Automatic recommendation
OK - siRNA is specific for both sense and antisense (guide) strands
10 hits were found for GGAUCAGCAUGACAGUAAC (2 guide strand hits and 8 sense strand hits.)
Hits to Guide strand
| Accession | Gene | Start | End | Details of mismatches | Spec. Score | Mismatches | Contiguous | Jackson style specificity | Hits similar gene | HSP Score | HSP % Identity | HSP length | Gene Length | ||||||||||||||||||
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | |||||||||||||
| NM_003184 | TAF2 | 3774 | 3792 | G | c | A | U | a | A | G | C | A | U | G | A | C | A | G | U | A | A | a | 32 | 3 | 13 | bc | false | <90% | 5049 | ||
| NM_003409 | ZFP161 | 570 | 588 | G | G | A | U | C | A | G | g | A | U | G | A | C | A | G | U | c | c | u | 6 | 4 | 8 | c | false | <90% | 2896 | ||
Hits to Sense strand
| Accession | Gene | Start | End | Details of mismatches | Spec. Score | Mismatches | Contiguous | Jackson style specificity | Hits similar gene | HSP Score | HSP % Identity | HSP length | Gene Length | ||||||||||||||||||
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | |||||||||||||
| NM_016388 | TRAT1 | 22 | 40 | u | U | U | A | C | U | G | U | C | A | U | G | C | U | G | c | U | u | u | 64 | 4 | 14 | c | false | <90% | 1695 | ||
| NM_017824 | MARCH5 | 777 | 795 | a | U | U | c | C | U | G | U | C | A | U | G | C | U | G | A | U | a | u | 53 | 4 | 13 | c | false | <90% | 3941 | ||
| XR_016885 | LOC645163 | 1096 | 1114 | a | U | U | A | C | U | G | U | C | A | U | G | C | U | G | g | g | u | C | 45 | 4 | 14 | c | false | <90% | 1291 | ||
| XR_018802 | LOC650032 | 1096 | 1114 | a | U | U | A | C | U | G | U | C | A | U | G | C | U | G | g | g | u | C | 45 | 4 | 14 | c | false | <90% | 1291 | ||
| NM_015084 | MRPS27 | 146 | 164 | a | U | U | A | C | U | G | U | C | u | U | G | C | U | G | A | U | C | u | 10 | 3 | 8 | bc | false | <90% | 2631 | ||
| XM_001133506 | LOC729534 | 208 | 226 | G | U | U | A | C | U | c | U | C | A | U | G | C | U | G | u | U | C | u | 2 | 3 | 8 | c | false | <90% | 878 | ||
| XM_001133339 | LOC731569 | 208 | 226 | G | U | U | A | C | U | c | U | C | A | U | G | C | U | G | u | U | C | u | 2 | 3 | 8 | c | false | <90% | 878 | ||
| NM_177986 | DSG4 | 2866 | 2884 | c | U | U | A | C | g | G | U | a | A | U | G | C | U | G | A | U | C | C | -25 | 3 | 10 | bc | false | <90% | 3579 | ||
3 prime UTR Seed matches
Hits to same gene : 0
Hits to other genes : 1370
View matched accessions
3 prime seed match details
Immunostimulatory motif search results
UGUGU - Judge et al. Sequence-dependent stimulation of the mammalian innate immune response by synthetic siRNA. Nat Biotechnol. 2005 Apr;23(4):457-62. (PMID: 15778705)
No Immunostimulatory motif found (UGUGU).
GUCCUUCAA - Hornung et al. Sequence-specific potent induction of IFN-alpha by short interfering RNA in plasmacytoid dendritic cells through TLR7. Nat Med. 2005 Mar;11(3):263-70. Epub 2005 Feb 20. (PMID: 15723075)
No Immunostimulatory motif found (GUCCUUCAA).
Settings used for search
Scores were calculated using scoring scheme SS1
Using AVG scoring mode.
Hit scores
| Parameter | Spec. Score cut-off | Colour | Comment |
| Hit to same gene | Colour | Hits to the same accession or genes with 100 percent identity (i.e. likely transcript variants). It is advisable to always check the genes hit. | |
| High scoring hit | ≥80 | Colour | Hits are very likely to cause an off-target effect |
| Medium scoring hit | <80¸≥40 | Colour | Hits with a small chance of causing an off target effect. |
| Low scoring hit | Colour | Hits very unlikely to cause an off-target effect |
The mismatches are colour coded based on the weighting of that position.
| Cut-off | Colour |
| Match | Colour |
| Wobble base pairs (G-U) | Colour |
| Critical bases (none) | Colour |
| 0.6 bits | Colour |
| 0.4 bits | Colour |
| 0.2 bits | Colour |
SpecificityServer is designed to help you identify potential non-specific matches to your siRNA. It incorporates the latest information about non-specific matches (sequence-specific only).
Designed by: Alistair Chalk, Quan Du, Zicai Liang and Erik L.L. Sonnhammer