Predicted Off-targets for siRNA "ccagcgguccugacuagua" Using siRNA SpecificityServer

Specificity Server [Result page v1.0]


SpecificityServer - An siRNA design program for determining siRNA specificity

Automatic recommendation
OK - siRNA is specific for both sense and antisense (guide) strands

1 hit was found for CCAGCGGUCCUGACUAGUA (1 guide strand hits and 0 sense strand hits.)

Hits to Guide strand

AccessionGeneStartEndDetails of mismatchesSpec.
Score
MismatchesContiguousJackson style
specificity
Hits similar
gene
HSP
Score
HSP %
Identity
HSP lengthGene Length
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19
NM_002417 MKI67 11452 11470 uCAGCGGUaCUGACUuGUA11 3 7 c false   <90%   12684



3 prime UTR Seed matches

Hits to same gene : 0
Hits to other genes : 844
View matched accessions

3 prime seed match details

AccessionGene nameHits to this gene
NM_032268ZNRF13
NM_015226KIAA03503
NM_199454PRDM163
NM_022114PRDM163
NM_173832ZFP413
NM_002634PHB2
NM_007014WWP22
NM_199424WWP22
NM_001001549GRB102
NM_001001555GRB102
NM_001001550GRB102
NM_005311GRB102
NM_214677CLEC4M2
NM_214679CLEC4M2
NM_214678CLEC4M2
NM_130853PTPRS2
NM_002850PTPRS2
NM_130855PTPRS2
NM_130854PTPRS2
NM_022170EIF4H2
NM_031992EIF4H2
NM_001077418FLJ221672
NM_001077416FLJ221672
NM_001077419FLJ221672
NM_003313TSTA32
NM_033064ATCAY2
XM_001134027LOC7318892
XM_001134374LOC7301322
NM_001174ARHGAP62
NM_203425C17orf822
NM_001039709CBFA2T22
NM_001032999CBFA2T22
NM_005093CBFA2T22
NM_001013693LDLRAD22
NM_015193ARC2
NM_004220ZNF2132
NM_014190ADD12
NM_001119ADD12
NM_014189ADD12
NM_176801ADD12
NM_013435RAX2
NM_178580HM132
NM_001002860BTBD72
XM_943034LOC6430852
XM_931304LOC6430852
NM_033446FAM125B2
NM_017720STAP22
NM_001013841STAP22
NM_016196RBM192
XM_001134334RBM192
NM_024086METT10D2
NM_032737LMNB22
NM_005137DGCR22
NM_138499PWWP22
NM_0010986372
NM_014665LRRC142
NM_003486SLC7A52
NM_032242PLXNA12
NM_152914MGC338942
NM_012306FAIM22
NM_032831TMEM142B2
NM_014770CENTG12
XM_933796SLC45A42
NM_015197PACS22
NM_0011009132
NM_001702BAI12
NM_004758BZRAP12
NM_138422LOC1131792
NM_006549CAMKK22
NM_153499CAMKK22
NM_153500CAMKK22
NM_172216CAMKK22
NM_172226CAMKK22
NM_173551ANKS62
NM_0011004082
NM_001033046C17orf622
NM_0011004072
NM_002203ITGA22
NM_177405CECR12
NM_017424CECR12
NM_199003THAP11
NM_199040NUDT41
NM_019094NUDT41
NM_014212HOXC111
NM_016089ZNF5891
NM_181500CUTL11
NM_001913CUTL11
NM_020808SIPA1L21
NM_014555TRPM51
NM_0010994551
NM_018340FLJ111511
NM_004781VAMP31
NM_004474FOXD21
NM_012316KPNA61
XM_936438LOC6473671
XM_928321LOC6452771
XM_001132018FLJ401251
NM_001080401FLJ401251
NM_022463NXN1
NM_053005HCCA21
NM_014967MTMR151
NM_003017SFRS31
NM_002232KCNA31
NM_173827COX181
NM_023074ZNF6491
NM_152715TBCEL1
NM_023004RTN4R1
NM_001299CNN11
NM_020972ZFYVE281
NM_005505SCARB11
NM_138393REEP61
NM_004621TRPC61
NM_000734CD2471
NM_198053CD2471
NM_024527ABHD81
NM_002461MVD1
NM_176782C1orf1791
NM_005291GPR171
NM_020708SLC12A51
XM_001127055MGC870421
XM_001128503MGC870421
XM_001128032MGC870421
NM_002150HPD1
NM_004269CRSP81
NM_001010883FAM102B1
NM_005276GPD11
NM_006557DMRT21
NM_033481FBXO91
