Predicted Off-targets for siRNA "cucaguuuacuagugccauuuguuc" Using siRNA SpecificityServer

Specificity Server [Result page v1.0]


SpecificityServer - An siRNA design program for determining siRNA specificity

Automatic recommendation
OK - siRNA is specific for both sense and antisense (guide) strands

9 hits were found for UUACUAGUGCCAUUUGUUC (5 guide strand hits and 4 sense strand hits.)

Hits to Guide strand

AccessionGeneStartEndDetails of mismatchesSpec.
Score
MismatchesContiguousJackson style
specificity
Hits similar
gene
HSP
Score
HSP %
Identity
HSP lengthGene Length
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19
XM_940803 LOC134121 252 270 cagCUAGUGCCAUUUGUUg62 4 15 bc false   <90%   2773
XM_376372 LOC134121 253 271 cagCUAGUGCCAUUUGUUg62 4 15 bc false   <90%   2774
NR_003024 LOC442720 469 487 UcACUAGUGCCAUUUGggg57 4 14 ac false   <90%   987
NM_032842 FLJ14803 1162 1180 aUAuUAGUGCCAcUUGUUC50 3 8   false   <90%   3560
NM_138347 ZNF551 3137 3155 agACUgGUGCCAUUUGUUg18 4 12 bc false   <90%   3669


Hits to Sense strand





AccessionGeneStartEndDetails of mismatchesSpec.
Score
MismatchesContiguousJackson style
specificity
Hits similar
gene
HSP
Score
HSP %
Identity
HSP lengthGene Length
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19
XM_940803 LOC134121 2590 2608 aAACAAAUGGCAgUAGUuu79 4 11   false   <90%   2773
XM_376372 LOC134121 2591 2609 aAACAAAUGGCAgUAGUuu79 4 11   false   <90%   2774
NM_014611 MDN1 13480 13498 ucACAAAUGGCACUAGUug78 4 15 ac false   <90%   17400
XR_016124 LOC646214 410 428 ucACAAAUGGCuCUAGUAc47 4 9   false   <90%   6629


