Predicted Off-targets for siRNA "guacagguaagaugcucau" Using siRNA SpecificityServer

Specificity Server [Result page v1.0]


SpecificityServer - An siRNA design program for determining siRNA specificity

Automatic recommendation
OK - siRNA is specific for both sense and antisense (guide) strands

8 hits were found for GUACAGGUAAGAUGCUCAU (1 guide strand hits and 7 sense strand hits.)

Hits to Guide strand

AccessionGeneStartEndDetails of mismatchesSpec.
Score
MismatchesContiguousJackson style
specificity
Hits similar
gene
HSP
Score
HSP %
Identity
HSP lengthGene Length
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19
NM_019072 SGTB 1794 1812 GUAaAGGUAgGAUGCUCAg-24 3 8 bc false   <90%   5453


Hits to Sense strand








AccessionGeneStartEndDetails of mismatchesSpec.
Score
MismatchesContiguousJackson style
specificity
Hits similar
gene
HSP
Score
HSP %
Identity
HSP lengthGene Length
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19
NM_019555 ARHGEF3 1741 1759 gccuGCAUCUUACCUGUAC30 4 15 bc false   <90%   3561
NM_001080519 BAHCC1 9000 9018 gUGAGCAUCUcACCUGUgu29 4 9 c false   <90%   10708
XM_001134463 BAHCC1 9117 9135 gUGAGCAUCUcACCUGUgu29 4 9 c false   <90%   10825
NM_000603 NOS3 4210 4228 AgGAGuAUCUUACCUGUAa20 3 12 bc false   <90%   4341
NM_001025109 CD34 2195 2213 cUGAGCcUCUcACCUGUAC-29 3 8 c false   <90%   2621
NM_001773 CD34 2390 2408 cUGAGCcUCUcACCUGUAC-29 3 8 c false   <90%   2816
NM_002957 RXRA 3389 3407 uUGAGCcUCaUACCUGUAC-51 3 9 bc false   <90%   5449


