Automatic recommendation
OK - siRNA is specific for both sense and antisense (guide) strands
8 hits were found for AGCGCACAAGGAAGGCGUG (5 guide strand hits and 3 sense strand hits.)
Hits to Guide strand
| Accession | Gene | Start | End | Details of mismatches | Spec. Score | Mismatches | Contiguous | Jackson style specificity | Hits similar gene | HSP Score | HSP % Identity | HSP length | Gene Length | ||||||||||||||||||
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | |||||||||||||
| NM_032373 | PCGF5 | 1811 | 1829 | A | u | u | G | C | A | C | A | A | G | G | A | A | G | G | C | a | U | G | 45 | 3 | 13 | c | false | <90% | 7179 | ||
| NM_032431 | SYVN1 | 2700 | 2718 | A | G | C | G | C | c | C | A | A | G | G | A | A | G | G | C | c | a | c | 5 | 4 | 10 | c | false | <90% | 3071 | ||
| NM_172230 | SYVN1 | 2703 | 2721 | A | G | C | G | C | c | C | A | A | G | G | A | A | G | G | C | c | a | c | 5 | 4 | 10 | c | false | <90% | 3074 | ||
| XM_927032 | LOC653348 | 789 | 807 | g | G | C | G | C | g | C | A | A | G | c | A | A | G | G | C | G | U | G | -26 | 3 | 8 | c | false | <90% | 1983 | ||
| NM_014694 | ADAMTSL2 | 1877 | 1895 | g | G | C | G | C | g | C | A | A | G | c | A | A | G | G | C | G | U | G | -26 | 3 | 8 | c | false | <90% | 3740 | ||
Hits to Sense strand
| Accession | Gene | Start | End | Details of mismatches | Spec. Score | Mismatches | Contiguous | Jackson style specificity | Hits similar gene | HSP Score | HSP % Identity | HSP length | Gene Length | ||||||||||||||||||
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | |||||||||||||
| NM_002383 | MAZ | 2483 | 2501 | C | A | C | c | C | C | U | U | C | C | U | U | u | U | G | C | G | C | g | 47 | 3 | 8 | false | <90% | 2564 | |||
| NM_001042539 | MAZ | 2708 | 2726 | C | A | C | c | C | C | U | U | C | C | U | U | u | U | G | C | G | C | g | 47 | 3 | 8 | false | <90% | 2789 | |||
| NM_020882 | COL20A1 | 2803 | 2821 | a | u | C | G | u | C | U | U | C | C | U | U | G | U | G | C | G | C | c | 30 | 4 | 13 | bc | false | <90% | 4187 | ||
3 prime UTR Seed matches
Hits to same gene : 0
Hits to other genes : 279
View matched accessions
3 prime seed match details
Immunostimulatory motif search results
UGUGU - Judge et al. Sequence-dependent stimulation of the mammalian innate immune response by synthetic siRNA. Nat Biotechnol. 2005 Apr;23(4):457-62. (PMID: 15778705)
No Immunostimulatory motif found (UGUGU).
GUCCUUCAA - Hornung et al. Sequence-specific potent induction of IFN-alpha by short interfering RNA in plasmacytoid dendritic cells through TLR7. Nat Med. 2005 Mar;11(3):263-70. Epub 2005 Feb 20. (PMID: 15723075)
No Immunostimulatory motif found (GUCCUUCAA).
Settings used for search
Scores were calculated using scoring scheme SS1
Using AVG scoring mode.
Hit scores
| Parameter | Spec. Score cut-off | Colour | Comment |
| Hit to same gene | Colour | Hits to the same accession or genes with 100 percent identity (i.e. likely transcript variants). It is advisable to always check the genes hit. | |
| High scoring hit | ≥80 | Colour | Hits are very likely to cause an off-target effect |
| Medium scoring hit | <80¸≥40 | Colour | Hits with a small chance of causing an off target effect. |
| Low scoring hit | Colour | Hits very unlikely to cause an off-target effect |
The mismatches are colour coded based on the weighting of that position.
| Cut-off | Colour |
| Match | Colour |
| Wobble base pairs (G-U) | Colour |
| Critical bases (none) | Colour |
| 0.6 bits | Colour |
| 0.4 bits | Colour |
| 0.2 bits | Colour |
SpecificityServer is designed to help you identify potential non-specific matches to your siRNA. It incorporates the latest information about non-specific matches (sequence-specific only).
Designed by: Alistair Chalk, Quan Du, Zicai Liang and Erik L.L. Sonnhammer