Predicted Off-targets for siRNA "gccaaucccguugaaauuc" Using siRNA SpecificityServer

Specificity Server [Result page v1.0]


SpecificityServer - An siRNA design program for determining siRNA specificity

Automatic recommendation
OK - siRNA is specific for both sense and antisense (guide) strands

14 hits were found for GCCAAUCCCGUUGAAAUUC (9 guide strand hits and 5 sense strand hits.)

Hits to Guide strand

AccessionGeneStartEndDetails of mismatchesSpec.
Score
MismatchesContiguousJackson style
specificity
Hits similar
gene
HSP
Score
HSP %
Identity
HSP lengthGene Length
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19
NM_015608 C10orf137 2087 2105 uCCAAUCCCGUUaAAAUau79 4 11   false   <90%   4596
NM_004360 CDH1 2528 2546 GCCAAUCCCGaUGAAAUUg33 2 10 c false   <90%   4828
XM_001129001 LOC728989 1598 1616 aCCAAUuCuGUUGAAAUUC-28 3 10 bc false   <90%   1876
XM_001133122 LOC728920 1748 1766 aCCAAUuCuGUUGAAAUUC-28 3 10 bc false   <90%   2026
XM_001132893 LOC728802 1875 1893 aCCAAUuCuGUUGAAAUUC-28 3 10 bc false   <90%   2153
XM_001132890 LOC728802 1891 1909 aCCAAUuCuGUUGAAAUUC-28 3 10 bc false   <90%   2169
XM_001133119 LOC728920 1891 1909 aCCAAUuCuGUUGAAAUUC-28 3 10 bc false   <90%   2169
XM_001132896 LOC728802 2074 2092 aCCAAUuCuGUUGAAAUUC-28 3 10 bc false   <90%   2352
XM_001133124 LOC728920 2074 2092 aCCAAUuCuGUUGAAAUUC-28 3 10 bc false   <90%   2352


Hits to Sense strand






AccessionGeneStartEndDetails of mismatchesSpec.
Score
MismatchesContiguousJackson style
specificity
Hits similar
gene
HSP
Score
HSP %
Identity
HSP lengthGene Length
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19
XR_015713 LOC732086 733 751 GAcUUUCAACGGGAUUaug49 4 13 c false   <90%   1264
XR_015726 LOC729954 733 751 GAcUUUCAACGGGAUUaug49 4 13 c false   <90%   1264
NM_001045 SLC6A4 773 791 GAuUUUCAAaGGGAUUGGu-7 3 8 bc false   <90%   2775
NM_005858 AKAP8 1451 1469 uccUUUCAAaGGGAUUGGC-15 4 9 bc false   <90%   3545
NM_182758 WDR72 2690 2708 GAAUUcCAAgGGGAUUGGa-51 3 8 bc false   <90%   5900