3 prime UTR Seed matches

Hits to same gene : 0
Hits to other genes : 1161
View matched accessions

3 prime seed match details

AccessionGene nameHits to this gene
NM_001079906ZNF3312
NM_018555ZNF3312
NM_001079907ZNF3312
NM_024345WDR322
NM_020119ZC3HAV12
XM_376795FLJ458722
XM_940037FLJ458722
NM_145256LRRC252
NM_013255MKLN12
NM_133460ZNF4182
NM_145206VTI1A2
NM_003421ZNF37A2
NM_001007094ZNF37A2
NM_018451CENPJ2
NM_002417MKI672
NM_000314PTEN2
NM_024798SNX222
NM_0209112
NM_024989PGAP12
NM_003298NR2C22
NM_152222TNFRSF19L2
NM_032871TNFRSF19L2
NM_139157ST52
NM_005418ST52
NM_213618ST52
NM_007153ZNF2082
NM_017919STX172
NM_177452TRAPPC6B2
NM_001079537TRAPPC6B2
NM_001012763GNRHR2
NM_000406GNRHR2
NM_032869NUDCD12
NM_138959VANGL12
NM_003972BTAF12
NM_002015FOXO1A2
NM_001966EHHADH2
NM_001008397LOC4938692
NM_006047RBM122
NM_152838RBM122
NM_145261DNAJC192
NM_032373PCGF52
NM_138994CNTNAP42
NM_033401CNTNAP42
NM_001083625ANKRD122
NM_015208ANKRD122
NM_012334MYO102
NM_004873BAG52
NM_001015049BAG52
NM_001015048BAG52
NM_013231FLRT22
XM_379273FLJ141862
XM_001130364LOC7284172
XM_001130184LOC7308432
NM_0011008292
NM_153487MDGA12
NM_000396CTSK2
NM_005551KLK22
NM_001002231KLK22
NM_001001665FLJ160082
NM_001025780FAM108B12
NM_022745ATPAF12
NM_001042546ATPAF12
NM_004742MAGI12
XM_001132013ANKH2
NM_017575SMG62
NM_000136FANCC2
NM_014333CADM12
NM_0010985172
NM_014911AAK12
NM_152263TPM32
NM_022757CCDC142
NM_018159NUDT112
NM_016205PDGFC1
NM_004171SLC1A21
NM_001030055ARHGAP51
NM_001173ARHGAP51
NM_003799RNMT1
NM_001423EMP11
NM_001024372BAALC1
NM_024812BAALC1
NM_133638ADAMTS191
NM_018036C14orf1031
NM_015286DMN1
NM_145728DMN1
NM_001040105MUC171
NM_080670SLC35A41
NM_080927DCBLD21
NM_001010867C1orf691
NM_001003954ANXA131
NM_004306ANXA131
NM_033468ZNF2571
NM_001018075NR3C11
NM_001018077NR3C11
NM_001018076NR3C11
NM_001018074NR3C11
NM_001024094NR3C11
NM_000176NR3C11
NM_003348UBE2N1
XM_001133042PDZRN31
NM_015009PDZRN31
NM_002725PRELP1
NM_201348PRELP1
NM_018717MAML31
NM_171982TRIM351
NM_197970BOLL1
NM_033030BOLL1
NM_006418OLFM41
NM_013411AK21
NM_0011026081
NM_003017SFRS31
NM_145333MAP3K71
NM_145332MAP3K71
NM_015396ARMC81
NM_022492TTC311
NM_057178RFFL1
NM_001017368RFFL1
NM_005233EPHA31
NM_001080428ODZ21
XM_945786ODZ21
XM_945788ODZ21
XM_942208ODZ21
XM_931456ODZ21
NM_024915GRHL21
NM_018111FLJ104901
NM_001006620MAPKAP11
NM_001006619MAPKAP11
NM_001006621MAPKAP11
NM_024117MAPKAP11
NM_001006617MAPKAP11
NM_003204NFE2L11
NM_014922NLRP11
NM_033006NLRP11
NM_033007NLRP11
NM_033004NLRP11
NM_000544TAP21
NM_175607CNTN41
NM_175612CNTN41
NM_207491MGC486281
NM_016930STX181
XM_001129009LOC7282221
NM_003775EDG61
NM_003642HAT11
NM_001033085HAT11
NM_000912OPRK11
XM_001129946LOC7303961
XM_001130706LOC7295831
XM_001131223LOC7304051
XM_001131133LOC7318851
NM_173667FLJ375431
NM_138991BACE21
NM_138992BACE21
NM_012105BACE21
NM_012219MRAS1
NM_0010850491
NM_015174KIAA10861
NM_002213ITGB51
XM_001129224ITGB51
NM_177996EPB41L11
NM_012156EPB41L11
NM_003012SFRP11
NM_005872BCAS21
NM_013443ST6GALNAC61
NM_001004432LINGO41
NM_206933USH2A1
NM_182515ZNF7141
XM_929032LOC6460711
XM_001125928LOC2838041
XM_001126277LOC6489131
NM_018254RCOR31
NM_000461THRB1
NM_145804ABTB21
NM_033394TANC11
NM_018584CAMK2N11
NM_017556FBLIM11
NM_001024216FBLIM11
NM_178585WDFY31
NM_014991WDFY31
NM_004823KCNK61
NM_022080NAPB1
NM_030583MATN21
NM_002380MATN21