NM_033480FBXO91
NM_012347FBXO91
NM_005184CALM31
NM_015604WDR21A1
NM_181340WDR21A1
NM_181341WDR21A1
NM_022078GPATCH31
NM_0010853721
NM_015913TXNDC121
NM_033141MAP3K91
NM_022549FEZ11
NM_006943SOX121
NM_005883APC21
NM_001042371LOC2838711
NM_016621PHF21A1
NM_0011018021
NM_004420DUSP81
NM_000112SLC26A21
NM_004592SFRS81
NM_032740SFT2D31
NM_000444PHEX1
NM_032854CORO61
XM_001133002LOC7288041
NM_004171SLC1A21
NM_017436A4GALT1
NM_001001791C10orf551
NM_015447CAMSAP11
NM_017451BAIAP21
NM_022063C10orf841
NM_175709CBX71
NM_014848SV2B1
NM_014562OTX11
NM_015714G0S21
NM_018085IPO91
NM_018656SLC35E31
NM_019857CTPS21
NM_175859CTPS21
NM_001042423SLC16A31
NM_001042422SLC16A31
NM_004207SLC16A31
NM_018364RSBN11
NM_0011013761
NM_014798PLEKHM11
XM_001128220PLEKHM11
XM_935188LOC4404561
NM_012448STAT5B1
NM_031448C19orf121
NM_001031726C19orf121
NM_052896CSMD21
NM_000113TOR1A1
NM_014035SNX241
NM_015289VPS391
NM_018058CRTAC11
NM_002034FUT51
NM_0011127411
NM_001007237IGSF31
NM_001542IGSF31
NM_001001795MGC708571
XM_001130238LOC7282831
XM_001132687LOC7310871
NM_014577BRD11
NM_033315RASL10B1
NM_032482DOT1L1
NM_024322CENPO1
NM_000336SCNN1B1
NM_152699SENP51
NM_001005361DNM21
NM_004945DNM21
NM_001005362DNM21
NM_001005360DNM21
NM_052847GNG71
NM_1829691
NM_130459TOR2A1
NM_182507KRT801
NM_001081492KRT801
NM_181521CMTM41
NM_0010988191
NM_0010988181
NM_000923PDE4C1
NM_006393NEBL1
NM_003458BSN1
NM_006093PRG31
NM_015168C19orf71
NM_177999ASB61
NM_017873ASB61
NM_019845RPRM1
NM_015025MYT1L1
NM_031231APBA2BP1
NM_031232APBA2BP1
NM_020526EPHA81
NM_019601SUSD21
NM_001918DBT1
NM_152468TMC81
NM_001004720NCK21
NM_001004722NCK21
NM_003581NCK21
NM_005860FSTL31
NM_145175FAM84A1
NM_203327SLC23A21
NM_005116SLC23A21
NM_001287CLCN71
NM_012111AHSA11
NM_016032ZDHHC91
NM_001008222ZDHHC91
NM_006052DSCR31
NM_001024380TM2D21
NM_078473TM2D21
NM_001024381TM2D21
NM_031940TM2D21
NM_000353TAT1
NM_013345GPR1321
NM_032296FLYWCH11
NM_000303PMM21
NM_024911GPR1771
XM_939744LOC6449611
XM_930420LOC6449611
NM_016488PPHLN11
NM_021949ATP2B31
NM_001001344ATP2B31
NM_016505ZCCHC171
NM_0011096621
NM_032017STK401
NM_146388MRPL41
NM_032595PPP1R9B1
NM_003221TFAP2B1
XM_001133948LOC7318551
NM_0010992941
XM_936510KIAA16441
XM_927238LOC6439881
XM_936418LOC6473471
NM_175609ARFGAP11
NM_018209ARFGAP11
XM_941762SETD1B1
XM_037523SETD1B1
NM_002147HOXB51
NM_199340LRRC37A31
NM_207117C14orf681
NM_014847UBAP2L1
NM_152757FLJ303131
NM_207307C3orf251
NM_198573C9orf1651
NM_017955CDCA41
NM_002978SCNN1D1
NM_001077263TMPRSS131
XM_943471LOC3888491
XM_373945LOC3888491
XM_379840LOC4025091
XM_935781LOC4025091
NM_017488ADD21
NM_001024401SBK11
NM_004104FASN1
NM_178830C19orf471
NM_003028SHB1
NM_145253FAM100A1
NM_001040661SLC29A41
NM_153247SLC29A41
NM_015229KIAA06641
NM_001909CTSD1
NM_176795HRAS1
NM_001626AKT21
NM_206967C16orf741
NM_020845PITPNM21
NM_007030TPPP1
NM_002473MYH91
NM_000068CACNA1A1
NM_145260OSR11
NM_001407CELSR31
NM_080797DIDO11
NM_002070GNAI21
NM_005258GCHFR1
XM_933614LOC6464341
NM_003119SPG71
NM_001045478LOC6449971
NM_139056ADAMTS161
NM_018013SOBP1
NM_020145SH3GLB21
XM_927936LOC6448411
XM_935685LOC6419331
XM_938176LOC6448411