3 prime UTR Seed matches

Hits to same gene : 0
Hits to other genes : 842
View matched accessions

3 prime seed match details

AccessionGene nameHits to this gene
NM_018273TMEM1433
NM_013443ST6GALNAC63
NM_030641APOL62
NM_001654ARAF2
NM_014521SH3BP42
NM_133265AMOT2
NM_006927ST3GAL22
NM_052936ATG4A2
NM_178270ATG4A2
XM_001129929LOC7283392
XM_001133196LOC7315072
NM_004584RAD9A2
NM_004946DOCK22
NM_004744LRAT2
NM_001005732C21orf342
NM_001005733C21orf342
NM_198403MMD22
NM_0011006002
NM_015520MAGI12
NM_001033057MAGI12
NM_017801CMTM62
NM_003505FZD12
NM_0010986322
NM_032375AKT1S12
NM_0010986332
NM_001040647RCP92
NM_001040648RCP92
NM_014478RCP92
XM_943278LOC6447272
XM_932334LOC6447272
NM_006206PDGFRA2
NM_001859SLC31A11
NM_178011LRRTM31
NM_022351EFCBP11
NM_153025C16orf551
NM_0011001701
NM_0011001691
NM_006839IMMT1
NM_021033RAP2A1
NM_080654CCDC341
NM_007050PTPRT1
NM_133170PTPRT1
NM_173517VKORC1L11
NM_001503GPLD11
NM_018328MBD51
NM_001014342FLG21
NM_003506FZD61
NM_014282HABP41
NM_031924RSHL21
NM_004588SCN2B1
NM_002668PLP21
NM_139135ARID1A1
NM_006015ARID1A1
NM_0207001
NM_015545PTCD11
XM_940667SDC31
NM_152649MLKL1
NM_004229CRSP21
NM_145295ZNF6271
NM_001110ADAM101
NM_001009998SSBP41
NM_032627SSBP41
NM_004624VIPR11
NM_014848SV2B1
NM_002119HLA-DOA1
NM_014867KBTBD111
NM_018658KCNJ161
NM_170742KCNJ161
NM_170741KCNJ161
NM_016952CDON1
NM_004798KIF3B1
NM_002298LCP11
NM_152470RNF1651
NM_016441CRIM11
NM_002364MAGEB21
NM_000271NPC11
NM_030891LRRC31
XM_001130699LOC7317611
XM_001129872LOC7292961
NM_004455EXTL11
NM_152643KNDC11
NM_021642FCGR2A1
NM_201563FCGR2C1
NM_001005411FCGR2C1
NM_001005410FCGR2C1
NM_033437PDE5A1
NM_001083PDE5A1
NM_033430PDE5A1
NM_005399PRKAB21
NM_001008406BCAP291
NM_006212PFKFB21
NM_018899PCDHAC21
NM_018900PCDHA11
NM_018902PCDHA111
NM_031849PCDHA61
NM_018905PCDHA21
NM_018904PCDHA131
NM_031860PCDHA101
NM_018907PCDHA41
NM_018909PCDHA61
NM_018910PCDHA71
NM_018911PCDHA81
NM_018906PCDHA31
NM_018903PCDHA121
NM_018898PCDHAC11
NM_031857PCDHA91
NM_031411PCDHA11
NM_018908PCDHA51
NM_018901PCDHA101
NM_025230WDR231
NM_181357WDR231
NM_001039613IAH11
NM_018035FLJ102411
NM_003760EIF4G31
XM_001132729LOC7290111
XM_001131485LOC7309811
NM_017594DIRAS21
NM_003478CUL51
NM_133646ZAK1
NM_001009584ARMCX61
NM_019007ARMCX61
NM_003706PLA2G4C1
NM_198887NUP431
NM_138727ST7L1
NM_138728ST7L1
NM_017744ST7L1
NM_020223FAM20C1
XM_001134390LOC7301791
XM_001134142LOC7320301
XM_001134423C9orf1441
NM_003558PIP5K1B1
NM_003180SYT51
NM_022349MS4A6A1
NM_001241CCNT21
NM_015274MAN2B21
NM_015358MORC31
NM_032287LDOC1L1
NM_001077418FLJ221671
NM_001077416FLJ221671
NM_001077419FLJ221671
NM_004947DOCK31
NM_152860SP71
NM_207387C17orf761
XM_930431LOC6451951
XM_942574LOC6451951
NM_024312GNPTAB1
NM_007006NUDT211
NM_203418DSCR11
NM_203417DSCR11
NM_004414DSCR11
NM_032294CAMKK11
NM_172206CAMKK11
NM_002645PIK3C2A1
NM_203447DOCK81
NM_020689SLC24A31
NM_024677NSUN71
NM_002589PCDH71
NM_015296DOCK91
NM_148962OXER11
NM_001080415SR1401
XM_940802SR1401
