Automatic recommendation
Warning - siRNA is specific for only the antisense (guide) strand
13 hits were found for GACAGUCAAUUGCAGACAA (7 guide strand hits and 6 sense strand hits.)
Hits to Guide strand
| Accession | Gene | Start | End | Details of mismatches | Spec. Score | Mismatches | Contiguous | Jackson style specificity | Hits similar gene | HSP Score | HSP % Identity | HSP length | Gene Length | ||||||||||||||||||
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | |||||||||||||
| NM_018212 | ENAH | 4830 | 4848 | a | c | a | A | G | U | C | A | A | U | U | G | C | A | a | A | C | A | A | 42 | 4 | 11 | c | false | <90% | 13109 | ||
| NM_001008493 | ENAH | 4893 | 4911 | a | c | a | A | G | U | C | A | A | U | U | G | C | A | a | A | C | A | A | 42 | 4 | 11 | c | false | <90% | 13172 | ||
| XM_001133195 | FLJ44450 | 1182 | 1200 | c | c | C | A | G | g | C | A | A | U | U | G | C | A | G | A | C | A | g | 18 | 4 | 12 | bc | false | <90% | 1599 | ||
| XM_001132353 | FLJ44450 | 1182 | 1200 | c | c | C | A | G | g | C | A | A | U | U | G | C | A | G | A | C | A | g | 18 | 4 | 12 | bc | false | <90% | 1599 | ||
| NM_021070 | LTBP3 | 1175 | 1193 | c | A | C | A | G | U | g | c | A | U | U | G | C | A | G | A | C | A | A | -30 | 3 | 11 | bc | false | <90% | 4064 | ||
| XM_045911 | STXBP5L | 2486 | 2504 | a | A | C | A | G | U | u | c | A | U | U | G | C | A | G | A | C | A | A | -30 | 3 | 11 | bc | false | <90% | 5179 | ||
| XM_938898 | STXBP5L | 2486 | 2504 | a | A | C | A | G | U | u | c | A | U | U | G | C | A | G | A | C | A | A | -30 | 3 | 11 | bc | false | <90% | 5179 | ||
Hits to Sense strand
| Accession | Gene | Start | End | Details of mismatches | Spec. Score | Mismatches | Contiguous | Jackson style specificity | Hits similar gene | HSP Score | HSP % Identity | HSP length | Gene Length | ||||||||||||||||||
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | |||||||||||||
| NM_002798 | PSMB6 | 590 | 608 | g | U | G | U | C | U | G | C | A | A | U | U | c | A | C | U | G | c | C | 84 | 3 | 11 | false | <90% | 849 | |||
| NM_001080444 | LOC150084 | 1787 | 1805 | a | a | a | U | C | U | G | C | A | A | U | U | G | A | C | U | G | U | C | 67 | 3 | 16 | bc | false | <90% | 2066 | ||
| XM_942239 | LOC150084 | 1787 | 1805 | a | a | a | U | C | U | G | C | A | A | U | U | G | A | C | U | G | U | C | 67 | 3 | 16 | bc | false | <90% | 2066 | ||
| NM_152504 | C20orf196 | 448 | 466 | c | U | G | U | C | U | G | C | A | A | g | U | G | c | C | U | G | U | C | 17 | 3 | 9 | c | false | <90% | 1203 | ||
| NM_001035518 | CEP250 | 1328 | 1346 | U | U | G | U | C | U | G | C | g | c | U | U | G | A | C | U | G | U | g | -47 | 3 | 8 | bc | false | <90% | 8197 | ||
| NM_007186 | CEP250 | 1328 | 1346 | U | U | G | U | C | U | G | C | g | c | U | U | G | A | C | U | G | U | g | -47 | 3 | 8 | bc | false | <90% | 8365 | ||
3 prime UTR Seed matches
Hits to same gene : 0
Hits to other genes : 1313
View matched accessions
3 prime seed match details
Immunostimulatory motif search results
UGUGU - Judge et al. Sequence-dependent stimulation of the mammalian innate immune response by synthetic siRNA. Nat Biotechnol. 2005 Apr;23(4):457-62. (PMID: 15778705)
No Immunostimulatory motif found (UGUGU).
GUCCUUCAA - Hornung et al. Sequence-specific potent induction of IFN-alpha by short interfering RNA in plasmacytoid dendritic cells through TLR7. Nat Med. 2005 Mar;11(3):263-70. Epub 2005 Feb 20. (PMID: 15723075)
No Immunostimulatory motif found (GUCCUUCAA).
Settings used for search
Scores were calculated using scoring scheme SS1
Using AVG scoring mode.
Hit scores
| Parameter | Spec. Score cut-off | Colour | Comment |
| Hit to same gene | Colour | Hits to the same accession or genes with 100 percent identity (i.e. likely transcript variants). It is advisable to always check the genes hit. | |
| High scoring hit | ≥80 | Colour | Hits are very likely to cause an off-target effect |
| Medium scoring hit | <80¸≥40 | Colour | Hits with a small chance of causing an off target effect. |
| Low scoring hit | Colour | Hits very unlikely to cause an off-target effect |
The mismatches are colour coded based on the weighting of that position.
| Cut-off | Colour |
| Match | Colour |
| Wobble base pairs (G-U) | Colour |
| Critical bases (none) | Colour |
| 0.6 bits | Colour |
| 0.4 bits | Colour |
| 0.2 bits | Colour |
SpecificityServer is designed to help you identify potential non-specific matches to your siRNA. It incorporates the latest information about non-specific matches (sequence-specific only).
Designed by: Alistair Chalk, Quan Du, Zicai Liang and Erik L.L. Sonnhammer