Predicted Off-targets for siRNA "gugucaaggaggaucgacu" Using siRNA SpecificityServer

Specificity Server [Result page v1.0]


SpecificityServer - An siRNA design program for determining siRNA specificity

Automatic recommendation
OK - siRNA is specific for both sense and antisense (guide) strands

10 hits were found for GUGUCAAGGAGGAUCGACU (4 guide strand hits and 6 sense strand hits.)

Hits to Guide strand

AccessionGeneStartEndDetails of mismatchesSpec.
Score
MismatchesContiguousJackson style
specificity
Hits similar
gene
HSP
Score
HSP %
Identity
HSP lengthGene Length
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19
NM_007050 PTPRT 2064 2082 uUGUCAAGGAGGAgCGACU78 2 12 c false   <90%   12653
NM_133170 PTPRT 2064 2082 uUGUCAAGGAGGAgCGACU78 2 12 c false   <90%   12710
NM_174933 PHYHD1 990 1008 GUGUCAAGGAGGAUgGuCc46 3 14 c false   <90%   1616
NM_002386 MC1R 1702 1720 uUGUCAAaGAGGAUgGACU11 3 6 c false   <90%   2400


Hits to Sense strand







AccessionGeneStartEndDetails of mismatchesSpec.
Score
MismatchesContiguousJackson style
specificity
Hits similar
gene
HSP
Score
HSP %
Identity
HSP lengthGene Length
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19
NM_001037165 FOXK1 7201 7219 ccUCGAgCCUCCUUGACAa15 4 11 bc false   <90%   11191
NM_057167 COL6A3 7034 7052 AGUCGgUCCUCCUgGACAa12 3 7 c false   <90%   9944
NM_057166 COL6A3 7049 7067 AGUCGgUCCUCCUgGACAa12 3 7 c false   <90%   9959
NM_057165 COL6A3 7052 7070 AGUCGgUCCUCCUgGACAa12 3 7 c false   <90%   9962
NM_057164 COL6A3 7151 7169 AGUCGgUCCUCCUgGACAa12 3 7 c false   <90%   10061
NM_004369 COL6A3 7652 7670 AGUCGgUCCUCCUgGACAa12 3 7 c false   <90%   10562


