Automatic recommendation
Warning - siRNA is specific for only the antisense (guide) strand
53 hits were found for AUGCCCUGAACACCUUCAC (12 guide strand hits and 41 sense strand hits.)
Hits to Guide strand
Accession | Gene | Start | End | Details of mismatches | Spec. Score | Mismatches | Contiguous | Jackson style specificity | Hits similar gene | HSP Score | HSP % Identity | HSP length | Gene Length | ||||||||||||||||||
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | |||||||||||||
NM_000761 | CYP1A2 | 489 | 507 | A | U | G | C | C | C | U | c | A | A | C | A | C | C | U | U | C | u | C | 35 | 2 | 9 | c | false | <90% | 3127 | ||
XR_017805 | LOC401433 | 2866 | 2884 | c | U | G | C | C | C | U | G | g | A | C | A | C | C | U | U | C | c | C | 35 | 3 | 8 | c | false | <90% | 2908 | ||
XR_017935 | LOC401433 | 2866 | 2884 | c | U | G | C | C | C | U | G | g | A | C | A | C | C | U | U | C | c | C | 35 | 3 | 8 | c | false | <90% | 2908 | ||
XR_017807 | LOC401433 | 2866 | 2884 | c | U | G | C | C | C | U | G | g | A | C | A | C | C | U | U | C | c | C | 35 | 3 | 8 | c | false | <90% | 2908 | ||
NM_031303 | KATNAL2 | 589 | 607 | u | U | G | C | C | u | U | G | A | A | C | A | C | C | U | U | C | g | a | 27 | 4 | 11 | c | false | <90% | 1896 | ||
XM_001129888 | KATNAL2 | 395 | 413 | u | U | G | C | C | u | U | G | A | A | C | A | C | C | U | U | C | g | a | 27 | 4 | 11 | c | false | <90% | 2639 | ||
NM_032890 | DISP1 | 3404 | 3422 | c | U | G | C | C | C | U | G | A | c | C | A | C | C | U | U | C | g | u | 7 | 4 | 8 | c | false | <90% | 4743 | ||
NM_020452 | ATP8B2 | 3266 | 3284 | A | U | G | C | C | C | a | G | A | A | C | A | C | C | U | U | g | g | C | 7 | 3 | 9 | c | false | <90% | 5871 | ||
NM_014992 | DAAM1 | 1948 | 1966 | A | U | G | C | C | C | U | G | A | A | a | u | C | C | U | U | C | A | a | 0 | 3 | 10 | false | <90% | 4256 | |||
NM_001973 | ELK4 | 2040 | 2058 | c | c | u | C | C | C | a | G | A | A | C | A | C | C | U | U | C | A | C | 0 | 4 | 12 | bc | false | <90% | 3034 | ||
NM_021795 | ELK4 | 2040 | 2058 | c | c | u | C | C | C | a | G | A | A | C | A | C | C | U | U | C | A | C | 0 | 4 | 12 | bc | false | <90% | 3077 | ||
NM_002457 | MUC2 | 3815 | 3833 | c | c | a | C | C | C | U | G | A | c | C | A | C | C | U | U | C | A | C | -15 | 4 | 9 | bc | false | <90% | 15728 |
Hits to Sense strand
Accession | Gene | Start | End | Details of mismatches | Spec. Score | Mismatches | Contiguous | Jackson style specificity | Hits similar gene | HSP Score | HSP % Identity | HSP length | Gene Length | ||||||||||||||||||
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | |||||||||||||
NM_024779 | PIP5K2C | 127 | 145 | G | U | G | A | A | G | G | U | G | U | U | C | c | G | G | G | C | g | g | 81 | 3 | 12 | false | <90% | 2937 | |||
NM_004080 | DGKB | 3671 | 3689 | u | U | G | A | A | G | G | U | G | U | U | C | A | u | G | G | C | A | U | 78 | 2 | 12 | c | false | <90% | 3926 | ||
NM_057089 | AP1S1 | 581 | 599 | G | a | a | A | A | G | G | U | G | U | U | C | A | G | G | G | C | A | c | 64 | 3 | 15 | bc | false | <90% | 958 | ||
NM_019594 | LRRC8A | 3051 | 3069 | u | g | G | A | A | G | G | U | G | U | U | C | A | G | G | G | a | A | a | 58 | 4 | 14 | ac | false | <90% | 4296 | ||
NM_199454 | PRDM16 | 1105 | 1123 | G | U | G | A | A | G | G | U | G | U | U | C | A | c | G | G | a | c | c | 49 | 4 | 13 | c | false | <90% | 8669 | ||
NM_022114 | PRDM16 | 1105 | 1123 | G | U | G | A | A | G | G | U | G | U | U | C | A | c | G | G | a | c | c | 49 | 4 | 13 | c | false | <90% | 8726 | ||