NM_006927ST3GAL21
NM_016426GTSE11
NM_002653PITX11
NM_020429SMURF11
NM_181349SMURF11
NM_022450RHBDF11
NM_175078KRT771
NM_057160ARTN1
NM_003976ARTN1
NM_057091ARTN1
NM_057090ARTN1
NM_025164KIAA09991
NM_194255SLC19A11
NM_006562LBX11
NM_002608PDGFB1
NM_033016PDGFB1
NM_017902HIF1AN1
NM_000486AQP21
NM_144998STRA131
NM_002344LTK1
NM_206961LTK1
NM_004277SLC25A271
NM_001001349NKIRAS21
NM_017595NKIRAS21
NM_004488GP51
NM_023007JMJD41
NM_018218USP401
NM_000258MYL31
NM_032108SEMA6B1
NM_032588TRIM631
NM_016538SIRT71
NM_020133AGPAT41
NM_003003SEC14L11
NM_001037165FOXK11
NM_001077620PRCD1
NM_002080GOT21
NM_145045MGC209831
NM_004561OVOL11
XM_001129344OVOL11
NM_006898HOXD31
NM_001082575HRNBP31
NM_001018050POLR3H1
NM_138338POLR3H1
NM_001018052POLR3H1
XM_001126137LOC2849521
XM_001129340LOC2849521
NM_0010988111
NM_003648DGKD1
NM_152879DGKD1
NM_173588IGSF221
NM_152412ZNF5721
NM_003873NRP11
NM_152744SDK11
NM_006295VARS1
NM_005338HIP11
NM_004643PABPN11
NM_138736GNAO11
NM_198285WDR861
NM_194460RNF1261
XM_001128169LOC7286871
NM_024328THTPA1
NM_0011003901
NM_152558IQCE1
NM_017592IXL1
NM_001273CHD41
NM_144605SEPT121
NM_015553PIP3-E1
NM_213613SLC26A11
NM_022042SLC26A11
NM_015288PHF151
XM_001130886FLJ370341
XM_001127207FLJ370341
NM_207121FAM110A1
NM_001042353FAM110A1
NM_031424FAM110A1
NM_015166MLC11
NM_139202MLC11
NM_001003674C18orf11
NM_181483C18orf11
NM_181481C18orf11
NM_001003675C18orf11
NM_004338C18orf11
NM_181482C18orf11
NM_017438SETD41
NM_153259MCOLN21
NM_020064BARHL11
NM_000376VDR1
NM_001017535VDR1
NM_012264C22orf51
NM_002972SBF11
NM_001030005CPLX31
NM_001040260DCLK21
NM_001040261DCLK21
NM_001014283DCUN1D21
NM_005523HOXA111
NM_005336HDLBP1
NM_203346HDLBP1
NM_030626LRRC271
NM_004943DMWD1
NM_018941CLN81
XM_940825DLGAP31
NM_001080418DLGAP31
NM_052875VPS26B1
NM_000500CYP21A21
NM_032348MXRA81
NM_001150ANPEP1
NM_002936RNASEH11
NM_138969RDHE21
NM_002223ITPR21
NM_001008392CTDSPL1
NM_005808CTDSPL1
NM_001986ETV41
NM_001079675ETV41
XM_498491LOC4399941
XM_930748LOC6423611
NM_020310MNT1
NM_004798KIF3B1
NM_020804PACSIN11
NM_003681PDXK1
NM_014235UBL4A1
NM_030758OSBP21
NM_001692ATP6V1B11
NM_0011004271
NM_021159RAP1GDS11
NM_0011004281
NM_0011004291
NM_0011004261
NM_0011004301
NM_012164FBXW21
NM_174887IFT201
NM_178229IQGAP31
NM_004861GAL3ST11
NM_003077SMARCD21
NM_0010984261
NM_024979MCF2L1
NM_0011127321
NM_138983OLIG11
XM_929878LOC6469151
NM_052950WDFY21
NM_001106ACVR2B1
NM_017891C1orf1591
NM_138814PNPLA51
XM_499022LOC4411241
NM_033129SCRT21
NM_001838CCR71
NM_001528HGFAC1
NM_019595ITSN21
NM_006277ITSN21
NM_001024858SPTB1
NM_198856FLJ235881
NM_022785FLJ235881
NM_015015JMJD2B1
NM_0010996821
NM_024859MAGIX1
NM_0010996801
NM_0010996811
NM_001736C5AR11
XM_938374LOC6440101
NM_019062RNF1861
NM_133443GPT21
NM_019850NGEF1
NM_031215CABLES21
NM_023067FOXL21
NM_014931SAPS11
NM_004040RHOB1
NM_001032363C1orf1511
XM_931359LOC3387581
XM_944677LOC3387581
NM_000719CACNA1C1
NM_001010898SLC6A171
NM_181505PPP1R1B1
NM_032192PPP1R1B1
NM_052924RHPN11
NM_033420C19orf61
NM_006067COX4NB1
NM_003660PPFIA31
NM_014773KIAA01411
NM_001989EVX11
NM_021964ZNF1481
NM_000125ESR11
NM_000539RHO1
