Predicted Off-targets for siRNA "gaaguacucucugacaaca" Using siRNA SpecificityServer

Specificity Server [Result page v1.0]


SpecificityServer - An siRNA design program for determining siRNA specificity

Automatic recommendation
Not OK - siRNA is not specific

9 hits were found for GAAGUACUCUCUGACAACA (4 guide strand hits and 5 sense strand hits.)

Hits to Guide strand

AccessionGeneStartEndDetails of mismatchesSpec.
Score
MismatchesContiguousJackson style
specificity
Hits similar
gene
HSP
Score
HSP %
Identity
HSP lengthGene Length
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19
NM_024560 FLJ21963 1324 1342 GcAGUACUCUCUGACAAgg80 3 15 ac false   <90%   3033
XM_001132204 LOC732019 727 745 uuuGaACUCUCUGACAACA15 4 14 bc false   <90%   1405
NM_053043 PRR8 166 184 uuuGaACUCUCUGACAACA15 4 14 bc false   <90%   4382
NM_002290 LAMA4 1873 1891 ugcGgACUCUCUGACAACA15 4 14 bc false   <90%   6297


Hits to Sense strand






AccessionGeneStartEndDetails of mismatchesSpec.
Score
MismatchesContiguousJackson style
specificity
Hits similar
gene
HSP
Score
HSP %
Identity
HSP lengthGene Length
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19
NM_004171 SLC1A2 8069 8087 UGUUGUCAGAGAagACUUC70 2 12   false   <90%   12021
NM_024772 ZMYM1 304 322 UGUUuUCAGAGAGUgCUUC23 2 9 c false   <90%   4172
XR_017794 LOC572558 429 447 UGUUGUCAaAGAGUACaau8 4 8 c false   <90%   609
NM_144997 FLCN 1431 1449 UGUUGcCAGAGAGUACaga5 4 10 c false   <90%   3717
NM_000944 PPP3CA 764 782 UGUUGaCAGAGgGUACUUC2 2 7   false   <90%   4424


