Automatic recommendation
Warning - siRNA is specific for only the antisense (guide) strand
29 hits were found for GGUAUAUGACUUUGCUUUU (7 guide strand hits and 22 sense strand hits.)
Hits to Guide strand
| Accession | Gene | Start | End | Details of mismatches | Spec. Score | Mismatches | Contiguous | Jackson style specificity | Hits similar gene | HSP Score | HSP % Identity | HSP length | Gene Length | ||||||||||||||||||
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | |||||||||||||
| NM_031217 | KIF18A | 3217 | 3235 | u | a | U | A | U | A | U | G | A | C | U | U | U | c | C | U | U | U | U | 67 | 3 | 11 | c | false | <90% | 3379 | ||
| NM_177969 | PPM1B | 1000 | 1018 | a | a | a | A | U | A | U | G | A | C | U | U | U | c | C | U | U | U | U | 47 | 4 | 10 | c | false | <90% | 1776 | ||
| NM_002706 | PPM1B | 1860 | 1878 | a | a | a | A | U | A | U | G | A | C | U | U | U | c | C | U | U | U | U | 47 | 4 | 10 | c | false | <90% | 2636 | ||
| NM_001033557 | PPM1B | 2280 | 2298 | a | a | a | A | U | A | U | G | A | C | U | U | U | c | C | U | U | U | U | 47 | 4 | 10 | c | false | <90% | 3056 | ||
| NM_002692 | POLE2 | 1403 | 1421 | G | G | c | A | U | A | U | G | A | C | U | a | U | G | C | U | U | U | g | 41 | 3 | 8 | false | <90% | 1690 | |||
| NM_032270 | LRRC8C | 1221 | 1239 | G | a | a | A | a | A | U | G | A | C | U | U | U | G | C | U | U | U | U | 17 | 3 | 14 | bc | false | <90% | 2575 | ||
| XM_001126406 | LOC727922 | 1628 | 1646 | G | G | U | A | a | A | U | a | A | C | U | U | U | G | C | U | U | U | g | -18 | 3 | 10 | bc | false | <90% | 8861 | ||
Hits to Sense strand
| Accession | Gene | Start | End | Details of mismatches | Spec. Score | Mismatches | Contiguous | Jackson style specificity | Hits similar gene | HSP Score | HSP % Identity | HSP length | Gene Length | ||||||||||||||||||
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | |||||||||||||
| NM_015346 | ZFYVE26 | 6967 | 6985 | c | A | A | A | G | C | A | A | A | G | U | C | A | U | A | U | A | C | a | 93 | 2 | 17 | abc | false | <90% | 9688 | ||
| NM_001040659 | TTC23 | 366 | 384 | g | A | A | A | G | C | A | A | A | G | U | C | c | U | A | U | u | C | C | 64 | 3 | 11 | false | <90% | 3245 | |||
| NM_001040658 | TTC23 | 488 | 506 | g | A | A | A | G | C | A | A | A | G | U | C | c | U | A | U | u | C | C | 64 | 3 | 11 | false | <90% | 3367 | |||
| NM_001040657 | TTC23 | 561 | 579 | g | A | A | A | G | C | A | A | A | G | U | C | c | U | A | U | u | C | C | 64 | 3 | 11 | false | <90% | 3440 | |||
| NM_001040656 | TTC23 | 612 | 630 | g | A | A | A | G | C | A | A | A | G | U | C | c | U | A | U | u | C | C | 64 | 3 | 11 | false | <90% | 3491 | |||
| NM_022905 | TTC23 | 683 | 701 | g | A | A | A | G | C | A | A | A | G | U | C | c | U | A | U | u | C | C | 64 | 3 | 11 | false | <90% | 3562 | |||
| NM_001040660 | TTC23 | 807 | 825 | g | A | A | A | G | C | A | A | A | G | U | C | c | U | A | U | u | C | C | 64 | 3 | 11 | false | <90% | 3686 | |||
| NM_001040655 | TTC23 | 929 | 947 | g | A | A | A | G | C | A | A | A | G | U | C | c | U | A | U | u | C | C | 64 | 3 | 11 | false | <90% | 3808 | |||
| NM_004379 | CREB1 | 2164 | 2182 | u | A | A | A | G | C | A | A | A | G | U | g | A | U | A | U | A | u | a | 56 | 4 | 10 | false | <90% | 2964 | |||
| NM_134442 | CREB1 | 2206 | 2224 | u | A | A | A | G | C | A | A | A | G | U | g | A | U | A | U | A | u | a | 56 | 4 | 10 | false | <90% | 3006 | |||
| XR_019366 | LOC401959 | 126 | 144 | A | A | A | A | G | C | A | A | A | G | U | C | A | U | g | U | g | g | u | 44 | 4 | 14 | c | false | <90% | 861 | ||
