Automatic recommendation
OK - siRNA is specific for both sense and antisense (guide) strands
14 hits were found for UCCUCACAAUACCGCAGAG (11 guide strand hits and 3 sense strand hits.)
Hits to Guide strand
| Accession | Gene | Start | End | Details of mismatches | Spec. Score | Mismatches | Contiguous | Jackson style specificity | Hits similar gene | HSP Score | HSP % Identity | HSP length | Gene Length | ||||||||||||||||||
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | |||||||||||||
| NM_006955 | ZNF33B | 1731 | 1749 | a | C | C | U | C | A | C | A | A | U | A | C | a | u | C | A | G | A | G | 68 | 3 | 11 | false | <90% | 5958 | |||
| XM_942427 | C1orf34 | 531 | 549 | U | C | C | U | C | A | C | A | A | U | A | C | u | G | C | A | a | g | G | 63 | 3 | 12 | false | <90% | 2801 | |||
| XM_930328 | C1orf34 | 531 | 549 | U | C | C | U | C | A | C | A | A | U | A | C | u | G | C | A | a | g | G | 63 | 3 | 12 | false | <90% | 2801 | |||
| XM_945965 | C1orf34 | 598 | 616 | U | C | C | U | C | A | C | A | A | U | A | C | u | G | C | A | a | g | G | 63 | 3 | 12 | false | <90% | 2868 | |||
| XM_934658 | C1orf34 | 598 | 616 | U | C | C | U | C | A | C | A | A | U | A | C | u | G | C | A | a | g | G | 63 | 3 | 12 | false | <90% | 2868 | |||
| XM_945959 | C1orf34 | 619 | 637 | U | C | C | U | C | A | C | A | A | U | A | C | u | G | C | A | a | g | G | 63 | 3 | 12 | false | <90% | 2889 | |||
| NM_001080494 | C1orf34 | 619 | 637 | U | C | C | U | C | A | C | A | A | U | A | C | u | G | C | A | a | g | G | 63 | 3 | 12 | false | <90% | 2889 | |||
| XM_945958 | C1orf34 | 749 | 767 | U | C | C | U | C | A | C | A | A | U | A | C | u | G | C | A | a | g | G | 63 | 3 | 12 | false | <90% | 6268 | |||
| XM_934649 | C1orf34 | 749 | 767 | U | C | C | U | C | A | C | A | A | U | A | C | u | G | C | A | a | g | G | 63 | 3 | 12 | false | <90% | 6268 | |||
| NM_016448 | DTL | 663 | 681 | a | g | C | U | C | A | C | A | A | U | A | C | C | u | C | A | G | A | c | 62 | 4 | 11 | c | false | <90% | 4221 | ||
| NM_152615 | PARP15 | 1247 | 1265 | U | C | C | U | C | A | C | A | A | U | c | C | C | a | C | A | G | A | u | 14 | 3 | 10 | c | false | <90% | 1956 | ||
Hits to Sense strand
| Accession | Gene | Start | End | Details of mismatches | Spec. Score | Mismatches | Contiguous | Jackson style specificity | Hits similar gene | HSP Score | HSP % Identity | HSP length | Gene Length | ||||||||||||||||||
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | |||||||||||||
| XM_943773 | LOC283143 | 2186 | 2204 | C | U | C | a | G | a | G | G | U | A | U | U | G | U | G | A | G | G | u | -5 | 3 | 12 | bc | false | <90% | 3322 | ||
| XM_378312 | LOC283143 | 2186 | 2204 | C | U | C | a | G | a | G | G | U | A | U | U | G | U | G | A | G | G | u | -5 | 3 | 12 | bc | false | <90% | 3322 | ||
| NM_001004342 | TRIM67 | 7013 | 7031 | C | U | C | U | G | a | u | G | U | A | U | U | G | U | G | A | G | G | A | -29 | 2 | 12 | bc | false | <90% | 8522 | ||
3 prime UTR Seed matches
Hits to same gene : 0
Hits to other genes : 2437
View matched accessions
3 prime seed match details
Immunostimulatory motif search results
UGUGU - Judge et al. Sequence-dependent stimulation of the mammalian innate immune response by synthetic siRNA. Nat Biotechnol. 2005 Apr;23(4):457-62. (PMID: 15778705)
No Immunostimulatory motif found (UGUGU).
GUCCUUCAA - Hornung et al. Sequence-specific potent induction of IFN-alpha by short interfering RNA in plasmacytoid dendritic cells through TLR7. Nat Med. 2005 Mar;11(3):263-70. Epub 2005 Feb 20. (PMID: 15723075)
No Immunostimulatory motif found (GUCCUUCAA).
Settings used for search
Scores were calculated using scoring scheme SS1
Using AVG scoring mode.
Hit scores
| Parameter | Spec. Score cut-off | Colour | Comment |
| Hit to same gene | Colour | Hits to the same accession or genes with 100 percent identity (i.e. likely transcript variants). It is advisable to always check the genes hit. | |
| High scoring hit | ≥80 | Colour | Hits are very likely to cause an off-target effect |
| Medium scoring hit | <80¸≥40 | Colour | Hits with a small chance of causing an off target effect. |
| Low scoring hit | Colour | Hits very unlikely to cause an off-target effect |
The mismatches are colour coded based on the weighting of that position.
| Cut-off | Colour |
| Match | Colour |
| Wobble base pairs (G-U) | Colour |
| Critical bases (none) | Colour |
| 0.6 bits | Colour |
| 0.4 bits | Colour |
| 0.2 bits | Colour |
SpecificityServer is designed to help you identify potential non-specific matches to your siRNA. It incorporates the latest information about non-specific matches (sequence-specific only).
Designed by: Alistair Chalk, Quan Du, Zicai Liang and Erik L.L. Sonnhammer