Automatic recommendation
OK - siRNA is specific for both sense and antisense (guide) strands
10 hits were found for GGUCACCUUUGACAGACUG (5 guide strand hits and 5 sense strand hits.)
Hits to Guide strand
| Accession | Gene | Start | End | Details of mismatches | Spec. Score | Mismatches | Contiguous | Jackson style specificity | Hits similar gene | HSP Score | HSP % Identity | HSP length | Gene Length | ||||||||||||||||||
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | |||||||||||||
| NM_182486 | C1QTNF6 | 212 | 230 | G | c | U | C | A | C | C | U | U | U | G | A | C | A | G | A | g | c | u | 57 | 4 | 14 | ac | false | <90% | 1670 | ||
| NM_031910 | C1QTNF6 | 212 | 230 | G | c | U | C | A | C | C | U | U | U | G | A | C | A | G | A | g | c | u | 57 | 4 | 14 | ac | false | <90% | 2954 | ||
| NM_002039 | GAB1 | 2986 | 3004 | u | u | g | C | A | C | C | U | U | U | G | A | a | A | G | A | C | U | G | 56 | 4 | 9 | false | <90% | 3323 | |||
| NM_207123 | GAB1 | 3076 | 3094 | u | u | g | C | A | C | C | U | U | U | G | A | a | A | G | A | C | U | G | 56 | 4 | 9 | false | <90% | 3413 | |||
| NM_198150 | ARSK | 889 | 907 | G | G | U | C | A | C | C | U | U | U | G | u | C | A | G | A | a | a | u | 35 | 4 | 11 | false | <90% | 3345 | |||
Hits to Sense strand
| Accession | Gene | Start | End | Details of mismatches | Spec. Score | Mismatches | Contiguous | Jackson style specificity | Hits similar gene | HSP Score | HSP % Identity | HSP length | Gene Length | ||||||||||||||||||
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | |||||||||||||
| NM_003243 | TGFBR3 | 1688 | 1706 | C | u | G | U | C | U | G | U | C | A | A | A | u | G | U | G | A | C | a | 73 | 3 | 10 | false | <90% | 4199 | |||
| NM_172364 | CACNA2D4 | 3467 | 3485 | g | c | c | U | C | U | G | U | C | A | A | A | u | G | U | G | A | C | C | 56 | 4 | 9 | false | <90% | 5343 | |||
| NM_015989 | CSAD | 1613 | 1631 | a | A | G | g | C | U | G | U | C | A | A | A | G | G | U | G | g | C | C | 37 | 3 | 12 | c | false | <90% | 2648 | ||
| NM_024857 | ATAD5 | 2491 | 2509 | C | A | G | U | a | g | G | U | C | A | A | A | G | G | U | G | A | C | u | -19 | 3 | 12 | bc | false | <90% | 6245 | ||
| XM_001134002 | C17orf41 | 2512 | 2530 | C | A | G | U | a | g | G | U | C | A | A | A | G | G | U | G | A | C | u | -19 | 3 | 12 | bc | false | <90% | 6783 | ||
3 prime UTR Seed matches
Hits to same gene : 0
Hits to other genes : 1357
View matched accessions
3 prime seed match details
Immunostimulatory motif search results
UGUGU - Judge et al. Sequence-dependent stimulation of the mammalian innate immune response by synthetic siRNA. Nat Biotechnol. 2005 Apr;23(4):457-62. (PMID: 15778705)
No Immunostimulatory motif found (UGUGU).
GUCCUUCAA - Hornung et al. Sequence-specific potent induction of IFN-alpha by short interfering RNA in plasmacytoid dendritic cells through TLR7. Nat Med. 2005 Mar;11(3):263-70. Epub 2005 Feb 20. (PMID: 15723075)
No Immunostimulatory motif found (GUCCUUCAA).
Settings used for search
Scores were calculated using scoring scheme SS1
Using AVG scoring mode.
Hit scores
| Parameter | Spec. Score cut-off | Colour | Comment |
| Hit to same gene | Colour | Hits to the same accession or genes with 100 percent identity (i.e. likely transcript variants). It is advisable to always check the genes hit. | |
| High scoring hit | ≥80 | Colour | Hits are very likely to cause an off-target effect |
| Medium scoring hit | <80¸≥40 | Colour | Hits with a small chance of causing an off target effect. |
| Low scoring hit | Colour | Hits very unlikely to cause an off-target effect |
The mismatches are colour coded based on the weighting of that position.
| Cut-off | Colour |
| Match | Colour |
| Wobble base pairs (G-U) | Colour |
| Critical bases (none) | Colour |
| 0.6 bits | Colour |
| 0.4 bits | Colour |
| 0.2 bits | Colour |
SpecificityServer is designed to help you identify potential non-specific matches to your siRNA. It incorporates the latest information about non-specific matches (sequence-specific only).
Designed by: Alistair Chalk, Quan Du, Zicai Liang and Erik L.L. Sonnhammer