VIRsiRNAdb

SpecificityServer - An siRNA design program for determining siRNA specificity
Automatic recommendation
OK - siRNA is specific for both sense and antisense (guide) strands

35 hits were found for GGUCAACAGCAAUGCAGCU (7 guide strand hits and 28 sense strand hits.)

Hits to Guide strand

Accession

Gene

Start

End

Details of mismatches

Spec. Score

Mismatches

Contiguous

Jackson style specificity

Hits similar gene

HSP Score

HSP % Identity

HSP length

Gene Length

1

2

3

4

5

6

7

8

9

10

11

12

13

14

15

16

17

18

19

NM_001125

ADPRH

200

218

G

G

c

C

A

A

C

A

G

C

A

g

U

G

C

A

G

C

U

46

2

8

false

<90%

3461

NM_005902

SMAD3

1126

1144

u

G

U

C

A

A

C

A

G

g

A

A

U

G

C

A

G

C

a

10

3

8

bc

false

<90%

6256

NM_001033602

KIAA0774

136

154

G

G

a

C

A

A

C

A

G

a

A

A

U

G

C

A

G

C

a

-7

3

8

bc

false

<90%

6957

NM_198596

SULF2

984

1002

G

G

U

C

A

u

C

A

G

C

c

A

U

G

C

A

G

C

c

-29

3

7

c

false

<90%

3843

NM_018837

SULF2

984

1002

G

G

U

C

A

u

C

A

G

C

c

A

U

G

C

A

G

C

c

-29

3

7

c

false

<90%

3897

XM_001126750

ABI2

3977

3995

G

G

U

C

A

g

C

A

G

g

A

A

U

G

C

A

G

C

U

-46

2

9

bc

false

<90%

6311

NM_005759

ABI2

4145

4163

G

G

U

C

A

g

C

A

G

g

A

A

U

G

C

A

G

C

U

-46

2

9

bc

false

<90%

6489

Hits to Sense strand

Accession

Gene

Start

End

Details of mismatches

Spec. Score

Mismatches

Contiguous

Jackson style specificity

Hits similar gene

HSP Score

HSP % Identity

HSP length

Gene Length

1

2

3

4

5

6

7

8

9

10

11

12

13

14

15

16

17

18

19

NM_022041

GAN

523

541

g

G

C

U

G

C

A

U

U

G

C

U

G

c

U

G

A

g

a

70

4

12

c

false

<90%

4677

NM_024642

GALNT12

1513

1531

g

G

C

U

G

C

A

U

U

G

C

U

G

U

g

G

A

a

g

65

4

13

c

false

<90%

2770

NM_020041

SLC2A9

513

531

u

G

C

U

G

C

A

U

U

G

C

U

G

a

U

G

g

C

C

54

3

12

c

false

<90%

1874

NM_001001290

SLC2A9

524

542

u

G

C

U

G

C

A

U

U

G

C

U

G

a

U

G

g

C

C

54

3

12

c

false

<90%

1885

NM_006414

RPP38

273

291

u

G

C

a

G

C

A

U

U

G

C

U

G

U

U

G

A

u

a

53

4

13

c

false

<90%

990

NM_183005

RPP38

735

753

u

G

C

a

G

C

A

U

U

G

C

U

G

U

U

G

A

u

a

53

4

13

c

false

<90%

1446

NM_001007139

IGF2

789

807

u

G

C

U

G

C

A

U

U

G

C

U

G

c

U

u

A

C

C

52

3

12

c

false

<90%

5139

NM_000612

IGF2

815

833

u

G

C

U

G

C

A

U

U

G

C

U

G

c

U