NM_012238SIRT11
NM_152376UBXD31
NM_004947DOCK31
NM_020844KIAA14561
NM_0010996771
NM_177968PPM1B1
NM_002609PDGFRB1
NM_014930ZNF5101
NM_032902PPP1R16A1
NM_015950MRPL21
NM_015156RCOR11
NM_001039960SLC4A81
NM_004791ITGBL11
NM_021964ZNF1481
NM_001031681CTNS1
NM_004937CTNS1
NM_001992F2R1
NM_016396CTDSPL21
NM_145655GCNT21
NM_145649GCNT21
NM_001491GCNT21
NM_013381TRHDE1
NM_001004304ZNF7401
NM_182663RASSF51
NM_182664RASSF51
NM_182665RASSF51
NM_018194HHAT1
NM_018351FGD61
NM_020159SMARCAD11
NM_173491LSM111
NM_152309PIK3AP11
NM_024667VPS37B1
NM_024626VTCN11
NM_198595AFAP11
NM_021638AFAP11
NM_006716DBF41
NM_006778TRIM101
NM_016374ARID4B1
NM_031371ARID4B1
XM_001130886FLJ370341
XM_001127207FLJ370341
NM_001033521CSTF11
NM_001033522CSTF11
NM_001324CSTF11
NM_004894C14orf21
XM_001128799MAP3K21
NM_024795TM4SF201
NM_022898BCL11B1
NM_138576BCL11B1
NM_001083591CUGBP21
NM_001025077CUGBP21
NM_006561CUGBP21
NM_001025076CUGBP21
NM_002822TWF11
NM_015266SLC9A81
XM_943391LOC3399701
XM_291095LOC3399701
NM_001018090Gcom11
NM_001018102GRINL1A1
NM_015532GRINL1A1
NM_001018091Gcom11
NM_020774MIB11
NM_0245241
NM_030940ISCA11
NM_0011023961
NM_025073SIKE1
NM_052926PNMA51
NM_0011031511
NM_0011031501
NM_020708SLC12A51
NM_031911C1QTNF71
NM_138479C1orf2131
NM_032482DOT1L1
NM_004348RUNX21
NM_001015051RUNX21
NM_001024630RUNX21
NM_0204591
NM_004428EFNA11
NM_182685EFNA11
NM_024316LENG11
NM_005440RND21
XM_379228LOC4010931
XM_944985LOC4010931
NM_012249RHOQ1
NM_147128ZNRF21
NM_032470TNXB1
NM_001002919LOC2850161
NM_020184CNNM41
NM_015937PIGT1
NM_003307TRPM21
NM_002914RFC21
NM_181471RFC21
NM_001007237IGSF31
NM_001542IGSF31
NM_144586LYPD11
NM_001077427LYPD11
NM_002221ITPKB1
XM_001130409LOC7294881
NM_207363NAP51
NM_207481NAP51
NM_001039796FLJ357761
NM_006479RAD51AP11
NM_001040441ZBTB81
XM_001124597LOC7304111
NM_080819GPR781
NM_178448C9orf1401
NM_0011004231
NM_0011004221
NM_0011004241
NM_015535LOC260101
NM_006574CSPG51
NM_021163RBAK1
NM_0011003981
NM_015085GARNL41
NM_032829C12orf341
NM_001003676C11orf491
XM_939083TTC281
XM_929318TTC281
NM_144973MGC240391
NM_018360CXorf151
NM_178844NLRC31
NM_017432PTOV11
NM_018379FAM63A1
NM_058246DNAJB61
NM_001080524LOC6461741
XM_940146LOC6510351
NM_015076CDC2L61
NM_033161SURF41
NM_006581FUT91
NM_020804PACSIN11
NM_133443GPT21
NM_007030TPPP1
NM_001010846SHE1
NM_177988MRPL471
NM_020409MRPL471
NM_005207CRKL1
NM_078487CDKN2B1
NM_006553SLMO11
NM_198562C3orf621
NM_016252BIRC61
NM_138703MAGEE21
NM_178140PDZD21
NM_019095CRLS11
NM_005665EVI51
NM_0011112831
NM_000618IGF11
NM_0011112841
NM_003846PEX11B1
NM_023934FUNDC21
NM_001077484SLC38A11
NM_030674SLC38A11
NM_032873STS-11
NM_003326TNFSF41
NM_000791DHFR1
NM_176815DHFRL11
NM_018318CCDC911
NM_0011024451
NM_005613RGS41
NM_003578SOAT21
NM_018700TRIM361
NM_005385NKTR1
NM_001025108AFF31
NM_002285AFF31
NM_005749TOB11
NM_015253WSCD11
NM_020657ZNF3041
NM_002657PLAGL21
NM_006851GLIPR11
NM_022719DGCR141
NM_007350PHLDA11
NM_014932NLGN11
NM_005188CBL1
NM_014680KIAA01001
NM_004273CHST31
NM_014341MTCH11
NM_018689KIAA11991
NM_003728UNC5C1
NM_014916LMTK21
NM_000133F91
NM_006667PGRMC11
NM_016542MST41
NM_001042452MST41
NM_001042453MST41
NM_014296CAPN71
NM_000336SCNN1B1
NM_181523PIK3R11
NM_181504PIK3R11
NM_181524PIK3R11
XM_938418LOC6467621
NM_001039772LOC6439231
NM_000734CD2471
NM_198053CD2471
NM_212554LOC3998181
NM_002508NID11
NM_002497NEK21