3 prime UTR Seed matches

Hits to same gene : 0
Hits to other genes : 1277
View matched accessions

3 prime seed match details

AccessionGene nameHits to this gene
NM_024577SH3TC24
NM_016206VGLL33
NM_0209103
NM_001080437SNED13
XM_940984SNED13
NM_018375SLC39A92
NM_014758SNX192
NM_173851SLC30A82
NM_145206VTI1A2
NM_182831TNT2
NM_020796SEMA6A2
NM_021961TEAD12
NM_134431SLCO1A22
NM_016248AKAP112
NM_001025233CLLU12
NM_014423AFF42
NM_001024460VNN32
NM_078625VNN32
NM_018399VNN32
NM_000046ARSB2
NM_014850SRGAP32
NM_001033117SRGAP32
NM_0010992722
NM_152733BTBD92
NM_0528932
NM_181709FAM101A2
NM_207469DEFB322
NM_001106ACVR2B2
NM_203365RAPH12
NM_022080NAPB2
NM_024345WDR322
NM_138806CD200R12
NM_170780CD200R12
XM_001131702LOC1527422
XM_001128848LOC1527422
NM_002751MAPK112
NM_031912SYT152
NM_004663RAB11A2
NM_005296GPR232
XM_379694LOC4015772
XM_942510LOC6528272
XM_928150LOC4015772
NM_020338ZMIZ12
NM_033637BTRC2
NM_003939BTRC2
NM_015271TRIM22
NM_033121ANKRD13A2
XM_001132564LOC7288822
XM_001133521MGC513382
NM_001024372BAALC2
NM_018566YOD12
NM_017811UBE2R22
NM_015852ZNF1172
XM_001131007LOC7296782
XM_001127177LOC7307802
NM_194429FGFR1OP2
NM_007045FGFR1OP2
NM_021643TRIB22
NM_018361AGPAT52
NM_014730KIAA01522
NM_006141DYNC1LI22
NM_001042383CEP632
NM_001042400CEP632
NM_025180CEP632
NM_016052RRP152
NM_001018067SERBP12
NM_015640SERBP12
NM_001018069SERBP12
NM_001018068SERBP12
NM_017712PGPEP12
NM_018941CLN82
NM_007011ABHD22
NM_152924ABHD22
NM_0010992942
XM_936510KIAA16442
NM_020133AGPAT42
XM_938374LOC6440102
NM_016089ZNF5892
NM_001007536AKR1CL12
NM_020297ABCC92
NM_021632ZNF3502
NM_020804PACSIN11
NM_0011004081
NM_001033046C17orf621
NM_0011004071
NM_152705POLR1D1
NM_001083113ZGPAT1
NM_181485ZGPAT1
NM_032527ZGPAT1
NM_018670MESP11
NM_003115UAP11
NM_005098MSC1
NM_001455FOXO3A1
NM_201559FOXO3A1
NM_145290GPR1251
NM_022103ZNF6671
NM_173829P18SRP1
NM_001375DNASE21
NM_173465COL23A11
NM_0011006241
NM_001038628B3GALNT11
NM_033169B3GALNT11
NM_033168B3GALNT11
NM_003781B3GALNT11
NM_033167B3GALNT11
NM_198219ING11
NM_198218ING11
NM_005537ING11
NM_198217ING11
NM_007110TEP11
NM_173812DPY19L21
NM_001011885BTBD11
NM_025238BTBD11
NM_207317ZNF4741
NM_001001891TMEM16G1
XM_001130807LOC7317871
NM_001008895CUL4A1
NM_003589CUL4A1
NM_032810ATAD11
NM_015092SMG11
NM_006620HBS1L1
NM_001080484KIAA17511
XM_936307KIAA17511
NM_033426KIAA17371
NM_005756GPR641
NM_001079859GPR641
NM_001079858GPR641
NM_001079860GPR641
NM_003848SUCLG21
NM_003575ZNF2821
NM_153634CPNE81
NM_005342HMGB31
NM_024921POF1B1
NM_016282AK31
NM_001082575HRNBP31
XM_001129103LOC7290291
XM_001128912LOC7312381
NM_032195SON1
NM_0154731
XM_378914KIAA04921
XM_944290KIAA04921
NM_014951ZNF3651
XM_001131004LOC7318401
XM_001131944LOC7299801
NM_032148SLC41A21
NM_138401FAM125A1
NM_032021TMEM1331
NM_020803KLHL81
NM_032682FOXP11
NM_005347HSPA51
NM_052859RFT11
NM_014877HELZ1
NM_173635FLJ402351
NM_018660ZNF3951
NM_013260SAP30BP1
NM_133367PAQR81
NM_018327SPTLC31
NM_005126NR1D21
XM_001130839NR1D21
NM_001812CENPC11
NM_024092TMEM1091
NM_003423ZNF431
NM_052885SLC2A131
NM_032505KBTBD81
NM_006885ATBF11
NM_006675TSPAN91
NM_206933USH2A1
NM_004707ATG121
NM_201284EGFR1
NM_153456HS6ST31
NM_001080975REPS21
NM_004726REPS21
NM_012425RSU11
NM_152724RSU11
XM_001130021LOC7312921
NM_000314PTEN1
NM_005933MLL1
NM_002735PRKAR1B1
NM_017780CHD71
NM_025054VCPIP11
NM_0011051931
NM_0011051951
NM_0011051941
NM_152698FLJ383771
NM_021721ADAM221
NM_004194ADAM221
NM_021960MCL11
NM_182763MCL11
NM_002053GBP11
NM_031483ITCH1
NM_004423DVL31
NM_000362TIMP31
NM_178425HDAC91
NM_178423HDAC91
NM_001040101D4S234E1
NM_014392D4S234E1
NM_032389ZNF2891
NM_002841PTPRG1
NM_182503DEADC11
NM_021738SVIL1
NM_003174SVIL1
NM_138379TIMD41
NM_018092NETO21
XM_934889FHAD11