NM_014877HELZ1
NM_206893MS4A101
NM_001080529WIPF31
XM_939315LOC6441501
XM_935705LOC6441501
NM_018684KIAA11661
NM_001001995GPM6B1
NM_006496GNAI31
NM_024638QTRTD11
NM_181500CUTL11
NM_001913CUTL11
NM_144991C21orf291
NM_002310LIFR1
NM_022841RFXDC21
NM_004085TIMM8A1
NM_001248ENTPD31
NM_001040441ZBTB81
XM_001124597LOC7304111
NM_006936SUMO31
NM_024921POF1B1
NM_145278C1orf1501
NM_004319ASTN11
NM_145807LOC1261471
NM_001453FOXC11
NM_020774MIB11
NM_001010922C1orf1781
NM_018195C11orf571
NM_001082969C11orf571
NM_001082970C11orf571
NM_019086FLJ206741
NM_004736XPR11
NM_015064ERC11
NM_178038ERC11
NM_178037ERC11
NM_000878IL2RB1
NM_022744C16orf581
NM_030918SNX271
NM_001080480MBOAT11
XM_001131050MBOAT11
XM_001131044MBOAT11
NM_018129PNPO1
NM_006486FBLN11
NM_018347C20orf291
NM_152320ZNF6411
NM_173728ARHGEF151
NM_015072TTLL51
NM_000474TWIST11
NM_020126SPHK21
NM_032414PROK11
NM_021706LAIR11
NM_002287LAIR11
NM_006517SLC16A21
NM_002271RANBP51
NM_207391RGS9BP1
NM_001039705TRO1
NM_007118TRIO1
NM_006897HOXC91
NM_001259CDK61
NM_019008SMCR7L1
NM_181337KAAG11
NM_001304CPD1
NM_006377UNC13B1
NM_001025356TMEM16F1
NM_019887DIABLO1
NM_138929DIABLO1
NM_138930DIABLO1
NM_006255PRKCH1
NM_0011113191
NM_024539RNF1281
NM_194463RNF1281
NM_020128MDM11
NM_020661AICDA1
NM_012465TLL21
NM_001295CCR11
NM_001010870TDRD61
NM_014238KSR11
NM_006056NMUR11
NM_139157ST51
NM_005418ST51
NM_213618ST51
NM_003410ZFX1
NM_032679ZNF5771
NM_024887DHDDS1
NM_205861DHDDS1
NM_001042601TTC141
NM_001011713NAT121
NM_004852ONECUT21
NM_000346SOX91
NM_0010999521
NM_002222ITPR11
NM_053041COMMD71
NM_0010993391
NM_0011024211
NM_0011024201
NM_006007ZFAND51
NM_152230IPMK1
NM_001014797KCNMA11
NM_003507FZD71
NM_020782KLHDC51
NM_133490KCNG41
NM_033224PURB1
NM_178820FBXO271
NM_181784SPRED21
NM_014211GABRP1
NM_017778WHSC1L11
NM_017749FLJ202941
NM_014388C1orf1071
NM_014590ERVWE11
NM_152478ZNF5831
NM_018136ASPM1
NM_001031719DHRS121
NM_005076CNTN21
NM_177452TRAPPC6B1
NM_001079537TRAPPC6B1
NM_0011003981
NM_015085GARNL41
NM_001754RUNX11
NM_001001890RUNX11
NM_005095ZMYM41
NM_134325SLC26A91
NM_052934SLC26A91
NM_152373ZNF6841
NM_007285GABARAPL21
NM_198715PTGER31
NM_080669SLC46A11
NM_183381RNF131
NM_183384RNF131
NM_007282RNF131
NM_183383RNF131
NM_145016GLYATL21
NM_015185ARHGEF91
NM_173598KSR21
NM_007283MGLL1
NM_001003794MGLL1
NM_000831GRIK31
NM_207578PRKACB1
NM_001012993C9orf1521
NM_0011102201
NM_0011102211
NM_006783GJB61
NM_0011102191
NM_012330MYST41
NM_003899ARHGEF71
NM_002595PCTK21
NM_205833IGSF11
NM_020987ANK31
NM_001149ANK31
NM_020724RNF1501
NM_006863LILRA11
NM_0011112831
NM_000618IGF11
NM_0011112841
NM_014636RALGPS11
NM_005819STX61
NM_004171SLC1A21
NM_001075098MOCS11
NM_005943MOCS11
NM_001037172ACPL21
NM_152282ACPL21
NM_001002274FCGR2B1
NM_001002275FCGR2B1
NM_001002273FCGR2B1
NM_004001FCGR2B1
NM_207582HERV-FRD1
NM_018717MAML31
NM_199169TMEPAI1
NM_199170TMEPAI1
NM_199171TMEPAI1
NM_020182TMEPAI1
NM_012306FAIM21
NM_003153STAT61
XM_377073LOC3764751
NM_052896CSMD21