3 prime UTR Seed matches

Hits to same gene : 0
Hits to other genes : 1009
View matched accessions

3 prime seed match details

AccessionGene nameHits to this gene
NM_004685MTMR63
NM_015565ZNF2943
NM_003787NOL43
NM_025009CEP1352
NM_014660PHF142
NM_181443BTBD32
NM_014962BTBD32
NM_002745MAPK12
NM_006731FCMD2
NM_001079802FCMD2
NM_015082FSTL42
NM_020768KCTD162
NM_0011008292
NM_019067GNL3L2
NM_024627C22orf292
NM_138972BACE12
NM_012104BACE12
NM_138973BACE12
NM_138971BACE12
NM_203446SYNJ12
NM_003895SYNJ12
NM_020742NLGN4X2
NM_181332NLGN4X2
NM_001183ATP6AP12
NM_001024647RAB3IP2
NM_022456RAB3IP2
NM_175624RAB3IP2
NM_175625RAB3IP2
NM_175623RAB3IP2
NM_018451CENPJ2
NM_021976RXRB2
NM_138423CASC42
NM_177974CASC42
NM_020738KIDINS2202
NM_001008495TMEM642
NM_012214MGAT4A2
NM_173631ZNF5472
NM_021629GNB42
NM_016376ANKFY12
NM_007249KLF122
NM_005158ABL22
NM_007314ABL22
NM_0011001082
NM_001080485ZNF2752
NM_001024843TNRC6B2
NM_015088TNRC6B2
NM_006729DIAPH22
NM_015284KIAA04672
NM_006581FUT92
NM_015226KIAA03502
NM_033035TSLP2
NM_138551TSLP2
NM_018084KIAA12122
NM_001083620GRIA22
NM_000826GRIA22
NM_001083619GRIA22
NM_001412EIF1AX2
NM_018662DISC12
NM_001012957DISC12
NM_001006623WDR332
NM_020854KIAA14682
NM_003643GCM12
NM_138348FAM105B2
NM_022080NAPB2
NM_018009TAPBPL1
NM_000031ALAD1
NM_001003945ALAD1
NM_001046SLC12A21
NM_153235TXLNB1
NM_003043SLC6A61
NM_000313PROS11
NM_016009SH3GLB11
NM_004921CLCA31
XM_939663KIAA15531
NM_001080450KIAA15531
NM_001455FOXO3A1
NM_201559FOXO3A1
NM_002943RORA1
NM_134262RORA1
NM_134261RORA1
NM_134260RORA1
NM_139286CDC261
NM_057175NARG11
NM_152316C11orf461
NM_032727INA1
NM_022763FNDC3B1
NM_201431RASSF61
NM_177532RASSF61
NM_194285SPTY2D11
NM_016025METTL91
NM_001077180METTL91
NM_001804CDX11
NM_002318LOXL21
NM_007332TRPA11
NM_173798ZCCHC121
NM_138703MAGEE21
NM_014309RBM91
NM_001082576RBM91
NM_001082577RBM91
NM_001031695RBM91
NM_001082578RBM91
NM_001082579RBM91
NM_002148HOXD101
NM_001918DBT1
NM_000108DLD1
NM_001003828PARVB1
NM_013327PARVB1
NM_021165FAM5B1
NM_018668VPS33B1
NM_012192FXC11
XM_171855LOC2552751
XM_941352LOC2552751
NM_018979WNK11
NM_006421ARFGEF11
NM_152388ALS2CR41
NM_001044385ALS2CR41
NM_000529MC2R1
NM_007159SLMAP1
NM_002040GABPA1
NM_014804KIAA07531
NM_145039MGC163851
XM_001124643LOC7304161
NM_001010862SPIN31
NM_016129COPS41
NM_0149811
NM_015040PIP5K31
NM_020662MRS2L1
NM_152275TTC30A1
NM_018018SLC38A41
NM_001024668LETMD11
NM_015416LETMD11
NM_003799RNMT1
NM_024818UBE1DC11
NM_198329UBE1DC11
NM_032432ABLIM21
NM_001025199CHI3L21
NM_001025197CHI3L21
NM_004000CHI3L21
NM_016040TMED51
NM_138731MIPOL11
NM_004932CDH61
NM_006290TNFAIP31
NM_030613ZFP21
NM_001609ACADSB1
NM_001776ENTPD11
NM_0010981751
NM_152529GPR1551
NM_001033045GPR1551
NM_207440FLJ264431
NM_000833GRIN2A1
XM_927767LOC6535051
XM_928338LOC6535981
XM_927706LOC6445911
XM_941684LOC2204331
XM_930211LOC2204331
XM_934711LOC6473131
XM_943547LOC6473131
NM_000637GSR1
NM_004797ADIPOQ1
NM_080632UPF3B1