XM_946265FHAD11
NM_194291TMEM651
NM_001040000MLLT41
NM_012471TRPC51
NM_002577PAK21
NM_144647CAPSL1
NM_001042625CAPSL1
NM_0149801
NM_016045SLMO21
NM_002576PAK11
NM_005759ABI21
XM_001126750ABI21
NM_001126ADSS1
NM_001946DUSP61
NM_022652DUSP61
NM_004296RGS61
NM_018844BCAP291
NM_182765HECTD21
NM_182756SPDYA1
NM_005160ADRBK21
NM_032578MYPN1
NM_001077708ANKRD71
NM_019644ANKRD71
NM_000282PCCA1
NM_014746RNF1441
NM_017740ZDHHC71
NM_003715VDP1
NM_033331CDC14B1
NM_003671CDC14B1
NM_001077181CDC14B1
NM_003292TPR1
NM_030641APOL61
NM_145272C17orf501
NM_002787PSMA21
NM_152696HIPK11
NM_057178RFFL1
NM_001017368RFFL1
NM_004120GBP21
NM_033150COL2A11
NM_001844COL2A11
NM_030964SPRY41
NM_014817KIAA06441
NM_001031855LONRF31
NM_024778LONRF31
NM_001001669FLJ416031
NM_000242MBL21
NM_052918SORCS11
NM_001013031SORCS11
NM_000587C71
NM_033313CDC14A1
NM_001014380KATNAL11
NM_032116KATNAL11
NM_004431EPHA21
NM_032047B3GNT51
NM_001004686OR2L21
XM_941731Klkbl41
NM_001080492Klkbl41
NM_020448NPAL31
NM_020754CDGAP1
XM_942780SYNPO21
NM_002227JAK11
NM_0207251
NM_002866RAB3A1
NM_007350PHLDA11
XM_001132421LOC3883121
XM_373814LOC3885721
XM_001125791LOC6427881
XM_001127886LOC7281001
XM_001128749LOC7280541
XM_001133085LOC7287901
NM_014717ZNF5361
NM_001083620GRIA21
NM_000826GRIA21
NM_001083619GRIA21
NM_020116FSTL51
NM_024831TGS11
NM_018196TMLHE1
NM_015275KIAA10331
NM_022831C1orf801
XM_930476LOC6536311
XM_001134276C1orf801
NM_001004441DP581
NM_018290PGM21
NM_003412ZIC11
NM_178313SPTBN11
NM_032594INSM21
XM_373553LOC3878951
XM_945559LOC3878951
NM_0529271
NM_178177NMNAT31
NM_173607C14orf241
NM_001079519C14orf241
NM_006352ZNF2381
NM_205768ZNF2381
NM_021938BRUNOL51
NM_012279ZNF3461
NM_005603ATP8B11
NM_006482DYRK21
NM_003583DYRK21
NM_018394ABHD101
NM_138330ZNF6751
NM_004796NRXN31
NM_0011052501
NM_138970NRXN31
NM_030660ATXN31
NM_004993ATXN31
NM_018295TMEM1401
NM_207307C3orf251
NM_020935USP371
NM_153341IBRDC31
NM_138375CABLES11
NM_145307PLEKHK11
NM_178565RSPO21
NM_152418WDR21C1
NM_024574C4orf311
NM_001609ACADSB1
NM_001039585PTGFR1
NM_000959PTGFR1
NM_002240KCNJ61
NM_002215ITIH11
NM_017628KIAA15461
NM_001039770FLJ450321
NM_080632UPF3B1
NM_023010UPF3B1
NM_194314ZBTB411
NM_207333ZNF3201
NM_030802FAM117A1
NM_015488PNKD1
NM_022572PNKD1
NM_152734C6orf891
NM_139126PPIL41
NM_024783AGBL21
NM_033468ZNF2571
XM_928029RNF1871
XM_001129309RNF1871
NM_007249KLF121
NM_173214NFAT51
NM_006599NFAT51
NM_138714NFAT51
NM_0011131781
NM_138713NFAT51
NM_007220CA5B1
NM_017919STX171
NM_015568PPP1R16B1
NM_024110CARD141
NM_032213RBED11
NM_017849TMEM1271
NM_001042574CRTC31
NM_022769CRTC31
NM_012335MYO1F1
XM_933796SLC45A41
NM_001024647RAB3IP1
NM_022456RAB3IP1
NM_175624RAB3IP1
NM_175625RAB3IP1
NM_175623RAB3IP1
NM_018036C14orf1031
NM_006038SPATA21
XM_001132925DUSP221
NM_004273CHST31
NM_015520MAGI11
NM_001033057MAGI11
NM_012214MGAT4A1
NM_000459TEK1
NM_015995KLF131
NM_001033571AMZ21
NM_001033574AMZ21
NM_001033572AMZ21
NM_001033569AMZ21
NM_001033570AMZ21
NM_016627AMZ21
NM_016255FAM8A11
NM_153607LOC1532221
NM_001017979RAB281
XM_001127597FLJ328101
XM_001127587FLJ328101
XM_925815FLJ328101
XM_001127637FLJ328101
XM_001127623FLJ328101
NM_030817APOLD11
NM_020463SMEK21
XM_496597FLJ304281
NM_0010988331
NM_0202181
NM_057749CCNE21
NM_021645UTP14C1
NM_207405FLJ464811
NM_015332NUDCD31
NM_134266SLC26A71
NM_030939C6orf621
NM_015185ARHGEF91
NM_033551LARP11
NM_015315LARP11
NM_025040ZNF6141
NM_144665SESN31
NM_002253KDR1
NM_003126SPTA11
NM_001007024GOSR11
NM_001007025GOSR11
NM_004871GOSR11
NM_001031666MS4A31
NM_001031809MS4A31
NM_006138MS4A31
XM_926528LOC4399921
XM_943171LOC4399921
XM_495839LOC4399921
XM_937069LOC4399921
NM_001018005TPM11
NM_000366TPM11
NM_014248RBX11
NM_001039199C20orf1211
NM_024331C20orf1211