XM_944835LOC6486911
NM_199418PRCP1
NM_005040PRCP1
NM_017623CNNM31
NM_199078CNNM31
NM_012199EIF2C11
NM_012294RAPGEF51
NM_012163LRRC291
NM_001004055LRRC291
NM_152479TTC9B1
NM_052840BRUNOL61
NM_207404ZNF6621
NM_002062GLP1R1
NM_207315LOC1296071
NM_001039577CCNL21
NM_016190CRNN1
XM_001124203LOC7302731
XM_944531LOC6459491
XM_933289LOC6459491
NM_198859PRICKLE21
NM_177549PRNT1
NM_003644GAS71
NM_201432GAS71
NM_201433GAS71
NM_018356C5orf221
NM_001795CDH51
NM_0010996661
NM_001001330REEP31
NM_001114ADCY71
NM_024895PDZD71
NM_025010KLHL181
NM_178275DKFZp434B12311
NM_001002265MARCH81
NM_145021MARCH81
NM_001002266MARCH81
NM_203374ZNF7841
NM_020205OTUD7B1
NM_001008407BCAP291
NM_144579SFXN51
NM_0150381
NM_139125MASP11
NM_181724TMEM1191
NM_138572TBN1
NM_145282LOC1533281
NM_0011016761
NM_014840NUAK11
NM_198490RAB431
NM_001039589DPH21
NM_001384DPH21
NM_020927KIAA15761
NM_001001692FLJ451391
NM_001659ARF31
NM_014873LPGAT11
XM_171855LOC2552751
XM_941352LOC2552751
XM_001133075LOC7292621
XM_001125950LOC7278071
XM_925903FAM22A1
NM_000391TPP11
XM_001134327LOC7323241
NM_0207091
NM_033020TRIM331
NM_015906TRIM331
NM_005248FGR1
NM_001042747FGR1
NM_001042729FGR1
NM_000576IL1B1
XM_932840FBF11
XM_946193FBF11
XM_371079FBF11
XM_942657FBF11
NM_007030TPPP1
NM_0010988141
NM_001080457LRRC4B1
XM_936789LRRC4B1
NM_153283HYAL11
NM_153285HYAL11
NM_033159HYAL11
NM_153282HYAL11
NM_153281HYAL11
NM_007312HYAL11
NM_052854CREB3L11
NM_002569FURIN1
NM_016819OGG11
NM_001012508SPRN1
NM_021913AXL1
NM_001699AXL1
NM_001001701LOC4011521
NM_0011026031
NM_198893ZNF1601
NM_033288ZNF1601
NM_007188ABCB81
NM_020133AGPAT41
NM_006779CDC42EP21
NM_004348RUNX21
NM_001015051RUNX21
NM_001024630RUNX21
NM_004265FADS21
NM_182705FAM101B1
XM_001126665FAM101B1
NM_003734AOC31
NM_020939CPNE51
NM_006539CACNG31
NM_014112TRPS11
NM_152891PRSS331
NM_000365TPI11
XM_001133949LOC7297081
XM_001133950LOC7297081
XM_001133946LOC7297081
XM_001133945LOC7297081
XM_001133947LOC7297081
XM_001134262LOC7321651
XM_001134264LOC7321651
XM_001134261LOC7321651
XM_001134259LOC7321651
XM_001134257LOC7321651
XM_001134263LOC7321651
NM_001040146CTF81
NM_001039690CTF81
NM_001040144CTF81
NM_006091CORO2B1
NM_004561OVOL11
XM_001129344OVOL11
NM_032827ATOH81
NM_001712CEACAM11
NM_001024912CEACAM11
NM_020808SIPA1L21
NM_002880RAF11
NM_145893A2BP11
NM_145892A2BP11
NM_145891A2BP11
NM_015063SLC8A21
NM_182697UBE2H1
NM_003344UBE2H1
NM_014832TBC1D41
NM_002278KRT321
NM_001939DRP21
NM_014732KIAA05131
NM_004200SYT71
XM_943686SYT71
NM_000354SERPINA71
NM_177965C8orf371
NM_014418TCL61
NM_012468TCL61
NM_006317BASP11
NM_001012729DUXA1
NM_018667SMPD31
NM_032298SYT31
NM_031287SF3B51
NM_000462UBE3A1
NM_130839UBE3A1
NM_130838UBE3A1
NM_201648GLYAT1
NM_014056HIGD1A1
NM_0010996681
NM_0010996691
NM_152371C1orf931
NM_017548CDV31
NM_145027KIF61
NM_019032ADAMTSL41
NM_002730PRKACA1
NM_207518PRKACA1
NM_016021UBE2J11
NM_014655SLC25A441
NM_015269ZCCHC111
NM_001009881ZCCHC111
NM_001009882ZCCHC111
NM_031455CCDC31
NM_003629PIK3R31