NM_001002265MARCH81
NM_145021MARCH81
NM_001002266MARCH81
NM_000738CHRM11
NM_174937TCERG1L1
NM_0010976201
XM_377073LOC3764751
NM_004463FGD11
NM_152274FAM58A1
XM_931376SETD51
XM_944260SETD51
XM_931478SETD51
XM_944280SETD51
XM_931417SETD51
XM_944276SETD51
XM_939712SETD51
NM_001080517SETD51
NM_005366MAGEA111
NM_001011544MAGEA111
NM_032355MON1A1
NM_001040439MAPK8IP31
NM_015133MAPK8IP31
NM_173561UNC5CL1
NM_012163LRRC291
NM_001004055LRRC291
NM_053056CCND11
NM_020807ZNF3191
NM_014359OPTC1
NM_0153061
NM_019009TOLLIP1
NM_002862PYGB1
NM_016578RSF11
NM_004455EXTL11
NM_005402RALA1
NM_181711GRASP1
NM_003216TEF1
NM_0010975771
NM_001145ANG1
NM_004204PIGQ1
NM_006668CYP46A11
NM_014718CLSTN31
XM_944850SLC45A41
NM_001080431SLC45A41
XM_942210LOC6505621
XM_173132LOC2556201
XM_001133189INPP5A1
NM_005539INPP5A1
NM_032137C3orf201
NM_003139SRPR1
NM_175922MGC353081
XM_039733KIAA09531
XM_940464KIAA09531
NM_152889CHST131
NM_018719CDCA7L1
NM_012292HMHA11
NM_033387FAM78A1
NM_001190BCAT21
NM_014351SULT4A11
NM_006336ZER11
NM_015694ZNF7771
NM_007073BVES1
NM_147147BVES1
NM_022049GPR881
NM_022082C20orf591
NM_003284TNP11
NM_207368LOC3482621
XM_001129857PDDC11
NM_182612PDDC11
NM_199054MKNK21
NM_198531ATP9B1
NM_001001561GGA11
NM_004383CSK1
NM_007032TRIOBP1
NM_001039141TRIOBP1
NM_018198DNAJC111
NM_033229TRIM151
NM_004519KCNQ31
NM_020211RGMA1
XM_937753PRDM61
XM_927647PRDM61
NM_004588SCN2B1
NM_014646LPIN21
NM_024653PRKRIP11
NM_003636KCNAB21
NM_172130KCNAB21
NM_001080484KIAA17511
XM_936307KIAA17511
NM_025227BPIL11
NM_025108C16orf591
XM_934676LOC6449251
XM_942888LOC6449251
XM_001130790KIAA18811
NM_001080400KIAA18811
NM_006788RALBP11
XM_001126103LOC7281881
NM_001029891PGAM41
NM_014157CCDC1131
NM_1735951
NM_015154MESDC21
NM_004748CCPG11
NM_020739CCPG11
NM_172367TUSC51
NM_001031672LOC6064951
NM_0010853821
NM_000219KCNE11
NM_020439CAMK1G1
NM_025106SPSB11
NM_002248KCNN11
NM_016049C14orf1221
NM_032885ATG4D1
NM_052998ADC1
NM_016035COQ41
NM_178172LOC3383281
NM_024757EHMT11
NM_004603STX1A1
NM_033397KIAA17541
NM_005253FOSL21
NM_014326DAPK21
NM_018921PCDHGA91
NM_018923PCDHGB21
NM_018929PCDHGC51
NM_002588PCDHGC31
NM_018917PCDHGA41
NM_018918PCDHGA51
NM_032088PCDHGA81
NM_018915PCDHGA21
NM_018922PCDHGB11
NM_003736PCDHGB41
NM_018927PCDHGB71
NM_018924PCDHGB31
NM_018914PCDHGA111
NM_018920PCDHGA71
NM_018913PCDHGA101
NM_018916PCDHGA31
NM_018926PCDHGB61
NM_003735PCDHGA121
NM_032403PCDHGC31
NM_032092PCDHGA111
NM_018912PCDHGA11
NM_018919PCDHGA61
NM_018925PCDHGB51
NM_018928PCDHGC41
NM_021020LZTS11
NM_018043TMEM16A1
NM_181491SURF51
NM_1524191
NM_000787DBH1
NM_001042574CRTC31
NM_022769CRTC31
NM_178310SNAI31
NM_017659QPCTL1
NM_003224ARFRP11
NM_003043SLC6A61
NM_006675TSPAN91
NM_007229PACSIN21
XM_936831IQSEC31
NM_015242CENTD21
NM_139181CENTD21
NM_001040118CENTD21
NM_130844WWOX1
NM_138479C1orf2131
NM_014909VASH11
NM_006371CRTAP1
NM_0011031491
NM_018215FLJ107811
NM_014772KIAA04271
NM_020203MEPE1
NM_020336KIAA12191
NM_016379VCX3A1
NM_013452VCX1
NM_016378VCX21
XM_001127115VCX3A1
NM_001001888VCX-C1
XM_001127089VCX3A1
NM_001001685FLJ450791
NM_052906LRRC621
NM_014586HUNK1
XM_001130956ANKS61
NM_021601CD79A1
