Automatic recommendation
OK - siRNA is specific for both sense and antisense (guide) strands
10 hits were found for GGCUGCGGACUGUUUGGAA (4 guide strand hits and 6 sense strand hits.)
Hits to Guide strand
| Accession | Gene | Start | End | Details of mismatches | Spec. Score | Mismatches | Contiguous | Jackson style specificity | Hits similar gene | HSP Score | HSP % Identity | HSP length | Gene Length | ||||||||||||||||||
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | |||||||||||||
| NM_001699 | AXL | 2741 | 2759 | G | c | C | U | G | C | G | G | A | C | U | G | U | c | U | G | G | A | u | 64 | 3 | 11 | c | false | <90% | 4987 | ||
| NM_021913 | AXL | 2768 | 2786 | G | c | C | U | G | C | G | G | A | C | U | G | U | c | U | G | G | A | u | 64 | 3 | 11 | c | false | <90% | 5014 | ||
| NM_005302 | GPR37 | 90 | 108 | u | G | C | U | u | C | G | G | A | C | U | G | U | U | U | G | c | A | A | 23 | 3 | 11 | c | false | <90% | 3058 | ||
| NM_002180 | IGHMBP2 | 1585 | 1603 | G | G | C | U | G | C | G | G | g | C | U | G | U | U | U | G | a | g | c | 8 | 4 | 8 | c | false | <90% | 3955 | ||
Hits to Sense strand
| Accession | Gene | Start | End | Details of mismatches | Spec. Score | Mismatches | Contiguous | Jackson style specificity | Hits similar gene | HSP Score | HSP % Identity | HSP length | Gene Length | ||||||||||||||||||
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | |||||||||||||
| NM_003137 | SRPK1 | 3033 | 3051 | U | U | C | C | A | A | A | C | A | G | U | C | C | c | C | u | G | C | u | 49 | 3 | 13 | c | false | <90% | 4361 | ||
| NM_052928 | SMYD4 | 3609 | 3627 | U | U | C | C | c | A | A | C | A | G | U | C | C | G | a | A | G | C | C | 23 | 2 | 9 | c | false | <90% | 4269 | ||
| NM_021155 | CD209 | 1139 | 1157 | c | U | g | C | A | A | A | a | A | G | U | C | C | G | C | A | G | C | C | 16 | 3 | 11 | bc | false | <90% | 4266 | ||
| XR_017962 | FLJ14107 | 1384 | 1402 | g | U | C | C | A | A | g | C | A | G | U | C | C | c | C | A | G | C | C | 12 | 3 | 6 | c | false | <90% | 1792 | ||
| XR_017858 | FLJ14107 | 1384 | 1402 | g | U | C | C | A | A | g | C | A | G | U | C | C | c | C | A | G | C | C | 12 | 3 | 6 | c | false | <90% | 1792 | ||
| NM_003688 | CASK | 1782 | 1800 | U | U | C | C | A | g | A | C | A | G | U | C | C | c | C | A | G | C | u | 12 | 3 | 7 | c | false | <90% | 3122 | ||
3 prime UTR Seed matches
Hits to same gene : 0
Hits to other genes : 949
View matched accessions
3 prime seed match details
Immunostimulatory motif search results
UGUGU - Judge et al. Sequence-dependent stimulation of the mammalian innate immune response by synthetic siRNA. Nat Biotechnol. 2005 Apr;23(4):457-62. (PMID: 15778705)
No Immunostimulatory motif found (UGUGU).
GUCCUUCAA - Hornung et al. Sequence-specific potent induction of IFN-alpha by short interfering RNA in plasmacytoid dendritic cells through TLR7. Nat Med. 2005 Mar;11(3):263-70. Epub 2005 Feb 20. (PMID: 15723075)
No Immunostimulatory motif found (GUCCUUCAA).
Settings used for search
Scores were calculated using scoring scheme SS1
Using AVG scoring mode.
Hit scores
| Parameter | Spec. Score cut-off | Colour | Comment |
| Hit to same gene | Colour | Hits to the same accession or genes with 100 percent identity (i.e. likely transcript variants). It is advisable to always check the genes hit. | |
| High scoring hit | ≥80 | Colour | Hits are very likely to cause an off-target effect |
| Medium scoring hit | <80¸≥40 | Colour | Hits with a small chance of causing an off target effect. |
| Low scoring hit | Colour | Hits very unlikely to cause an off-target effect |
The mismatches are colour coded based on the weighting of that position.
| Cut-off | Colour |
| Match | Colour |
| Wobble base pairs (G-U) | Colour |
| Critical bases (none) | Colour |
| 0.6 bits | Colour |
| 0.4 bits | Colour |
| 0.2 bits | Colour |
SpecificityServer is designed to help you identify potential non-specific matches to your siRNA. It incorporates the latest information about non-specific matches (sequence-specific only).
Designed by: Alistair Chalk, Quan Du, Zicai Liang and Erik L.L. Sonnhammer