Automatic recommendation
Not OK - siRNA is not specific
9 hits were found for GCUAUUCCACCUAUGUGAA (5 guide strand hits and 4 sense strand hits.)
Hits to Guide strand
| Accession | Gene | Start | End | Details of mismatches | Spec. Score | Mismatches | Contiguous | Jackson style specificity | Hits similar gene | HSP Score | HSP % Identity | HSP length | Gene Length | ||||||||||||||||||
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | |||||||||||||
| NM_017596 | KIF21B | 505 | 523 | u | C | U | A | U | U | C | C | A | C | C | U | g | U | G | U | G | A | g | 82 | 3 | 11 | false | <90% | 9895 | |||
| NM_006635 | ZNF460 | 1446 | 1464 | a | C | U | A | U | U | C | C | A | C | C | U | A | U | G | U | c | c | u | 66 | 4 | 15 | ac | false | <90% | 3849 | ||
| NM_033100 | PCDH21 | 1523 | 1541 | a | a | U | A | U | U | C | C | A | C | C | U | A | U | G | g | G | A | c | 55 | 4 | 13 | c | false | <90% | 5337 | ||
| NM_001010934 | HGF | 821 | 839 | u | C | U | A | U | U | C | C | c | C | C | U | A | U | G | U | G | u | A | 35 | 3 | 8 | c | false | <90% | 2079 | ||
| NM_021058 | HIST1H2BJ | 156 | 174 | G | C | U | A | U | U | C | C | A | u | C | U | A | U | G | U | G | u | A | 14 | 2 | 9 | c | false | <90% | 481 | ||
Hits to Sense strand
| Accession | Gene | Start | End | Details of mismatches | Spec. Score | Mismatches | Contiguous | Jackson style specificity | Hits similar gene | HSP Score | HSP % Identity | HSP length | Gene Length | ||||||||||||||||||
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | |||||||||||||
| NM_006912 | RIT1 | 1940 | 1958 | U | U | C | A | C | A | U | A | G | G | U | G | G | A | g | U | u | u | C | 49 | 3 | 14 | c | false | <90% | 3425 | ||
| NM_020440 | PTGFRN | 5350 | 5368 | c | U | C | A | C | A | c | A | G | G | U | G | G | A | A | U | u | G | C | 8 | 3 | 9 | c | false | <90% | 6160 | ||
| NR_003366 | ANKRD20B | 16626 | 16644 | g | U | C | A | C | A | U | A | G | u | U | G | G | A | A | U | A | a | u | 7 | 4 | 8 | c | false | <90% | 96524 | ||
| NM_000245 | MET | 5721 | 5739 | U | U | C | A | C | c | U | A | G | a | U | G | G | A | A | U | A | G | C | -46 | 2 | 9 | bc | false | <90% | 6641 | ||
3 prime UTR Seed matches
Hits to same gene : 0
Hits to other genes : 1034
View matched accessions
3 prime seed match details
Immunostimulatory motif search results
UGUGU - Judge et al. Sequence-dependent stimulation of the mammalian innate immune response by synthetic siRNA. Nat Biotechnol. 2005 Apr;23(4):457-62. (PMID: 15778705)
No Immunostimulatory motif found (UGUGU).
GUCCUUCAA - Hornung et al. Sequence-specific potent induction of IFN-alpha by short interfering RNA in plasmacytoid dendritic cells through TLR7. Nat Med. 2005 Mar;11(3):263-70. Epub 2005 Feb 20. (PMID: 15723075)
No Immunostimulatory motif found (GUCCUUCAA).
Settings used for search
Scores were calculated using scoring scheme SS1
Using AVG scoring mode.
Hit scores
| Parameter | Spec. Score cut-off | Colour | Comment |
| Hit to same gene | Colour | Hits to the same accession or genes with 100 percent identity (i.e. likely transcript variants). It is advisable to always check the genes hit. | |
| High scoring hit | ≥80 | Colour | Hits are very likely to cause an off-target effect |
| Medium scoring hit | <80¸≥40 | Colour | Hits with a small chance of causing an off target effect. |
| Low scoring hit | Colour | Hits very unlikely to cause an off-target effect |
The mismatches are colour coded based on the weighting of that position.
| Cut-off | Colour |
| Match | Colour |
| Wobble base pairs (G-U) | Colour |
| Critical bases (none) | Colour |
| 0.6 bits | Colour |
| 0.4 bits | Colour |
| 0.2 bits | Colour |
SpecificityServer is designed to help you identify potential non-specific matches to your siRNA. It incorporates the latest information about non-specific matches (sequence-specific only).
Designed by: Alistair Chalk, Quan Du, Zicai Liang and Erik L.L. Sonnhammer