NM_001783CD79A1
NM_007367RALY1
NM_016732RALY1
NM_207417FLJ460821
NM_015061JMJD2C1
NM_012121CDC42EP41
XM_037557KIAA09841
XM_939476KIAA09841
NM_052909KIAA19091
NM_001018070CORO1B1
NM_020441CORO1B1
XM_001131399LOC7319621
XM_001131436LOC7298221
NM_014952BAHD11
NM_015267CUTL21
NM_022745ATPAF11
NM_001042546ATPAF11
XM_940069PPP1R3E1
XM_927029PPP1R3E1
NM_001083314CHMP1A1
NM_002768CHMP1A1
NM_198689KRTAP10-71
NM_0011097601
NM_025161C17orf701
NM_023936MRPS341
NM_052926PNMA51
NM_0011031511
NM_0011031501
NM_001094ACCN11
NM_183377ACCN11
NM_005550KIFC31
XM_001127262LOC7306671
XM_001133979LOC7297401
NM_001003398BICD11
NM_001714BICD11
NM_006361HOXB131
NM_138387G6PC31
NM_001040214TCBA11
NM_002035FVT11
XM_001133556LOC7291351
XM_001133211LOC7290031
NM_007360KLRK11
NM_022467CHST81
NM_144599NIPA11
NM_030806C1orf211
XM_001130794LOC7296161
XM_001132933LOC7323521
NM_018045BSDC11
NM_003200TCF31
NM_032780TMEM251
NM_199262SP61
NM_004976KCNC11
NM_015321CRTC11
NM_0010984821
NM_001005210LRRC551
NM_198935SS18L11
NM_006743RBM31
NM_002657PLAGL21
NM_177457LYNX11
NM_177477LYNX11
NM_177476LYNX11
NM_194452RNF1211
NM_194453RNF1211
NM_018320RNF1211
NM_152643KNDC11
NM_004430EGR31
XM_001130047EGR31
XM_941692LOC3878901
XM_370707LOC3878901
NM_004917KLK41
NM_001031801LIMK21
NM_004265FADS21
NM_014935PLEKHA61
NM_005803FLOT11
NM_015299KIAA03231
NM_005994TBX21
NM_017432PTOV11
NM_014138TMEM291
NM_0010996841
XM_001126068LOC7278661
XM_001126053LOC7278661
XM_001126035LOC7278661
XM_001126077LOC7278661
NM_005158ABL21
NM_007314ABL21
NM_0011001081
NM_004796NRXN31
NM_0011052501
NM_138970NRXN31
NM_015471NSL11
NM_001042549NSL11
NM_000693ALDH1A31
NM_001420ELAVL31
NM_032281ELAVL31
NM_024654NOL91
NM_001271CHD21
NM_020803KLHL81
NM_178564NRBP21
NM_022048CSNK1G11
NM_001039966GPR301
NM_001505GPR301
NM_0010982011
NM_020531C20orf31
NM_007374SIX61
NM_145013C11orf451
NM_145798OSBPL71
NM_173552C3orf581
NM_000913OPRL11
NM_182647OPRL11
NM_002235KCNA61
NM_178448C9orf1401
NM_006387CHERP1
NM_014286FREQ1
NM_138412RDH131
NM_198087ZNF2001
NM_198088ZNF2001
NM_003454ZNF2001
NM_078483SLC36A11
NM_024656GLT25D11
NM_007051FAF11
NM_004637RAB7A1
NM_001003682TTMB1
NM_001039355SLC25A291
NM_014694ADAMTSL21
NM_022762RMND5B1
XM_926522LOC6423501
NM_020400GPR921
NM_000431MVK1
NM_002957RXRA1
NM_001033518WIPI21
NM_001033519WIPI21
NM_015610WIPI21
NM_001033520WIPI21
NM_016003WIPI21
NM_139321ATRN1
NM_001001716NFKBIB1
NM_001007229SPOP1
NM_001007230SPOP1
NM_001007226SPOP1
NM_003563SPOP1
NM_001007228SPOP1
NM_001007227SPOP1
NM_001001694FLJ419931
NM_145652WFDC51
NM_005370RAB8A1
NM_014659HISPPD2A1
NM_001024463HISPPD2A1
NM_003629PIK3R31
NM_004339PTTG1IP1
XM_939981LOC6469761
XM_929947LOC6469761
NM_001014342FLG21
NM_198256E2F61
NM_199367SPG71
NM_001029859KCTD211
NM_004054C3AR11
NM_138621BCL2L111
NM_006538BCL2L111
NM_000104CYP1B11
NM_004886APBA31
NM_001008492SEPT21
NM_006155SEPT21
NM_001008491SEPT21
NM_004404SEPT21
NM_020820PREX11
NM_022780RMND5A1
NM_021570BARX11
NM_033025SYDE11
NM_014480ZNF5441
NM_015174KIAA10861
NM_003108SOX111
NM_022073EGLN31
NM_002460IRF41
NM_144664FAM76B1
NM_018246CCDC251
NM_001003716RECQL51
NM_152444ZADH11