NM_001009959KIAA11891
NM_020711KIAA11891
NM_004703RABEP11
NM_001083585RABEP11
NM_033274ADAM191
NM_0011004251
NM_015314KIAA08951
NM_016072GOLT1B1
NM_152417TMEM681
XM_931945LOC6439621
XM_943665LOC6439621
NM_003099SNX11
NM_152826SNX11
NM_148955SNX11
NM_012247SEPHS11
NM_005596NFIB1
NM_020151STARD71
NM_199053FLJ127161
NM_021942FLJ127161
NM_017643MBTD11
NM_021999ITM2B1
NM_018409LRP2BP1
XM_943032LOC6478051
NM_001014812FAM96A1
NM_032231FAM96A1
NM_004975KCNB11
NM_001031723DNAJB141
NM_024920DNAJB141
NM_004783TAOK21
NM_005938MLLT71
NM_001037131CENTG21
NM_014914CENTG21
NM_005927MFAP31
XM_045290LOC1515791
XM_938633LOC1515791
NM_017640LRRC161
NM_001012981ZKSCAN21
NM_021269ZNF7081
NM_014015DEXI1
NM_003836DLK11
NM_182976ZNF3261
NM_181782NCOA71
NM_203382AMACR1
NM_003637ITGA101
XM_001132495SLC26A111
NM_173626SLC26A111
NM_174929ZMIZ21
NM_031449ZMIZ21
NM_148674SMC1B1
NM_015323KIAA07761
NM_012204GTF3C41
NM_001039845MDH1B1
NM_022748TNS31
NM_207660ZC3H141
NM_024824ZC3H141
NM_207661ZC3H141
NM_207662ZC3H141
NM_024843CYBRD11
NM_003505FZD11
NM_024610HSPBAP11
NM_014313TMEM50A1
NM_022150NPVF1
NM_014803ZNF5181
NM_001025780FAM108B11
NM_031279AGXT2L11
NM_020947KIAA16091
NM_005590MRE11A1
NM_005591MRE11A1
NM_176814ZNF8001
NM_004362CLGN1
NM_001076786QSER11
NM_173602DIP2B1
NM_207037TCF121
NM_003205TCF121
NM_207040TCF121
NM_207036TCF121
NM_207038TCF121
NM_022840METTL41
NM_018100EFHC11
NM_015359SLC39A141
NM_001012751ABI11
NM_001012750ABI11
NM_001012752ABI11
NM_005470ABI11
NM_005786TSHZ11
NM_001007525NWD11
NM_152280SYT111
NM_021927GUF11
NM_025147COQ10B1
NM_021083XK1
NM_002851PTPRZ11
NM_002480PPP1R12A1
NM_139289AKAP41
NM_003886AKAP41
NM_032735BEST31
NM_152439BEST31
NM_014743KIAA02321
NM_0011005901
NM_001002860BTBD71
NM_0247761
NM_025074FRAS11
NM_006581FUT91
NM_005036PPARA1
NM_001001928PPARA1
XM_938194LOC1276021
XM_059166LOC1276021
NM_006918SC5DL1
NM_001024956SC5DL1
NM_173825RABL31
XM_926634LOC6532561
NM_014862ARNT21
NM_0010988321
NM_032837FAM104A1
NM_133493CD1091
NM_017592IXL1
NM_003200TCF31
NM_032551KISS1R1
NM_153256C10orf471
NM_178544ZNF5461
NM_018939PCDHB61
NM_015294TRIM371
NM_001042646TRAK11
NM_004473FOXE11
NM_006226PLCL11
NM_080669SLC46A11
NM_152375KLHDC7A1
NM_020818KIAA14091
NM_014309RBM91
NM_001082576RBM91
NM_001082577RBM91
NM_001031695RBM91
NM_001082578RBM91
NM_001082579RBM91
XM_938090PEAR11
NM_001080471PEAR11
NM_002340LSS1
NM_017420SIX41
XM_001131823ANKRD30A1
NM_052997ANKRD30A1
NM_021202TP53INP21
NM_001012961C1orf841
NM_032359C3orf261
NM_033224PURB1
NM_002747MAPK41
NM_007106UBL31
NM_003307TRPM21
NM_001042493C6orf1621
NM_020425C6orf1621
NM_014424HSPB71
NM_002098GUCA1B1
NM_021007SCN2A1
NM_001040142SCN2A1
NM_001040143SCN2A1
NM_014044UNC501
NM_016532SKIP1
NM_130766SKIP1
NM_170724PKHD11
NM_032458PHF61
NM_001015877PHF61
NM_017623CNNM31
NM_199078CNNM31
NM_019843EIF4ENIF11
NM_022346NCAPG1
NM_000161GCH11
XM_001130189LOC7315981
XM_001129850LOC7292911
NM_001905CTPS1
NM_001560IL13RA11
NM_005096ZMYM31
NM_201599ZMYM31
NM_016084RASD11
NM_032778MINA1
NM_153182MINA1
NM_001042533MINA1
NM_018958C15orf21
NM_001001931MTUS11
NM_001001924MTUS11
NM_020749MTUS11
NM_001001925MTUS11
NM_0150381
NM_013444UBQLN21
NM_139169TRUB11
NM_021140UTX1
NM_004852ONECUT21
NM_194286KIAA18531
NM_014679CEP571
NM_021136RTN11
NM_206852RTN11
NM_206857RTN11
NM_080737SYTL41
NM_005521TLX11
NM_000486AQP21
NM_019117KLHL41
NM_001044722CSNK1G31
NM_001031812CSNK1G31
NM_001044723CSNK1G31
NM_004384CSNK1G31
NM_032873STS-11
NM_022458LMBR11
NM_017944USP471
XM_373690LOC3882821
XM_001133719LOC3882821
NM_018479ECHDC11
NM_0010020301
NM_0011055441
NM_0011055451
NM_031445AMMECR1L1
NM_001816CEACAM81
NM_018708FEM1A1
NM_003870IQGAP11
NM_030639BHLHB91
NM_005082TRIM251
NM_004285H6PD1
NM_015044GGA21