NM_023010UPF3B1
XM_371691LOC3892031
NM_020474GALNT11
NM_173484KLF171
NM_001048226DBNDD21
NM_001048222DBNDD21
NM_001048224DBNDD21
NM_001025108AFF31
NM_002285AFF31
NM_021117CRY21
NM_006245PPP2R5D1
NM_180976PPP2R5D1
NM_180977PPP2R5D1
NM_138434C7orf291
NM_033083EAF11
NM_024873TNIP31
NM_020438DOLPP11
NM_032509RBM131
NM_001080975REPS21
NM_004726REPS21
XM_944366LOC3752951
XM_374020LOC3752951
NM_014612FAM120A1
NM_001033855DCLRE1C1
NM_001033857DCLRE1C1
NM_001033858DCLRE1C1
NM_022487DCLRE1C1
NM_006557DMRT21
NM_006912RIT11
NM_152630FAM46D1
NM_000554CRX1
NM_006330LYPLA11
NM_173463DKFZp761B1071
NM_007050PTPRT1
NM_133170PTPRT1
XM_940314NLF21
NM_001007595NLF21
NM_015326SRGAP21
NM_020463SMEK21
NM_007043KRR11
NM_021205RHOU1
NM_007345ZNF2361
NM_001031746C10orf721
NM_0333901
NM_014481APEX21
NM_030968C1QTNF11
NM_198594C1QTNF11
NM_198593C1QTNF11
NM_022459XPO41
NM_015090NFASC1
NM_054114TAGAP1
NM_152133TAGAP1
NM_023112OTUB21
NM_001018111PODXL1
NM_005397PODXL1
NM_020935USP371
NM_004155SERPINB91
NM_152734C6orf891
NM_024795TM4SF201
NM_152876FAS1
NM_152877FAS1
NM_152873FAS1
NM_152875FAS1
NM_198513PHF20L11
NM_182610SAMD71
NM_023080C8orf331
NM_001006114C21orf571
NM_002396ME21
NM_030634ZNF4361
NM_001077195ZNF4361
NM_015025MYT1L1
NM_004154P2RY61
NM_175932PSMD131
NM_002817PSMD131
NM_020755SERINC11
XM_941162LOC4009501
XM_376049LOC4009501
NM_020731AHRR1
NM_007271STK381
NM_016083CNR11
NM_033181CNR11
XM_934619LOC2845771
XM_945015LOC2845771
NM_012402ARFIP21
NM_0247761
NM_178172LOC3383281
NM_006373VAT11
NM_022127SLC28A31
NM_021269ZNF7081
NM_0010854011
NM_020162DHX331
NM_020894KIAA15301
NM_030667PTPRO1
NM_002848PTPRO1
NM_030668PTPRO1
NM_030669PTPRO1
NM_030671PTPRO1
NM_030670PTPRO1
NM_000348SRD5A21
NM_001759CCND21
NM_006951TAF51
NM_016531KLF31
NM_024770METTL81
NM_181877ZSCAN21
NM_138813ATP8B31
NM_001003791ERLIN21
NM_001003790ERLIN21
NM_001007224GIMAP61
NM_024711GIMAP61
NM_015455CNOT61
NM_020375C12orf51
NM_052882ZIM31
NM_015396ARMC81
NM_080819GPR781
NM_058246DNAJB61
NM_000791DHFR1
NM_176815DHFRL11
NM_004273CHST31
NM_032933C18orf451
NM_175876EXOC81
NM_001032290PSRC11
NM_001032291PSRC11
NM_032636PSRC11
NM_001005290PSRC11
NM_003943GENX-34141
NM_032701SUV420H21
NM_178468FAM83C1
XM_001127175LOC7283201
NM_000926PGR1
NM_0153061
NM_004628XPC1
NM_015529MOXD11
NM_020752GPR1581
NM_173598KSR21
NM_001343DAB21
NM_004119FLT31
NM_173808NEGR11
NM_002072GNAQ1
NM_0010999521
NM_002222ITPR11
NM_005630SLCO2A11
NM_015447CAMSAP11
NM_006359SLC9A61
NM_001042537SLC9A61
NM_023929ZBTB101
NM_0011055391
NM_018373SYNJ2BP1
NM_016231NLK1
NM_199246CCNG11
NM_004060CCNG11
NM_003729RTCD11
NM_005536IMPA11
NM_019014POLR1B1
NM_025160WDR261
NM_006154NEDD41
NM_198400NEDD41
NM_004170SLC1A11
NM_032521PARD6B1
NM_152420C9orf411
NM_033141MAP3K91
NM_207044ENSA1
NM_207043ENSA1
NM_207047ENSA1
NM_001039547GK51
NM_016206VGLL31
NM_005604POU3F21
NM_000639FASLG1
NM_198196CD961
NM_005816CD961
NM_000428LTBP21
NM_016651DACT11
NM_001079520DACT11
