Predicted Off-targets for siRNA "ccgaggucgaaacguacgu" Using siRNA SpecificityServer

Specificity Server [Result page v1.0]


SpecificityServer - An siRNA design program for determining siRNA specificity

Automatic recommendation
OK - siRNA is specific for both sense and antisense (guide) strands

No (unfiltered) hits were found for :CCGAGGUCGAAACGUACGU


3 prime UTR Seed matches

Hits to same gene : 0
Hits to other genes : 717
View matched accessions

3 prime seed match details

AccessionGene nameHits to this gene
NM_020956PRX4
NM_020462ERGIC13
NM_004614TK22
NM_021915ZNF692
NM_001795CDH52
NM_1523572
NM_0011006242
NM_001050SSTR22
NM_021201MS4A72
NM_206938MS4A72
NM_206940MS4A72
NM_206939MS4A72
NM_001632ALPP2
NM_032753RAXL12
NM_145799SEPT62
NM_015129SEPT62
NM_001004354MGC615982
NM_000278PAX22
NM_003989PAX22
NM_003990PAX22
NM_003987PAX22
NM_003988PAX22
NM_001035534FAM129B2
NM_022833FAM129B2
NM_172367TUSC52
NM_014732KIAA05132
NM_024301FKRP2
NM_001039885FKRP2
NM_004302ACVR1B2
NM_001116ADCY92
NM_018708FEM1A2
NM_001039693FLJ379532
NM_020803KLHL82
NM_006852TLK22
NM_0011127072
NM_003681PDXK1
NM_020738KIDINS2201
NM_001071TYMS1
XM_001129814LOC7292721
XM_496041LOC4402431
XM_001128563FLJ361311
NM_0010014131
XM_496037LOC4402331
XM_001134268LOC7300271
XM_208835LOC4403201
XM_928237LOC6452021
XM_001125730LOC7278321
NM_023002HAPLN41
NM_016531KLF31
NM_199126ZNF585A1
NM_152655ZNF585A1
NM_007245ATXN2L1
NM_002833PTPN91
XM_001128975PTPN91
NM_148414ATXN2L1
XM_943323LOC6467191
XM_933842LOC6467191
NM_016142HSD17B121
NM_004742MAGI11
NM_003421ZNF37A1
NM_001007094ZNF37A1
XM_001128041LOC2852051
XM_001128618LOC2852051
NM_170721MSI21
NM_020802KIAA13771
NM_173086KRT6C1
NM_153256C10orf471
NM_017413APLN1
NM_0010992941
XM_936510KIAA16441
NM_022912REEP11
NM_005197CHES11
NM_0010854711
NM_001077352RBM231
NM_018107RBM231
NM_001077351RBM231
XM_498479LOC4399621
NM_0011097601
NM_025161C17orf701
NM_005999TSNAX1
NM_018441PECR1
NM_017459MFAP21
NM_002403MFAP21
NM_001002034FAM109B1
NM_016201AMOTL21
NM_021020LZTS11
NM_003004SECTM11
NM_182799EGFLAM1
NM_182801EGFLAM1
NM_182798EGFLAM1
NM_003043SLC6A61
NM_018177N4BP21
NM_001039844ACBD71
NM_019059TOMM71
NM_025147COQ10B1
NM_152906C22orf251
NM_016442ARTS-11
NM_021945C6orf851
NM_138396MARCH91
NM_145912NFAM11
NM_003881WISP21
NM_152287ZNF2761
NM_016734PAX51
NM_014707HDAC91
NM_024602HECTD31
NM_1735691
NM_006544EXOC51
NM_033064ATCAY1
NM_024958NRSN21
XM_001129552LOC7291731
NM_003741CHRD1
NM_006454MXD41
NM_021961TEAD11
XM_376305DUB31
NM_004711SYNGR11
NM_0010996941
NM_001039886ZNF8081
NM_018688BIN31
NM_198458ZNF4971
NM_174953ATP2A31
NM_174956ATP2A31
NM_174954ATP2A31
NM_174957ATP2A31
NM_005173ATP2A31
NM_174955ATP2A31
NM_006040HS3ST41
XM_001132212HS3ST41
NM_001259CDK61
NM_000378WT11
NM_024424WT11
NM_024425WT11
NM_024426WT11
NM_007350PHLDA11
XM_934649C1orf341
XM_945958C1orf341
NM_0010981991
NM_005279GPR11
NM_015557CHD51
NM_005057RBBP51
NM_018891LAMC21
NM_153711FAM26E1
NM_133455EMID11
XM_001134396LOC7302541
XM_940667SDC31
NM_002073GNAZ1
NM_001009992ZNF6481
NM_001013649LOC3889691
NM_024592SRD5A2L1
NM_016407C20orf431
NM_001077523AP3D11
NM_003938AP3D11
NM_017626DNAJB121
NM_015082FSTL41
NM_003006SELPLG1
NM_001002762DNAJB121
NM_052839PANX21
NM_021258IL22RA11
NM_025201PLEKHQ11