u

A

C

C

52

3

12

c

false

<90%

5165

NM_003584

DUSP11

1249

1267

A

a

u

U

G

C

A

U

U

G

C

U

G

U

U

u

A

C

C

42

3

12

c

false

<90%

1593

NM_207344

SPRYD4

350

368

A

G

C

U

G

C

A

U

U

G

g

U

G

U

U

G

A

u

g

31

3

10

c

false

<90%

1109

XM_934019

NBPF9

1896

1914

g

u

C

U

G

C

A

U

g

G

C

U

G

U

U

G

A

C

a

21

4

9

bc

false

<90%

2181

NM_001039703

NBPF10

1850

1868

g

u

C

U

G

C

A

U

g

G

C

U

G

U

U

G

A

C

a

21

4

9

bc

false

<90%

2701

NM_001037675

NBPF20

1855

1873

g

u

C

U

G

C

A

U

g

G

C

U

G

U

U

G

A

C

a

21

4

9

bc

false

<90%

2931

XM_001133097

LOC728912

2799

2817

g

u

C

U

G

C

A

U

g

G

C

U

G

U

U

G

A

C

a

21

4

9

bc

false

<90%

3194

NM_015383

NBPF14

776

794

g

u

C

U

G

C

A

U

g

G

C

U

G

U

U

G

A

C

a

21

4

9

bc

false

<90%

3689

NM_173638

NBPF15

1455

1473

g

u

C

U

G

C

A

U

g

G

C

U

G

U

U

G

A

C

a

21

4

9

bc

false

<90%

4046

XM_001133185

LOC728936

1199

1217

g

u

C

U

G

C

A

U

g

G

C

U

G

U

U

G

A

C

a

21

4

9

bc

false

<90%

4211

XM_001133188

LOC728936

1480

1498

g

u

C

U

G

C

A

U

g

G

C

U

G

U

U

G

A

C

a

21

4

9

bc

false

<90%

4492

NM_017940

NBPF1

3480

3498

g

u

C

U

G

C

A

U

g

G

C

U

G

U

U

G

A

C

a

21

4

9

bc

false

<90%

4568

XM_001133155

LOC728936

1997

2015

g

u

C

U

G

C

A

U

g

G

C

U

G

U

U

G

A

C

a

21

4

9

bc

false

<90%

4784

NM_183372

NBPF11

2217

2235

g

u

C

U

G

C

A

U

g

G

C

U

G

U

U

G

A

C

a

21

4

9

bc

false

<90%

4902

XM_001133169

LOC728936

1997

2015

g

u

C

U

G

C

A

U

g

G

C

U

G

U

U

G

A

C

a

21

4

9

bc

false

<90%

4922

XM_001133179

LOC728936

2081

2099

g

u

C

U

G

C

A

U

g

G

C

U

G

U

U

G

A

C

a

21

4

9

bc

false

<90%

5093

XM_001133191

LOC728936

2099

2117

g

u

C

U

G

C

A

U

g

G

C

U

G

U

U

G

A

C

a

21

4

9

bc

false

<90%

5111

XR_015220

LOC730476

2152

2170

g

u

C

U

G

C

A

U

g

G

C

U

G

U

U

G

A

C

a

21

4

9

bc

false

<90%

5174

XM_001133181

LOC728936

1997

2015

g

u

C

U

G

C

A

U

g

G

C

U

G

U

U

G

A

C

a

21

4

9

bc

false

<90%

5622

XR_015390

LOC728841

1249

1267

g

u

C

U

G

C

A

U

g

G

C

U

G

U

U

G

A

C

a

21

4

9

bc

false

<90%

11722

NM_016252

BIRC6

15074

15092

A

G

C

U

G

C

A

U

U

G

a

U

G

U

g

G

A

C

u

9

3

10

c

false

<90%

16079

---

3 prime UTR Seed matches

Hits to same gene : 0