NM_022153C10orf541
NM_015920RPS27L1
NM_152376UBXD31
NM_014967MTMR151
NM_001012968LOC1398861
NM_005160ADRBK21
NM_015508TIPARP1
NM_176814ZNF8001
NM_016287HP1BP31
NM_199000LHFPL31
NM_181425FXN1
NM_000144FXN1
NM_153256C10orf471
NM_003025SH3GL11
NM_015059TLN21
NM_018999KIAA11281
NM_032458PHF61
NM_001015877PHF61
NM_004153ORC1L1
NM_024817THSD41
NM_018958C15orf21
NM_198281GPRIN31
XM_001130850LOC6458221
NM_006115PRAME1
NM_206954PRAME1
NM_206955PRAME1
NM_206953PRAME1
NM_206956PRAME1
NM_144578C14orf321
NM_001030055ARHGAP51
NM_001173ARHGAP51
NM_002207ITGA91
NM_002310LIFR1
NM_201525GPR561
NM_201524GPR561
NM_005682GPR561
NM_014817KIAA06441
NM_016413CPB21
NM_001872CPB21
NM_018126TMEM331
NM_018947CYCS1
NM_139076CCDC981
NM_005810KLRG11
NM_004473FOXE11
NM_014898ZFP301
NM_006111ACAA21
NM_005458GABBR21
NM_024577SH3TC21
NM_024545SAP1301
NM_030891LRRC31
NM_005858AKAP81
NM_152589C12orf501
NM_006691XLKD11
NM_020774MIB11
NM_001040157KIAA17121
XM_001129292MBOAT21
NM_032236USP481
NM_004277SLC25A271
NM_016059PPIL11
NM_002655PLAG11
NM_053042KIAA17291
NM_020403PCDH91
NM_203487PCDH91
NM_0011003971
NM_007023RAPGEF41
NM_198549FAM73A1
NM_031423NUF21
NM_145697NUF21
NM_002260KLRC21
NM_007328KLRC11
NM_213658KLRC11
NM_002259KLRC11
NM_213657KLRC11
NM_016052RRP151
NM_000963PTGS21
NM_033011PLAT1
NM_000930PLAT1
NM_138635H2AFV1
NM_018921PCDHGA91
NM_018923PCDHGB21
NM_018929PCDHGC51
NM_002588PCDHGC31
NM_018917PCDHGA41
NM_018918PCDHGA51
NM_032088PCDHGA81
NM_018915PCDHGA21
NM_018922PCDHGB11
NM_003736PCDHGB41
NM_018927PCDHGB71
NM_018924PCDHGB31
NM_018914PCDHGA111
NM_018920PCDHGA71
NM_018913PCDHGA101
NM_018916PCDHGA31
NM_018926PCDHGB61
NM_003735PCDHGA121
NM_032403PCDHGC31
NM_032092PCDHGA111
NM_018912PCDHGA11
NM_018919PCDHGA61
NM_018925PCDHGB51
NM_018928PCDHGC41
NM_0011031491
NM_018215FLJ107811
NM_004538NAP1L31
NM_001001931MTUS11
NM_001001924MTUS11
NM_020749MTUS11
NM_001001925MTUS11
NM_031431COG31
NM_016424CROP1
NM_022346NCAPG1
NM_004304ALK1
NM_173558FGD21
NM_138620DDX311
NM_001025073C3orf171
NM_015412C3orf171
NM_001025072C3orf171
NM_0121941
NM_006661PDE10A1
NM_000600IL61
NM_003710SPINT11
NM_001032367SPINT11
NM_181642SPINT11
NM_004822NTN11
NM_178554KY1
NM_054110GALNTL21
NM_199003THAP11
NM_004200SYT71
XM_943686SYT71
NM_0334031
NM_177996EPB41L11
NM_012156EPB41L11
NM_000252MTM11
NM_031420MRPL91
NM_018706DHTKD11
NM_031442TMEM471
NM_173473C10orf1041
NM_020845PITPNM21
NM_182606TMPRSS11A1
NM_014743KIAA02321
NM_0011005901
NM_002251KCNS11
NM_000430PAFAH1B11
NM_020390EIF5A21
NM_023008FLJ129491
NM_030965ST6GALNAC51
NM_006235POU2AF11
NM_139018CD300LF1
XM_001130012LOC7315471
XM_001129569LOC7291771
NM_031435THAP21
NM_004654USP9Y1
NM_017848FAM120C1
NM_004430EGR31
XM_001130047EGR31
NM_007361NID21
NM_031912SYT151
NM_022912REEP11
NM_015187KIAA07461
NM_001093ACACB1
NM_145321OSBPL31
NM_145320OSBPL31
NM_145322OSBPL31
NM_015550OSBPL31
NM_173551ANKS61
NM_024318LILRA61
NM_001259CDK61
NM_020335VANGL21
NM_001001685FLJ450791