NM_020404CD2481
NM_015157PHLDB11
NM_030780SLC25A321
NM_001512GSTA41
NM_016169SUFU1
NM_001040633PRKAG21
NM_016203PRKAG21
NM_024429PRKAG21
NM_006372SYNCRIP1
NM_001008393LOC2017251
NM_001006114C21orf571
NM_001010971SAMD131
NM_1528331
XM_946032LOC6448521
XM_934218LOC6448521
NM_014911AAK11
XM_928637LOC6431591
XM_942664LOC6431591
NM_052831C6orf1921
NM_020661AICDA1
NM_018128TSR11
NM_002820PTHLH1
NM_198964PTHLH1
NM_0010987901
NM_021242MID1IP11
NM_0010987911
NM_033238PML1
NM_144633KCNH81
NM_145291ZNF5091
NM_020698TMCC31
NM_0010839621
NM_003199TCF41
NM_016478ZC3HC11
NM_002711PPP1R3A1
NM_005628SLC1A51
NM_178011LRRTM31
NM_014096SLC43A31
NM_199329SLC43A31
NM_017611SLC43A31
XM_942240LOC6505571
NM_033058TRIM551
NM_184085TRIM551
NM_184086TRIM551
NM_184087TRIM551
NM_173578C11orf721
NM_001002862DERL31
NM_198440DERL31
NM_014760TATDN21
NM_000674ADORA11
NM_001048230ADORA11
NM_133448TMEM132D1
NM_145861EDARADD1
NM_080738EDARADD1
XM_001125753MUC3B1
NM_006955ZNF33B1
NM_007271STK381
NM_002649PIK3CG1
NM_016096ZNF7061
NM_001042511ZNF7061
NM_001042510ZNF7061
NM_014333CADM11
NM_0010985171
NM_006315PCGF31
NM_002031FRK1
NM_207647FSD1L1
NM_000945PPP3R11
NM_001080530LOC920171
NM_006922SCN3A1
NM_001081677SCN3A1
NM_001081676SCN3A1
NM_032818C9orf1001
NM_130847AMOTL11
NM_020774MIB11
NM_014225PPP2R1A1
NM_002868RAB5B1
NM_024626VTCN11
NM_012262HS2ST11
NM_018402IL261
NM_014480ZNF5441
XM_001126406LOC7279221
XM_001126391LOC7279221
XM_001126417LOC7279221
NM_022173TIA11
NM_022037TIA11
XM_371369MIA31
XM_943876MIA31
XM_943869MIA31
XM_943873MIA31
XM_496436MIA31
XM_932541MIA31
XM_932548MIA31
NM_198551MIA31
NM_001030005CPLX31
NM_207380FLJ433391
NM_012406PRDM41
NM_001039709CBFA2T21
NM_001032999CBFA2T21
NM_005093CBFA2T21
NM_007007CPSF61
NM_181900STARD51
NM_016281TAOK31
NM_015576ERC21
NM_020726NLN1
NM_0207221
NM_001278CHUK1
NM_144571CNOT6L1
NM_022783DEPDC61
NM_004397DDX61
NM_000431MVK1
NM_175861TMTC11
NM_014372RNF111
NM_153645NUP501
NM_007172NUP501
NM_0152761
NM_015375RIPK51
NM_199462RIPK51
NM_003774GALNT41
NM_020199C5orf151
NM_022459XPO41
NM_001081573GAB31
NM_080612GAB31
XM_001132528NPTX11
NM_002522NPTX11
NM_052926PNMA51
NM_0011031511
NM_0011031501
NM_014141CNTNAP21
NM_001105ACVR11
NM_134428RFX31
NM_016540GPR831
NM_002718PPP2R3A1
NM_181897PPP2R3A1
NM_014181HSPC1591
NM_0146921
NM_015879ST8SIA31
NM_005504BCAT11
NM_002271RANBP51
NM_001031677RAB241
NM_130781RAB241
NM_001001551C9orf1031
NM_001037165FOXK11
NM_020801ARRDC31
NM_001945HBEGF1
NM_004572PKP21
NM_001005242PKP21
NM_018984SSH11
NM_015462NOL111
NM_138788TMEM45B1
NM_020381PDSS21
NM_153023SPATA131
NM_001077401ACVRL11
NM_000020ACVRL11
NM_080820DTD11
NM_138783ZNF6531
NM_018129PNPO1
NM_020799STAMBPL11
NM_0153021
NM_007256SLCO2B11
NM_001010974BCAS41
NM_198799BCAS41
NM_017843BCAS41
NM_018405C17orf791
XM_376305DUB31
NM_016538SIRT71
NM_001040285PAPD51
NM_001040284PAPD51
NM_022896LPIN31
NM_001007527LMBRD21
NM_017779DEPDC11
NM_030637DDHD11
NM_0221591
NM_182943PLOD21
NM_000935PLOD21
NM_002357MXD11
NM_152588TMTC21
NM_001077523AP3D11
NM_003938AP3D11
NM_030815PDRG11
XM_001129441LOC6451661
XM_946374LSP11
NM_138621BCL2L111
NM_006538BCL2L111
NM_001081461JMJD61
NM_004232SOCS61
NM_006996SLC19A21
NM_004028AQP41
NM_001650AQP41
NM_012483GNLY1
NM_006433GNLY1
NM_019083CCDC761
NM_004569PIGH1
NM_020888KIAA15221
NM_145048C4orf281
NM_000963PTGS21
NM_001077503TAC41
NM_001077505TAC41
NM_170685TAC41
NM_001077506TAC41
NM_001077504TAC41
NM_173663NY-REN-71
NM_001079527LOC6533161
NM_198581ZC3H61
NM_152758YTHDF31
NM_001042631LOC6440961
NM_003217TEGT1
NM_0010985761
NM_002885RAP1GAP1
NM_015261NCAPD31
NM_007355HSP90AB11
NM_207577MAP61
NM_017409HOXC101
NM_021977SLC22A31
XM_945995LOC3396741
XM_934917LOC3396741
NM_002978SCNN1D1
NM_005801EIF11