Hits to other genes : 672
View matched accessions

3 prime seed match details

Accession	Gene name	Hits to this gene
NM_002737	PRKCA	3
NM_173598	KSR2	3
NM_033631	LUZP1	2
NM_016132	MYEF2	2
NM_033512	TSPYL5	2
XM_001130020	KIAA1147	2
XM_001130081	KIAA1147	2
NM_001080392	KIAA1147	2
NM_021269	ZNF708	2
NM_030962	SBF2	2
NM_001017440	CALN1	2
NM_031468	CALN1	2
XM_001132928	LOC728969	2
NM_004807	HS6ST1	2
NM_003369	UVRAG	2
NM_020466	LYRM2	2
NM_017707	DDEFL1	2
NM_001627	ALCAM	2
NM_173495	PTCHD1	2
NM_021949	ATP2B3	2
NM_001001344	ATP2B3	2
NM_002486	NCBP1	2
NM_021813	BACH2	2
NM_178457	C20orf174	2
NM_022351	EFCBP1	2
NM_002667	PLN	1
NM_006496	GNAI3	1
NM_016653	ZAK	1
NM_002009	FGF7	1
NM_005741	ZNF263	1
NM_001100398		1
NM_015085	GARNL4	1
NM_032013	NDRG3	1
NM_022477	NDRG3	1
NM_000555	DCX	1
NM_178152	DCX	1
NM_178153	DCX	1
NM_178151	DCX	1
NM_001031749	LYPD5	1
NM_182573	LYPD5	1
NM_181705	LYRM7	1
NM_012455	PSD4	1
NM_152316	C11orf46	1
NM_138568	EXOC3L2	1
NM_005989	AKR1D1	1
NM_006390	IPO8	1
NM_014411	NAG8	1
NM_015038		1
NM_014862	ARNT2	1
NM_004100	EYA4	1
NM_172105	EYA4	1
NM_172103	EYA4	1
NM_019045	WDR44	1
XM_930750	LOC642366	1
XM_943652	LOC642366	1
NM_001033055	AIPL1	1
NM_001033054	AIPL1	1
NM_014336	AIPL1	1
XM_001127145	LOC728310	1
XM_001131704	LOC729900	1
NM_021936	PAPPA2	1
NM_001981	EPS15	1
NM_020432	PHTF2	1
NM_000090	COL3A1	1
NM_024761	MOBKL2B	1
NM_004464	FGF5	1
NM_033143	FGF5	1
NM_019021	C11orf71	1
NM_001004298	C10orf90	1
NM_033449	FCHSD1	1
XM_938725	LOC151234	1
XM_930027	LOC151234	1
NM_006091	CORO2B	1
NM_001013620	ALG10B	1
NM_001008710	RBPMS	1
NM_001008711	RBPMS	1
NM_006867	RBPMS	1
XM_936640	LOC647589	1
NM_182759	FAM19A3	1
NM_001004440	FAM19A3	1
NM_001032282	KLF10	1
NM_005655	KLF10	1
NM_001081573	GAB3	1
NM_080612	GAB3	1
NM_015059	TLN2	1
NM_001007527	LMBRD2	1
NM_032189	ATP11A	1
NM_015205	ATP11A	1
NM_003559	PIP5K2B	1
NM_014653	WSCD2	1
NM_145015	MRGPRF	1
NM_001098515		1
NM_207410	GFRAL	1
NM_030761	WNT4	1
NM_001402	EEF1A1	1
NM_003112	SP4	1
NM_000317	PTS	1
NM_014251	SLC25A13	1
NM_152395	NUDT16	1
NM_207313	TMEM132E	1
NM_001078173	FAM127C	1
NM_001024455	RGAG4	1
NM_017831	RNF125	1
NM_003234	TFRC	1
NM_207037	TCF12	1
NM_003205	TCF12	1
NM_207040	TCF12	1
NM_207036	TCF12	1
NM_207038	TCF12	1
NM_001271	CHD2	1
NM_015381	FAM19A5	1
NM_001082967	FAM19A5	1
NM_172005	WFDC13	1
NM_031426	C9orf58	1
NM_004319	ASTN1	1
NM_207361	FREM2	1
NM_052962	IL22RA2	1
NM_181309	IL22RA2	1
NM_181310	IL22RA2	1
NM_017759	FLJ20309	1
XM_939284	LOC650200	1
NM_004349	RUNX1T1	1
NM_175634	RUNX1T1	1
NM_175635	RUNX1T1	1
NM_175636	RUNX1T1	1
NM_001101669		1
NM_003866	INPP4B	1
NM_001616	ACVR2A	1