NM_014231VAMP11
NM_199245VAMP11
NM_032052PATZ11
NM_030808NDEL11
NM_001025579NDEL11
NM_005472KCNE31
NM_002054GCG1
NM_001006655DKFZP564J1021
NM_015398DKFZP564J1021
XM_378454LOC2835471
XM_944809LOC2835471
NM_080797DIDO11
NM_013278IL17C1
NM_001007027ALG81
XM_001133420LOC7289911
NM_000242MBL21
NM_030806C1orf211
XM_927029PPP1R3E1
XM_940069PPP1R3E1
NM_001897CSPG41
NM_052899GPRIN11
NM_016575NT5DC31
NM_002031FRK1
NM_031476CRISPLD21
NM_006045ATP9A1
NM_173075APBB21
NM_001080417ZNF6291
XM_938127ZNF6291
NM_006654FRS21
NM_001042555FRS21
NM_001682ATP2B11
NM_001001323ATP2B11
NM_018650MARK11
NM_033091TRIM41
NM_033017TRIM41
NM_005828WDR681
NM_016472C14orf1291
NM_006803AP3M21
NM_007362NCBP21
NM_001042540NCBP21
NM_005590MRE11A1
NM_005591MRE11A1
NM_198578LRRK21
NM_012174FBXW81
NM_153348FBXW81
NM_138701C7orf111
NM_020405PLXDC11
NM_024077SECISBP21
NM_205846PRELID21
NM_138492PRELID21
NM_182960PRELID21
NM_031889ENAM1
NM_052937PCMTD11
NM_030664PTER1
NM_001001484PTER1
NM_001001549GRB101
NM_001001555GRB101
NM_001001550GRB101
NM_005311GRB101
XM_001133595LOC7293241
XM_001133813LOC7317571
NM_005127CLEC2B1
NM_1524191
NM_0209111
XM_001132119LOC2574071
XM_001129456LOC2574071
NM_003178SYN21
NM_020870SH3RF11
NM_014506TOR1B1
NM_004491GRLF11
NM_198074OR2C31
NM_012228MSRB21
NM_018124RFWD31
NM_181803UBE2C1
NM_004308ARHGAP11
NM_000909NPY1R1
NM_014893NLGN4Y1
NM_003302TRIP61
NM_006726LRBA1
NM_018054ARHGAP171
NM_001006634ARHGAP171
NM_014674EDEM11
NM_014106ZNF7701
NM_032866CGNL11
NM_020420DAZ41
NM_001005375DAZ41
NM_004081DAZ11
NM_001005786DAZ21
NM_020364DAZ31
NM_020363DAZ21
NM_001005785DAZ21
NM_019114EPB41L4B1
NM_052905FMNL21
NM_001040445ASB11
NM_020734FAM80B1
NM_153018ZFP31
NM_004992MECP21
NM_020705TBC1D241
NM_032932RAB11FIP41
NM_014803ZNF5181
NM_006504PTPRE1
NM_130435PTPRE1
NM_175910ZNF4931
NM_001076678ZNF4931
NM_001015508PURG1
NM_023037FRY1
NM_018439IMPACT1
NM_024557RIC31
NM_025010KLHL181
NM_052885SLC2A131
XM_001126893LOC7282181
NM_004521KIF5B1
NM_004383CSK1
NM_173536GABRG11
NM_006749SLC20A21
NM_004093EFNB21
NM_178507OAF1
XM_927451LOC6532261
NM_003133SRP91
NM_198441FLJ402961
XM_001132957LOC7292401
XM_001132952LOC7292331
XM_001132961LOC7292461
XM_001132965LOC7292501
XM_496115LOC4403381
XM_940659LOC4403381
NM_032740SFT2D31
NM_003918GYG21
NM_001079855GYG21
NM_0010992701
NM_0152761
NM_152793C7orf411
NM_000243MEFV1
NM_001419ELAVL11
NM_006867RBPMS1
NM_018059FLJ103241
NM_024410ODF11
NM_194255SLC19A11
NM_033428C9orf1231
NM_144949SOCS51
NM_014011SOCS51
NM_014904RAB11FIP21
NM_016297PCYOX11
NM_005668ST8SIA41
NM_004403DFNA51
NM_001012642GRAMD21
NM_012287CENTB21
NM_006931SLC2A31
NM_020918GPAM1
NM_006996SLC19A21
NM_014616ATP11B1
NM_030820COL21A11
NM_153619SEMA6D1
NM_153618SEMA6D1
NM_153616SEMA6D1
NM_153617SEMA6D1
NM_020858SEMA6D1
NM_022918TMEM1351
NM_023927GRAMD31
NM_0324531
NM_000666ACY11
NM_006038SPATA21
NM_005238ETS11
NM_001024809RARA1
NM_000964RARA1
NM_020546ADCY21
NM_003425ZNF451
NM_194247HNRPA31