3 prime UTR Seed matches

Hits to same gene : 0
Hits to other genes : 1230
View matched accessions

3 prime seed match details

AccessionGene nameHits to this gene
NM_004719SFRS2IP4
NM_021238FAM60A3
NM_138270ATRX3
NM_000489ATRX3
XM_001128623LOC7288493
NM_207406CCDC43
NM_032012C9orf53
NM_0010997343
NM_001024843TNRC6B3
NM_015088TNRC6B3
NM_006626ZBTB63
XM_931553LOC6434493
XM_945026LOC6434493
NM_007175ERLIN22
NM_004736XPR12
NM_006654FRS22
NM_001042555FRS22
NM_153267MAMDC22
NM_014480ZNF5442
NM_173484KLF172
NM_021629GNB42
NM_001012659ARGFX2
NM_000486AQP22
NM_001025073C3orf172
NM_015412C3orf172
NM_001025072C3orf172
NM_001040429PCDH172
NM_152756RICTOR2
NM_005665EVI52
NM_002126HLF2
NM_006451PAIP12
NM_182789PAIP12
NM_183323PAIP12
NM_198581ZC3H62
NM_014309RBM92
NM_001082576RBM92
NM_001082577RBM92
NM_001031695RBM92
NM_001082578RBM92
NM_001082579RBM92
NM_001018065NTRK22
NM_001018066NTRK22
NM_152874FAS2
NM_152876FAS2
NM_152877FAS2
NM_152872FAS2
NM_152873FAS2
NM_152875FAS2
NM_175861TMTC12
NM_0010937252
NM_152281SCYL1BP12
NM_138959VANGL12
NM_001025108AFF32
NM_002285AFF32
NM_020148SPIRE12
NM_013231FLRT22
NM_032145FBXO302
NM_0010087812
XM_936538FAT32
XM_926199FAT32
NM_001957EDNRA2
NM_025059C6orf972
NM_024761MOBKL2B2
NM_006933SLC5A32
NM_006055LANCL12
NM_007249KLF122
NM_152265BTF3L42
NM_017752TBC1D8B2
NM_015247CYLD2
NM_001042412CYLD2
NM_001042355CYLD2
NM_020453ATP10D2
NM_020342SLC39A102
NM_001025390AMPD32
NM_001025389AMPD32
NM_000480AMPD32
NM_001079526IKZF22
NM_016260IKZF22
NM_000609CXCL122
NM_001351DAZL2
NM_001029884PLEKHG12
NM_024913FLJ219862
NM_138715MSR12
NM_138716MSR12
NM_003034ST8SIA12
NM_019114EPB41L4B2
NM_001010846SHE2
NM_153825SLA/LP2
NM_021190PTBP21
XM_940362LOC6459841
XM_928946LOC6459841
NM_021269ZNF7081
NM_031418TMEM16C1
NM_177948ARMCX31
NM_177947ARMCX31
NM_016607ARMCX31
NM_020340KIAA12441
NM_015265SATB21
NM_025027ZNF6061
NM_053042KIAA17291
NM_054027ANKH1
XM_001132013ANKH1
NM_007202AKAP101
NM_006282STK41
NM_001848COL6A11
XM_001131911LOC6459041
XM_938424LOC6459041
XM_928876LOC6459041
NM_001032280TFAP2A1
NM_003220TFAP2A1
NM_001042425TFAP2A1
NM_001008537KIAA20221
NM_052860ZNF3001
NM_0010854471
NM_021127PMAIP11
NM_133448TMEM132D1
NM_004440EPHA71
NM_018313PBRM11
NM_181042PBRM11
NM_018165PBRM11
NM_015002FBXO211
NM_033624FBXO211
NM_0208461
NM_000651CR11
NM_000573CR11
NM_017418DEC11
NM_207361FREM21
NM_020805KLHL141
NM_006988ADAMTS11
NM_015026MON21
NM_173808NEGR11
NM_022445TPK11
NM_001042482TPK11
NM_182853CREM1
NM_182722CREM1
NM_182717CREM1
NM_182721CREM1
NM_183012CREM1
NM_183011CREM1
NM_182723CREM1
NM_182720CREM1
NM_183013CREM1
NM_053282SH2D1B1
NM_173851SLC30A81
NM_001011553SEPT71
NM_001788SEPT71
NM_1522831
NM_004282BAG21
NM_004529MLLT31
NM_007085FSTL11
NM_000337SGCD1
NM_024610HSPBAP11
XM_001128917LOC7312401
XM_001126538LOC7281031
NM_181354OXR11
NM_004339PTTG1IP1
NM_007110TEP11
NM_018023YEATS21
NM_001018067SERBP11
NM_015640SERBP11
NM_001018069SERBP11
NM_001018068SERBP11
NM_003899ARHGEF71
XM_942773LOC6423451
XM_930726LOC6423451
NM_001013620ALG10B1
NM_017637BNC21
NM_002357MXD11
NM_020802KIAA13771
NM_152491FLJ325691
NM_201648GLYAT1
NM_004411DYNC1I11
NM_024590ARSJ1
NM_015275KIAA10331
NM_022475HHIP1
NM_015954DERA1
NM_000880IL71
NM_022091ASCC31
NM_005897IPP1
NM_005596NFIB1
NM_003927MBD21
NM_023037FRY1
NM_000991RPL281
NM_001008391CCDC731
NM_015979CRSP31
XM_049078KIAA12391
XM_940885KIAA12391
NM_178505TMEM261
NM_133474ZNF7211
NM_002737PRKCA1
NM_004727SLC24A11
NM_019119PCDHB91
NM_024624SMC61
NM_014838ZBED41
NM_001014797KCNMA11
NM_006375ENOX21
NM_182314ENOX21
NM_002202ISL11