NM_001009812LBX21
NM_002197ACO11
NM_145256LRRC251
NM_012141INTS61
NM_001039937INTS61
NM_001655ARCN11
NM_004177STX31
NM_015659RSL1D11
NM_012484HMMR1
NM_012485HMMR1
XM_937358LOC6423841
XM_926149LOC6423841
NM_203434IER5L1
XM_001128616LOC4010211
NM_172370DAOA1
NM_021117CRY21
NM_020776KIAA13281
NM_020717SHROOM41
NM_172337OTX21
NM_021728OTX21
NM_003028SHB1
XM_001132933LOC7323521
XM_001130794LOC7296161
NM_022371TOR3A1
NM_020801ARRDC31
NM_001057TACR21
NM_014657KIAA04061
NM_024689CXorf361
NM_012398PIP5K1C1
XM_001134208LOC7299751
NM_005629SLC6A81
NM_178191ATPIF11
NM_033637BTRC1
NM_003939BTRC1
NM_139284LGI41
NM_0010976201
NM_024326FBXL151
NM_020904PLEKHA41
NM_201520SLC25A351
NM_004102FABP31
NM_001008710RBPMS1
NM_001008711RBPMS1
NM_006867RBPMS1
NM_018958C15orf21
NM_001009905B3GNTL11
NM_002945RPA11
NM_207411XKR51
NM_001361DHODH1
NM_0011013721
NM_172097CATSPER21
NM_024577SH3TC21
NM_198484ZNF6211
NM_0010984141
NM_014982PCNX1
NM_024511C4orf151
XM_939100MYO5B1
NM_001080467MYO5B1
NM_024843CYBRD11
NM_005171ATF11
NM_197941ADAMTS61
NM_033631LUZP11
NM_001083591CUGBP21
NM_001025077CUGBP21
NM_006561CUGBP21
NM_001025076CUGBP21
NM_007007CPSF61
NM_015483KBTBD21
NM_001031835PHKB1
NM_000293PHKB1
NM_001040152PEG101
NM_015068PEG101
NM_019625ABCB91
NM_203444ABCB91
NM_019624ABCB91
NM_005419STAT21
NM_018947CYCS1
NM_152694ZCCHC51
NM_003722TP73L1
NM_007203PALM2-AKAP21
NM_147150PALM2-AKAP21
NM_001004065AKAP21
NM_016002SCCPDH1
NM_145053UBQLNL1
NM_024329EFHD21
NM_152511DUSP181
NM_175056ZPLD11
NM_177550SLC13A51
NM_182666UBE2E11
NM_003341UBE2E11
NM_152455ZSCAN291
NM_004878PTGES1
XM_926521LOC6531371
XM_928267LOC6532701
XM_926348LOC6426331
XM_927800LOC6430691
NM_001040665STEAP21
NM_152999STEAP21
NM_014155ZBTB441
NM_001002862DERL31
NM_198440DERL31
NM_032317WBSCR181
NM_025235TNKS21
XM_001127632LOC7285191
NM_004536NAIP1
NM_022892NAIP1
NM_000337SGCD1
NM_016734PAX51
NM_182756SPDYA1
NM_017742ZCCHC21
NM_006379SEMA3C1
NM_138375CABLES11
XM_931376SETD51
XM_944260SETD51
XM_931478SETD51
XM_944280SETD51
XM_931417SETD51
XM_944276SETD51
XM_939712SETD51
NM_001080517SETD51
XM_944303SETD51
XM_931455SETD51
XM_931447SETD51
XM_944298SETD51
XM_944295SETD51
XM_931444SETD51
XM_943326LOC6477091
XM_931073LOC6423431
NM_001262CDKN2C1
NM_078626CDKN2C1
NM_017664ANKRD101
NM_003113SP1001
NM_004393DAG11
NM_020783SYT41
NM_016297PCYOX11
NM_173667FLJ375431
NM_032447FBN31
NM_138344C14orf1521
NM_004329BMPR1A1
NM_182832PLAC41
NM_006558KHDRBS31
NM_005856RAMP31
NM_018977NLGN31
NM_001018060AIFM31
NM_144704AIFM31
NM_0010997331
NM_001117ADCYAP11
NM_000808GABRA31
NM_012179FBXO71
NM_001033024FBXO71
NM_014665LRRC141
NM_015111N4BP31
NM_138814PNPLA51
NM_005840SPRY31
NM_015455CNOT61
XM_927029PPP1R3E1
XM_940069PPP1R3E1
NM_006995BTN2A21
NM_181531BTN2A21
NM_134445CD99L21
NM_134446CD99L21
NM_031462CD99L21
NM_004132HABP21
NM_153036FAM26D1
NM_005576LOXL11
XM_001133864LOXL11
NM_001040709SYPL21
NM_020792AADACL11
XM_001127233LOC7306231
XM_001127599LOC7280551
NM_007038ADAMTS51