NM_138350THAP31
NM_175747OLIG31
NM_003032ST6GAL11
NM_173217ST6GAL11
NM_173216ST6GAL11
NM_033315RASL10B1
NM_018715RCC21
NM_001042590C9orf1271
NM_001042589C9orf1271
NM_199367SPG71
NM_001040875GDAP11
NM_018972GDAP11
NM_018951HOXA101
NM_153715HOXA101
NM_012398PIP5K1C1
NM_004058CAPS1
NM_080590CAPS1
NM_005663WHSC21
NM_024757EHMT11
NM_005336HDLBP1
NM_203346HDLBP1
NM_012140SLC25A101
NM_024877CNTD21
NM_139174LOC1619311
NM_003223TFAP41
NM_144582TEX2611
NM_181050AXIN11
NM_003502AXIN11
NM_001005609EDA1
NM_001399EDA1
NM_031945TSPAN101
NM_015321CRTC11
NM_0010984821
NM_016540GPR831
NM_002832PTPN71
NM_080588PTPN71
NM_001006616TSPAN171
NM_207335KLHDC61
XM_001126137LOC2849521
XM_001129340LOC2849521
NM_006578GNB51
NM_016194GNB51
NM_006121KRT11
NM_138477CDAN11
XM_001129302CDAN11
NM_001626AKT21
NM_173566C22orf301
NM_0010988191
NM_0010988181
NM_000923PDE4C1
NM_033107GTPBP101
NM_001042717GTPBP101
NM_002911UPF11
NM_025159CXorf211
XM_001134321LOC7322061
NM_024511C4orf151
NM_001040441ZBTB81
XM_001124597LOC7304111
NM_152531C3orf211
NM_020394ZNF6951
NM_025045BAIAP2L21
NM_003204NFE2L11
NM_000441SLC26A41
NM_032336GINS41
NM_1528331
XM_934676LOC6449251
XM_942888LOC6449251
NM_138385TMEM1291
NM_014023WDR371
NM_006315PCGF31
NM_022062PKNOX21
NM_024082PRRG31
NM_003899ARHGEF71
NM_001008216GALE1
NM_000403GALE1
NM_003947KALRN1
NM_152734C6orf891
NM_0010982131
NM_0010982121
NM_000861HRH11
NM_0010982111
NM_002609PDGFRB1
NM_001543NDST11
NM_006395ATG71
NM_017456PSCD11
NM_004762PSCD11
NM_001037165FOXK11
NM_005969NAP1L41
NM_0208271
NM_144599NIPA11
XM_059166LOC1276021
XM_938194LOC1276021
NM_000748CHRNB21
NM_018208ETNK21
NM_173493PASD11
XM_936719LOC6476621
NM_001009934DNASE1L11
NM_001009932DNASE1L11
NM_006730DNASE1L11
NM_001009933DNASE1L11
NM_021633KLHL121
NM_001001684FLJ458311
NM_024910ZNF7671
NM_023008FLJ129491
NM_0011132391
NM_022740HIPK21
NM_001080421UNC13A1
NM_147192DMBX11
NM_172225DMBX11
XM_001127668LOC7281191
XM_001131429LOC7311181
NM_000693ALDH1A31
NM_001031680RUNX31
NM_004350RUNX31
XM_379543LOC4014421
XM_944991C14orf821
XM_498578C14orf821
XM_926071LOC6425921
XM_379194LOC4010681
NM_144671FAM109A1
NM_005343HRAS1
NM_176795HRAS1
NM_153033KCTD71
XM_001130020KIAA11471
XM_001130081KIAA11471
NM_001080392KIAA11471
NM_005897IPP1
NM_0011013871
NM_080656MGC130171
NM_138292ATM1
NM_000051ATM1
NM_003439ZKSCAN11
NM_006556PMVK1
NM_020699GATAD2B1
XM_001127419FLJ308511
XM_001130207FLJ308511
NM_001040710FLJ308511
NM_002151HPN1
NM_182983HPN1
NM_020918GPAM1
NM_003307TRPM21
NM_145279MOBKL2C1
NM_201403MOBKL2C1
NM_001759CCND21
NM_001720BMP8B1
NM_015259ICOSLG1
NM_031215CABLES21
NM_017853TXNL4B1
NM_001013642LOC3886101
NM_002528NTHL11
NM_053045MGC143271
NM_001007024GOSR11
NM_001007025GOSR11
NM_004871GOSR11
XM_001131828BBS51
XM_001131846BBS51
NM_152384BBS51
NM_032108SEMA6B1
NM_021149COTL11
NM_0011014041
NM_016196RBM191
XM_001134334RBM191
XM_001134172LOC7300341
XM_001133186LOC7313451
NM_001012427FOXP41
NM_138457FOXP41
NM_001012426FOXP41
NM_031309SCRT11
NM_014924KIAA08311
NM_012306FAIM21
NM_032536NTNG21
NM_032189ATP11A1
NM_015205ATP11A1
NM_0011004231
NM_0011004221
NM_0011004241
NM_015535LOC260101
XM_001131639LOC7320271
XM_001129491LOC7291511
NM_003936CDK5R21
NM_033274ADAM191
NM_014003DHX381
NM_173165NFATC31
NM_173163NFATC31