NM_030579CYB5B1
NM_020403PCDH91
NM_203487PCDH91
NM_175056ZPLD11
NM_015092SMG11
NM_004337OSGIN21
NM_002940ABCE11
NM_001040876ABCE11
NM_006991ZNF1971
NM_006606RBBP91
NM_015571SENP61
NM_0011004091
NM_000866HTR1F1
NM_014369PTPN181
NM_152785GCET21
NM_001008756GCET21
NM_001007527LMBRD21
NM_004293GDA1
NM_006731FCMD1
NM_001079802FCMD1
NM_138782FCHO21
NM_013235RNASEN1
NM_0011004121
NM_002182IL1RAP1
NM_030791SGPP11
NM_001023567GOLGA8B1
NM_181077GOLGA8A1
NM_001012420GOLGA8G1
NM_001012423GOLGA8E1
NM_173514FLJ907091
NM_001077691ALG91
NM_001077692ALG91
NM_024740ALG91
NM_001077690ALG91
NM_018010IFT571
NM_006158NEFL1
NM_004346CASP31
NM_032991CASP31
NM_080737SYTL41
NM_004028AQP41
NM_001650AQP41
NM_022831C1orf801
NM_032228MLSTD21
NM_078485COL9A11
NM_001851COL9A11
NM_001077440BCLAF11
NM_001077441BCLAF11
NM_014739BCLAF11
NM_016651DACT11
NM_001079520DACT11
NM_002828PTPN21
NM_004085TIMM8A1
NM_020803KLHL81
NM_014400LYPD31
NM_012388PLDN1
NM_020935USP371
NM_001044387ZNF5571
NM_024341ZNF5571
NM_001044388ZNF5571
NM_002852PTX31
NM_003873NRP11
NM_003852TRIM241
NM_015905TRIM241
NM_016231NLK1
NM_001280CIRBP1
NM_022726ELOVL41
NM_001031679MSRB31
NM_198080MSRB31
NM_001006623WDR331
NM_001079846CREBBP1
NM_004380CREBBP1
NM_001103ACTN21
NM_001039547GK51
NM_014728FRMPD41
NM_005436CCDC61
NM_003489NRIP11
NM_002736PRKAR2B1
NM_004522KIF5C1
NM_000809GABRA41
NM_025048GPR1101
NM_139277KLK71
NM_005046KLK71
NM_006883SHOX1
XM_937783Rg9mtd11
XM_067605Rg9mtd11
NM_139057ADAMTS171
NM_001081461JMJD61
NM_182609ZNF6771
NM_018449UBAP21
NM_032154PCGF61
NM_001011663PCGF61
NM_0010996421
NM_018348FLJ111711
NM_001080421UNC13A1
NM_001024847TGFBR21
NM_003242TGFBR21
NM_005730CTDSP21
NM_012290TLK11
NM_017658KLHL281
NM_016026RDH111
NM_005941MMP161
NM_015317PUM21
NM_001082480ZNF6231
NM_014789ZNF6231
NM_031483ITCH1
NM_0011056631
NM_020772NUFIP21
NM_182688UBE2G21
NM_003343UBE2G21
XM_378661C17orf511
XM_944416C17orf511
NM_194282LIN541
NM_001039349EFEMP11
NM_004105EFEMP11
NM_001039348EFEMP11
NM_006671SLC1A71
NM_020467LOC572281
NM_001031628LOC572281
NM_001033873LOC572281
NM_152871FAS1
NM_000043FAS1
NM_015313ARHGEF121
NM_017861PIGX1
NM_031844HNRPU1
NM_004501HNRPU1
NM_0152411
XM_001128811LOC7312101
NM_016448DTL1
XM_370909LOC3881771
XM_938956LOC3881771
NM_031458PARP91
XM_001128974DDB11
NM_020341PAK71
NM_177990PAK71
NM_003045SLC7A11
NM_138777MRRF1
NM_199177MRRF1
NM_020133AGPAT41
NM_033240PML1
NM_001008493ENAH1
NM_018212ENAH1
NM_001035513SDHC1
NM_001035512SDHC1
NM_003001SDHC1
NM_001035511SDHC1
NM_199242UNC13D1
NM_172070ZNF6501
NM_020774MIB11
NM_024709C1orf1151
NM_152253CHKB1
NM_173478CNTD11
NM_000944PPP3CA1
NM_178509STXBP41
NM_003800RNGTT1
NM_001931DLAT1
NM_002547OPHN11
NM_014668GREB11
NM_005830MRPS311
NM_003679KMO1
NM_004628XPC1
NM_024792FAM57A1
NM_033544RCCD11
NM_001017919RCCD11
NM_152701ABCA131
NM_001025266LOC2853821
NM_052904KLHL321
NM_002718PPP2R3A1
NM_181897PPP2R3A1
NM_001077203SENP71
NM_020654SENP71
NM_207413UNQ94331
NM_032521PARD6B1
NM_198460GBP61
NM_003768PEA151
NM_025191EDEM31
NM_000351STS1
NM_153262SYT141
NM_006154NEDD41
NM_198400NEDD41
NM_018718TSGA141
NM_022048CSNK1G11
NM_016626RKHD21
NM_018303EXOC21
NM_002870RAB131
XM_928167LOC6451371
XM_001127034LOC7280801
XM_001127051LOC7280801
XM_001127002LOC7280801
XM_001127022LOC7280801
XM_001126877LOC7280471
XM_001126928LOC7280471
XM_001126392LOC7279091
XM_001126912LOC7280471
XM_001126407LOC7279091
XM_926998LOC6436991
XM_001126378LOC7279091
XM_001126904LOC7280471
NM_0011016761
NM_152344LSM121
NM_006510TRIM271
NM_001001732C10orf1301
NM_024052C17orf391
NM_145044ZNF5011
NM_000369TSHR1