NM_018706DHTKD11
XM_936868C10orf1361
XM_926383C10orf1361
NM_144991C21orf291
NM_013363PCOLCE21
NM_000845GRM81
NM_001969EIF51
NM_183004EIF51
NM_152265BTF3L41
NM_198859PRICKLE21
NM_145259ACVR1C1
NM_0011110311
NM_0011110331
NM_0011110321
NM_1735691
NM_001007246BRWD11
NM_003782B3GALT41
NM_015149RGL11
NM_003681PDXK1
NM_006850IL241
NM_181339IL241
NM_021111RECK1
NM_001789CDC25A1
NM_201567CDC25A1
NM_170731BDNF1
NM_001709BDNF1
NM_170732BDNF1
NM_170733BDNF1
NM_170734BDNF1
NM_170735BDNF1
NM_001039547GK51
NM_024896KIAA18151
NM_004745DLGAP21
XM_941762SETD1B1
XM_037523SETD1B1
NM_020699GATAD2B1
NM_080760DACH11
NM_080759DACH11
NM_004392DACH11
NM_023080C8orf331
NM_018085IPO91
NM_0011025661
NM_006536CLCA21
XM_934649C1orf341
XM_945958C1orf341
NM_020309SLC17A71
NM_004744LRAT1
NM_006736DNAJB21
XM_380095LOC4013171
XM_379479LOC4013171
XM_938320LOC4013171
NM_176823S100A7A1
NM_152703SAMD9L1
NM_015116LRCH11
NM_001010980C1orf1301
NM_016150ASB21
NM_002917RFNG1
XM_001132711RFNG1
NM_017551GRID11
NM_017902HIF1AN1
NM_001004342TRIM671
NM_198269HIPK11
NM_181358HIPK11
NM_198268HIPK11
XM_001128879LOC7289031
XM_001128866LOC7289031
NM_014819PJA21
NM_015225KIAA03671
NM_013245VPS4A1
NM_024909C6orf1341
NM_017671C20orf421
NM_012448STAT5B1
NM_015367BCL2L131
NM_207514FLJ201861
NM_033221TRIM141
NM_014788TRIM141
NM_001008537KIAA20221
NM_001039SCNN1G1
NM_020120UGCGL11
NM_001025777UGCGL11
NM_018593SLC16A101
NM_0010994551
NM_018340FLJ111511
NM_002646PIK3C2B1
NM_014314DDX581
NM_152834TMEM181
NM_148920PIGQ1
NM_004204PIGQ1
NM_198968DZIP11
NM_014934DZIP11
NM_018106ZDHHC41
NM_007232HRH31
NM_001010892RSHL31
XM_001128598LOC7286731
NM_014636RALGPS11
NM_198963DHX571
NM_001006657WDR351
NM_020779WDR351
NM_1522831
NM_030786SYNC11
NM_018325C9orf721
NM_004093EFNB21
NM_013236ATXN101
NM_002881RALB1
NM_030949PPP1R14C1
NM_181655C17orf581
NM_181656C17orf581
NM_145039MGC163851
NM_017759FLJ203091
NM_024701ASB131
NM_003889NR1I21
NM_022002NR1I21
NM_033013NR1I21
NM_007157ZXDB1
NM_006251PRKAA11
NM_206907PRKAA11
NM_001080421UNC13A1
XM_934118LOC6534891
XM_934115LOC6534891
NM_032295SLC37A31
NM_207113SLC37A31
NM_005471GNPDA11
NM_152686DNAJC181
NM_173078SLITRK41
XM_171855LOC2552751
XM_941352LOC2552751
NM_025201PLEKHQ11
NM_000629IFNAR11
NM_016009SH3GLB11
NM_005868BET11
NM_004447EPS81
NM_139279MCFD21
NM_001013649LOC3889691
NM_006154NEDD41
NM_198400NEDD41
NM_002737PRKCA1
NM_018951HOXA101
NM_153715HOXA101
NM_139283PPTC71
NM_031899GORASP11
NM_005171ATF11
NM_152275TTC30A1
NM_175921LOC2856361
NM_001551IGBP11
NM_015453THUMPD31
NM_016569TBX31
NM_005996TBX31
NM_004992MECP21
NM_001003676C11orf491
NM_032108SEMA6B1
NM_016059PPIL11
NM_206917NGFRAP11
NM_206915NGFRAP11
NM_014380NGFRAP11
NM_021248CDH221
NM_0010854001
NM_003954MAP3K141
NM_002170IFNA81
NM_001079673FNDC3A1
NM_014923FNDC3A1
NM_020921NIN1
NM_145693LPIN11
NM_001048201UHRF11
NM_013282UHRF11
NM_002715PPP2CA1
NM_032509RBM131
NM_173509C1orf761
NM_030667PTPRO1
NM_002848PTPRO1
NM_030668PTPRO1
NM_030669PTPRO1
NM_030671PTPRO1
NM_030670PTPRO1
NM_007030TPPP1
NM_001011645AR1
NM_000044AR1
NM_178868CMTM81
NM_001766CD1D1
NM_018480TMEM126B1
NM_006373VAT11
NM_002833PTPN91
XM_001128975PTPN91
XM_377761LOC4021001
XM_001129364LOC6452311
XM_928270LOC6452311
NM_006303JTV11
NM_013309SLC30A41
NM_018293ZNF6541
XM_001126706ZNF6541
NM_014634PPM1F1
NM_001005373LRSAM11
NM_138361LRSAM11
NM_001005374LRSAM11
NM_198596SULF21
NM_018837SULF21
NM_001864COX7A11
NM_002537OAZ21
NM_032048EMILIN21
NM_014793LCMT21
NM_002178IGFBP61
NM_152356ZNF4911
NM_024812BAALC1
NM_012079DGAT11
XM_932949RGNEF1
NM_173667FLJ375431
NM_012338TSPAN121
NM_001039891ZNF6741
NM_0155661
NM_138694PKHD11
NM_006059LAMC31
NM_145060C18orf241
NM_001039535C18orf241
NM_021624HRH41
NM_001077484SLC38A11
NM_030674SLC38A11
