Automatic recommendation
OK - siRNA is specific for both sense and antisense (guide) strands
52 hits were found for GAGUUCCUGAUCUUCUGGU (15 guide strand hits and 37 sense strand hits.)
Hits to Guide strand
| Accession | Gene | Start | End | Details of mismatches | Spec. Score | Mismatches | Contiguous | Jackson style specificity | Hits similar gene | HSP Score | HSP % Identity | HSP length | Gene Length | ||||||||||||||||||
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | |||||||||||||
| XM_927413 | LOC643412 | 445 | 463 | a | A | G | U | U | C | C | U | G | A | U | C | U | U | g | U | G | G | a | 68 | 3 | 13 | c | false | <90% | 961 | ||
| XM_937493 | LOC647969 | 445 | 463 | a | A | G | U | U | C | C | U | G | A | U | C | U | U | g | U | G | G | a | 68 | 3 | 13 | c | false | <90% | 961 | ||
| NM_001080407 | GLB1L3 | 379 | 397 | a | A | G | U | U | C | C | U | G | A | U | C | U | U | C | g | G | G | g | 66 | 3 | 14 | c | false | <90% | 2706 | ||
| XM_001131501 | FLJ90231 | 1363 | 1381 | a | A | G | U | U | C | C | U | G | A | U | C | U | U | C | g | G | G | g | 66 | 3 | 14 | c | false | <90% | 3691 | ||
| NM_014802 | KIAA0528 | 1697 | 1715 | a | A | G | U | U | C | C | U | G | A | U | g | U | U | C | U | G | u | U | 61 | 3 | 10 | false | <90% | 4430 | |||
| NM_133378 | TTN | 51439 | 51457 | G | g | G | U | U | C | C | U | G | A | U | C | U | U | C | U | a | G | a | 60 | 3 | 14 | ac | false | <90% | 101519 | ||
| NM_133437 | TTN | 32524 | 32542 | G | g | G | U | U | C | C | U | G | A | U | C | U | U | C | U | a | G | a | 60 | 3 | 14 | ac | false | <90% | 82604 | ||
| NM_133432 | TTN | 32323 | 32341 | G | g | G | U | U | C | C | U | G | A | U | C | U | U | C | U | a | G | a | 60 | 3 | 14 | ac | false | <90% | 82403 | ||
| NM_003319 | TTN | 31948 | 31966 | G | g | G | U | U | C | C | U | G | A | U | C | U | U | C | U | a | G | a | 60 | 3 | 14 | ac | false | <90% | 82028 | ||
| NM_000566 | FCGR1A | 1962 | 1980 | a | c | c | U | U | C | C | U | G | A | U | u | U | U | C | U | G | G | U | 33 | 4 | 8 | false | <90% | 2230 | |||
| NM_018434 | RNF130 | 652 | 670 | G | A | G | U | U | g | C | U | G | A | U | C | a | U | C | U | G | G | g | 21 | 3 | 6 | false | <90% | 1865 | |||
| XM_939083 | TTC28 | 3596 | 3614 | c | A | u | U | U | g | C | U | G | A | U | C | U | U | C | U | G | G | U | 15 | 3 | 13 | bc | false | <90% | 11652 | ||
| XM_929318 | TTC28 | 3596 | 3614 | c | A | u | U | U | g | C | U | G | A | U | C | U | U | C | U | G | G | U | 15 | 3 | 13 | bc | false | <90% | 11653 | ||
| NM_144997 | FLCN | 2781 | 2799 | c | u | c | U | U | C | C | U | c | A | U | C | U | U | C | U | G | G | U | 6 | 4 | 10 | bc | false | <90% | 3717 | ||
| NM_001089 | ABCA3 | 3728 | 3746 | G | A | G | U | U | C | u | U | G | A | U | C | U | U | C | a | G | G | g | 2 | 3 | 8 | c | false | <90% | 6623 | ||
Hits to Sense strand
| Accession | Gene | Start | End | Details of mismatches | Spec. Score | Mismatches | Contiguous | Jackson style specificity | Hits similar gene | HSP Score | HSP % Identity | HSP length | Gene Length | ||||||||||||||||||
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | |||||||||||||
| NM_020116 | FSTL5 | 1185 | 1203 | g | C | C | A | G | A | A | G | A | U | C | A | G | a | A | A | C | U | a | 73 | 3 | 12 | c | false | <90% | 4868 | ||
| XR_018399 | LOC648442 | 463 | 481 | c | C | C | A | G | A | A | G | A | U | C | A | G | u | A | A | C | a | u | 70 | 4 | 12 | c | false | <90% | 880 | ||
| XR_018019 | LOC641777 | 463 | 481 | c | C | C | A | G | A | A | G | A | U | C | A | G | u | A | A | C | a | u | 70 | 4 | 12 | c | false | <90% | 880 | ||