NM_000271NPC11
NM_144582TEX2611
NM_032558HIATL11
NM_173641EPHA101
NM_024501HOXD11
NM_032466ASPH1
NM_032468ASPH1
NM_017444CHRAC11
NM_003644GAS71
NM_201432GAS71
NM_201433GAS71
NM_001012393OPCML1
NM_002545OPCML1
NM_004888ATP6V1G11
NM_014232VAMP21
NM_032824TMEM87B1
NM_000538RFXAP1
NM_181356SUPT3H1
NM_001432EREG1
XM_001129280LOC7290861
NM_030796ECOP1
XM_938287LOC6539301
NM_001017930WDR42B1
NM_198506FLJ446911
NM_014586HUNK1
NM_152570LINGO21
NM_014965TRAK11
NM_015200SCC-1121
NM_0011003991
NM_152851MS4A6A1
NM_152852MS4A6A1
NM_017813IMPAD11
NM_014585SLC40A11
NM_152347C17orf571
NM_016485VTA11
NM_001001709FLJ455371
NM_198904GABRG21
NM_000816GABRG21
NM_198903GABRG21
NM_013995LAMP21
NM_145733SEPT31
NM_019106SEPT31
NM_001439EXTL21
NM_001033025EXTL21
NM_002716PPP2R1B1
XM_001133620LOC7318951
NM_001080396LOC7282151
NM_003693SCARF11
NM_145351SCARF11
NM_145350SCARF11
NM_145349SCARF11
NM_145352SCARF11
NM_014873LPGAT11
NM_153181NETO11
NM_138966NETO11
NM_018230NUP1331
NM_145274TMEM991
NM_014415ZBTB111
NM_032121DKFZp564K1421
XM_001128660LOC7288661
NM_022783DEPDC61
NM_003114SPAG11
NM_172218SPAG11
NM_182641BPTF1
NM_004459BPTF1
NM_018388MBNL31
NM_018466ALG131
NM_0010987901
NM_021242MID1IP11
NM_0010987911
NM_006614CHL11
NM_1779662'-PDE1
NM_024948C10orf971
NM_145257C1orf961
NM_032102SFRS2B1
NM_080927DCBLD21
NM_015902EDD11
NM_130807MOBKL2A1
NM_006425SLU71
NM_033067DMRTB11
NM_013347RPA41
NM_021191NEUROD41
NM_005938MLLT71
NM_005392PHF21
NM_005154USP81
NM_172071RC3H11
NM_183050BCKDHB1
NM_175854PAN31
NM_020207C9orf1021
NM_016829OGG11
NM_016827OGG11
NM_016828OGG11
NM_145259ACVR1C1
NM_0011110311
NM_0011110331
NM_0011110321
NM_020116FSTL51
NM_015570AUTS21
NM_019592RNF201
NM_001080437SNED11
XM_940984SNED11
NM_182832PLAC41
NM_144628TBC1D201
NM_152529GPR1551
NM_001033045GPR1551
NM_194285SPTY2D11
NM_001009899KIAA20181
NM_001033056GLUL1
NM_002065GLUL1
NM_001033044GLUL1
NM_000046ARSB1
NM_021823PPCDC1
NM_152603ZNF5671
NM_006564CXCR61
NM_203365RAPH11
NM_005935AFF11
NM_152722HEPACAM1
NM_177424STX121
NM_194434VAPA1
NM_003574VAPA1
NM_145034TOR1AIP21
NM_015483KBTBD21
NM_207372SH2D4B1
NM_014743KIAA02321
NM_0011005901
NM_194317LYPD61
NM_013989DIO21
NM_001007023DIO21
NM_000793DIO21
NM_021148ZNF2731
NM_001012756ZNF2601
NM_0195891
NM_181714C6orf1521
NM_001071775LOC4401451
NM_033087ALG21
NM_0212141
NM_018121C10orf61
NM_015060KIAA02411
NM_001017523BTBD111
NM_001018072BTBD111
NM_006373VAT11
NM_003462DNALI11
NM_022473ZFP1061
NM_001921DCTD1
NM_001012732DCTD1
NM_175768GRIK21
NM_021956GRIK21
NM_024958NRSN21
NM_003202TCF71
NM_201632TCF71
NM_213648TCF71
NM_201634TCF71
XM_001130507LOC7317081
XM_001127823LOC7285731
NM_181844BCL6B1
NM_019063EML41
NM_001338CXADR1
NM_213674TPM21
NM_000390CHM1
NM_020673RAB22A1
NM_001859SLC31A11
XM_939804LOC6453201
XM_928359LOC6453201
NM_001080412ZBTB381
XM_001133510ZBTB381
NM_006252PRKAA21
NM_019050USP531
NM_004747DLG51
NM_001040446MTMR121
NM_005783TXNDC91
NM_005748YAF21
XM_945588FLJ303751
XM_498945FLJ303751
NM_007078LDB31
NM_001080114LDB31
NM_018361AGPAT51
NM_005807PRG41
NM_020718USP311
NM_024665TBL1XR11
NM_014982PCNX1
NM_032199ARID5B1
NM_000875IGF1R1
NM_182758WDR721
NM_001004317LIN28B1
NM_018362LIN7C1
XM_001133760ZNF5191
NM_001024647RAB3IP1
NM_022456RAB3IP1
NM_175624RAB3IP1
NM_175625RAB3IP1
NM_175623RAB3IP1
NM_003221TFAP2B1
NM_174914UGT3A21
NM_052905FMNL21
NM_016357LIMA11
NM_0011113191
NM_015507EGFL61
NM_145032FBXL131
NM_0011110381
NM_015206KIAA10241
NM_016019LUC7L21
NM_005239ETS21
NM_173552C3orf581
NM_000926PGR1
NM_001010922C1orf1781
NM_001043229LOC7510711
NM_144968RIBC11
NM_023112OTUB21