NM_002416CXCL91
NM_018640LMO31
NM_001001395LMO31
NM_004487GOLGB11
NM_181787DPY19L41
NM_012158FBXL31
NM_002993CXCL61
NM_0010988441
NM_001005502CPM1
NM_198320CPM1
NM_001874CPM1
NM_020871LRCH21
NM_030647JHDM1D1
NM_003421ZNF37A1
NM_001007094ZNF37A1
NM_052950WDFY21
NM_016545IER51
XM_001127710LOC7286531
NM_183376ARRDC41
NM_002436MPP11
NM_133646ZAK1
NM_015071ARHGAP261
XM_928464LOC1465171
NM_019072SGTB1
NM_173588IGSF221
NM_0010988131
NM_0151461
NM_0010988121
NM_152680TMEM1541
NM_013309SLC30A41
NM_014333CADM11
NM_0010985171
NM_198887NUP431
NM_017654SAMD91
XM_001128311LOC7314491
XM_001129750LOC7289991
XM_001127122LOC4013971
XM_001132317LOC4013971
XM_001127063LOC4013971
NM_181458PAX31
NM_181460PAX31
NM_181457PAX31
NM_181459PAX31
NM_014021SSX2IP1
NM_152263TPM31
NM_024753TTC21B1
NM_001018058TFEC1
NM_012252TFEC1
NM_020125SLAMF81
NM_032927TMEM1281
NM_014999RAB211
NM_012238SIRT11
NM_014761KIAA01741
NM_000916OXTR1
NM_173562KCTD201
NM_033656BRWD11
NM_178505TMEM261
NM_020443NAV11
NM_002033FUT41
NM_013255MKLN11
NM_001083591CUGBP21
NM_001025077CUGBP21
NM_006561CUGBP21
NM_001025076CUGBP21
XM_938689KIAA16711
XM_371461KIAA16711
NM_001172ARG21
NM_001557IL8RB1
XM_001133543LOC6456871
XM_942897LOC6456871
XM_001133544LOC6456871
NM_002948RPL151
NM_001004053ACTBL11
NM_005028PIP5K2A1
NM_003362UNG1
NM_080911UNG1
NM_014686KIAA03551
NM_0209101
NM_001002811PDE4DIP1
NM_001002812PDE4DIP1
XM_001126165LOC7279271
NM_194298SLC16A91
NM_173050SCUBE11
NM_052932TMEM1231
NM_001033910TRAF51
NM_145759TRAF51
NM_004619TRAF51
NM_144726RNF1451
NM_001080541MGA1
XM_932727MGA1
XM_939296MGA1
XM_944092MGA1
NM_194434VAPA1
NM_003574VAPA1
NM_015277NEDD4L1
NM_0229061
NM_005180BMI11
NM_001080391SP1001
NM_198507TMEM1571
NM_014410CLUL11
NM_199167CLUL11
NM_052811TRIM131
NM_213590TRIM131
NM_001007278TRIM131
NM_005798TRIM131
NM_002202ISL11
XM_001125680LOC7304321
XM_001125705LOC7304321
NM_177422EIF2C31
NM_024852EIF2C31
NM_019054FAM35A1
NM_145165CHURC11
NM_005345HSPA1A1
NM_014826CDC42BPA1
NM_003607CDC42BPA1
NM_000692ALDH1B11
NM_021962ABR1
NM_001092ABR1
NM_203329CD591
NM_203331CD591
NM_203330CD591
NM_000611CD591
NM_030796ECOP1
XM_938287LOC6539301
XM_001129280LOC7290861
NM_020764CASKIN11
NM_147129ALS2CL1
NM_182775ALS2CL1
NM_015191SNF1LK21
NM_020717SHROOM41
XM_001128865LOC7284071
NM_203395IYD1
XM_943323LOC6467191
XM_933842LOC6467191
NM_001033RRM11
XM_001132868LOC7301941
XM_001133971LOC7319961
NM_001017405MAEA1
NM_005882MAEA1
XM_001129080LOC7307441
NM_173826C3orf231
NM_012409PRND1
NM_018712ELMOD11
NM_006505PVR1
NM_017761PNRC21
NM_001957EDNRA1
NM_017902HIF1AN1
NM_015027PDXDC11
XM_001125924KIAA02511
XM_001128013LOC7281931
XM_001126065LOC7304571
NM_001030001RPS291
NM_0010994131
NM_0010994121
NM_006766MYST31
NM_018279TMEM191
NM_000860HPGD1
NM_138467TYW31
NM_004540NCAM21
NM_0251851
NM_138727ST7L1
NM_138728ST7L1
NM_017744ST7L1
NM_0010853841
NM_032822FLJ146681
NM_138694PKHD11
NM_012248SEPHS21
NM_024800NEK111
NM_001044387ZNF5571
