Automatic recommendation
OK - siRNA is specific for both sense and antisense (guide) strands
25 hits were found for CCCGUCUGUGCCUUCUCAU (14 guide strand hits and 11 sense strand hits.)
Hits to Guide strand
Accession | Gene | Start | End | Details of mismatches | Spec. Score | Mismatches | Contiguous | Jackson style specificity | Hits similar gene | HSP Score | HSP % Identity | HSP length | Gene Length | ||||||||||||||||||
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | |||||||||||||
NM_001080436 | WTIP | 1454 | 1472 | a | C | C | G | U | C | U | G | U | G | C | C | U | g | C | U | C | A | a | 73 | 3 | 12 | c | false | <90% | 2204 | ||
XM_939855 | WTIP | 2384 | 2402 | a | C | C | G | U | C | U | G | U | G | C | C | U | g | C | U | C | A | a | 73 | 3 | 12 | c | false | <90% | 3134 | ||
NM_024870 | DEPDC2 | 3847 | 3865 | a | C | u | G | U | C | U | G | U | G | C | C | U | U | C | U | C | u | g | 70 | 4 | 14 | c | false | <90% | 5132 | ||
NM_152599 | FLJ35773 | 338 | 356 | C | C | C | G | U | C | U | G | U | G | C | C | U | U | C | c | u | g | g | 42 | 4 | 15 | c | false | <90% | 2151 | ||
NM_032491 | RFX4 | 1885 | 1903 | C | C | C | G | U | C | U | G | U | G | C | a | U | U | C | U | g | c | g | 35 | 4 | 11 | false | <90% | 3381 | |||
NM_213594 | RFX4 | 2127 | 2145 | C | C | C | G | U | C | U | G | U | G | C | a | U | U | C | U | g | c | g | 35 | 4 | 11 | false | <90% | 3623 | |||
NM_001001661 | ZNF425 | 2205 | 2223 | C | C | C | a | U | C | U | G | U | G | C | C | U | U | C | a | C | A | g | 32 | 3 | 11 | c | false | <90% | 3184 | ||
NM_001032395 | GPR126 | 2718 | 2736 | C | C | C | a | U | C | U | G | U | G | C | C | U | U | C | U | g | g | g | 32 | 4 | 12 | c | false | <90% | 6818 | ||
NM_001032394 | GPR126 | 2718 | 2736 | C | C | C | a | U | C | U | G | U | G | C | C | U | U | C | U | g | g | g | 32 | 4 | 12 | c | false | <90% | 6864 | ||
NM_198569 | GPR126 | 2802 | 2820 | C | C | C | a | U | C | U | G | U | G | C | C | U | U | C | U | g | g | g | 32 | 4 | 12 | c | false | <90% | 6902 | ||
NM_020455 | GPR126 | 2802 | 2820 | C | C | C | a | U | C | U | G | U | G | C | C | U | U | C | U | g | g | g | 32 | 4 | 12 | c | false | <90% | 6948 | ||
NM_025090 | USP36 | 4494 | 4512 | C | C | a | G | g | C | U | G | U | G | C | C | U | U | C | U | C | A | U | 28 | 2 | 14 | bc | false | <90% | 5234 | ||
XM_001131346 | LOC731941 | 1143 | 1161 | u | C | u | G | U | C | U | u | U | G | C | C | U | U | C | U | C | A | U | 16 | 3 | 11 | bc | false | <90% | 2044 | ||
NM_024067 | C7orf26 | 1356 | 1374 | C | C | C | G | U | g | U | G | U | G | C | C | U | U | C | U | g | c | g | 5 | 4 | 10 | c | false | <90% | 1913 |
Hits to Sense strand
Accession | Gene | Start | End | Details of mismatches | Spec. Score | Mismatches | Contiguous | Jackson style specificity | Hits similar gene | HSP Score | HSP % Identity | HSP length | Gene Length | ||||||||||||||||||
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | |||||||||||||
NM_079834 | SCAMP4 | 658 | 676 | u | c | c | A | G | A | A | G | G | C | A | C | A | G | A | C | G | G | a | 62 | 4 | 15 | bc | false | <90% | 2548 | ||
NM_152888 | COL22A1 | 3873 | 3891 | g | a | G | A | G | A | A | G | G | C | A | C | A | G | A | a | G | G | G | 60 | 3 | 13 | c | false | <90% | 6366 | ||