NM_021132PPP3CB1
NM_005373MPL1
NM_145006SUSD31
NM_032731TXNL51
NM_006299ZNF1931
NM_024577SH3TC21
NM_032730RTN4IP11
NM_017935BANK11
NM_001083907BANK11
NM_015912C8ORFK321
NM_018191RCBTB11
NM_032825ZNF3821
NM_198476C19orf541
NM_002655PLAG11
NM_031924RSHL21
NM_018283NUDT151
NM_148957TNFRSF191
NM_018584CAMK2N11
NM_025000C2orf371
NM_001025249DUT1
NM_001025248DUT1
NM_001948DUT1
NM_030934C1orf251
NM_145166ZBTB471
NM_002594PCSK21
NM_152581MOSPD21
NM_182692SRPK21
NM_182691SRPK21
NM_0147221
NM_0011001641
NM_0011001661
NM_014721PHACTR21
NM_0011001651
NM_004866SCAMP11
NM_012309SHANK21
NM_003112SP41
NM_018400SCN3B1
NM_001040151SCN3B1
NM_016644PRR161
NM_022770GINS31
NM_138459NUS11
NM_003921BCL101
NM_004744LRAT1
NM_152734C6orf891
NM_001690ATP6V1A1
NM_020766PCDH191
NM_0011052431
NM_001001664LOC3397451
NM_022080NAPB1
NM_001040057MGC404051
NM_152789MGC404051
XM_933646LOC6464841
XM_943429LOC6464841
NM_033551LARP11
NM_015315LARP11
NM_001045478LOC6449971
NM_018233OGFOD11
NM_013943CLIC41
NM_001002926TWISTNB1
NM_018373SYNJ2BP1
NM_025214CCDC681
NM_033364C3orf151
NM_017849TMEM1271
NM_022047DEF61
NM_012416RANBP61
NM_005069SIM21
NM_012461TINF21
NM_012141INTS61
NM_001039937INTS61
NM_031453FAM107B1
NM_0204591
NM_145687MAP4K41
NM_145686MAP4K41
NM_004834MAP4K41
NM_020917ZFP141
NM_006581FUT91
NM_030954RNF1701
NM_014207CD51
NM_001079864TAX1BP11
NM_006024TAX1BP11
NM_145246C10orf41
NM_182975ZNF3261
NM_016052RRP151
NM_016156MTMR21
NM_201278MTMR21
NM_201281MTMR21
NM_152361EID2B1
NM_173509C1orf761
NM_173648FLJ395021
NM_017641KIF21A1
NM_145207SPATA51
NM_016436PHF201
NM_174890ANUBL11
NM_020783SYT41
NM_003262TLOC11
NM_016426GTSE11
NM_000808GABRA31
NM_0247761
XM_001129439LOC7308771
XM_001127905LOC7281961
NM_018364RSBN11
NM_0011026031
NM_198893ZNF1601
NM_033288ZNF1601
NM_182515ZNF7141
NM_014033METTL7A1
NM_021645UTP14C1
NM_020778ALPK31
NM_153711FAM26E1
NM_013332HIG21
NM_0010987861
NM_021635PBOV11
NM_013411AK21
NM_022897RANBP171
NM_006941SOX101
NM_014112TRPS11
NM_0207211
NM_001009186CCT6A1
NM_001762CCT6A1
NM_025190KIAA16411
NM_001040280CD831
NM_004233CD831
NM_016507CRKRS1
NM_0150831
NM_017801CMTM61
NM_178844NLRC31
NM_015185ARHGEF91
NM_024700SNIP11
NM_030583MATN21
NM_002380MATN21
NM_022725FANCF1
NM_014141CNTNAP21
NM_197949CLEC7A1
NM_197950CLEC7A1
NM_022570CLEC7A1
NM_197948CLEC7A1
NM_197947CLEC7A1
NM_014911AAK11
NM_032973PCDH11Y1
NM_032968PCDH11X1
NM_032969PCDH11X1
NM_0011128001
NM_021097SLC8A11
NM_0011128011
NM_0011128021
NM_0010853681
NM_001009960ZNF6551
NM_0010839561
NM_138494ZNF6551
NM_005082TRIM251
NM_030937CCNL21
NM_001039577CCNL21
XM_001126014LOC7278771
NM_001077401ACVRL11
NM_000020ACVRL11
NM_005127CLEC2B1
NM_032295SLC37A31
NM_207113SLC37A31
NM_020640DCUN1D11
NM_000912OPRK11
NM_198846SIGLEC61
NM_198845SIGLEC61
NM_001245SIGLEC61
NM_032486DCTN51
NM_001626AKT21
NM_194356STX21
NM_001980STX21
NM_153456HS6ST31
NM_014805EPM2AIP11
NM_019045WDR441
NM_005476GNE1
NM_018685ANLN1
NM_001002860BTBD71
NM_015039NMNAT21
NM_170706NMNAT21
NM_175921LOC2856361
NM_194071CREB3L21
NM_018374TMEM106B1
NM_006408AGR21
NM_021083XK1
XM_001134341FLJ206741
NM_018353C14orf1061
NM_001079685KIAA11911
NM_020444KIAA11911
NM_001079684KIAA11911
NM_005611RBL21
NM_001004434SLC30A21
NM_032513SLC30A21
NM_018696ELAC11
NM_020245TULP41
NM_001007466TULP41
NM_030648SETD71
NM_138381OXNAD11
NM_024511C4orf151
NM_000555DCX1
NM_178152DCX1
NM_178153DCX1
NM_178151DCX1
NM_001037442RUFY31
NM_138392SHKBP11
NM_199461NANOS11
NM_005222DLX61
NM_002349LY751
NM_018200HMG20A1
NM_003588CUL4B1
NM_001079872CUL4B1
NM_001039780FLJ167931
NM_020921NIN1