NM_004273CHST31
NM_020405PLXDC11
NM_006373VAT11
NM_020663RHOJ1
NM_0010992251
NM_144609CCDC431
NM_001001685FLJ450791
NM_001004342TRIM671
NM_001737C91
NM_006661PDE10A1
XM_097977FAM59B1
XM_940578FAM59B1
NM_138415PHF21B1
NM_002557OVGP11
NM_032581FAM126A1
NM_001031804MAF1
NM_014772KIAA04271
NM_133367PAQR81
NM_001082CYP4F21
NM_000896CYP4F31
NM_000332ATXN11
NM_052811TRIM131
NM_213590TRIM131
NM_001007278TRIM131
NM_005798TRIM131
XM_001125970LOC7304651
XM_001128450LOC7282291
XM_928466LOC6454261
XM_001131298LOC7309901
XM_001128423LOC7282291
NM_002581PAPPA1
NM_019083CCDC761
NM_001001874TPD52L31
NM_002677PMP21
XM_001127052LOC7305681
XM_001130247LOC7286061
NM_0142471
NM_001008693CST91
NM_001043SLC6A21
NM_0010986241
NM_033290MID11
NM_000381MID11
NM_001003682TTMB1
NM_002519NPAT1
NM_001024688NBN1
NM_002485NBN1
NM_001076778FAM107A1
NM_007177FAM107A1
NM_0010843931
NM_152339SPATA2L1
NM_001500GMDS1
NM_017729EPS8L11
NM_133180EPS8L11
NM_017415KLHL31
NM_001497B4GALT11
NM_015104KIAA04041
NM_001032394GPR1261
NM_020455GPR1261
NM_198569GPR1261
NM_001032395GPR1261
NM_015184PLCL21
XM_001132281LOC7287161
NM_002585PBX11
NM_001006616TSPAN171
NM_012171TSPAN171
NM_130465TSPAN171
NM_001005505CACNA2D21
NM_006030CACNA2D21
NM_0204591
NM_198935SS18L11
XM_928464LOC1465171
NM_004907IER21
NM_012193FZD41
NM_020713ZNF512B1
XM_944900LOC6437701
XM_931792LOC6437701
NM_005767P2RY51
NM_014413EIF2AK11
NM_032013NDRG31
NM_022477NDRG31
NM_022166XYLT11
XM_001125986LOC2838241
NM_004287GOSR21
NM_006718PLAGL11
NM_002656PLAGL11
NM_001080951PLAGL11
NM_001080954PLAGL11
NM_001080953PLAGL11
NM_001080956PLAGL11
NM_001080955PLAGL11
NM_001080952PLAGL11
NM_019555ARHGEF31
NM_018945PDE7B1
NM_015141GPD1L1
NM_003748ALDH4A11
NM_000824GLRB1
NM_024969FAM130A21
NM_005028PIP5K2A1
NM_153442GPR261
NM_000428LTBP21
XM_001130382LOC7308551
NM_001039199C20orf1211
NM_024331C20orf1211
NM_001025108AFF31
NM_002285AFF31
XM_927706LOC6445911
NM_021095SLC5A61
NM_005328HAS21
NM_130830LRRC151
NM_001008495TMEM641
NM_080928ASB151
NM_017599VEZT1
NM_145733SEPT31
NM_019106SEPT31
NM_001080501MGC31961
NM_001008390CGGBP11
NM_003663CGGBP11
NM_032449CC2D1B1
NM_004293GDA1
NM_014062NOB11
NM_006345SLC30A91
NM_033666ITGB11
NM_003681PDXK1
NM_019117KLHL41
NM_138270ATRX1
NM_000489ATRX1
XM_001128623LOC7288491
NM_002565P2RY41
NM_183075CYP2U11
NM_001017916CYB5611
NM_001915CYB5611
NM_001017917CYB5611
NM_015020PHLPPL1
NM_001023587ABCC51
NM_033100PCDH211
NM_021143ZNF201
NM_025107MYCT11
NM_0011055431
NM_001083614EARS21
XM_001128799MAP3K21
NM_015864C6orf321
NM_018951HOXA101
NM_153715HOXA101
NM_024795TM4SF201
NM_015245ANKS1A1
NM_145244DDIT4L1
NM_014548TMOD21
NM_0151771
NM_005334HCFC11
NM_006534NCOA31
NM_181659NCOA31
NM_005722ACTR21
NM_001005386ACTR21
XM_938207LOC6491251
NM_139240C1orf1051
NM_000564IL5RA1
NM_175726IL5RA1
NM_002158FOXN21
NM_152874FAS1
NM_152876FAS1
NM_152877FAS1
NM_152872FAS1
NM_152873FAS1
NM_152875FAS1
NM_194285SPTY2D11
NM_019903ADD31
NM_016824ADD31
NM_001121ADD31
NM_000842GRM51