| NM_181786 | HKR1 | 731 | 749 | u | C | C | A | G | A | A | G | A | U | C | A | G | a | A | A | C | a | a | 70 | 4 | 12 | c | false | <90% | 2952 | ||
| NM_153269 | C20orf96 | 90 | 108 | A | C | C | A | G | A | A | G | A | U | C | A | G | G | g | u | C | U | C | 49 | 2 | 14 | c | false | <90% | 1555 | ||
| NM_172195 | EIF2B4 | 1068 | 1086 | A | C | C | A | G | A | A | G | A | U | C | A | G | u | A | A | u | g | g | 49 | 4 | 13 | c | false | <90% | 1719 | ||
| NM_015636 | EIF2B4 | 1109 | 1127 | A | C | C | A | G | A | A | G | A | U | C | A | G | u | A | A | u | g | g | 49 | 4 | 13 | c | false | <90% | 1760 | ||
| NM_001034116 | EIF2B4 | 1112 | 1130 | A | C | C | A | G | A | A | G | A | U | C | A | G | u | A | A | u | g | g | 49 | 4 | 13 | c | false | <90% | 1763 | ||
| NM_152133 | TAGAP | 1481 | 1499 | A | C | C | u | G | A | A | G | A | U | C | A | a | G | A | A | C | U | u | 47 | 3 | 8 | false | <90% | 3196 | |||
| NM_054114 | TAGAP | 1648 | 1666 | A | C | C | u | G | A | A | G | A | U | C | A | a | G | A | A | C | U | u | 47 | 3 | 8 | false | <90% | 3363 | |||
| NM_133378 | TTN | 3945 | 3963 | u | g | u | A | G | A | A | G | A | U | C | A | G | G | A | A | u | U | C | 43 | 4 | 13 | c | false | <90% | 101519 | ||
| NM_133437 | TTN | 3807 | 3825 | u | g | u | A | G | A | A | G | A | U | C | A | G | G | A | A | u | U | C | 43 | 4 | 13 | c | false | <90% | 82604 | ||
| NM_133432 | TTN | 3807 | 3825 | u | g | u | A | G | A | A | G | A | U | C | A | G | G | A | A | u | U | C | 43 | 4 | 13 | c | false | <90% | 82403 | ||
| NM_003319 | TTN | 3807 | 3825 | u | g | u | A | G | A | A | G | A | U | C | A | G | G | A | A | u | U | C | 43 | 4 | 13 | c | false | <90% | 82028 | ||
| NM_133379 | TTN | 3945 | 3963 | u | g | u | A | G | A | A | G | A | U | C | A | G | G | A | A | u | U | C | 43 | 4 | 13 | c | false | <90% | 17306 | ||
| NM_003777 | DNAH11 | 6314 | 6332 | A | C | C | c | G | A | A | G | A | U | C | A | G | G | u | A | C | U | C | 38 | 2 | 10 | c | false | <90% | 14188 | ||
| NM_004003 | CRAT | 793 | 811 | u | g | g | A | G | A | A | G | A | U | C | u | G | G | A | A | C | U | C | 33 | 4 | 8 | false | <90% | 2600 | |||
| NM_000755 | CRAT | 997 | 1015 | u | g | g | A | G | A | A | G | A | U | C | u | G | G | A | A | C | U | C | 33 | 4 | 8 | false | <90% | 2804 | |||
| NM_001024213 | S100A13 | 432 | 450 | A | C | C | u | G | A | A | G | A | U | C | A | G | G | A | A | g | a | a | 32 | 4 | 12 | c | false | <90% | 606 | ||
| NM_001024212 | S100A13 | 464 | 482 | A | C | C | u | G | A | A | G | A | U | C | A | G | G | A | A | g | a | a | 32 | 4 | 12 | c | false | <90% | 638 | ||
| NM_001024211 | S100A13 | 493 | 511 | A | C | C | u | G | A | A | G | A | U | C | A | G | G | A | A | g | a | a | 32 | 4 | 12 | c | false | <90% | 667 | ||
| NM_005979 | S100A13 | 569 | 587 | A | C | C | u | G | A | A | G | A | U | C | A | G | G | A | A | g | a | a | 32 | 4 | 12 | c | false | <90% | 743 | ||
| NM_001024210 | S100A13 | 777 | 795 | A | C | C | u | G | A | A | G | A | U | C | A | G | G | A | A | g | a | a | 32 | 4 | 12 | c | false | <90% | 951 | ||
| NM_001077706 | LFDH | 616 | 634 | A | a | C | A | G | A | A | G | A | U | g | A | G | G | A | A | C | U | a | 22 | 3 | 8 | c | false | <90% | 4261 | ||
| XR_015587 | LOC730833 | 2067 | 2085 | u | C | C | A | G | A | A | a | A | U | C | A | G | G | c | A | C | U | C | 11 | 3 | 6 | c | false | <90% | 11255 | ||
| NM_002016 | FLG | 2067 | 2085 | u | C | C | A | G | A | A | a | A | U | C | A | G | G | c | A | C | U | C | 11 | 3 | 6 | c | false | <90% | 12747 | ||
