Automatic recommendation
OK - siRNA is specific for both sense and antisense (guide) strands
10 hits were found for CCUACAUUGCAUGAAUAUA (4 guide strand hits and 6 sense strand hits.)
Hits to Guide strand
| Accession | Gene | Start | End | Details of mismatches | Spec. Score | Mismatches | Contiguous | Jackson style specificity | Hits similar gene | HSP Score | HSP % Identity | HSP length | Gene Length | ||||||||||||||||||
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | |||||||||||||
| NM_005845 | ABCC4 | 5008 | 5026 | u | C | U | A | a | A | U | U | G | C | A | U | G | A | A | U | A | g | g | 38 | 4 | 12 | c | false | <90% | 5871 | ||
| NM_015967 | PTPN22 | 2881 | 2899 | u | C | U | A | C | A | U | U | G | C | c | a | G | A | A | U | A | U | A | 2 | 3 | 9 | false | <90% | 3615 | |||
| NM_031900 | AGXT2 | 432 | 450 | C | C | U | c | C | A | a | U | G | C | A | U | G | A | A | U | A | U | g | -8 | 3 | 11 | bc | false | <90% | 2165 | ||
| NM_031422 | CHST9 | 1975 | 1993 | u | C | U | A | a | A | a | U | G | C | A | U | G | A | A | U | A | U | A | -19 | 3 | 12 | bc | false | <90% | 2219 | ||
Hits to Sense strand
| Accession | Gene | Start | End | Details of mismatches | Spec. Score | Mismatches | Contiguous | Jackson style specificity | Hits similar gene | HSP Score | HSP % Identity | HSP length | Gene Length | ||||||||||||||||||
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | |||||||||||||
| XM_938440 | LOC400682 | 2937 | 2955 | U | A | U | A | U | U | C | A | U | G | C | A | A | U | a | U | A | u | G | 72 | 2 | 14 | c | false | <90% | 3499 | ||
| NM_173531 | ZNF100 | 3688 | 3706 | U | A | U | A | U | U | C | A | U | G | C | A | A | U | a | U | A | u | G | 72 | 2 | 14 | c | false | <90% | 5665 | ||
| NM_002535 | OAS2 | 2380 | 2398 | c | A | g | A | U | U | C | A | U | G | C | A | c | U | G | U | A | G | G | 68 | 3 | 9 | false | <90% | 3647 | |||
| NM_007347 | AP4E1 | 322 | 340 | U | A | U | A | U | U | C | A | U | G | C | A | A | U | c | a | A | G | u | 44 | 3 | 14 | c | false | <90% | 6682 | ||
| NR_003255 | TSIX | 33639 | 33657 | U | A | U | A | U | g | C | A | U | G | C | A | u | U | G | U | A | G | a | 21 | 3 | 6 | false | <90% | 36861 | |||
| NM_003390 | WEE1 | 2385 | 2403 | U | A | U | A | U | U | C | A | U | u | C | A | A | U | G | U | c | u | u | -13 | 4 | 9 | c | false | <90% | 4232 | ||
3 prime UTR Seed matches
Hits to same gene : 0
Hits to other genes : 1220
View matched accessions
3 prime seed match details
Immunostimulatory motif search results
UGUGU - Judge et al. Sequence-dependent stimulation of the mammalian innate immune response by synthetic siRNA. Nat Biotechnol. 2005 Apr;23(4):457-62. (PMID: 15778705)
No Immunostimulatory motif found (UGUGU).
GUCCUUCAA - Hornung et al. Sequence-specific potent induction of IFN-alpha by short interfering RNA in plasmacytoid dendritic cells through TLR7. Nat Med. 2005 Mar;11(3):263-70. Epub 2005 Feb 20. (PMID: 15723075)
No Immunostimulatory motif found (GUCCUUCAA).
Settings used for search
Scores were calculated using scoring scheme SS1
Using AVG scoring mode.
Hit scores
| Parameter | Spec. Score cut-off | Colour | Comment |
| Hit to same gene | Colour | Hits to the same accession or genes with 100 percent identity (i.e. likely transcript variants). It is advisable to always check the genes hit. | |
| High scoring hit | ≥80 | Colour | Hits are very likely to cause an off-target effect |
| Medium scoring hit | <80¸≥40 | Colour | Hits with a small chance of causing an off target effect. |
| Low scoring hit | Colour | Hits very unlikely to cause an off-target effect |
The mismatches are colour coded based on the weighting of that position.
| Cut-off | Colour |
| Match | Colour |
| Wobble base pairs (G-U) | Colour |
| Critical bases (none) | Colour |
| 0.6 bits | Colour |
| 0.4 bits | Colour |
| 0.2 bits | Colour |
SpecificityServer is designed to help you identify potential non-specific matches to your siRNA. It incorporates the latest information about non-specific matches (sequence-specific only).
Designed by: Alistair Chalk, Quan Du, Zicai Liang and Erik L.L. Sonnhammer