NM_001029858SLC35F11
NM_005523HOXA111
XM_001130346LOC7294681
NM_213589RAPH11
NM_001080379PACRG1
NM_001080378PACRG1
NM_152410PACRG1
NM_005873RGS191
NM_001039467RGS191
NM_015347RIMBP21
NM_024772ZMYM11
NM_145048C4orf281
NM_006364SEC23A1
NM_001080415SR1401
XM_940802SR1401
NM_006275SFRS61
NM_181783TMTC31
NM_145735ARHGEF71
NM_004170SLC1A11
NM_012073CCT51
NM_001013655LOC3898341
XM_001125792LOC7278341
XM_926013LOC6423981
NM_207435FLJ401421
NM_139164STARD41
NM_016024RBMX21
NM_020951ZNF5291
NM_172239REXO1L11
NM_152624DCP21
NM_052811TRIM131
NM_213590TRIM131
NM_001007278TRIM131
NM_005798TRIM131
NM_020781ZNF3981
NM_170686ZNF3981
NM_018660ZNF3951
XM_037557KIAA09841
XM_939476KIAA09841
XM_001124844LOC7304201
NM_032967PCDH11X1
NM_032972PCDH11Y1
NM_032971PCDH11Y1
XM_001127359LOC7305571
XM_001125998LOC7278271
XM_001132378PTP4A21
XM_001132367PTP4A21
XM_001132372PTP4A21
XM_001132369PTP4A21
XM_001132357PTP4A21
NM_0146431
NM_024989PGAP11
NM_007137ZNF811
NM_138731MIPOL11
NM_020782KLHDC51
NM_005443PAPSS11
NM_002264KPNA11
NM_015464SOSTDC11
NM_198566C5orf341
NM_001037317PAP2D1
NM_001010861PAP2D1
NM_194329RNF381
NM_194328RNF381
NM_194330RNF381
NM_022781RNF381
NM_194332RNF381
NM_031448C19orf121
NM_001031726C19orf121
NM_003617RGS51
NM_0155621
NM_001042697ZSWIM71
NM_001042698ZSWIM71
NM_003203C2orf31
NM_000084CLCN51
NM_001010971SAMD131
NM_003153STAT61
NM_033132ZIC51
NM_181847AMIGO21
NM_032221CHD61
NM_014846KIAA01961
NM_007123USH2A1
NM_002376MARK31
NM_003471KCNAB11
NM_172159KCNAB11
NM_172160KCNAB11
NM_203451LOC4001201
NM_004985KRAS1
NM_033360KRAS1
NM_016338IPO111
NM_183397PXMP41
NM_007238PXMP41
NM_153645NUP501
NM_007172NUP501
NM_001039752SLC22A101
NM_005971FXYD31
NM_021910FXYD31
NM_178566ZDHHC211
NM_207318CXorf391
NM_006802SF3A31
NM_002497NEK21
NM_030639BHLHB91
NM_022736MFSD11
NM_019069WDR5B1
NM_0152971
NM_014314DDX581
NM_152723CCDC891
NM_013313YPEL11
NM_002061GCLM1
NM_014500HTATSF11
NM_022483C5orf281
NM_133493CD1091
NM_017859UCKL11
NM_198568GJB71
NM_020152C21orf71
NM_015269ZCCHC111
NM_001009881ZCCHC111
NM_001009882ZCCHC111
XM_001128468NSF1
NM_006178NSF1
NM_001077619LOC1378861
NM_002272KRT41
NM_145284FAM122B1
NM_018015CXorf571
NM_014479ADAMDEC11
NM_030625CXXC61
NM_001080391SP1001
NM_198486RPL7L11
NM_133638ADAMTS191
NM_001013693LDLRAD21
NM_001729BTC1
NM_021052HIST1H2AE1
NM_024583SCRN31
NM_001077196PDE11A1
NM_001077197PDE11A1
NM_016953PDE11A1
NM_022098XPNPEP31
NM_024422DSC21
NM_004949DSC21
NM_175061JAZF11
NM_006393NEBL1
NM_022140EPB41L4A1
NM_0010994041
NM_198056SCN5A1
NM_0010994051
NM_000335SCN5A1
NM_006959ZNF171
NM_152355ZNF4411
NM_004788UBE4A1
NM_015534ZZZ31
NM_001040458ARTS-11
NM_001360DHCR71
NM_0321731
NM_006915RP21
NM_015477SIN3A1
NM_032818C9orf1001
NM_002251KCNS11
NM_001039891ZNF6741
NM_130385MRVI11
NM_0011001631
NM_0010985791
NM_0011001671
NM_003747TNKS1
XM_001134027LOC7318891
XM_001134374LOC7301321
NM_003417ZNF2641
NM_003161RPS6KB11
NM_020420DAZ41
NM_001005375DAZ41
NM_004081DAZ11
NM_001005786DAZ21
NM_020364DAZ31
NM_020363DAZ21
NM_001005785DAZ21
NM_205848SYT61
NM_199186BPGM1
NM_001724BPGM1
NM_022789IL251
NM_172314IL251
NM_017563IL17RD1
NM_001080973IL17RD1
NM_003558PIP5K1B1
NM_032814TMEM1181
NM_0011099031
NM_178558ZNF6801
NM_015578LSM14A1
NM_145055C18orf251
NM_001008239C18orf251
NM_139062CSNK1D1
NM_001893CSNK1D1
NM_006912RIT11
NM_145176SLC2A121
NM_003629PIK3R31
NM_014056HIGD1A1
NM_0010996681
NM_0010996691
NM_004673ANGPTL11
NM_024594PANK31
XM_935369LOC6463291
XM_944654LOC6487161
XM_935966LOC6417981
NM_015635GAPVD11
NM_016027LACTB21
NM_004389CTNNA21
XM_927997LOC6433381
XM_938980LOC6433381