NM_178123SESTD11
NM_003190TAPBP1
NM_181847AMIGO21
NM_016485VTA11
NM_014906PPM1E1
NM_018040GPATCH21
NM_012318LETM11
NM_031442TMEM471
NM_152751C10orf301
NM_001712CEACAM11
NM_001024912CEACAM11
NM_145250RNASE111
NM_000202IDS1
NM_006915RP21
NM_017799C14orf1011
NM_015506MMACHC1
NM_022767ISG20L11
NM_003471KCNAB11
NM_172159KCNAB11
NM_172160KCNAB11
NM_000081LYST1
NM_002312LIG41
NM_0010982681
NM_206937LIG41
NM_001655ARCN11
NM_033105DNAJC5B1
NM_005399PRKAB21
NM_001018075NR3C11
NM_001018077NR3C11
NM_001018076NR3C11
NM_001018074NR3C11
NM_001024094NR3C11
NM_000176NR3C11
NM_001020825NR3C11
NM_182703ANKDD1A1
NM_015460MYRIP1
NM_005668ST8SIA41
NM_002310LIFR1
NM_014802KIAA05281
NM_001008493ENAH1
NM_018212ENAH1
NM_020657ZNF3041
NM_0011004231
NM_0011004221
NM_0011004241
NM_015535LOC260101
NM_014933SEC31A1
NM_016211SEC31A1
NM_001077207SEC31A1
NM_001077208SEC31A1
NM_001077206SEC31A1
NM_014293NPTXR1
XM_001127175LOC7283201
NM_207404ZNF6621
NM_025113C13orf181
NM_001009899KIAA20181
XM_001133525LOC7315011
NM_138402LOC933491
NM_133379TTN1
NM_006174NPY5R1
NM_007175ERLIN21
NM_021067GINS11
NM_003189TAL11
NM_020240CDC42SE21
NM_001038702CDC42SE21
XM_001128865LOC7284071
NM_152307C14orf1721
NM_000088COL1A11
NM_199141CARM11
NM_020443NAV11
NM_173562KCTD201
XM_001126326AGRIN1
NM_198576AGRN1
NM_0150161
NM_032849C13orf331
NM_005795CALCRL1
XM_001129292MBOAT21
NM_138799MBOAT21
NM_003590CUL31
NM_014283C1orf91
NM_002843PTPRJ1
NM_152874FAS1
NM_152876FAS1
NM_152877FAS1
NM_152872FAS1
NM_152873FAS1
NM_152875FAS1
NM_015026MON21
NM_182597FLJ395751
NM_000527LDLR1
NM_004778GPR441
NM_005897IPP1
NM_001083924C1orf1161
NM_023938C1orf1161
NM_177948ARMCX31
NM_177947ARMCX31
NM_016607ARMCX31
NM_032102SFRS2B1
NM_001077440BCLAF11
NM_001077441BCLAF11
NM_014739BCLAF11
NM_015335THRAP21
NM_001557IL8RB1
NM_033496HK11
NM_000188HK11
NM_033498HK11
NM_033500HK11
NM_033497HK11
NM_020975RET1
NM_003221TFAP2B1
NM_138795ARL8A1
NM_006868RAB311
NM_014903NAV31
NM_000165GJA11
NM_015170SULF11
NM_005048PTHR21
NM_032689ZNF6071
NM_001918DBT1
NM_016025METTL91
NM_001077180METTL91
NM_013997TAC11
NM_013998TAC11
NM_003182TAC11
NM_013996TAC11
NM_144723ZMAT21
NM_014655SLC25A441
NM_006379SEMA3C1
NM_032833PPP1R15B1
NM_012223MYO1B1
NM_0011013721
NM_139281WDR361
NM_004854CHST101
NM_004543NEB1
NM_032427MAML21
NM_016083CNR11
NM_033181CNR11
XM_001130518LOC7284851
XM_001133894LOC7319641
NM_005822DSCR1L11
NM_006011ST8SIA21
NM_001537HSBP11
NM_014494TNRC6A1
NM_024090ELOVL61
NM_003717NPFF1
NM_005415SLC20A11
NM_021130PPIA1
NM_016315GULP11
NM_004363CEACAM51
XM_001128799MAP3K21
NM_002612PDK41
NM_152609C1orf711
NM_020865DHX361
NM_012193FZD41
NM_145257C1orf961
NM_194247HNRPA31
NM_0334031
NM_000926PGR1
NM_003743NCOA11
NM_147233NCOA11
NM_147223NCOA11
NM_0011055371
NM_005081ZNF1421
NM_052995CLRN11
NM_174878CLRN11
NM_001080467MYO5B1
XM_939100MYO5B1
XM_001132013ANKH1
NM_054027ANKH1
NM_001356DDX3X1
NM_133266SHANK21
NM_144600C16orf631
NM_152350C17orf451
NM_020474GALNT11
NM_001009993LOC1300741
NM_014738KIAA01951
NM_000582SPP11
NM_001040058SPP11
NM_001040060SPP11
NM_001035513SDHC1
NM_001035512SDHC1
NM_003001SDHC1
NM_001035511SDHC1
NM_175887PRR151
NM_016519AMBN1
NM_014048MKL21
NM_020342SLC39A101
NM_000092COL4A41
XM_001128367LOC4399491
XM_001129241LOC4399491
NM_018326GIMAP41
XM_001125792LOC7278341
XM_926013LOC6423981
NM_175080P2RX51
NM_002561P2RX51
NM_153686LCORL1
NM_004612TGFBR11
NM_002863PYGL1
NM_012141INTS61
NM_001039937INTS61
NM_0011005951
NM_182620FAM33A1
NM_019073SPATA61
NM_153377LRIG31
NM_000084CLCN51
NM_018263ASXL21
NM_032900ARHGAP191
XM_001128468NSF1
NM_006178NSF1
NM_006544EXOC51
NM_198715PTGER31
NM_001007088ZNF1821
NM_006962ZNF1821
NM_017612ZCCHC81
NM_080664C14orf1261
NM_003359UGDH1
NM_213599TMEM16E1