NM_000376	VDR	1
NM_001017535	VDR	1
NM_001001349	NKIRAS2	1
NM_017595	NKIRAS2	1
NM_144639	UROC1	1
NM_152361	EID2B	1
NM_001003940	BMF	1
NM_001003943	BMF	1
NM_001003942	BMF	1
NM_033503	BMF	1
NM_181079	IL21R	1
NM_021798	IL21R	1
NM_181078	IL21R	1
NM_181713	UBXD4	1
NM_000046	ARSB	1
NM_002111	HD	1
NM_033238	PML	1
NM_015354	NUP188	1
NM_020927	KIAA1576	1
NM_001113239		1
NM_022740	HIPK2	1
NM_002886	RAP2B	1
NM_005056	JARID1A	1
NM_001042603	JARID1A	1
NM_000585	IL15	1
NM_172174	IL15	1
NM_006224	PITPNA	1
NM_015055	SWAP70	1
NM_032901	C12orf62	1
NM_018941	CLN8	1
XM_001127632	LOC728519	1
NM_004536	NAIP	1
NM_022892	NAIP	1
NM_004145	MYO9B	1
NM_017436	A4GALT	1
NM_201266	NRP2	1
NM_003872	NRP2	1
NM_201279	NRP2	1
NM_006843	SDS	1
NM_004852	ONECUT2	1
NM_003028	SHB	1
NM_130848	C5orf20	1
NM_152455	ZSCAN29	1
NM_003382	VIPR2	1
NM_003473	STAM	1
NM_003786	ABCC3	1
NM_023016	ANKRD57	1
NM_153748	KCNC2	1
NM_139136	KCNC2	1
NM_003983	SLC7A6	1
NM_001076785	SLC7A6	1
XM_001134215	PDPR	1
XM_375929	LOC400856	1
NM_001002844	SUHW4	1
NM_003822	NR5A2	1
NM_205860	NR5A2	1
NM_173167	UNC45B	1
NM_001033576	UNC45B	1
XM_001134449	KBTBD9	1
XM_940375	KBTBD9	1
NM_080671	KCNE4	1
NM_001044722	CSNK1G3	1
NM_001031812	CSNK1G3	1
NM_001044723	CSNK1G3	1
NM_004384	CSNK1G3	1
NM_003474	ADAM12	1
NM_022828	YTHDC2	1
XM_940609	FLJ25076	1
XM_059689	FLJ25076	1
NM_024585	ARMC7	1
NM_007195	POLI	1
NM_031965	GSG2	1
XM_932529	LOC644949	1
XM_943887	LOC644949	1
NM_001099	ACPP	1
NM_152695	ZNF449	1
NM_198992	SYT10	1
NM_173854	SLC41A1	1
NM_152339	SPATA2L	1
NM_006544	EXOC5	1
XM_942952	FLJ46284	1
XM_499123	FLJ46284	1
NM_080415	SEPT4	1
NM_178140	PDZD2	1
NM_004300	ACP1	1
NM_007099	ACP1	1
NM_001039362	ATP6V1C2	1
NM_144583	ATP6V1C2	1
NM_001767	CD2	1
NM_177549	PRNT	1
NM_000546	TP53	1
NM_021167	GATAD1	1
NM_004375	COX11	1
NM_199461	NANOS1	1
NM_018429	BDP1	1
NM_182515	ZNF714	1
NM_019855	CABP5	1
NM_024771	NAT11	1
NM_173214	NFAT5	1
NM_006599	NFAT5	1
NM_138714	NFAT5	1
NM_001113178		1
NM_138713	NFAT5	1
NM_025147	COQ10B	1
NM_018947	CYCS	1
NM_173494	CXorf41	1
NM_001080497	MEGF9	1
XM_936860	MEGF9	1
NM_052845	MMAB	1
NM_001013659	ZNF793	1
NM_207511	C9orf139	1
NM_006974	ZNF33A	1
NM_006954	ZNF33A	1
NM_021116	ADCY1	1
NM_018292	QRSL1	1
NM_000572	IL10	1
NM_181305	MRPL52	1
NM_181307	MRPL52	1
NM_180982	MRPL52	1
NM_181304	MRPL52	1
NM_178336	MRPL52	1
NM_181306	MRPL52	1
NM_014829	DDX46	1
NM_001024680	LOC554251	1
NM_015174	KIAA1086	1
XM_001133059	LOC728772	1
XM_001133074	LOC728772	1
XM_001133066	LOC728772	1
NM_145041	TMEM106A	1
NM_006852	TLK2	1
NM_001112707		1
NM_005497	GJA7	1
NM_001080383	GJA7	1
NM_001007525	NWD1	1
NM_004486	GOLGA2	1
NM_002740	PRKCI	1