NM_015471NSL11
NM_001042549NSL11
NM_019022TXNDC101
NM_002224ITPR31
NM_0010986141
NM_031292PUS7L1
NM_0010986151
NM_000332ATXN11
NM_002454MTRR1
NM_024010MTRR1
NM_001556IKBKB1
NM_002731PRKACB1
NM_182948PRKACB1
NM_001941DSC31
NM_024423DSC31
NM_138428C1orf2121
NM_053277CLIC61
NM_152996ST6GALNAC31
NM_020379MAN1C11
NM_005694COX171
NM_021215C20orf771
NM_025146NAT131
NM_212559XKRX1
NM_145167PIGM1
NM_024921POF1B1
NM_001304CPD1
NM_054026CNOT71
NM_014765TOMM201
NM_003393WNT8B1
NM_006838METAP21
NM_015196KIAA09221
NM_001040214TCBA11
NM_198515C10orf961
NM_001039840CHIC11
NM_016652CRNKL11
NM_001017923C14orf281
NM_173638NBPF151
NM_0011026631
XM_001128986LOC7289801
NM_183372NBPF111
NM_032431SYVN11
NM_172230SYVN11
NM_002853RAD11
NM_133377RAD11
XM_938779LOC6539721
NM_007276CBX31
NM_016587CBX31
NM_024762ZNF5521
NM_006520DYNLT31
NM_002025AFF21
NM_001128AP1G11
NM_001030007AP1G11
NM_207647FSD1L1
XM_944029LOC6479791
XM_942780SYNPO21
NM_032861SERAC11
NM_001160APAF11
NM_181868APAF11
NM_181869APAF11
NM_013229APAF11
NM_181861APAF11
NM_017684VPS13C1
NM_020821VPS13C1
NM_001364DLG21
NM_015553PIP3-E1
NM_002748MAPK61
NM_014631SH3PXD2A1
NM_133633SYN31
NM_003490SYN31
NM_018270C20orf201
NM_012294RAPGEF51
NM_018945PDE7B1
NM_001008495TMEM641
NM_001001420SMAD51
NM_001001419SMAD51
NM_005903SMAD51
NM_015677SH3YL11
NM_001017992DKFZp686D09721
NM_022459XPO41
NM_033227TRIM231
NM_001656TRIM231
NM_033228TRIM231
NM_001013736FAM47C1
NM_0011046471
NM_018155SLC25A361
NM_016021UBE2J11
NM_0011026591
NM_018263ASXL21
NM_001025300RAB121
NM_012345NUFIP11
NM_015431TRIM581
NM_198450APOOL1
NM_018899PCDHAC21
NM_018900PCDHA11
NM_018902PCDHA111
NM_031849PCDHA61
NM_018905PCDHA21
NM_018904PCDHA131
NM_031860PCDHA101
NM_018907PCDHA41
NM_018909PCDHA61
NM_018910PCDHA71
NM_018911PCDHA81
NM_018906PCDHA31
NM_018903PCDHA121
NM_018898PCDHAC11
NM_031857PCDHA91
NM_031411PCDHA11
NM_018908PCDHA51
NM_018901PCDHA101
NM_032144RAB6C1
NM_198896RAB6A1
NM_002869RAB6A1
NM_014384ACAD81
NM_014828TOX41
NM_001076786QSER11
NM_031889ENAM1
NM_152305KTELC11
NM_007006NUDT211
NM_007195POLI1
XM_942240LOC6505571
NM_0010986241
NM_033290MID11
NM_000381MID11
NM_138426GLCCI11
NM_001032289SLC35A21
NM_001042498SLC35A21
NM_014930ZNF5101
NM_033020TRIM331
NM_015906TRIM331
NM_020390EIF5A21
NM_012249RHOQ1
NM_012158FBXL31
NM_005277GPM6A1
NM_201592GPM6A1
NM_201591GPM6A1
NM_203446SYNJ11
NM_003895SYNJ11
NM_197964HSPC2681
NM_0249361
NM_018302C4orf191
NM_0011046291
XM_001125827LOC7278201
NM_213651SLC25A241
NM_013386SLC25A241
NM_033437PDE5A1
NM_001083PDE5A1
NM_033430PDE5A1
NM_032177RNUXA1
NM_001146ANGPT11
NM_004226STK17B1
NM_015020PHLPPL1
NM_145005C9orf721
NM_016575NT5DC31
NM_152622MIER31
NM_002486NCBP11
NM_018141MRPS101
NM_006611KLRA11
NM_006306SMC1A1
NM_134265WSB11
NM_015626WSB11
NM_002356MARCKS1
NM_018981DNAJC101
NM_145307PLEKHK11
NM_024641MANEA1
XM_496115LOC4403381
XM_940659LOC4403381
NM_007007CPSF61
NM_004034ANXA71
NM_001156ANXA71
NM_001557IL8RB1
NM_138468ICA1L1
NM_004666VNN11
NM_022728NEUROD61
XM_932529LOC6449491
XM_943887LOC6449491
NM_001040146CTF81
NM_001039690CTF81
NM_001040144CTF81
NM_001879MASP11
NM_145277HFE21
NM_202004HFE21
NM_213652HFE21
NM_213653HFE21
NM_001012515FECH1
NM_000140FECH1
NM_004798KIF3B1
NM_153365TAPT11
NM_152695ZNF4491
NM_000963PTGS21
NM_019903ADD31
NM_016824ADD31
NM_001121ADD31
NM_0175521
NM_000314PTEN1
NM_014071NCOA61
XM_001126195LOC7279421
NM_006930SKP1A1
NM_030647JHDM1D1
NM_001005473PLCXD31
XM_936846FLJ140821
XM_496575FLJ140821
NM_001039709CBFA2T21
NM_001032999CBFA2T21
NM_005093CBFA2T21
NM_001024858SPTB1
NM_001012509SLC45A21
NM_002397MEF2C1

