Automatic recommendation
OK - siRNA is specific for both sense and antisense (guide) strands
11 hits were found for GGUUUGUAUUAGUAGUUGA (4 guide strand hits and 7 sense strand hits.)
Hits to Guide strand
| Accession | Gene | Start | End | Details of mismatches | Spec. Score | Mismatches | Contiguous | Jackson style specificity | Hits similar gene | HSP Score | HSP % Identity | HSP length | Gene Length | ||||||||||||||||||
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | |||||||||||||
| NM_001031739 | ASB9 | 1344 | 1362 | c | G | U | U | U | G | U | A | U | U | A | u | U | A | G | U | U | a | g | 56 | 4 | 10 | false | <90% | 1665 | |||
| NM_024087 | ASB9 | 1345 | 1363 | c | G | U | U | U | G | U | A | U | U | A | u | U | A | G | U | U | a | g | 56 | 4 | 10 | false | <90% | 1667 | |||
| NM_001040285 | PAPD5 | 6074 | 6092 | c | a | U | U | U | u | U | A | U | U | A | G | U | A | G | U | U | G | c | 18 | 4 | 12 | bc | false | <90% | 7448 | ||
| NM_001040284 | PAPD5 | 6215 | 6233 | c | a | U | U | U | u | U | A | U | U | A | G | U | A | G | U | U | G | c | 18 | 4 | 12 | bc | false | <90% | 7589 | ||
Hits to Sense strand
| Accession | Gene | Start | End | Details of mismatches | Spec. Score | Mismatches | Contiguous | Jackson style specificity | Hits similar gene | HSP Score | HSP % Identity | HSP length | Gene Length | ||||||||||||||||||
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | |||||||||||||
| NM_024690 | MUC16 | 6835 | 6853 | c | C | A | A | C | U | A | C | U | A | A | U | A | C | A | g | A | a | a | 64 | 4 | 14 | c | false | <90% | 43816 | ||
| NM_014048 | MKL2 | 7758 | 7776 | c | C | A | A | C | U | A | C | U | A | A | g | A | C | A | A | A | g | C | 61 | 3 | 10 | false | <90% | 8680 | |||
| NM_018164 | C12orf11 | 976 | 994 | U | C | A | A | C | U | A | C | U | A | u | U | A | C | A | A | A | u | a | 31 | 3 | 10 | c | false | <90% | 2635 | ||
| NM_018656 | SLC35E3 | 1362 | 1380 | U | C | A | A | C | U | A | a | U | A | A | U | A | C | A | A | A | a | u | 30 | 3 | 9 | c | false | <90% | 2347 | ||
| NM_152470 | RNF165 | 5099 | 5117 | a | C | A | A | C | U | A | C | g | A | A | U | A | C | A | A | A | a | a | 30 | 4 | 8 | c | false | <90% | 5276 | ||
| NM_015849 | ELA2B | 755 | 773 | g | C | A | A | C | U | A | C | U | A | c | U | A | C | A | A | g | C | C | 13 | 3 | 9 | c | false | <90% | 941 | ||
| NM_014892 | RBM16 | 1133 | 1151 | U | C | A | A | C | a | A | u | U | A | A | U | A | C | A | A | A | C | C | -25 | 2 | 11 | bc | false | <90% | 5018 | ||
3 prime UTR Seed matches
Hits to same gene : 0
Hits to other genes : 2337
View matched accessions
3 prime seed match details
Immunostimulatory motif search results
UGUGU - Judge et al. Sequence-dependent stimulation of the mammalian innate immune response by synthetic siRNA. Nat Biotechnol. 2005 Apr;23(4):457-62. (PMID: 15778705)
No Immunostimulatory motif found (UGUGU).
GUCCUUCAA - Hornung et al. Sequence-specific potent induction of IFN-alpha by short interfering RNA in plasmacytoid dendritic cells through TLR7. Nat Med. 2005 Mar;11(3):263-70. Epub 2005 Feb 20. (PMID: 15723075)
No Immunostimulatory motif found (GUCCUUCAA).
Settings used for search
Scores were calculated using scoring scheme SS1
Using AVG scoring mode.
Hit scores
| Parameter | Spec. Score cut-off | Colour | Comment |
| Hit to same gene | Colour | Hits to the same accession or genes with 100 percent identity (i.e. likely transcript variants). It is advisable to always check the genes hit. | |
| High scoring hit | ≥80 | Colour | Hits are very likely to cause an off-target effect |
| Medium scoring hit | <80¸≥40 | Colour | Hits with a small chance of causing an off target effect. |
| Low scoring hit | Colour | Hits very unlikely to cause an off-target effect |
The mismatches are colour coded based on the weighting of that position.
| Cut-off | Colour |
| Match | Colour |
| Wobble base pairs (G-U) | Colour |
| Critical bases (none) | Colour |
| 0.6 bits | Colour |
| 0.4 bits | Colour |
| 0.2 bits | Colour |
SpecificityServer is designed to help you identify potential non-specific matches to your siRNA. It incorporates the latest information about non-specific matches (sequence-specific only).
Designed by: Alistair Chalk, Quan Du, Zicai Liang and Erik L.L. Sonnhammer