NM_001017978LOC2034131
NM_178435LCE3E1
NM_183413FBXO441
NM_033182FBXO441
NM_001014765FBXO441
NM_183412FBXO441
NM_001040272C9orf941
NM_002009FGF71
XM_933333LOC6534491
NM_013328PYCR21
NM_005962MXI11
NM_001008541MXI11
NM_130439MXI11
NM_001017523BTBD111
NM_001018072BTBD111
NM_021912GABRB31
NM_000814GABRB31
NM_203329CD591
NM_203331CD591
NM_203330CD591
NM_000611CD591
NM_022130GOLPH31
NM_194248OTOF1
NM_004802OTOF1
NM_194323OTOF1
NM_194322OTOF1
NM_000428LTBP21
NM_001029998SLC10A71
NM_144590ANKRD221
NM_032933C18orf451
NM_181719TMCO41
NM_194071CREB3L21
NM_130768ASZ11
NM_182916TRNT11
NM_032932RAB11FIP41
NM_175876EXOC81
NM_014732KIAA05131
NM_181443BTBD31
NM_014962BTBD31
NM_005957MTHFR1
NM_182607VSIG11
NM_173664ARL101
NM_005010NRCAM1
NM_001037133NRCAM1
NM_001037132NRCAM1
NM_003126SPTA11
NM_175862CD861
NM_006889CD861
XM_001133101LOC7289171
NM_001080422FAM108A21
NM_031213FAM108A11
XM_933830FAM108A21
NM_006566CD2261
NM_016577RAB6B1
NM_014190ADD11
NM_001119ADD11
NM_014189ADD11
NM_176801ADD11
NM_016080GLOD41
NM_0190041
NM_001032290PSRC11
NM_001032291PSRC11
NM_032636PSRC11
NM_001005290PSRC11
NM_020338ZMIZ11
NM_000261MYOC1
NM_002594PCSK21
NM_012393PFAS1
NM_015294TRIM371
NM_003943GENX-34141
NM_015256ACSL61
NM_001009185ACSL61
NM_145166ZBTB471
NM_198830ACLY1
NM_001096ACLY1
NM_018189DPPA41
XM_941892LOC6542441
XM_930446LOC6536981
NM_170665ATP2A21
NM_024830AYTL21
NM_022824FBXL171
NM_013446MKRN11
NM_014432IL20RA1
NM_015321CRTC11
NM_0010984821
NM_003469SCG21
NM_025152NUBPL1
NM_032966BLR11
NM_001716BLR11
NM_006517SLC16A21
NM_152330FRMD61
NM_001042481FRMD61
NM_001025079CD471
NM_001777CD471
NM_198793CD471
NM_031419NFKBIZ1
NM_001005474NFKBIZ1
NM_000342SLC4A11
NM_020418PCBP41
NM_033008PCBP41
NM_033009PCBP41
NM_033010PCBP41
NM_017482ADD21
NM_017484ADD21
XM_001128311LOC7314491
XM_001129750LOC7289991
NM_182811PLCG11
NM_002660PLCG11
NM_004114FGF131
NM_033642FGF131
NM_019043APBB1IP1
NM_198484ZNF6211
NM_0010984141
NM_198586NHLRC11
NM_173854SLC41A11
NM_006060IKZF11
NM_152547BTNL91
NM_001012509SLC45A21
NM_138768MYEOV1
NM_017924C14orf1191
NM_000831GRIK31
NM_017856GEMIN81
NM_001042479GEMIN81
NM_001042480GEMIN81
NM_003161RPS6KB11
NM_001991EZH11
NM_003395WNT9A1
NM_0010996701
NM_0010996721
NM_0010996711
NM_0010996731
NM_001034852SMOC11
NM_022137SMOC11
NM_002119HLA-DOA1
NM_005853IRX51
NM_138428C1orf2121
NM_003173SUV39H11
NM_003908EIF2S21
NM_0010109061
XM_001125737LOC7302751
NM_001008215MGC521101
NM_175736FMNL31
NM_198900FMNL31
NM_145010C10orf631
NM_138967SCAMP51
NM_014368LHX61
NM_199160LHX61
NM_030761WNT41
NM_006393NEBL1
NM_017437CPSF21
NM_001025266LOC2853821
XM_933614LOC6464341
NM_001008537KIAA20221
NM_016606REEP21
XM_065166KIAA19571
XM_939576KIAA19571
NM_006522WNT61
NM_0150161
NM_001031702SEMA5B1
NM_032701SUV420H21
NM_133330WHSC11
NM_001042424WHSC11
NM_133331WHSC11
NM_133335WHSC11
NM_080539COLQ1
NM_080538COLQ1
NM_080544COLQ1
NM_005677COLQ1
NM_080541COLQ1
NM_022340ZFYVE201
NM_030820COL21A11
NM_016647C8orf551
NM_016298FBXO401
NM_139278LGI31
NM_019042PUS71
NM_016339RAPGEFL11
NM_054012ASS11
NM_000050ASS11
NM_003496TRRAP1
NM_021984GABRE1
NM_021987GABRE1
NM_004961GABRE1
NM_021990GABRE1
NM_005813PRKD31
NM_0011003891
NM_001043318C14orf431
NM_194278C14orf431
NM_024295DERL11
NM_005018PDCD11
XM_934287LOC1448171
XM_945225LOC1448171
NM_016532SKIP1
NM_130766SKIP1
NM_020750XPO51
NM_024627C22orf291
