NM_014964EPN21
NM_0011026641
NM_148921EPN21
NM_024046CAMKV1
NM_003557PIP5K1A1
NM_003449TRIM261
NM_020927KIAA15761
NM_006546IGF2BP11
NM_021045ZNF2481
NM_015166MLC11
NM_139202MLC11
NM_004420DUSP81
NM_012405ICMT1
NM_022465IKZF41
NM_001009611PRAMEF41
NM_001010890PRAMEF91
XM_497653LOC4418731
XM_928401LOC6453591
XM_001130065LOC7293681
NM_001013407PRAMEF51
NM_001010889PRAMEF61
NM_005927MFAP31
NM_001011703FAM125B1
NM_139245PPM1L1
NM_002147HOXB51
NM_175932PSMD131
NM_002817PSMD131
NM_147192DMBX11
NM_172225DMBX11
NM_002468MYD881
NM_002356MARCKS1
NM_032740SFT2D31
NM_178422PAQR71
NM_020116FSTL51
NM_002886RAP2B1
XM_001126630LOC7306351
XM_001133153LOC7302361
NM_003125SPRR1B1
XM_001127680SPRR1B1
NM_0011127361
NM_015224C3orf631
NM_013361ZNF2231
NM_012328DNAJB91
NM_001006620MAPKAP11
NM_001006619MAPKAP11
NM_001006621MAPKAP11
NM_024117MAPKAP11
NM_001006617MAPKAP11
NM_004220ZNF2131
NM_012423RPL13A1
NM_017671C20orf421
NM_013231FLRT21
NM_017438SETD41
NM_001008494ISX1
NM_133337FER1L31
NM_013451FER1L31
NM_001024647RAB3IP1
NM_022456RAB3IP1
NM_175624RAB3IP1
NM_175625RAB3IP1
NM_175623RAB3IP1
NM_152371C1orf931
NM_033505SELI1
NM_003274TMEM11
XM_938665USP6NL1
NM_001080491USP6NL1
XM_927409USP6NL1
XM_943800USP6NL1
NM_022131CLSTN21
NM_002816PSMD121
XM_001134072PSMD121
XM_001134070PSMD121
XM_001134073PSMD121
NM_183075CYP2U11
NM_030962SBF21
NM_002613PDPK11
NM_031268PDPK11
XM_001125875LOC7278181
XM_001133677LOC7292641
XM_001133686LOC7293551
XM_001133674LOC7292641
XM_001133681LOC7293551
XM_930448LOC6535501
NM_014289CAPN61
NM_032012C9orf51
NM_0010997341
NM_000173GP1BA1
NM_003012SFRP11
NM_020718USP311
NM_0190041
NM_006310NPEPPS1
NM_053282SH2D1B1
NM_207337AMN11
NM_020871LRCH21
NM_206909PSD31
NM_015310PSD31
NM_033020TRIM331
NM_015906TRIM331
NM_018403DCP1A1
NM_152233SNX61
NM_021249SNX61
NM_021795ELK41
NM_000367TPMT1
NM_032047B3GNT51
NM_015461ZNF5211
NM_001010987IFIT1L1
NM_001001664LOC3397451
NM_145165CHURC11
NM_004744LRAT1
NM_002858ABCD31
NM_006952UPK1B1
NM_033222PSIP11
NM_018098ECT21
NM_182607VSIG11
NM_031463HSDL11
NM_0010986121
NM_014813LRIG21
NM_147190LASS51
NM_032239LARP21
NM_177972TUB1
NM_003320TUB1
NM_054030MRGPRX21
NM_031200CCR91
NM_006641CCR91
NM_199138FAM123A1
NM_152704FAM123A1
NM_007183PKP31
XM_001129327PKP31
NM_004654USP9Y1
NM_001144AMFR1
NM_001690ATP6V1A1
NM_152609C1orf711
NM_032434ZNF5121
NM_024831TGS11
NM_014365HSPB81
NM_021982SEC24A1
NM_024828C9orf821
NM_052904KLHL321
NM_033255EPSTI11
NM_001002264EPSTI11
NM_004100EYA41
NM_172105EYA41
NM_172103EYA41
NM_017923MARCH11
NM_173495PTCHD11
NM_003281TNNI11
NM_004091E2F21
NM_014947FOXJ31
NM_138554TLR41
NM_207344SPRYD41
NM_001003712OSBPL81
NM_020841OSBPL81
NM_013955NOX11
NM_007052NOX11
NM_001439EXTL21
NM_001033025EXTL21
NM_001009184GRINA1
NM_000837GRINA1
NM_005171ATF11
NM_004057S100G1
NM_020784KIAA13441
NM_152470RNF1651
NM_006895HNMT1
NM_015621CCDC691
NM_0011045441
NM_0011045451
NM_017938FAM70A1
NM_004842AKAP71
NM_138633AKAP71
NM_016377AKAP71
NM_021229NTN41
NM_015172BAT2D11
NM_024792FAM57A1
NM_018480TMEM126B1
NM_033102SLC45A31
NM_194282LIN541
NM_005096ZMYM31
NM_201599ZMYM31
NM_173214NFAT51
NM_006599NFAT51
NM_138714NFAT51
NM_0011131781
NM_138713NFAT51
NM_001003937TSPYL61
NM_001031679MSRB31
NM_198080MSRB31
NM_002355M6PR1
NM_000865HTR1E1
NM_001759CCND21
NM_001002255SUMO41
NM_005465AKT31
NM_173460DEFB1191
NM_173510CCDC1171
NM_001105ACVR11
NM_001829CLCN31
NM_173872CLCN31
NM_003308TSPY11
XM_001127025LOC7284121
NM_022573TSPY21
XM_001126455LOC7281321
XM_001127004LOC7284031
XM_001126990LOC7283951
NM_015191SNF1LK21
NM_175907ZADH21
NM_021809TGIF21
NM_182758WDR721
XM_001127604NFATC2IP1
NM_032815NFATC2IP1
NM_006379SEMA3C1
NM_016243CYB5R11
NM_024329EFHD21
NM_016530RAB8B1
NM_012241SIRT51
NM_022826MARCH71
NM_012343NNT1
NM_182977NNT1
NM_001039770FLJ450321
NM_198525KIF71
NM_130438DYRK1A1
NM_101395DYRK1A1
NM_130436DYRK1A1
NM_001396DYRK1A1
NM_130437DYRK1A1
NM_152914MGC338941
NM_006734HIVEP21
NM_152414BHLHB51
NM_170609CRISP11
NM_001131CRISP11
NM_014550CARD101
NM_197939RNF1351
NM_001032283TMPO1
NM_001032284TMPO1
NM_057179TWIST21
NM_014862ARNT21
NM_014809KIAA03191
XM_001134027LOC7318891
XM_001134374LOC7301321
NM_004653JARID1D1
NM_005420SULT1E11
NM_152463EME11
NM_017686GDAP21
NM_022060ABHD41
NM_173548ZNF5841
NM_004863SPTLC21
NM_015447CAMSAP11
NM_000629IFNAR11
NM_021116ADCY11
NM_001031681CTNS1
NM_004937CTNS1
NM_017811UBE2R21
NM_001001732C10orf1301
NM_014257CLEC4M1
NM_214679CLEC4M1
NM_214678CLEC4M1
NM_214676CLEC4M1
NM_214677CLEC4M1
NM_032932RAB11FIP41
NM_144579SFXN51
NM_004088DNTT1
NM_001017520DNTT1
NM_024635MAK101
NM_178140PDZD21
NM_015387PREI31
NM_199482PREI31
NM_0011008191
NM_0207331
NM_013281FLRT31
NM_198391FLRT31
NM_001025073C3orf171
NM_015412C3orf171
NM_001025072C3orf171
NM_145173DIRAS11
NM_003566EEA11
NM_205855UNQ19401
NM_012460TIMM91
NM_005797EVA11
NM_0011008731
NM_182943PLOD21
NM_000935PLOD21
NM_016454TMEM851
NM_024306FA2H1
NM_020715PLEKHH11
NM_005740DNAL41
NM_003115UAP11
NM_182580CYB561D11
NM_002188IL131
NM_013233STK391
NM_022845CBFB1
NM_001755CBFB1
NM_003262TLOC11
NM_148170CTSC1
NM_022149MAGEF11
NM_0010839621
NM_003199TCF41
NM_020474GALNT11
NM_015294TRIM371
XM_001131844LOC7320971
XM_001126459LOC7280651
NM_138959VANGL11
NM_178450MARCH31
XM_001127871MARCH31
NM_017915C12orf481
NM_003795SNX31
NM_001735C51
NM_016598ZDHHC31
NM_001040167LFNG1
NM_020245TULP41
NM_001007466TULP41
NM_015257KIAA02861
NM_000038APC1
NM_0321731
XM_379636C9orf1221
XM_940165C9orf1221
NM_182916TRNT11
NM_0133181
NM_030650KIAA17151
NM_020482FHL51
NM_0011014211
NM_182609ZNF6771
NM_002750MAPK81
NM_139047MAPK81
NM_139046MAPK81
NM_139049MAPK81