NM_004555NFATC31
NM_024667VPS37B1
NM_052840BRUNOL61
NM_004064CDKN1B1
XM_001132813LOC7287411
XM_001132780LOC7287341
NM_001003940BMF1
NM_001003943BMF1
NM_001003942BMF1
NM_033503BMF1
NM_152280SYT111
NM_032105PPP1R12B1
NM_002481PPP1R12B1
NM_152678FAM116A1
NM_013286RBM15B1
NM_005160ADRBK21
NM_175932PSMD131
NM_002817PSMD131
NM_001037131CENTG21
NM_014914CENTG21
XM_001127197LOC7280051
XM_001127140LOC7280051
XM_001127212LOC7280051
XM_001127181LOC7280051
XM_372194C1orf1701
XM_936696C1orf1701
NM_001918DBT1
NM_024866ADM21
NM_0207001
NM_006133DAGLA1
NM_152913TMEM1301
NM_004719SFRS2IP1
NM_013433TNPO21
NM_212503PCTK31
NM_002596PCTK31
NM_212502PCTK31
NM_002928RGS161
NM_001504CXCR31
NM_015471NSL11
NM_001042549NSL11
NM_152771C19orf341
NM_006991ZNF1971
NM_021012KCNJ121
NM_007211RASSF81
NM_003099SNX11
NM_152826SNX11
NM_148955SNX11
NM_152372MYOM31
NM_001031617COX191
NM_001319CSNK1G21
NM_003733OASL1
NM_198213OASL1
NM_148169FBXO171
NM_024907FBXO171
NM_001011708GTPBP91
NM_013341GTPBP91
NM_005036PPARA1
NM_001001928PPARA1
NM_080664C14orf1261
XM_943355LOC3889231
XM_373973LOC3889231
NM_003677DENR1
NM_001928CFD1
XM_937979MGC463361
XM_290712MGC463361
NM_018339RFK1
NM_0011004081
NM_001033046C17orf621
NM_0011004071
NM_000382ALDH3A21
NM_001031806ALDH3A21
NM_001030010ALDH3B11
NM_000694ALDH3B11
NM_019008SMCR7L1
NM_016094COMMD21
NM_031886KCNA71
XM_001129261LOC7290821
XM_001129056LOC7312731
NM_006903PPA21
NM_176866PPA21
NM_176869PPA21
NM_176867PPA21
NM_001034191PPA21
NM_032796SYAP11
XM_001132728LOC7323141
XM_001128520LOC7287991
NM_006952UPK1B1
NM_0324531
NM_019067GNL3L1
NM_001003941OGDH1
NM_153687IKIP1
NM_000139MS4A21
NM_007110TEP11
XM_001132559LOC7322901
XM_001128665LOC7288691
NM_003644GAS71
NM_201432GAS71
NM_201433GAS71
NM_017856GEMIN81
NM_001042479GEMIN81
NM_001042480GEMIN81
NM_015431TRIM581
NM_002061GCLM1
NM_023110FGFR11
NM_023106FGFR11
NM_015850FGFR11
NM_023111FGFR11
NM_023105FGFR11
NM_006139CD281
NM_001099ACPP1
NM_002874RAD23B1
NM_018243SEPT111
NM_018168C14orf1051
NM_014829DDX461
NM_015352POFUT11
NM_145111C7orf381
NM_004729ZBED11
NM_016183MRTO41
NM_003950F2RL31
NM_198276TMEM171
NM_032374C14orf1531
NM_000952PTAFR1
NM_015174KIAA10861
NM_203395IYD1
NM_207381TNFAIP8L31
NM_002105H2AFX1
XM_001132180LOC2554801
XM_001134335LOC2554801
NM_005440RND21
NM_017861PIGX1
XM_938853LOC2854841
XM_376303LOC2854841
NM_002853RAD11
NM_133377RAD11
NM_012232PTRF1
NM_144775SMCR81
NM_024068OBFC2B1
NM_016520C9orf781
NM_018128TSR11
NM_152263TPM31
NM_003082SNAPC11
NM_022098XPNPEP31
NM_152356ZNF4911
NM_001031683IFIT31
NM_001549IFIT31
NM_203307ZNF3211
XM_001129292MBOAT21
NM_138799MBOAT21
NM_000961PTGIS1
NM_145341PDCD41
NM_014456PDCD41
NM_207366FLJ440601
NM_182594ZNF4541
XM_001126170LOC7305191
XM_001132260LOC7300601
NM_213597KRBA21
NM_004936CDKN2B1
NM_078487CDKN2B1
NM_0010986271
NM_032643IRF51
NM_002200IRF51
NM_0010986311
NM_0010986291
NM_0010986281
NM_0010986301
NM_005052RAC31
NM_198076FAM36A1
NM_020860STIM21
NM_002636PHF11
NM_178520TMEM1051
NM_148960CLDN191
NM_004697PRPF41
XM_931934LOC6531511
XM_001129082LOC6531511
NM_032569N-PAC1
NM_198887NUP431
NM_198271LMOD31
NM_001018116LOC3472731
NM_207351PRRT31
NM_004798KIF3B1
NM_021818SAV11
XM_925853LOC6530531
NM_024646ZYG11B1
NM_024345WDR321
NM_015621CCDC691
NM_001001479SLC35E41