NM_014430CIDEB1
NM_001701BAAT1
NM_018963BRWD11
NM_031473IFT811
NM_0247761
NM_182493MLCK1
NM_022143LRRC41
NM_015026MON21
NM_153645NUP501
NM_007172NUP501
NM_006591POLD31
NM_172000TEDDM11
XM_001125827LOC7278201
NM_016316REV11
NM_001037872REV11
NM_0321731
NM_198381ELF51
NM_001422ELF51
NM_007050PTPRT1
NM_133170PTPRT1
NM_0010839621
NM_003199TCF41
NM_005439MLF21
NM_006245PPP2R5D1
NM_180976PPP2R5D1
NM_180977PPP2R5D1
NM_030958SLCO5A11
NM_006108SPON11
NM_001329CTBP21
NM_0010839141
NM_022802CTBP21
NM_006377UNC13B1
NM_018953HOXC51
NM_016621PHF21A1
NM_0011018021
NM_001561TNFRSF91
NM_000276OCRL1
NM_001587OCRL1
NM_000259MYO5A1
NM_178468FAM83C1
NM_015347RIMBP21
NM_005785RNF411
NM_194359RNF411
NM_194358RNF411
NM_032626RBBP61
NM_004274AKAP61
NM_032980DTNA1
NM_001390DTNA1
NM_032975DTNA1
NM_032160DSEL1
NM_020894KIAA15301
XM_001131744LOC7320601
XM_001126476LOC7280731
NM_194247HNRPA31
NM_001009934DNASE1L11
NM_001009932DNASE1L11
NM_006730DNASE1L11
NM_001009933DNASE1L11
NM_173214NFAT51
NM_006599NFAT51
NM_138714NFAT51
NM_0011131781
NM_138713NFAT51
XM_001127175LOC7283201
NM_018388MBNL31
NM_080491GAB21
NM_012296GAB21
NM_199368TRPC4AP1
NM_015638TRPC4AP1
NM_001012393OPCML1
NM_002545OPCML1
NM_022845CBFB1
NM_001755CBFB1
NM_152624DCP21
NM_006493CLN51
NM_015316PPP1R13B1
NM_007174CIT1
NM_001007546FLJ468381
NM_134265WSB11
NM_015626WSB11
XM_001134327LOC7323241
XM_001134208LOC7299751
NM_014240LIMD11
NM_0010992721
NM_152733BTBD91
NM_0528931
NM_152404UGT3A11
NM_000926PGR1
NM_001077497PRR31
NM_025263PRR31
NM_206996SPAG171
NM_004176SREBF11
NM_001005291SREBF11
NM_004308ARHGAP11
NM_001042633SNX211
NM_033421SNX211
NM_001042632SNX211
NM_199050C21orf251
NM_015500C21orf251
XM_032945C21orf251
NM_000925PDHB1
NM_018444PPM2C1
NM_152622MIER31
NM_014358CLEC4E1
NM_173598KSR21
NM_022720DGCR81
NM_005011NRF11
NM_001040110NRF11
NM_001001412FAM26C1
NM_015471NSL11
NM_001042549NSL11
NM_001080530LOC920171
NM_020899ZBTB41
NM_182578THEM51
NM_033011PLAT1
NM_000930PLAT1
NM_001177ARL11
NM_001012339DNAJA51
NM_194283DNAJA51
NM_014848SV2B1
NM_001079878CNGA31
NM_001298CNGA31
NM_152914MGC338941
NM_001026213CYP11B11
NM_000497CYP11B11
NM_178519C17orf551
NM_213605ZNF5171
NM_001448GPC41
NM_017637BNC21
NM_030799YIPF51
NM_134426SLC26A61
NM_134263SLC26A61
NM_001040454SLC26A61
NM_022911SLC26A61
NM_016174CEECAM11
NM_001024843TNRC6B1
NM_015088TNRC6B1
NM_022744C16orf581
NM_201264NRP21
NM_015187KIAA07461
NM_001077243GRIA41
NM_000829GRIA41
XM_001132359LOC7300911
XM_001132046LOC7321641
NM_194317LYPD61
NM_003484HMGA21
NM_001025369VEGFA1
NM_001033756VEGFA1
NM_001025370VEGFA1
NM_001025368VEGFA1
NM_001025366VEGFA1
NM_001025367VEGFA1
NM_003376VEGFA1
NM_021095SLC5A61
NM_012297G3BP21
NM_203504G3BP21
NM_203505G3BP21
NM_018061PRPF38B1
NM_003679KMO1
NM_000217KCNA11
NM_006754SYPL11
NM_182715SYPL11
NM_152673MUC201
NM_0010985161
NM_018361AGPAT51
NM_001978EPB491
NM_003028SHB1
NM_002835PTPN121
NM_031435THAP21
NM_018381FLJ112861
NM_001029883FLJ349311
NM_000268NF21
NM_016418NF21
NM_181832NF21
NM_181828NF21
NM_181829NF21
NM_181833NF21
NM_181830NF21
NM_030641APOL61
NM_021976RXRB1
NM_000742CHRNA21
NM_001631ALPI1
NM_005125CCS1
XM_001129630CCS1
NM_080797DIDO11
NM_0010994091
NM_015456COBRA11
XM_001133009COBRA11
NM_000234LIG11
NM_001037494DYNLL11
NM_001037495DYNLL11
NM_003746DYNLL11
NM_004411DYNC1I11
NM_0209101
NM_013364PNMA31
NM_144635FAM131A1
NM_005491CXorf61
