Automatic recommendation
OK - siRNA is specific for both sense and antisense (guide) strands
12 hits were found for ACCUCUAUGUAUCCCUCCU (8 guide strand hits and 4 sense strand hits.)
Hits to Guide strand
| Accession | Gene | Start | End | Details of mismatches | Spec. Score | Mismatches | Contiguous | Jackson style specificity | Hits similar gene | HSP Score | HSP % Identity | HSP length | Gene Length | ||||||||||||||||||
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | |||||||||||||
| NM_016272 | TOB2 | 4178 | 4196 | u | C | C | U | C | U | A | U | c | U | A | U | C | C | C | U | C | u | U | 35 | 3 | 8 | c | false | <90% | 4575 | ||
| NM_020774 | MIB1 | 5673 | 5691 | u | C | C | U | C | U | c | U | G | U | A | U | C | C | C | U | C | a | c | 24 | 4 | 10 | c | false | <90% | 9308 | ||
| NM_004411 | DYNC1I1 | 466 | 484 | A | C | C | c | C | U | A | U | G | U | c | U | C | C | C | U | C | C | U | 1 | 2 | 8 | c | false | <90% | 2942 | ||
| XR_018837 | LOC648190 | 929 | 947 | u | C | C | c | C | U | A | U | G | U | g | U | C | C | C | U | C | C | U | 0 | 3 | 8 | c | false | <90% | 2830 | ||
| XR_017572 | LOC645978 | 929 | 947 | u | C | C | c | C | U | A | U | G | U | g | U | C | C | C | U | C | C | U | 0 | 3 | 8 | c | false | <90% | 2830 | ||
| NM_001010887 | ASAH3L | 931 | 949 | u | C | C | c | C | U | A | U | G | U | g | U | C | C | C | U | C | C | U | 0 | 3 | 8 | c | false | <90% | 2852 | ||
| NM_152440 | FLJ32549 | 1733 | 1751 | g | C | C | U | C | U | c | U | G | U | u | U | C | C | C | U | C | C | U | -29 | 3 | 8 | c | false | <90% | 2583 | ||
| NM_144633 | KCNH8 | 2932 | 2950 | A | C | C | U | C | U | g | U | G | U | g | U | C | C | C | U | C | C | a | -32 | 3 | 7 | c | false | <90% | 5106 | ||
Hits to Sense strand
| Accession | Gene | Start | End | Details of mismatches | Spec. Score | Mismatches | Contiguous | Jackson style specificity | Hits similar gene | HSP Score | HSP % Identity | HSP length | Gene Length | ||||||||||||||||||
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | |||||||||||||
| NM_006062 | SMYD5 | 1987 | 2005 | A | G | G | A | G | G | G | A | c | A | C | A | U | A | G | u | G | G | g | 8 | 3 | 8 | c | false | <90% | 2561 | ||
| XM_928706 | KIAA0754 | 15 | 33 | A | G | G | A | u | G | G | c | U | A | C | A | U | A | G | A | G | G | a | -18 | 3 | 10 | bc | false | <90% | 6171 | ||
| XM_941912 | KIAA0754 | 71 | 89 | A | G | G | A | u | G | G | c | U | A | C | A | U | A | G | A | G | G | a | -18 | 3 | 10 | bc | false | <90% | 6227 | ||
| NM_001080421 | UNC13A | 9119 | 9137 | u | G | G | A | G | a | G | A | g | A | C | A | U | A | G | A | G | G | U | -25 | 3 | 10 | bc | false | <90% | 10102 | ||
3 prime UTR Seed matches
Hits to same gene : 0
Hits to other genes : 1332
View matched accessions
3 prime seed match details
Immunostimulatory motif search results
UGUGU - Judge et al. Sequence-dependent stimulation of the mammalian innate immune response by synthetic siRNA. Nat Biotechnol. 2005 Apr;23(4):457-62. (PMID: 15778705)
No Immunostimulatory motif found (UGUGU).
GUCCUUCAA - Hornung et al. Sequence-specific potent induction of IFN-alpha by short interfering RNA in plasmacytoid dendritic cells through TLR7. Nat Med. 2005 Mar;11(3):263-70. Epub 2005 Feb 20. (PMID: 15723075)
No Immunostimulatory motif found (GUCCUUCAA).
Settings used for search
Scores were calculated using scoring scheme SS1
Using AVG scoring mode.
Hit scores
| Parameter | Spec. Score cut-off | Colour | Comment |
| Hit to same gene | Colour | Hits to the same accession or genes with 100 percent identity (i.e. likely transcript variants). It is advisable to always check the genes hit. | |
| High scoring hit | ≥80 | Colour | Hits are very likely to cause an off-target effect |
| Medium scoring hit | <80¸≥40 | Colour | Hits with a small chance of causing an off target effect. |
| Low scoring hit | Colour | Hits very unlikely to cause an off-target effect |
The mismatches are colour coded based on the weighting of that position.
| Cut-off | Colour |
| Match | Colour |
| Wobble base pairs (G-U) | Colour |
| Critical bases (none) | Colour |
| 0.6 bits | Colour |
| 0.4 bits | Colour |
| 0.2 bits | Colour |
SpecificityServer is designed to help you identify potential non-specific matches to your siRNA. It incorporates the latest information about non-specific matches (sequence-specific only).
Designed by: Alistair Chalk, Quan Du, Zicai Liang and Erik L.L. Sonnhammer