| XR_017431 | LOC401959 | 126 | 144 | A | A | A | A | G | C | A | A | A | G | U | C | A | U | g | U | g | g | u | 44 | 4 | 14 | c | false | <90% | 861 | ||
| XR_019130 | RPL12P6 | 39 | 57 | g | A | A | A | u | C | A | A | A | G | U | C | g | U | A | U | A | C | C | 36 | 3 | 7 | false | <90% | 564 | |||
| XR_016704 | RPL12P6 | 39 | 57 | g | A | A | A | u | C | A | A | A | G | U | C | g | U | A | U | A | C | C | 36 | 3 | 7 | false | <90% | 564 | |||
| NM_018349 | MCTP2 | 687 | 705 | A | A | A | A | G | u | A | A | A | G | U | C | A | U | A | U | A | u | a | 29 | 3 | 11 | c | false | <90% | 2637 | ||
| XR_017614 | LOC645688 | 1811 | 1829 | g | A | g | A | u | C | A | A | A | G | U | C | A | U | A | U | A | C | C | 26 | 3 | 14 | bc | false | <90% | 2338 | ||
| XR_019524 | LOC645688 | 1811 | 1829 | g | A | g | A | u | C | A | A | A | G | U | C | A | U | A | U | A | C | C | 26 | 3 | 14 | bc | false | <90% | 2338 | ||
| NM_021998 | ZNF711 | 2909 | 2927 | A | A | A | A | G | C | A | A | A | G | a | C | A | a | A | U | A | C | u | 14 | 3 | 10 | c | false | <90% | 4182 | ||
| NM_002853 | RAD1 | 1832 | 1850 | A | A | A | A | G | C | A | A | A | G | g | C | A | U | A | U | g | g | g | 7 | 4 | 10 | c | false | <90% | 4683 | ||
| NM_133377 | RAD1 | 1914 | 1932 | A | A | A | A | G | C | A | A | A | G | g | C | A | U | A | U | g | g | g | 7 | 4 | 10 | c | false | <90% | 4765 | ||
| NM_000922 | PDE3B | 3642 | 3660 | A | A | A | A | G | a | A | A | A | G | U | C | A | U | A | U | u | g | a | 5 | 4 | 10 | c | false | <90% | 4784 | ||
| NM_003795 | SNX3 | 1380 | 1398 | A | A | A | A | G | C | A | A | A | u | U | u | A | U | A | U | A | C | u | -21 | 3 | 9 | false | <90% | 1496 | |||
3 prime UTR Seed matches
Hits to same gene : 0
Hits to other genes : 1392
View matched accessions
3 prime seed match details
Immunostimulatory motif search results
UGUGU - Judge et al. Sequence-dependent stimulation of the mammalian innate immune response by synthetic siRNA. Nat Biotechnol. 2005 Apr;23(4):457-62. (PMID: 15778705)
No Immunostimulatory motif found (UGUGU).
GUCCUUCAA - Hornung et al. Sequence-specific potent induction of IFN-alpha by short interfering RNA in plasmacytoid dendritic cells through TLR7. Nat Med. 2005 Mar;11(3):263-70. Epub 2005 Feb 20. (PMID: 15723075)
No Immunostimulatory motif found (GUCCUUCAA).
Settings used for search
Scores were calculated using scoring scheme SS1
Using AVG scoring mode.
Hit scores
| Parameter | Spec. Score cut-off | Colour | Comment |
| Hit to same gene | Colour | Hits to the same accession or genes with 100 percent identity (i.e. likely transcript variants). It is advisable to always check the genes hit. | |
| High scoring hit | ≥80 | Colour | Hits are very likely to cause an off-target effect |
| Medium scoring hit | <80¸≥40 | Colour | Hits with a small chance of causing an off target effect. |
| Low scoring hit | Colour | Hits very unlikely to cause an off-target effect |
The mismatches are colour coded based on the weighting of that position.
| Cut-off | Colour |
| Match | Colour |
| Wobble base pairs (G-U) | Colour |
| Critical bases (none) | Colour |
| 0.6 bits | Colour |
| 0.4 bits | Colour |
| 0.2 bits | Colour |
SpecificityServer is designed to help you identify potential non-specific matches to your siRNA. It incorporates the latest information about non-specific matches (sequence-specific only).
Designed by: Alistair Chalk, Quan Du, Zicai Liang and Erik L.L. Sonnhammer