NM_024341ZNF5571
NM_001044388ZNF5571
XM_001130886FLJ370341
XM_001127207FLJ370341
NM_019012PLEKHA51
NM_014742TM9SF41
NM_182562FLJ397431
NM_018354C20orf461
NM_007175ERLIN21
NM_033505SELI1
NM_002711PPP1R3A1
NM_020193C11orf301
NM_013231FLRT21
NM_006080SEMA3A1
NM_199131VAX11
NM_020177FEM1C1
NM_0208461
NM_080628C20orf1181
NM_016279CDH91
NM_021632ZNF3501
NM_015291DNAJC161
NM_003214TEAD31
NM_016156MTMR21
NM_201278MTMR21
NM_201281MTMR21
NM_033426KIAA17371
NM_021777ADAM281
NM_152765C8orf461
NM_014071NCOA61
NM_001044722CSNK1G31
NM_001031812CSNK1G31
NM_001044723CSNK1G31
NM_004384CSNK1G31
NM_198081SCML41
NM_012388PLDN1
NM_001012969ADAL1
XM_936833LOC6441961
XM_927389LOC6441961
NM_020787ZNF6241
NM_014447ARFIP11
NM_001025595ARFIP11
NM_001025593ARFIP11
NM_002203ITGA21
NM_016422RNF1411
XM_037557KIAA09841
XM_939476KIAA09841
NM_000689ALDH1A11
NM_004774PPARBP1
NM_198273LYSMD31
NM_032286MED101
NM_012345NUFIP11
NM_024315C7orf231
NM_013286RBM15B1
NM_152903KBTBD61
NM_203364CAPRIN11
NM_022735ACBD31
NM_080491GAB21
NM_012296GAB21
NM_145312ZNF4851
NM_015911ZNF6911
NM_000538RFXAP1
NM_018490LGR41
NM_000809GABRA41
NM_025169ZNF1671
NM_007006NUDT211
NM_0071501
NM_014411NAG81
NM_006252PRKAA21
NM_005228EGFR1
NM_015691WWC31
NM_017791C14orf581
NM_004322BAD1
NM_032989BAD1
NM_017563IL17RD1
NM_001080973IL17RD1
NM_181493ITPA1
NM_033453ITPA1
NM_001004317LIN28B1
NM_018361AGPAT51
NM_017988SCYL21
NM_015269ZCCHC111
NM_001009881ZCCHC111
NM_001009882ZCCHC111
NM_001081492KRT801
NM_153456HS6ST31
NM_004661CDC231
NM_013352DSE1
NM_001080976DSE1
NM_014952BAHD11
NM_152606ZNF5401
NM_005813PRKD31
XM_927936LOC6448411
XM_935685LOC6419331
XM_938176LOC6448411
NM_001005210LRRC551
NM_022081HPS41
NM_152841HPS41
NM_003292TPR1
NM_018247TMEM30A1
NM_015879ST8SIA31
NM_004622TSN1
NM_173470TMEM321
NM_017706WDR551
NM_152726EFHA11
NM_016544RBJ1
NM_003932ST131
NM_001005609EDA1
NM_001399EDA1
NM_015332NUDCD31
XM_001128986LOC7289801
NM_173638NBPF151
NM_0011026631
NM_183372NBPF111
NM_004035ACOX11
NM_007292ACOX11
NM_006879MDM21
NM_006881MDM21
NM_002392MDM21
NM_006878MDM21
NM_006882MDM21
NM_152551C6orf1511
NM_033285TP53INP11
NM_001037954DIXDC11
NM_033425DIXDC11
NM_024757EHMT11
NM_1779662'-PDE1
NM_005966NAB11
NM_006837COPS51
NM_024501HOXD11
XM_939083TTC281
XM_929318TTC281
NM_004052BNIP31
NM_006865LILRA31
NM_201629TJP21
NM_004817TJP21
NM_005466MED61
NM_032105PPP1R12B1
NM_002481PPP1R12B1
NM_005822DSCR1L11
NM_021932RIC8A1
NM_170710WDR171
NM_181265WDR171
NM_020141C1orf1191
XM_001125727LOC7277781
XM_001125757LOC7277781
NM_014912CPEB31
NM_018834MATR31
NM_199189MATR31
NM_015576ERC21
NM_198468C6orf1671
NM_144563RPIA1
NM_001008492SEPT21
NM_006155SEPT21
NM_001008491SEPT21
NM_004404SEPT21
NM_032853MUM11
NM_004438EPHA41
NM_006626ZBTB61
NM_016630SPG211
NM_000910NPY2R1
NM_001080416MYBL11
XM_938064MYBL11
XM_933460MYBL11
NM_173559FLJ257911
NM_014251SLC25A131
NM_001017970TMEM30B1
NM_014989RIMS11
NM_198401ANKRD461
NM_003392WNT5A1
NM_001003937TSPYL61