Immunostimulatory motif search results


UGUGU - Judge et al. Sequence-dependent stimulation of the mammalian innate immune response by synthetic siRNA. Nat Biotechnol. 2005 Apr;23(4):457-62. (PMID: 15778705)
No Immunostimulatory motif found (UGUGU).

GUCCUUCAA - Hornung et al. Sequence-specific potent induction of IFN-alpha by short interfering RNA in plasmacytoid dendritic cells through TLR7. Nat Med. 2005 Mar;11(3):263-70. Epub 2005 Feb 20. (PMID: 15723075)
No Immunostimulatory motif found (GUCCUUCAA).



Settings used for search

Scores were calculated using scoring scheme SS1
Using AVG scoring mode.

Hit scores

ParameterSpec. Score cut-offColourComment
Hit to same geneColourHits to the same accession or genes with 100 percent identity (i.e. likely transcript variants). It is advisable to always check the genes hit.
High scoring hit≥80ColourHits are very likely to cause an off-target effect
Medium scoring hit<80¸≥40ColourHits with a small chance of causing an off target effect.
Low scoring hitColourHits very unlikely to cause an off-target effect

The mismatches are colour coded based on the weighting of that position.

Cut-offColour
MatchColour
Wobble base pairs (G-U)Colour
Critical bases (none)Colour
0.6 bitsColour
0.4 bitsColour
0.2 bitsColour

About SpecificityServer

SpecificityServer is designed to help you identify potential non-specific matches to your siRNA. It incorporates the latest information about non-specific matches (sequence-specific only).

Designed by: Alistair Chalk, Quan Du, Zicai Liang and Erik L.L. Sonnhammer


For more details about the algorithm "siRNA specificity searching incorporating mismatch tolerance data" Bioinformatics (2008) 24 (10): 1316-1317