| NM_016039 | C14orf166 | 262 | 280 | A | C | C | A | G | A | A | a | A | U | C | A | G | G | c | A | C | U | a | 8 | 3 | 7 | c | false | <90% | 1105 | ||
| XM_001129617 | LOC729189 | 995 | 1013 | A | C | C | A | G | A | A | a | A | U | C | A | G | G | A | A | g | g | u | 6 | 4 | 8 | c | false | <90% | 1577 | ||
| XM_001128342 | LOC728744 | 1072 | 1090 | A | C | C | A | G | A | A | a | A | U | C | A | G | G | A | A | g | g | u | 6 | 4 | 8 | c | false | <90% | 1654 | ||
| XM_001133554 | LOC729127 | 1486 | 1504 | A | C | C | A | G | A | A | a | A | U | C | A | G | G | A | A | g | g | u | 6 | 4 | 8 | c | false | <90% | 2068 | ||
| NM_024056 | TMEM106C | 446 | 464 | c | C | C | u | G | A | A | a | A | U | C | A | G | G | A | A | C | U | C | 0 | 3 | 11 | bc | false | <90% | 1449 | ||
| XR_019173 | LOC644203 | 1960 | 1978 | u | C | C | g | G | A | A | c | A | U | C | A | G | G | A | A | C | U | C | 0 | 3 | 11 | bc | false | <90% | 2073 | ||
| NM_005128 | DOPEY2 | 87 | 105 | u | C | C | A | G | A | A | G | A | g | C | A | G | G | A | g | C | U | C | -10 | 3 | 8 | c | false | <90% | 7689 | ||
| NM_017849 | TMEM127 | 2870 | 2888 | A | C | C | A | G | A | A | G | A | c | C | A | G | G | c | A | C | U | g | -12 | 3 | 9 | c | false | <90% | 4223 | ||
| NM_017773 | LAX1 | 2323 | 2341 | u | g | a | A | G | A | A | G | A | a | C | A | G | G | A | A | C | U | C | -15 | 4 | 9 | bc | false | <90% | 3246 | ||
| XR_015587 | LOC730833 | 3042 | 3060 | u | C | C | A | G | A | c | a | A | U | C | A | G | G | A | A | C | U | C | -30 | 3 | 11 | bc | false | <90% | 11255 | ||
| NM_002016 | FLG | 3042 | 3060 | u | C | C | A | G | A | c | a | A | U | C | A | G | G | A | A | C | U | C | -30 | 3 | 11 | bc | false | <90% | 12747 | ||
3 prime UTR Seed matches
Hits to same gene : 0
Hits to other genes : 1398
View matched accessions
3 prime seed match details
Immunostimulatory motif search results
UGUGU - Judge et al. Sequence-dependent stimulation of the mammalian innate immune response by synthetic siRNA. Nat Biotechnol. 2005 Apr;23(4):457-62. (PMID: 15778705)
No Immunostimulatory motif found (UGUGU).
GUCCUUCAA - Hornung et al. Sequence-specific potent induction of IFN-alpha by short interfering RNA in plasmacytoid dendritic cells through TLR7. Nat Med. 2005 Mar;11(3):263-70. Epub 2005 Feb 20. (PMID: 15723075)
No Immunostimulatory motif found (GUCCUUCAA).
Settings used for search
Scores were calculated using scoring scheme SS1
Using AVG scoring mode.
Hit scores
| Parameter | Spec. Score cut-off | Colour | Comment |
| Hit to same gene | Colour | Hits to the same accession or genes with 100 percent identity (i.e. likely transcript variants). It is advisable to always check the genes hit. | |
| High scoring hit | ≥80 | Colour | Hits are very likely to cause an off-target effect |
| Medium scoring hit | <80¸≥40 | Colour | Hits with a small chance of causing an off target effect. |
| Low scoring hit | Colour | Hits very unlikely to cause an off-target effect |
The mismatches are colour coded based on the weighting of that position.
| Cut-off | Colour |
| Match | Colour |
| Wobble base pairs (G-U) | Colour |
| Critical bases (none) | Colour |
| 0.6 bits | Colour |
| 0.4 bits | Colour |
| 0.2 bits | Colour |
SpecificityServer is designed to help you identify potential non-specific matches to your siRNA. It incorporates the latest information about non-specific matches (sequence-specific only).
Designed by: Alistair Chalk, Quan Du, Zicai Liang and Erik L.L. Sonnhammer