XM_060535LOC1275451
XM_940692LOC1275451
NM_014734KIAA02471
XM_001132650LOC1494781
XM_001128950LOC1494781
NM_033656BRWD11
NM_015995KLF131
NM_173618CCDC951
XM_001134215PDPR1
NM_006693CPSF41
NM_001081559CPSF41
NM_000219KCNE11
NM_017789SEMA4C1
NM_199487C20orf441
NM_018244C20orf441
NM_005994TBX21
NM_004840ARHGEF61
NM_004569PIGH1
NM_175630DNMT3A1
XM_371534LOC3890001
NM_139173NHEDC11
XM_001125983LOC7278601
XM_925973LOC6530971
NM_032508TMEM185A1
NM_001031690FAM131B1
NM_014690FAM131B1
NM_002648PIM11
NM_016135ETV71
NM_005238ETS11
NM_198451FOXR21
NM_173653SLC9A91
NM_144688FLJ326581
NM_003032ST6GAL11
NM_173217ST6GAL11
NM_173216ST6GAL11
NM_000951PRRG21
XM_001134358LOC7322731
NM_003107SOX41
NM_001856COL16A11
NM_002579PALM1
NM_001040134PALM1
NM_003131SRF1
NM_014665LRRC141
NM_138572TBN1
XM_925795LOC6530511
NM_014235UBL4A1
NM_173689CRB21
XM_499022LOC4411241
NM_006774INMT1
NM_020850RANBP101
NM_014424HSPB71
NM_007368RASA31
NM_001080410FBXO411
XM_001133261FBXO411
NM_0207411
NM_153380ZNF411
NM_007130ZNF411
NM_000843GRM61
NM_014761KIAA01741
XM_001129232LOC7310491
NM_014501UBE2S1
NM_032447FBN31
NM_017643MBTD11
NM_001012729DUXA1
NM_130847AMOTL11
NM_032862TIGD51
NM_015172BAT2D11
NM_003077SMARCD21
NM_0010984261
NM_002160TNC1
NM_152441FBXL141
NM_015299KIAA03231
NM_005158ABL21
NM_007314ABL21
NM_0011001081
NM_000838GRM11
NM_201651SLC28A11
NM_000602SERPINE11
NM_014438IL1F81
NM_052867NALCN1
NM_003083SNAPC21
NM_014233UBTF1
NM_001076683UBTF1
NM_001076684UBTF1
NM_173808NEGR11
NM_015900PLA1A1
NM_021072HCN11
NM_006464TGOLN21
NM_003275TMOD11
NM_020429SMURF11
NM_181349SMURF11
NM_006933SLC5A31
NM_172239REXO1L11
NM_020477ANK11
NM_020475ANK11
NM_000037ANK11
NM_020476ANK11
NM_020478ANK11
NM_020480ANK11
NM_153695ZNF3671
NM_000564IL5RA1
NM_175726IL5RA1
NM_014810CEP3501
NM_021064HIST1H2AG1
NM_000262NAGA1
NM_018096NLE11
NM_001014445NLE11
NM_174976ZDHHC221
NM_018117BRWD21
NM_005324H3F3B1
NM_0010996821
NM_024859MAGIX1
NM_0010996801
NM_0010996811
NM_1735951
NM_0010988131
NM_0151461
NM_0010988121
NM_012330MYST41
NM_198264C1orf21
NM_006589C1orf21
NM_014071NCOA61
NM_030961TRIM561
NM_178554KY1
NM_182577C19orf191
NM_024598C16orf571
XM_001129337LOC7313581
XM_001131925LOC7299711
NM_032012C9orf51
NM_0010997341
NM_020699GATAD2B1
NM_0153061
NM_012223MYO1B1
NM_004468FHL31
NM_152757FLJ303131
NM_004430EGR31
XM_001130047EGR31
NM_003999OSMR1
NM_002779PSD1
NM_031947SLC25A21
NM_013345GPR1321
NM_001455FOXO3A1
NM_201559FOXO3A1
NM_007010DDX521
NM_152300DDX521
XM_001134431ALS2CR161
XM_001134454ALS2CR161
NM_001039905FLJ385961
NM_006924SFRS11
NM_001078166SFRS11
NM_203390RBM12B1
NM_005895GOLGA31
NM_022764MTHFSD1
NM_182597FLJ395751
NM_0011113081
NM_0011113091
NM_0011113071
NM_006202PDE4A1
NM_181501ITGA11
NM_006056NMUR11
NM_017772TBC1D22B1
NM_203451LOC4001201
NM_002513NME31
NM_201252AFAR31
NM_016485VTA11
NM_001546ID41
NM_004432ELAVL21
NM_001008539SLC7A21
NM_003046SLC7A21
NM_020428SLC44A21
NM_001079685KIAA11911
NM_020444KIAA11911
NM_001079684KIAA11911
NM_174899FBXO361
NM_016230CYB5R41
NM_032189ATP11A1
NM_015205ATP11A1
NM_001460FMO21
NM_000579CCR51
NM_0011001681
NM_0011055361
NM_018650MARK11
NM_001003652SMAD21
NM_005901SMAD21
NM_000332ATXN11
NM_007203PALM2-AKAP21
NM_147150PALM2-AKAP21
NM_001004065AKAP21
NM_054027ANKH1
NM_080632UPF3B1
NM_023010UPF3B1
NM_006282STK41
NM_001257CDH131
NM_014586HUNK1
NM_021132PPP3CB1