NM_152916	EMR2	1
NM_152918	EMR2	1
NM_152917	EMR2	1
NM_152920	EMR2	1
NM_152921	EMR2	1
NM_013447	EMR2	1
NM_152919	EMR2	1
NM_021030	ZNF14	1
NM_020462	ERGIC1	1
NM_198269	HIPK1	1
NM_181358	HIPK1	1
NM_198268	HIPK1	1
NM_147202	C9orf25	1
NM_148174	AZIN1	1
NM_015878	AZIN1	1
NM_173496	MPP7	1
NM_033439	IL33	1
NM_014625	NPHS2	1
NM_058179	PSAT1	1
NM_021154	PSAT1	1
NM_054110	GALNTL2	1
NM_018316	KLHL26	1
NM_006750	SNTB2	1
NM_001042646	TRAK1	1
NM_021133	RNASEL	1
NM_000875	IGF1R	1
NM_003339	UBE2D2	1
NM_181838	UBE2D2	1
NM_153769	CABYR	1
NM_012189	CABYR	1
NM_138644	CABYR	1
NM_138643	CABYR	1
NM_153770	CABYR	1
NM_153768	CABYR	1
NM_005338	HIP1	1
NM_001511	CXCL1	1
NM_002089	CXCL2	1
NM_017946	FKBP14	1
NM_018085	IPO9	1
NM_177550	SLC13A5	1
NM_001080540	LOC389293	1
XM_371741	LOC389293	1
XM_942792	LOC642170	1
NM_001001971	FAM13C1	1
NM_198215	FAM13C1	1
NM_030940	ISCA1	1
NM_007036	ESM1	1
NM_198555	ANKRD36	1
XM_001130291	LOC730995	1
NM_031243	HNRPA2B1	1
NM_002137	HNRPA2B1	1
NM_015848	KRT76	1
NM_014978	SORCS3	1
NM_005370	RAB8A	1
NM_013276	CARKL	1
NM_031890	CECR6	1
NM_020792	AADACL1	1
XM_942239	LOC150084	1
NM_001080444	LOC150084	1
NM_080669	SLC46A1	1
NM_052848	CCDC97	1
NM_182830	MDGA2	1
NM_000108	DLD	1
NM_178571	MGC51025	1
XM_934743	FBXO46	1
XM_946240	FBXO46	1
NM_001080469	FBXO46	1
XM_946239	FBXO46	1
NM_000742	CHRNA2	1
NM_080872	UNC5D	1
NM_001012958	DISC1	1
XM_931281	LOC643072	1
XM_943769	LOC643072	1
NM_021205	RHOU	1
NM_173689	CRB2	1
NM_004586	RPS6KA3	1
NM_015831	ACHE	1
NM_153620	HOXA1	1
NM_007050	PTPRT	1
NM_133170	PTPRT	1
NM_032296	FLYWCH1	1
NM_002182	IL1RAP	1
NM_022369	STRA6	1
NM_207417	FLJ46082	1
NM_024791	PDZD3	1
NM_052924	RHPN1	1
NM_020921	NIN	1
NM_170665	ATP2A2	1
NM_006946	SPTBN2	1
NM_016376	ANKFY1	1
NM_018411	HR	1
NM_005144	HR	1
NM_024017	HOXB9	1
NM_152787	MAP3K7IP3	1
NM_001099289		1
NM_183373	C6orf145	1
XM_937537	C6orf145	1
NM_006158	NEFL	1
NM_015284	KIAA0467	1
NM_014374	REPIN1	1
NM_001099696		1
NM_013400	REPIN1	1
NM_001099695		1
NM_018518	MCM10	1
NM_182751	MCM10	1
NM_014680	KIAA0100	1
NM_001099694		1
NM_138972	BACE1	1
NM_012104	BACE1	1
NM_138973	BACE1	1
NM_138971	BACE1	1
NM_003170	SUPT6H	1
NM_130446	KLHL6	1
NM_001004106	GRK6	1
NM_002082	GRK6	1
NM_002207	ITGA9	1
NM_019891	ERO1LB	1
NM_002221	ITPKB	1
NM_005178	BCL3	1
NM_025187	C16orf70	1
NM_001083924	C1orf116	1
NM_023938	C1orf116	1
NM_002126	HLF	1
NM_003247	THBS2	1
NM_020946	DENND1A	1
NM_002999	SDC4	1
NM_003898	SYNJ2	1
NM_017704	ANKRD49	1
NM_016011	MECR	1
NM_001024732	MECR	1
NM_014005	PCDHA9	1
NM_001421	ELF4	1
XM_001127087	FLJ43950	1
NM_145177	DHRSX	1
NM_001040280	CD83	1
NM_004233	CD83	1
