Automatic recommendation
OK - siRNA is specific for both sense and antisense (guide) strands
15 hits were found for AGAAGAACUCCCUCGCCUC (8 guide strand hits and 7 sense strand hits.)
Hits to Guide strand
| Accession | Gene | Start | End | Details of mismatches | Spec. Score | Mismatches | Contiguous | Jackson style specificity | Hits similar gene | HSP Score | HSP % Identity | HSP length | Gene Length | ||||||||||||||||||
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | |||||||||||||
| NM_006456 | ST6GALNAC2 | 650 | 668 | u | G | A | A | G | A | A | C | U | C | C | C | U | C | G | u | C | U | C | 71 | 2 | 14 | c | false | <90% | 1908 | ||
| NM_021830 | PEO1 | 2521 | 2539 | A | c | A | A | G | A | A | C | U | C | C | C | U | C | a | C | C | U | u | 58 | 3 | 12 | c | false | <90% | 3630 | ||
| NM_206999 | CNOT1 | 4271 | 4289 | A | G | A | A | G | A | A | C | U | C | C | C | U | C | c | C | a | U | C | 51 | 2 | 14 | c | false | <90% | 5019 | ||
| NM_016284 | CNOT1 | 4271 | 4289 | A | G | A | A | G | A | A | C | U | C | C | C | U | C | c | C | a | U | C | 51 | 2 | 14 | c | false | <90% | 8449 | ||
| NM_032126 | C1orf49 | 89 | 107 | u | G | A | A | G | A | A | C | a | C | C | C | U | g | G | C | C | U | C | 18 | 3 | 7 | c | false | <90% | 1048 | ||
| NM_032207 | C19orf44 | 2453 | 2471 | g | G | A | A | G | A | A | C | g | C | C | C | U | C | G | a | C | U | C | 11 | 3 | 7 | c | false | <90% | 3364 | ||
| XM_001127121 | LOC728298 | 315 | 333 | A | G | A | A | G | c | A | C | U | C | C | g | U | C | G | C | C | U | C | 2 | 2 | 7 | false | <90% | 1317 | |||
| NM_014727 | MLL4 | 2661 | 2679 | u | G | A | A | G | A | u | g | U | C | C | C | U | C | G | C | C | U | C | -30 | 3 | 11 | bc | false | <90% | 8469 | ||
Hits to Sense strand
| Accession | Gene | Start | End | Details of mismatches | Spec. Score | Mismatches | Contiguous | Jackson style specificity | Hits similar gene | HSP Score | HSP % Identity | HSP length | Gene Length | ||||||||||||||||||
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | |||||||||||||
| NM_012309 | SHANK2 | 3991 | 4009 | a | A | G | G | g | G | A | G | G | G | A | G | U | U | u | U | U | C | U | 21 | 3 | 9 | c | false | <90% | 8778 | ||
| NM_001079839 | OCIAD1 | 413 | 431 | G | A | G | G | C | G | A | c | G | G | A | G | U | g | C | U | U | C | U | 18 | 2 | 7 | c | false | <90% | 2306 | ||
| NM_001040627 | NCALD | 94 | 112 | G | A | G | G | C | G | A | G | u | G | A | u | U | U | C | U | U | C | U | 6 | 2 | 8 | false | <90% | 3586 | |||
| NM_001040629 | NCALD | 94 | 112 | G | A | G | G | C | G | A | G | u | G | A | u | U | U | C | U | U | C | U | 6 | 2 | 8 | false | <90% | 3642 | |||
| NM_001040626 | NCALD | 94 | 112 | G | A | G | G | C | G | A | G | u | G | A | u | U | U | C | U | U | C | U | 6 | 2 | 8 | false | <90% | 3673 | |||
| NM_006387 | CHERP | 2306 | 2324 | G | A | G | u | C | G | A | G | G | G | c | G | U | U | C | U | U | C | c | -3 | 3 | 7 | c | false | <90% | 4105 | ||
| NM_006819 | STIP1 | 2098 | 2116 | G | A | G | G | g | G | A | G | G | G | g | G | U | U | C | U | U | C | c | -17 | 3 | 7 | c | false | <90% | 2219 | ||
3 prime UTR Seed matches
Hits to same gene : 0
Hits to other genes : 4277
View matched accessions
3 prime seed match details
Immunostimulatory motif search results
UGUGU - Judge et al. Sequence-dependent stimulation of the mammalian innate immune response by synthetic siRNA. Nat Biotechnol. 2005 Apr;23(4):457-62. (PMID: 15778705)
No Immunostimulatory motif found (UGUGU).
GUCCUUCAA - Hornung et al. Sequence-specific potent induction of IFN-alpha by short interfering RNA in plasmacytoid dendritic cells through TLR7. Nat Med. 2005 Mar;11(3):263-70. Epub 2005 Feb 20. (PMID: 15723075)
No Immunostimulatory motif found (GUCCUUCAA).
Settings used for search
Scores were calculated using scoring scheme SS1
Using AVG scoring mode.
Hit scores
| Parameter | Spec. Score cut-off | Colour | Comment |
| Hit to same gene | Colour | Hits to the same accession or genes with 100 percent identity (i.e. likely transcript variants). It is advisable to always check the genes hit. | |
| High scoring hit | ≥80 | Colour | Hits are very likely to cause an off-target effect |
| Medium scoring hit | <80¸≥40 | Colour | Hits with a small chance of causing an off target effect. |
| Low scoring hit | Colour | Hits very unlikely to cause an off-target effect |
The mismatches are colour coded based on the weighting of that position.
| Cut-off | Colour |
| Match | Colour |
| Wobble base pairs (G-U) | Colour |
| Critical bases (none) | Colour |
| 0.6 bits | Colour |
| 0.4 bits | Colour |
| 0.2 bits | Colour |
SpecificityServer is designed to help you identify potential non-specific matches to your siRNA. It incorporates the latest information about non-specific matches (sequence-specific only).
Designed by: Alistair Chalk, Quan Du, Zicai Liang and Erik L.L. Sonnhammer