NM_005898CAPRIN11
NM_152850PIGO1
NM_032634PIGO1
NM_002024FMR11
NM_012287CENTB21
NM_001001420SMAD51
NM_001001419SMAD51
NM_005903SMAD51
NM_0010988331
NM_0202181
NM_005313PDIA31
NM_020914RNF2131
NM_032421CLIP21
NM_003388CLIP21
NM_014779TSC22D21
NM_005329HAS31
NM_001081573GAB31
NM_080612GAB31
NM_173470TMEM321
NM_031448C19orf121
NM_001031726C19orf121
NM_001211BUB1B1
NM_052904KLHL321
NM_032175UTP151
NM_144633KCNH81
NM_002920RFX41
NM_139279MCFD21
NM_0010853751
NM_001609ACADSB1
NM_001776ENTPD11
NM_0010981751
NM_153607LOC1532221
NM_018085IPO91
NM_198087ZNF2001
NM_198088ZNF2001
NM_003454ZNF2001
NM_005215DCC1
NM_014048MKL21
NM_015202KIAA05561
NM_004815ARHGAP291
NM_004628XPC1
NM_015058KIAA05641
NM_178129P2RY81
NM_005484PARP21
NM_001042618PARP21
NM_030806C1orf211
NM_033285TP53INP11
NM_021067GINS11
NM_198881TBC1D8B1
NM_139135ARID1A1
NM_006015ARID1A1
NM_152744SDK11
NM_004172SLC1A31
NM_001040202PAQR31
NM_004513IL161
NM_172217IL161
NM_017491WDR11
NM_005112WDR11
NM_133334WHSC11
NM_000138FBN11
NM_001040653ZXDC1
NM_152605ZNF7811
NM_022048CSNK1G11
NM_020375C12orf51
XM_001128475LOC2851911
NM_002765PRPS21
NM_001039091PRPS21
NM_144767AKAP131
NM_006738AKAP131
NM_007200AKAP131
NM_022901LRRC191
NM_018999KIAA11281
NM_004464FGF51
NM_033143FGF51
NM_001003937TSPYL61
NM_015901NUDT131
NM_002126HLF1
NM_014337PPIL21
NM_148176PPIL21
NM_148175PPIL21
NM_021946BCORL11
NM_014763MRPL191
NM_0152071
NM_052918SORCS11
NM_001013031SORCS11
NM_003918GYG21
NM_001079855GYG21
NM_014948UBOX51
NM_199415UBOX51
NM_004225MFHAS11
NM_0010976401
NM_0010976391
NM_0010976411
NM_000149FUT31
NM_020826SYT131
NM_004375COX111
NM_024086METT10D1
NM_024827HDAC111
NM_000719CACNA1C1
NM_080836STK351
NM_006306SMC1A1
NM_014859KIAA06721
NM_015317PUM21
NM_133445GRIN3A1
NM_020831MKL11
NM_006359SLC9A61
NM_001042537SLC9A61
NM_001232CASQ21
NM_213589RAPH11
NM_0208461
NM_020967NCOA51
NM_207171POGZ1
NM_015100POGZ1
NM_001527HDAC21
NM_003052SLC34A11
NM_002251KCNS11
NM_015190DNAJC91
NM_014766SCRN11
NM_198836ACACA1
NM_198837ACACA1
NM_198834ACACA1
NM_198838ACACA1
NM_198839ACACA1
NM_006314CNKSR11
NM_015529MOXD11
NM_018420SLC22A151
NM_002225IVD1
NM_000459TEK1
NM_0011110391
NM_152736ZNF1871
NM_001023560ZNF1871
NM_139169TRUB11
NM_138782FCHO21
NM_182666UBE2E11
NM_003341UBE2E11
XM_932931LOC6454851
XM_944437LOC6454851
NM_138423CASC41
NM_177974CASC41
NM_022912REEP11
NM_199169TMEPAI1
NM_199170TMEPAI1
NM_199171TMEPAI1
NM_020182TMEPAI1
NM_182641BPTF1
NM_004459BPTF1
NM_001003845SP51
XM_001134034LOC7297441
NM_013399C16orf51
NM_003292TPR1
NM_001103ACTN21
NM_022730COPS7B1
NM_014898ZFP301
NM_006033LIPG1
NM_016275SELT1
NM_007345ZNF2361
NM_0156561
NM_033118MYLK21
NM_021117CRY21
NM_001035534FAM129B1
NM_022833FAM129B1
NM_001012643LOC3393441
XM_378661C17orf511
XM_944416C17orf511
NM_000201ICAM11
NM_006938SNRPD11
NM_016131RAB101
NM_016090RBM71
NM_005073SLC15A11
NM_001310CREBL21
NM_015421TMEM1861
NM_006912RIT11
NM_001895CSNK2A11
NM_177560CSNK2A11
NM_177559CSNK2A11
NM_015458MTMR91
NM_000597IGFBP21
NM_0010087811
XM_936538FAT31
XM_926199FAT31
NM_030919FAM83D1
NM_006080SEMA3A1
NM_005917MDH11
NM_022728NEUROD61
NM_016651DACT11
NM_001079520DACT11
NM_003153STAT61
NM_003772JRKL1
NM_032682FOXP11
NM_006731FCMD1
NM_001079802FCMD1
NM_001009899KIAA20181
NM_016468C14orf1121
NM_006827TMED101
NM_032376TMEM1011
NM_012345NUFIP11
XM_001134463BAHCC11
NM_001080519BAHCC11