XM_001129120	LOC729034	1
NM_145159	JAG2	1
NM_002226	JAG2	1
NM_017656	ZNF562	1
NM_002655	PLAG1	1
NM_001039570	KREMEN1	1
NM_001039571	KREMEN1	1
NM_016529	ATP8A2	1
NM_152295	TARS	1
NM_015879	ST8SIA3	1
XM_001129019	SAPS2	1
NM_002990	CCL22	1
NM_181425	FXN	1
NM_000144	FXN	1
NM_023077	C1orf163	1
NM_198087	ZNF200	1
NM_198088	ZNF200	1
NM_003454	ZNF200	1
NM_022803	UCP3	1
NM_001012729	DUXA	1
NM_001030288	SPN	1
NM_003123	SPN	1
NM_004709	CXorf1	1
NM_013279	C11orf9	1
NM_032875	FBXL20	1
NM_015570	AUTS2	1
NM_005282	GPR4	1
NM_003039	SLC2A5	1
NM_024666	FLJ11506	1
NM_198066	GNPNAT1	1
NM_005520	HNRPH1	1
NM_015659	RSL1D1	1
NM_033225	CSMD1	1
NM_001003652	SMAD2	1
NM_005901	SMAD2	1
NM_052811	TRIM13	1
NM_213590	TRIM13	1
NM_001007278	TRIM13	1
NM_005798	TRIM13	1
NM_199227	MAP1D	1
NM_015064	ERC1	1
NM_178038	ERC1	1
NM_178037	ERC1	1
XM_929970	TREML3	1
XM_941488	TREML3	1
XM_943286	LOC648354	1
XM_931676	LOC643599	1
NM_006762	LAPTM5	1
NM_020724	RNF150	1
NM_005994	TBX2	1
NM_014907	FRMPD1	1
NM_004968	ICA1	1
NM_022307	ICA1	1
NM_002062	GLP1R	1
NM_017488	ADD2	1
NM_139277	KLK7	1
NM_005046	KLK7	1
NM_001043318	C14orf43	1
NM_194278	C14orf43	1
NM_020911		1
NM_020393	PGLYRP4	1
NM_013999	MEOX1	1
NM_004527	MEOX1	1
NM_001040002	MEOX1	1
NM_022462	HIF3A	1
NM_152794	HIF3A	1
NM_152795	HIF3A	1
NM_052839	PANX2	1
NM_018706	DHTKD1	1
NM_017817	RAB20	1
NM_001024843	TNRC6B	1
NM_015088	TNRC6B	1
XM_376795	FLJ45872	1
XM_940037	FLJ45872	1
NM_053279	C8orf13	1
NM_212558	LOC401498	1
NM_152417	TMEM68	1
NM_014313	TMEM50A	1
NM_001100162		1
NM_001100161		1
NM_015024	XPO7	1
NM_022470	ZMAT3	1
NM_152240	ZMAT3	1
NM_006777	ZBTB33	1
XM_001131019	LOC731846	1
XM_001131201	LOC729751	1
NM_001701	BAAT	1
NM_001918	DBT	1
NM_014663	JMJD2A	1
NM_197941	ADAMTS6	1
NM_139155	ADAMTS14	1
NM_080722	ADAMTS14	1
NM_012081	ELL2	1
XM_001131744	LOC732060	1
XM_001126476	LOC728073	1
NM_182493	MLCK	1
NM_020700		1
NM_001804	CDX1	1
NM_032800	C1orf198	1
NM_207395	ZNF324B	1
NM_199459	C10orf71	1
NM_001081461	JMJD6	1
NM_005044	PRKX	1
NM_002760	PRKY	1
NM_001103	ACTN2	1
NM_001079528	ABCC6	1
XM_001132078	LOC730013	1
NM_001430	EPAS1	1
NM_014959	CARD8	1
NM_000738	CHRM1	1
NM_019020	TBC1D16	1
NM_032515	BOK	1
NM_002868	RAB5B	1
NM_138635	H2AFV	1
NM_138402	LOC93349	1
NM_152744	SDK1	1
NM_006805	HNRPA0	1
NM_002318	LOXL2	1
NM_001098632		1
NM_032375	AKT1S1	1
NM_001098633		1
NM_021192	HOXD11	1
NM_001003676	C11orf49	1
NM_001412	EIF1AX	1
NM_006933	SLC5A3	1
NM_032907	UBL7	1
NM_201265	UBL7	1
NM_005504	BCAT1	1
NM_000451	SHOX	1
NM_018557	LRP1B	1
NM_001754	RUNX1	1
NM_001001890	RUNX1	1
NM_018011	FLJ10154	1
NM_018400	SCN3B	1
NM_001040151	SCN3B	1
NM_024569	MPZL1	1