Immunostimulatory motif search results


UGUGU - Judge et al. Sequence-dependent stimulation of the mammalian innate immune response by synthetic siRNA. Nat Biotechnol. 2005 Apr;23(4):457-62. (PMID: 15778705)
No Immunostimulatory motif found (UGUGU).

GUCCUUCAA - Hornung et al. Sequence-specific potent induction of IFN-alpha by short interfering RNA in plasmacytoid dendritic cells through TLR7. Nat Med. 2005 Mar;11(3):263-70. Epub 2005 Feb 20. (PMID: 15723075)
No Immunostimulatory motif found (GUCCUUCAA).



Settings used for search

Scores were calculated using scoring scheme SS1
Using AVG scoring mode.

Hit scores

ParameterSpec. Score cut-offColourComment
Hit to same geneColourHits to the same accession or genes with 100 percent identity (i.e. likely transcript variants). It is advisable to always check the genes hit.
High scoring hit≥80ColourHits are very likely to cause an off-target effect
Medium scoring hit<80¸≥40ColourHits with a small chance of causing an off target effect.
Low scoring hitColourHits very unlikely to cause an off-target effect

The mismatches are colour coded based on the weighting of that position.

Cut-offColour
MatchColour
Wobble base pairs (G-U)Colour
Critical bases (none)Colour
0.6 bitsColour
0.4 bitsColour
0.2 bitsColour

About SpecificityServer

SpecificityServer is designed to help you identify potential non-specific matches to your siRNA. It incorporates the latest information about non-specific matches (sequence-specific only).

Designed by: Alistair Chalk, Quan Du, Zicai Liang and Erik L.L. Sonnhammer


For more details about the algorithm "siRNA specificity searching incorporating mismatch tolerance data" Bioinformatics (2008) 24 (10): 1316-1317