NM_001080534 | UNC13C | 1822 | 1840 | a | U | G | g | A | G | G | U | G | U | U | C | A | G | G | G | u | A | U | 37 | 3 | 12 | c | false | <90% | 8140 | ||
XR_019418 | LOC646527 | 264 | 282 | c | U | G | A | A | G | a | U | G | U | U | C | A | G | G | G | C | A | a | 26 | 3 | 11 | bc | false | <90% | 793 | ||
XR_016632 | LOC646527 | 264 | 282 | c | U | G | A | A | G | a | U | G | U | U | C | A | G | G | G | C | A | a | 26 | 3 | 11 | bc | false | <90% | 793 | ||
XR_017214 | LOC646216 | 264 | 282 | u | U | G | A | A | G | a | U | G | U | U | C | A | G | G | G | C | A | a | 26 | 3 | 11 | bc | false | <90% | 798 | ||
XR_018933 | LOC646216 | 264 | 282 | u | U | G | A | A | G | a | U | G | U | U | C | A | G | G | G | C | A | a | 26 | 3 | 11 | bc | false | <90% | 798 | ||
XR_016663 | LOC391168 | 260 | 278 | c | U | G | A | A | G | a | U | G | U | U | C | A | G | G | G | C | A | a | 26 | 3 | 11 | bc | false | <90% | 817 | ||
XR_018601 | LOC391168 | 260 | 278 | c | U | G | A | A | G | a | U | G | U | U | C | A | G | G | G | C | A | a | 26 | 3 | 11 | bc | false | <90% | 817 | ||
XR_017247 | LOC401975 | 260 | 278 | c | U | G | A | A | G | a | U | G | U | U | C | A | G | G | G | C | A | a | 26 | 3 | 11 | bc | false | <90% | 846 | ||
XR_018444 | LOC643981 | 390 | 408 | c | U | G | A | A | G | a | U | G | U | U | C | A | G | G | G | C | A | a | 26 | 3 | 11 | bc | false | <90% | 914 | ||
XR_016514 | LOC643981 | 390 | 408 | c | U | G | A | A | G | a | U | G | U | U | C | A | G | G | G | C | A | a | 26 | 3 | 11 | bc | false | <90% | 914 | ||
XR_015860 | LOC732384 | 177 | 195 | c | U | G | A | A | G | a | U | G | U | U | C | A | G | G | G | C | A | a | 26 | 3 | 11 | bc | false | <90% | 928 | ||
XR_015517 | LOC731542 | 281 | 299 | c | U | G | A | A | G | a | U | G | U | U | C | A | G | G | G | C | A | a | 26 | 3 | 11 | bc | false | <90% | 1026 | ||
XR_015568 | LOC729510 | 281 | 299 | c | U | G | A | A | G | a | U | G | U | U | C | A | G | G | G | C | A | a | 26 | 3 | 11 | bc | false | <90% | 1028 | ||
XR_015251 | LOC727970 | 280 | 298 | c | U | G | A | A | G | a | U | G | U | U | C | A | G | G | G | C | A | a | 26 | 3 | 11 | bc | false | <90% | 1029 | ||
XR_015702 | LOC730913 | 280 | 298 | c | U | G | A | A | G | a | U | G | U | U | C | A | G | G | G | C | A | a | 26 | 3 | 11 | bc | false | <90% | 1030 | ||
XR_015536 | LOC731599 | 281 | 299 | c | U | G | A | A | G | a | U | G | U | U | C | A | G | G | G | C | A | a | 26 | 3 | 11 | bc | false | <90% | 1031 | ||
XR_015361 | LOC728700 | 281 | 299 | c | U | G | A | A | G | a | U | G | U | U | C | A | G | G | G | C | A | a | 26 | 3 | 11 | bc | false | <90% | 1031 | ||
XR_016285 | LOC652670 | 281 | 299 | c | U | G | A | A | G | a | U | G | U | U | C | A | G | G | G | C | A | a | 26 | 3 | 11 | bc | false | <90% | 1032 | ||
XR_015984 | LOC731943 | 871 | 889 | c | U | G | A | A | G | a | U | G | U | U | C | A | G | G | G | C | A | a | 26 | 3 | 11 | bc | false | <90% | 1618 | ||
XR_015690 | LOC730993 | 870 | 888 | c | U | G | A | A | G | a | U | G | U | U | C | A | G | G | G | C | A | a | 26 | 3 | 11 | bc | false | <90% | 1620 | ||
XR_015810 | LOC731051 | 912 | 930 | c | U | G | A | A | G | a | U | G | U | U | C | A | G | G | G | C | A | a | 26 | 3 | 11 | bc | false | <90% | 1663 | ||
XR_015570 | LOC728309 | 912 | 930 | c | U | G | A | A | G | a | U | G | U | U | C | A | G | G | G | C | A | a | 26 | 3 | 11 | bc | false | <90% | 1663 | ||
XR_015819 | LOC729150 | 914 | 932 | c | U | G | A | A | G | a | U | G | U | U | C | A | G | G | G | C | A | a | 26 | 3 | 11 | bc | false | <90% | 1664 | ||