NM_003953	MPZL1	1
NM_002560	P2RX4	1
NM_012199	EIF2C1	1
NM_001079526	IKZF2	1
NM_016260	IKZF2	1
NM_000147	FUCA1	1
NM_138333	FAM122A	1
NM_145739	OSBPL6	1
NM_032523	OSBPL6	1
NM_001018058	TFEC	1
NM_012252	TFEC	1
NM_021629	GNB4	1
NM_015046	SETX	1
NM_022492	TTC31	1
NM_002397	MEF2C	1
NM_005562	LAMC2	1
NM_183078	RNF8	1
NM_003958	RNF8	1
NM_000183	HADHB	1
XM_932965	LOC645553	1
XM_945010	LOC645553	1
NM_007137	ZNF81	1
NM_033390		1
NM_170672	RASGRP3	1
NM_001047	SRD5A1	1
NM_022091	ASCC3	1
NM_020361	CPA6	1
XM_932563	LOC643008	1
XM_942645	LOC643008	1
XM_928053	LOC643008	1
XM_946181	LOC643008	1
XM_001132247	C1orf108	1
NM_198337	INSIG1	1
NM_005542	INSIG1	1
NM_198336	INSIG1	1
NM_019030	DHX29	1
NM_017966	VPS37C	1
NM_020210	SEMA4B	1
NM_198925	SEMA4B	1
NM_018071	FLJ10357	1
NM_198353	KCTD8	1
NM_002677	PMP2	1
NM_004830	CRSP3	1
NM_017413	APLN	1
NM_000351	STS	1
NM_001099404		1
NM_198056	SCN5A	1
NM_001099405		1
NM_000335	SCN5A	1
NM_173582	PGM2L1	1
NM_020474	GALNT1	1
NM_001018067	SERBP1	1
NM_015640	SERBP1	1
NM_001018069	SERBP1	1
NM_001018068	SERBP1	1
NM_016009	SH3GLB1	1
NM_001102396		1
NM_025073	SIKE	1
NM_033296	MRFAP1	1
NM_016448	DTL	1
NM_004845	PCYT1B	1
NM_001008388	ZCD2	1
NM_014551	NCAPH2	1
NM_152529	GPR155	1
NM_001033045	GPR155	1
NM_014930	ZNF510	1
NM_020717	SHROOM4	1
NM_139246	C9orf97	1
NM_033428	C9orf123	1
NM_018203	KLHDC8A	1
NM_004804	CIAO1	1
NM_003290	TPM4	1
NM_207406	CCDC4	1
NM_013995	LAMP2	1
NM_000719	CACNA1C	1
NM_004654	USP9Y	1
NM_078470	COX15	1
NM_144767	AKAP13	1
NM_006738	AKAP13	1
NM_007200	AKAP13	1
NM_000994	RPL32	1
NM_001007074	RPL32	1
NM_001007073	RPL32	1
NM_006265	RAD21	1
NM_005739	RASGRP1	1
NM_020177	FEM1C	1
NM_012193	FZD4	1
NM_014309	RBM9	1
NM_001082576	RBM9	1
NM_001082577	RBM9	1
NM_001031695	RBM9	1
NM_001082578	RBM9	1
NM_001082579	RBM9	1
NM_031463	HSDL1	1
NM_005191	CD80	1
NM_022762	RMND5B	1
NM_032547	SCOC	1
NM_032317	WBSCR18	1
NM_032765	TRIM52	1
NM_058169	LOH12CR1	1
NM_004331	BNIP3L	1
NM_023112	OTUB2	1
NM_003486	SLC7A5	1
NM_007107	SSR3	1
NM_001033056	GLUL	1
NM_002065	GLUL	1
NM_001033044	GLUL	1
NM_015278	SASH1	1
NM_006833	COPS6	1
NM_022459	XPO4	1
NM_006741	PPP1R1A	1
NM_175921	LOC285636	1
NM_024544	C1orf166	1
NM_001008737	LOC401052	1
NM_017762	MTMR10	1
NM_003270	TSPAN6	1
NM_173851	SLC30A8	1
NM_014112	TRPS1	1
NM_003913	PRPF4B	1
NM_130767	ACOT12	1
XM_939100	MYO5B	1
NM_001080467	MYO5B	1
NM_004797	ADIPOQ	1
NM_001012515	FECH	1
NM_000140	FECH	1
NM_001099691		1
NM_003236	TGFA	1
XM_001134139	LOC144481	1
XM_001128090	LOC144481	1
NM_000332	ATXN1	1
NM_024836	ZNF672	1
NM_006060	IKZF1	1
NM_138638	CFL2	1
NM_021914	CFL2	1
NM_021140	UTX	1
NM_174878	CLRN1	1