Immunostimulatory motif search results


UGUGU - Judge et al. Sequence-dependent stimulation of the mammalian innate immune response by synthetic siRNA. Nat Biotechnol. 2005 Apr;23(4):457-62. (PMID: 15778705)
No Immunostimulatory motif found (UGUGU).

GUCCUUCAA - Hornung et al. Sequence-specific potent induction of IFN-alpha by short interfering RNA in plasmacytoid dendritic cells through TLR7. Nat Med. 2005 Mar;11(3):263-70. Epub 2005 Feb 20. (PMID: 15723075)
No Immunostimulatory motif found (GUCCUUCAA).



Settings used for search

Scores were calculated using scoring scheme SS1
Using AVG scoring mode.

Hit scores

ParameterSpec. Score cut-offColourComment
Hit to same geneColourHits to the same accession or genes with 100 percent identity (i.e. likely transcript variants). It is advisable to always check the genes hit.
High scoring hit≥80ColourHits are very likely to cause an off-target effect
Medium scoring hit<80¸≥40ColourHits with a small chance of causing an off target effect.
Low scoring hitColourHits very unlikely to cause an off-target effect

The mismatches are colour coded based on the weighting of that position.

Cut-offColour
MatchColour
Wobble base pairs (G-U)Colour
Critical bases (none)Colour
0.6 bitsColour
0.4 bitsColour
0.2 bitsColour

About SpecificityServer

SpecificityServer is designed to help you identify potential non-specific matches to your siRNA. It incorporates the latest information about non-specific matches (sequence-specific only).

Designed by: Alistair Chalk, Quan Du, Zicai Liang and Erik L.L. Sonnhammer


For more details about the algorithm "siRNA specificity searching incorporating mismatch tolerance data" Bioinformatics (2008) 24 (10): 1316-1317