XM_001128037 | LOC728654 | 95 | 113 | A | U | G | A | G | A | A | G | G | C | A | C | A | G | c | C | c | a | G | 49 | 3 | 14 | c | false | <90% | 584 | ||
XM_001129553 | LOC731413 | 95 | 113 | A | U | G | A | G | A | A | G | G | C | A | C | A | G | c | C | c | a | G | 49 | 3 | 14 | c | false | <90% | 584 | ||
NM_015075 | IQSEC2 | 665 | 683 | A | U | G | A | G | A | A | G | G | C | A | C | A | G | A | a | c | c | c | 42 | 4 | 15 | c | false | <90% | 5248 | ||
XM_001132803 | LOC730175 | 156 | 174 | A | U | G | A | G | A | A | G | G | C | A | g | A | G | A | C | c | u | u | 35 | 4 | 11 | false | <90% | 216 | |||
XM_001131623 | LOC732020 | 156 | 174 | A | U | G | A | G | A | A | G | G | C | A | g | A | G | A | C | c | u | u | 35 | 4 | 11 | false | <90% | 216 | |||
NM_020198 | CCDC47 | 1101 | 1119 | g | g | G | A | G | A | A | G | u | C | A | C | A | G | A | C | G | G | a | 21 | 4 | 9 | bc | false | <90% | 3294 | ||
NM_020901 | KIAA1542 | 280 | 298 | u | g | G | A | G | A | u | G | G | C | A | C | A | G | A | C | G | G | a | 15 | 4 | 11 | bc | false | <90% | 5521 | ||
NM_007085 | FSTL1 | 1007 | 1025 | A | U | G | A | G | g | A | G | G | C | A | C | A | G | A | C | c | a | G | 10 | 3 | 10 | c | false | <90% | 3705 | ||
XM_001127227 | LOC728331 | 678 | 696 | g | g | a | A | G | A | g | G | G | C | A | C | A | G | A | C | G | G | G | 0 | 4 | 12 | bc | false | <90% | 711 |
3 prime UTR Seed matches
Hits to same gene : 0
Hits to other genes : 1225
View matched accessions
3 prime seed match details
Immunostimulatory motif search results
UGUGU - Judge et al. Sequence-dependent stimulation of the mammalian innate immune response by synthetic siRNA. Nat Biotechnol. 2005 Apr;23(4):457-62. (PMID: 15778705)
No Immunostimulatory motif found (UGUGU).
GUCCUUCAA - Hornung et al. Sequence-specific potent induction of IFN-alpha by short interfering RNA in plasmacytoid dendritic cells through TLR7. Nat Med. 2005 Mar;11(3):263-70. Epub 2005 Feb 20. (PMID: 15723075)
No Immunostimulatory motif found (GUCCUUCAA).
Settings used for search
Scores were calculated using scoring scheme SS1
Using AVG scoring mode.
Hit scores
Parameter | Spec. Score cut-off | Colour | Comment |
Hit to same gene | Colour | Hits to the same accession or genes with 100 percent identity (i.e. likely transcript variants). It is advisable to always check the genes hit. | |
High scoring hit | ≥80 | Colour | Hits are very likely to cause an off-target effect |
Medium scoring hit | <80¸≥40 | Colour | Hits with a small chance of causing an off target effect. |
Low scoring hit | Colour | Hits very unlikely to cause an off-target effect |
The mismatches are colour coded based on the weighting of that position.
Cut-off | Colour |
Match | Colour |
Wobble base pairs (G-U) | Colour |
Critical bases (none) | Colour |
0.6 bits | Colour |
0.4 bits | Colour |
0.2 bits | Colour |
SpecificityServer is designed to help you identify potential non-specific matches to your siRNA. It incorporates the latest information about non-specific matches (sequence-specific only).
Designed by: Alistair Chalk, Quan Du, Zicai Liang and Erik L.L. Sonnhammer