---

Immunostimulatory motif search results

UGUGU - Judge et al. Sequence-dependent stimulation of the mammalian innate immune response by synthetic siRNA. Nat Biotechnol. 2005 Apr;23(4):457-62. (PMID: 15778705)
No Immunostimulatory motif found (UGUGU).

GUCCUUCAA - Hornung et al. Sequence-specific potent induction of IFN-alpha by short interfering RNA in plasmacytoid dendritic cells through TLR7. Nat Med. 2005 Mar;11(3):263-70. Epub 2005 Feb 20. (PMID: 15723075)
No Immunostimulatory motif found (GUCCUUCAA).

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Settings used for search

Scores were calculated using scoring scheme SS1
Using AVG scoring mode.

Hit scores

Parameter	Spec. Score cut-off	Colour	Comment
Hit to same gene		Colour	Hits to the same accession or genes with 100 percent identity (i.e. likely transcript variants). It is advisable to always check the genes hit.
High scoring hit	≥80	Colour	Hits are very likely to cause an off-target effect
Medium scoring hit	<80¸≥40	Colour	Hits with a small chance of causing an off target effect.
Low scoring hit		Colour	Hits very unlikely to cause an off-target effect

The mismatches are colour coded based on the weighting of that position.

Cut-off	Colour
Match	Colour
Wobble base pairs (G-U)	Colour
Critical bases (none)	Colour
0.6 bits	Colour
0.4 bits	Colour
0.2 bits	Colour

About SpecificityServer

SpecificityServer is designed to help you identify potential non-specific matches to your siRNA. It incorporates the latest information about non-specific matches (sequence-specific only).

Designed by: Alistair Chalk, Quan Du, Zicai Liang and Erik L.L. Sonnhammer

For more details about the algorithm "siRNA specificity searching incorporating mismatch tolerance data" Bioinformatics (2008) 24 (10): 1316-1317