NM_023923PHACTR41
NM_001048183PHACTR41
XM_001124844LOC7304201
NM_032967PCDH11X1
NM_000272NPHP11
XM_379100LOC4009581
XM_945436LOC4009581
XM_209363SIRPB21
XM_939897SIRPB21
NM_144984C10orf721
NM_016546C1RL1
NM_198196CD961
NM_005816CD961
NM_001047605KIR3DX11
NM_018225SMU11
NM_014911AAK11
NM_138809CMBL1
NM_015154MESDC21
NM_005759ABI21
XM_001126750ABI21
NM_016422RNF1411
NM_005431XRCC21
NM_173542LOC1964631
NM_174929ZMIZ21
NM_031449ZMIZ21
NM_031904FKSG441
NM_1334471
NM_001001694FLJ419931
NM_152437ZNF6641
XM_938689KIAA16711
XM_371461KIAA16711
NM_001040439MAPK8IP31
NM_015133MAPK8IP31
NM_020935USP371
NM_152450FAM81A1
NM_021706LAIR11
NM_002287LAIR11
NM_000883IMPDH11
NM_0011026051
NM_183243IMPDH11
NM_175744RHOC1
NM_001042679RHOC1
NM_001042678RHOC1
NM_001692ATP6V1B11
NM_016612SLC25A371
NM_006698BLCAP1
NM_007129ZIC21
NM_0306321
NM_175857KRTAP8-11
NM_148964CTSE1
NM_001910CTSE1
NM_001029882AHDC11
XM_928199LOC4005811
NM_006770MARCO1
NM_017491WDR11
NM_005112WDR11
NM_014967MTMR151
NM_000046ARSB1
NM_002999SDC41
NM_024104C19orf421
NM_005883APC21
XM_942689LOC6427401
NM_001014283DCUN1D21
NM_014460CSDC21
NM_000683ADRA2C1
NM_0321731
NM_003983SLC7A61
NM_001076785SLC7A61
NM_001023587ABCC51
NM_000742CHRNA21
XM_939123LOC4014971
XM_376822LOC4014971
NM_014850SRGAP31
NM_001033117SRGAP31
NM_000875IGF1R1
NM_015898ZBTB7A1
NM_172169CAMK2G1
NM_001222CAMK2G1
NM_172173CAMK2G1
NM_172171CAMK2G1
NM_172170CAMK2G1
XM_378787LOC2843951
XM_943993LOC2843951
NM_002857PEX191
NM_170606MLL31
NM_024094DCC11
NM_144997FLCN1
NM_020165RAD181
NM_015545PTCD11
NM_003190TAPBP1
NM_018260ZNF7011
NM_022479WBSCR171
NM_007357COG21
NM_015033FNBP11
NM_031476CRISPLD21
NM_206853QKI1
NM_206854QKI1
NM_206855QKI1
NM_017610RNF1111
NM_000446PON11
NM_152253CHKB1
NM_005134PPP4R11
NM_001042388PPP4R11
NM_005715UST1
NM_001032374ZNF2261
NM_015919ZNF2261
NM_001032375ZNF2261
NM_031845MAP21
NM_031847MAP21
NM_001039538MAP21
NM_002374MAP21
NM_0010988141
NM_002332LRP11
NM_001002862DERL31
NM_198440DERL31
NM_003724JRK1
NM_001025266LOC2853821
NM_013302EEF2K1
XM_001134399LOC7302571
NM_016308CMPK1
NM_002518NPAS21
NM_003407ZFP361
NM_013248NXT11
NM_025219DNAJC51
NM_005831CALCOCO21
NM_032932RAB11FIP41
NM_005432XRCC31
NM_0011001191
NM_0011001181
NM_003777DNAH111
NM_020117LARS1
NM_001007559SS181
NM_005637SS181
NM_005937MLLT61
NM_144686TMC41
XM_927238LOC6439881
XM_936418LOC6473471
NM_024541C10orf761
NM_006750SNTB21
NM_024830AYTL21
NM_001013693LDLRAD21
NM_006595API51
NM_152396METTL61
NM_152942TNFRSF81
NM_001243TNFRSF81
NM_014665LRRC141
NM_0010992871
NM_001012418LOC3401561
NM_003382VIPR21
NM_182832PLAC41
NM_012193FZD41
NM_181706DPH41
NM_006640SEPT91
NM_172097CATSPER21
NM_017921NPLOC41
XM_001129120LOC7290341
NM_015151DIP2A1
NM_006789APOBEC21
NM_002919RFX31
NM_178003PPP2R41
NM_021131PPP2R41
NM_178000PPP2R41
NM_178001PPP2R41
NM_000427LOR1
NM_006753SURF61
NM_001093ACACB1
NM_001033925TIAL11
NM_003252TIAL11
NM_006076HRBL1
NM_003108SOX111
NM_021977SLC22A31
NM_001440EXTL31
NM_001500GMDS1
NM_001003682TTMB1
NM_001039523CHRNA11
NM_000079CHRNA11
NM_172107KCNQ21
NM_172106KCNQ21
NM_172108KCNQ21
NM_004518KCNQ21
NM_032590FBXL101
NM_001005366FBXL101
XM_941783JMJD31
NM_001080424JMJD31
XM_001132645ODZ31
XM_931600ODZ31
NM_001080477ODZ31
XM_940722ODZ31
NM_004859CLTC1