Immunostimulatory motif search results


UGUGU - Judge et al. Sequence-dependent stimulation of the mammalian innate immune response by synthetic siRNA. Nat Biotechnol. 2005 Apr;23(4):457-62. (PMID: 15778705)
No Immunostimulatory motif found (UGUGU).

GUCCUUCAA - Hornung et al. Sequence-specific potent induction of IFN-alpha by short interfering RNA in plasmacytoid dendritic cells through TLR7. Nat Med. 2005 Mar;11(3):263-70. Epub 2005 Feb 20. (PMID: 15723075)
No Immunostimulatory motif found (GUCCUUCAA).



Settings used for search

Scores were calculated using scoring scheme SS1
Using AVG scoring mode.

Hit scores

ParameterSpec. Score cut-offColourComment
Hit to same geneColourHits to the same accession or genes with 100 percent identity (i.e. likely transcript variants). It is advisable to always check the genes hit.
High scoring hit≥80ColourHits are very likely to cause an off-target effect
Medium scoring hit<80¸≥40ColourHits with a small chance of causing an off target effect.
Low scoring hitColourHits very unlikely to cause an off-target effect

The mismatches are colour coded based on the weighting of that position.

Cut-offColour
MatchColour
Wobble base pairs (G-U)Colour
Critical bases (none)Colour
0.6 bitsColour
0.4 bitsColour
0.2 bitsColour

About SpecificityServer

SpecificityServer is designed to help you identify potential non-specific matches to your siRNA. It incorporates the latest information about non-specific matches (sequence-specific only).

Designed by: Alistair Chalk, Quan Du, Zicai Liang and Erik L.L. Sonnhammer


For more details about the algorithm "siRNA specificity searching incorporating mismatch tolerance data" Bioinformatics (2008) 24 (10): 1316-1317