XR_015774 | LOC731260 | 914 | 932 | c | U | G | A | A | G | a | U | G | U | U | C | A | G | G | G | C | A | a | 26 | 3 | 11 | bc | false | <90% | 1664 | ||
NM_021721 | ADAM22 | 1684 | 1702 | G | U | G | A | u | G | G | U | G | U | U | C | A | G | G | G | a | A | U | 25 | 2 | 11 | c | false | <90% | 2636 | ||
NM_004194 | ADAM22 | 1684 | 1702 | G | U | G | A | u | G | G | U | G | U | U | C | A | G | G | G | a | A | U | 25 | 2 | 11 | c | false | <90% | 2744 | ||
NM_016351 | ADAM22 | 1684 | 1702 | G | U | G | A | u | G | G | U | G | U | U | C | A | G | G | G | a | A | U | 25 | 2 | 11 | c | false | <90% | 3347 | ||
NM_021722 | ADAM22 | 1684 | 1702 | G | U | G | A | u | G | G | U | G | U | U | C | A | G | G | G | a | A | U | 25 | 2 | 11 | c | false | <90% | 3393 | ||
NM_021723 | ADAM22 | 1684 | 1702 | G | U | G | A | u | G | G | U | G | U | U | C | A | G | G | G | a | A | U | 25 | 2 | 11 | c | false | <90% | 3455 | ||
NM_016118 | NUB1 | 2193 | 2211 | G | g | G | A | A | G | G | U | G | U | c | C | A | G | G | G | C | A | g | 22 | 3 | 8 | c | false | <90% | 3112 | ||
NM_004402 | DFFB | 2679 | 2697 | u | g | G | A | A | G | c | U | G | U | U | C | A | G | G | G | C | A | U | 20 | 3 | 12 | bc | false | <90% | 3034 | ||
NM_001004286 | DFFB | 2701 | 2719 | u | g | G | A | A | G | c | U | G | U | U | C | A | G | G | G | C | A | U | 20 | 3 | 12 | bc | false | <90% | 3056 | ||
NM_001004285 | DFFB | 2729 | 2747 | u | g | G | A | A | G | c | U | G | U | U | C | A | G | G | G | C | A | U | 20 | 3 | 12 | bc | false | <90% | 3084 | ||
NM_138554 | TLR4 | 3792 | 3810 | G | U | G | A | A | G | G | U | a | U | U | C | A | a | G | G | C | A | g | 15 | 3 | 8 | c | false | <90% | 5503 | ||
NM_006668 | CYP46A1 | 1339 | 1357 | G | U | G | A | A | G | G | U | G | g | U | C | A | u | G | G | C | A | a | -7 | 3 | 9 | c | false | <90% | 2138 |
3 prime UTR Seed matches
Hits to same gene : 0
Hits to other genes : 1830
View matched accessions
3 prime seed match details
Immunostimulatory motif search results
UGUGU - Judge et al. Sequence-dependent stimulation of the mammalian innate immune response by synthetic siRNA. Nat Biotechnol. 2005 Apr;23(4):457-62. (PMID: 15778705)
No Immunostimulatory motif found (UGUGU).
GUCCUUCAA - Hornung et al. Sequence-specific potent induction of IFN-alpha by short interfering RNA in plasmacytoid dendritic cells through TLR7. Nat Med. 2005 Mar;11(3):263-70. Epub 2005 Feb 20. (PMID: 15723075)
No Immunostimulatory motif found (GUCCUUCAA).
Settings used for search
Scores were calculated using scoring scheme SS1
Using AVG scoring mode.
Hit scores
Parameter | Spec. Score cut-off | Colour | Comment |
Hit to same gene | Colour | Hits to the same accession or genes with 100 percent identity (i.e. likely transcript variants). It is advisable to always check the genes hit. | |
High scoring hit | ≥80 | Colour | Hits are very likely to cause an off-target effect |
Medium scoring hit | <80¸≥40 | Colour | Hits with a small chance of causing an off target effect. |
Low scoring hit | Colour | Hits very unlikely to cause an off-target effect |
The mismatches are colour coded based on the weighting of that position.
Cut-off | Colour |
Match | Colour |
Wobble base pairs (G-U) | Colour |
Critical bases (none) | Colour |
0.6 bits | Colour |
0.4 bits | Colour |
0.2 bits | Colour |
SpecificityServer is designed to help you identify potential non-specific matches to your siRNA. It incorporates the latest information about non-specific matches (sequence-specific only).
Designed by: Alistair Chalk, Quan Du, Zicai Liang and Erik L.L. Sonnhammer