Immunostimulatory motif search results


UGUGU - Judge et al. Sequence-dependent stimulation of the mammalian innate immune response by synthetic siRNA. Nat Biotechnol. 2005 Apr;23(4):457-62. (PMID: 15778705)
No Immunostimulatory motif found (UGUGU).

GUCCUUCAA - Hornung et al. Sequence-specific potent induction of IFN-alpha by short interfering RNA in plasmacytoid dendritic cells through TLR7. Nat Med. 2005 Mar;11(3):263-70. Epub 2005 Feb 20. (PMID: 15723075)
No Immunostimulatory motif found (GUCCUUCAA).



Settings used for search

Scores were calculated using scoring scheme SS1
Using AVG scoring mode.

Hit scores

ParameterSpec. Score cut-offColourComment
Hit to same geneColourHits to the same accession or genes with 100 percent identity (i.e. likely transcript variants). It is advisable to always check the genes hit.
High scoring hit≥80ColourHits are very likely to cause an off-target effect
Medium scoring hit<80¸≥40ColourHits with a small chance of causing an off target effect.
Low scoring hitColourHits very unlikely to cause an off-target effect

The mismatches are colour coded based on the weighting of that position.

Cut-offColour
MatchColour
Wobble base pairs (G-U)Colour
Critical bases (none)Colour
0.6 bitsColour
0.4 bitsColour
0.2 bitsColour

About SpecificityServer

SpecificityServer is designed to help you identify potential non-specific matches to your siRNA. It incorporates the latest information about non-specific matches (sequence-specific only).

Designed by: Alistair Chalk, Quan Du, Zicai Liang and Erik L.L. Sonnhammer


For more details about the algorithm "siRNA specificity searching incorporating mismatch tolerance data" Bioinformatics (2008) 24 (10): 1316-1317