Immunostimulatory motif search results


UGUGU - Judge et al. Sequence-dependent stimulation of the mammalian innate immune response by synthetic siRNA. Nat Biotechnol. 2005 Apr;23(4):457-62. (PMID: 15778705)
No Immunostimulatory motif found (UGUGU).

GUCCUUCAA - Hornung et al. Sequence-specific potent induction of IFN-alpha by short interfering RNA in plasmacytoid dendritic cells through TLR7. Nat Med. 2005 Mar;11(3):263-70. Epub 2005 Feb 20. (PMID: 15723075)
No Immunostimulatory motif found (GUCCUUCAA).



Settings used for search

Scores were calculated using scoring scheme SS1
Using AVG scoring mode.

Hit scores

ParameterSpec. Score cut-offColourComment
Hit to same geneColourHits to the same accession or genes with 100 percent identity (i.e. likely transcript variants). It is advisable to always check the genes hit.
High scoring hit≥80ColourHits are very likely to cause an off-target effect
Medium scoring hit<80¸≥40ColourHits with a small chance of causing an off target effect.
Low scoring hitColourHits very unlikely to cause an off-target effect

The mismatches are colour coded based on the weighting of that position.

Cut-offColour
MatchColour
Wobble base pairs (G-U)Colour
Critical bases (none)Colour
0.6 bitsColour
0.4 bitsColour
0.2 bitsColour

About SpecificityServer

SpecificityServer is designed to help you identify potential non-specific matches to your siRNA. It incorporates the latest information about non-specific matches (sequence-specific only).

Designed by: Alistair Chalk, Quan Du, Zicai Liang and Erik L.L. Sonnhammer


For more details about the algorithm "siRNA specificity searching incorporating mismatch tolerance data" Bioinformatics (2008) 24 (10): 1316-1317