Automatic recommendation
OK - siRNA is specific for both sense and antisense (guide) strands
18 hits were found for GAAAGGGGAGCAUUGACAC (11 guide strand hits and 7 sense strand hits.)
Hits to Guide strand
| Accession | Gene | Start | End | Details of mismatches | Spec. Score | Mismatches | Contiguous | Jackson style specificity | Hits similar gene | HSP Score | HSP % Identity | HSP length | Gene Length | ||||||||||||||||||
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | |||||||||||||
| XR_016061 | LOC732173 | 422 | 440 | G | A | A | A | G | c | G | G | A | G | C | A | U | U | G | A | g | g | a | 5 | 4 | 10 | c | false | <90% | 1838 | ||
| XM_927767 | LOC653505 | 1598 | 1616 | G | A | A | A | G | G | a | G | A | G | C | c | U | U | G | A | C | A | u | -5 | 3 | 6 | false | <90% | 3854 | |||
| XM_928338 | LOC653598 | 1598 | 1616 | G | A | A | A | G | G | a | G | A | G | C | c | U | U | G | A | C | A | u | -5 | 3 | 6 | false | <90% | 3854 | |||
| XM_927706 | LOC644591 | 1599 | 1617 | G | A | A | A | G | G | a | G | A | G | C | c | U | U | G | A | C | A | u | -5 | 3 | 6 | false | <90% | 3856 | |||
| NM_000662 | NAT1 | 150 | 168 | G | A | A | A | G | G | G | G | A | u | C | A | U | g | G | A | C | A | u | -7 | 3 | 9 | c | false | <90% | 1438 | ||
| NM_182511 | CBLN2 | 2411 | 2429 | G | A | A | A | G | G | G | a | u | G | C | A | U | U | G | A | C | A | C | -22 | 2 | 10 | bc | false | <90% | 2750 | ||
| NM_003429 | ZNF85 | 111 | 129 | G | A | A | A | u | G | G | G | A | c | C | A | U | U | G | A | C | A | u | -39 | 3 | 8 | bc | false | <90% | 2315 | ||
| NM_001001415 | ZNF429 | 135 | 153 | G | A | A | A | u | G | G | G | A | c | C | A | U | U | G | A | C | A | u | -39 | 3 | 8 | bc | false | <90% | 2361 | ||
| NM_021269 | ZNF708 | 196 | 214 | G | A | A | A | u | G | G | G | A | c | C | A | U | U | G | A | C | A | u | -39 | 3 | 8 | bc | false | <90% | 4003 | ||
| NM_003423 | ZNF43 | 128 | 146 | G | A | A | A | u | G | G | G | A | c | C | A | U | U | G | A | C | A | u | -39 | 3 | 8 | bc | false | <90% | 5225 | ||
| NM_007153 | ZNF208 | 147 | 165 | G | A | A | A | u | G | G | G | A | u | C | A | U | U | G | A | C | A | u | -39 | 3 | 8 | bc | false | <90% | 8241 | ||
Hits to Sense strand
| Accession | Gene | Start | End | Details of mismatches | Spec. Score | Mismatches | Contiguous | Jackson style specificity | Hits similar gene | HSP Score | HSP % Identity | HSP length | Gene Length | ||||||||||||||||||
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | |||||||||||||
| XM_001128450 | LOC728229 | 2523 | 2541 | u | U | u | U | C | A | A | U | G | C | U | C | C | C | C | U | U | U | C | 78 | 2 | 16 | bc | false | <90% | 2916 | ||
| XM_001131298 | LOC730990 | 2526 | 2544 | u | U | u | U | C | A | A | U | G | C | U | C | C | C | C | U | U | U | C | 78 | 2 | 16 | bc | false | <90% | 2919 | ||
| XM_001128423 | LOC728229 | 2553 | 2571 | u | U | u | U | C | A | A | U | G | C | U | C | C | C | C | U | U | U | C | 78 | 2 | 16 | bc | false | <90% | 2946 | ||
| XM_928466 | LOC645426 | 2556 | 2574 | u | U | u | U | C | A | A | U | G | C | U | C | C | C | C | U | U | U | C | 78 | 2 | 16 | bc | false | <90% | 2949 | ||
| NM_014690 | FAM131B | 673 | 691 | c | a | u | U | C | A | A | U | G | C | U | C | C | C | C | U | U | c | C | 64 | 4 | 14 | c | false | <90% | 5859 | ||
| NM_145693 | LPIN1 | 198 | 216 | c | c | u | c | C | A | A | U | G | C | U | C | C | C | C | U | U | U | C | 30 | 4 | 15 | bc | false | <90% | 5363 | ||
| NM_001992 | F2R | 798 | 816 | G | U | G | U | C | u | g | U | G | C | U | C | C | C | C | U | U | U | a | -34 | 3 | 11 | bc | false | <90% | 3592 | ||
3 prime UTR Seed matches
Hits to same gene : 0
Hits to other genes : 2736
View matched accessions
3 prime seed match details
Immunostimulatory motif search results
UGUGU - Judge et al. Sequence-dependent stimulation of the mammalian innate immune response by synthetic siRNA. Nat Biotechnol. 2005 Apr;23(4):457-62. (PMID: 15778705)
No Immunostimulatory motif found (UGUGU).
GUCCUUCAA - Hornung et al. Sequence-specific potent induction of IFN-alpha by short interfering RNA in plasmacytoid dendritic cells through TLR7. Nat Med. 2005 Mar;11(3):263-70. Epub 2005 Feb 20. (PMID: 15723075)
No Immunostimulatory motif found (GUCCUUCAA).
Settings used for search
Scores were calculated using scoring scheme SS1
Using AVG scoring mode.
Hit scores
| Parameter | Spec. Score cut-off | Colour | Comment |
| Hit to same gene | Colour | Hits to the same accession or genes with 100 percent identity (i.e. likely transcript variants). It is advisable to always check the genes hit. | |
| High scoring hit | ≥80 | Colour | Hits are very likely to cause an off-target effect |
| Medium scoring hit | <80¸≥40 | Colour | Hits with a small chance of causing an off target effect. |
| Low scoring hit | Colour | Hits very unlikely to cause an off-target effect |
The mismatches are colour coded based on the weighting of that position.
| Cut-off | Colour |
| Match | Colour |
| Wobble base pairs (G-U) | Colour |
| Critical bases (none) | Colour |
| 0.6 bits | Colour |
| 0.4 bits | Colour |
| 0.2 bits | Colour |
SpecificityServer is designed to help you identify potential non-specific matches to your siRNA. It incorporates the latest information about non-specific matches (sequence-specific only).
Designed by: Alistair Chalk, Quan Du, Zicai Liang and Erik L.L. Sonnhammer