Immunostimulatory motif search results


UGUGU - Judge et al. Sequence-dependent stimulation of the mammalian innate immune response by synthetic siRNA. Nat Biotechnol. 2005 Apr;23(4):457-62. (PMID: 15778705)
No Immunostimulatory motif found (UGUGU).

GUCCUUCAA - Hornung et al. Sequence-specific potent induction of IFN-alpha by short interfering RNA in plasmacytoid dendritic cells through TLR7. Nat Med. 2005 Mar;11(3):263-70. Epub 2005 Feb 20. (PMID: 15723075)
No Immunostimulatory motif found (GUCCUUCAA).



Settings used for search

Scores were calculated using scoring scheme SS1
Using AVG scoring mode.

Hit scores

ParameterSpec. Score cut-offColourComment
Hit to same geneColourHits to the same accession or genes with 100 percent identity (i.e. likely transcript variants). It is advisable to always check the genes hit.
High scoring hit≥80ColourHits are very likely to cause an off-target effect
Medium scoring hit<80¸≥40ColourHits with a small chance of causing an off target effect.
Low scoring hitColourHits very unlikely to cause an off-target effect

The mismatches are colour coded based on the weighting of that position.

Cut-offColour
MatchColour
Wobble base pairs (G-U)Colour
Critical bases (none)Colour
0.6 bitsColour
0.4 bitsColour
0.2 bitsColour

About SpecificityServer

SpecificityServer is designed to help you identify potential non-specific matches to your siRNA. It incorporates the latest information about non-specific matches (sequence-specific only).

Designed by: Alistair Chalk, Quan Du, Zicai Liang and Erik L.L. Sonnhammer


For more details about the algorithm "siRNA specificity searching incorporating mismatch tolerance data" Bioinformatics (2008) 24 (10): 1316-1317