Immunostimulatory motif search results


UGUGU - Judge et al. Sequence-dependent stimulation of the mammalian innate immune response by synthetic siRNA. Nat Biotechnol. 2005 Apr;23(4):457-62. (PMID: 15778705)
No Immunostimulatory motif found (UGUGU).

GUCCUUCAA - Hornung et al. Sequence-specific potent induction of IFN-alpha by short interfering RNA in plasmacytoid dendritic cells through TLR7. Nat Med. 2005 Mar;11(3):263-70. Epub 2005 Feb 20. (PMID: 15723075)
No Immunostimulatory motif found (GUCCUUCAA).



Settings used for search

Scores were calculated using scoring scheme SS1
Using AVG scoring mode.

Hit scores

ParameterSpec. Score cut-offColourComment
Hit to same geneColourHits to the same accession or genes with 100 percent identity (i.e. likely transcript variants). It is advisable to always check the genes hit.
High scoring hit≥80ColourHits are very likely to cause an off-target effect
Medium scoring hit<80¸≥40ColourHits with a small chance of causing an off target effect.
Low scoring hitColourHits very unlikely to cause an off-target effect

The mismatches are colour coded based on the weighting of that position.

Cut-offColour
MatchColour
Wobble base pairs (G-U)Colour
Critical bases (none)Colour
0.6 bitsColour
0.4 bitsColour
0.2 bitsColour

About SpecificityServer

SpecificityServer is designed to help you identify potential non-specific matches to your siRNA. It incorporates the latest information about non-specific matches (sequence-specific only).

Designed by: Alistair Chalk, Quan Du, Zicai Liang and Erik L.L. Sonnhammer


For more details about the algorithm "siRNA specificity searching incorporating mismatch tolerance data" Bioinformatics (2008) 24 (10): 1316-1317