Automatic recommendation
OK - siRNA is specific for both sense and antisense (guide) strands
21 hits were found for GGCCAUCGAUGCUGGAUUU (14 guide strand hits and 7 sense strand hits.)
Hits to Guide strand
Accession | Gene | Start | End | Details of mismatches | Spec. Score | Mismatches | Contiguous | Jackson style specificity | Hits similar gene | HSP Score | HSP % Identity | HSP length | Gene Length | ||||||||||||||||||
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | |||||||||||||
NM_007163 | SLC14A2 | 2727 | 2745 | G | G | C | C | A | U | C | G | c | U | G | C | a | G | G | A | U | U | U | 29 | 2 | 8 | false | <90% | 4166 | |||
NM_001034842 | PTCHD3 | 272 | 290 | G | G | C | C | g | U | C | G | A | U | G | C | U | G | G | A | c | g | a | 17 | 4 | 11 | c | false | <90% | 2369 | ||
NM_005135 | SLC12A6 | 1057 | 1075 | G | G | C | C | A | U | C | u | A | U | G | C | U | G | G | A | g | c | c | 6 | 4 | 8 | c | false | <90% | 7327 | ||
NM_001042495 | SLC12A6 | 1093 | 1111 | G | G | C | C | A | U | C | u | A | U | G | C | U | G | G | A | g | c | c | 6 | 4 | 8 | c | false | <90% | 7363 | ||
NM_001042497 | SLC12A6 | 1187 | 1205 | G | G | C | C | A | U | C | u | A | U | G | C | U | G | G | A | g | c | c | 6 | 4 | 8 | c | false | <90% | 7457 | ||
NM_133647 | SLC12A6 | 1232 | 1250 | G | G | C | C | A | U | C | u | A | U | G | C | U | G | G | A | g | c | c | 6 | 4 | 8 | c | false | <90% | 7502 | ||
NM_001042494 | SLC12A6 | 1418 | 1436 | G | G | C | C | A | U | C | u | A | U | G | C | U | G | G | A | g | c | c | 6 | 4 | 8 | c | false | <90% | 7688 | ||
NM_001042496 | SLC12A6 | 1484 | 1502 | G | G | C | C | A | U | C | u | A | U | G | C | U | G | G | A | g | c | c | 6 | 4 | 8 | c | false | <90% | 7754 | ||
NM_018484 | SLC22A11 | 510 | 528 | G | G | C | C | A | c | C | G | A | U | G | C | U | G | G | A | c | a | c | 5 | 4 | 10 | c | false | <90% | 2545 | ||
NM_007130 | ZNF41 | 657 | 675 | G | G | C | C | A | U | g | G | A | U | G | C | U | G | G | A | g | g | g | 2 | 4 | 9 | c | false | <90% | 3172 | ||
NM_153380 | ZNF41 | 891 | 909 | G | G | C | C | A | U | g | G | A | U | G | C | U | G | G | A | g | g | g | 2 | 4 | 9 | c | false | <90% | 3406 | ||
XM_944389 | TMEM131 | 174 | 192 | a | G | C | C | A | c | C | a | A | U | G | C | U | G | G | A | U | U | U | -27 | 3 | 11 | bc | false | <90% | 6257 | ||
XM_371542 | TMEM131 | 174 | 192 | a | G | C | C | A | c | C | a | A | U | G | C | U | G | G | A | U | U | U | -27 | 3 | 11 | bc | false | <90% | 6257 | ||
XM_939932 | TMEM131 | 571 | 589 | a | G | C | C | A | c | C | a | A | U | G | C | U | G | G | A | U | U | U | -27 | 3 | 11 | bc | false | <90% | 6654 |
Hits to Sense strand
Accession | Gene | Start | End | Details of mismatches | Spec. Score | Mismatches | Contiguous | Jackson style specificity | Hits similar gene | HSP Score | HSP % Identity | HSP length | Gene Length | ||||||||||||||||||
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | |||||||||||||
NM_000991 | RPL28 | 1565 | 1583 | g | g | A | U | C | C | A | G | C | A | U | C | c | A | U | G | G | C | a | 71 | 4 | 10 | false | <90% | 4243 | |||
NM_016298 | FBXO40 | 1637 | 1655 | g | g | u | U | C | C | A | G | C | A | U | C | G | A | U | G | c | C | C | 43 | 4 | 13 | c | false | <90% | 3440 | ||
XR_018252 | LOC347422 | 111 | 129 | g | A | A | U | C | C | A | G | C | A | g | C | G | A | U | G | G | a | g | 29 | 4 | 9 | c | false | <90% | 1988 | ||
XR_016128 | LOC347422 | 111 | 129 | g | A | A | U | C | C | A | G | C | A | g | C | G | A | U | G | G | a | g | 29 | 4 | 9 | c | false | <90% | 1988 | ||
NM_001565 | CXCL10 | 293 | 311 | g | A | A | U | C | C | A | G | a | A | U | C | G | A | a | G | G | C | C | 13 | 3 | 7 | c | false | <90% | 1172 | ||
XM_001127580 | LOC728505 | 618 | 636 | A | A | A | U | u | C | A | G | C | A | a | C | G | A | U | G | G | C | a | -17 | 3 | 7 | c | false | <90% | 965 | ||
XM_001126984 | LOC730735 | 618 | 636 | A | A | A | U | u | C | A | G | C | A | a | C | G | A | U | G | G | C | a | -17 | 3 | 7 | c | false | <90% | 965 |
3 prime UTR Seed matches
Hits to same gene : 0
Hits to other genes : 1403
View matched accessions
3 prime seed match details
Immunostimulatory motif search results
UGUGU - Judge et al. Sequence-dependent stimulation of the mammalian innate immune response by synthetic siRNA. Nat Biotechnol. 2005 Apr;23(4):457-62. (PMID: 15778705)
No Immunostimulatory motif found (UGUGU).
GUCCUUCAA - Hornung et al. Sequence-specific potent induction of IFN-alpha by short interfering RNA in plasmacytoid dendritic cells through TLR7. Nat Med. 2005 Mar;11(3):263-70. Epub 2005 Feb 20. (PMID: 15723075)
No Immunostimulatory motif found (GUCCUUCAA).
Settings used for search
Scores were calculated using scoring scheme SS1
Using AVG scoring mode.
Hit scores
Parameter | Spec. Score cut-off | Colour | Comment |
Hit to same gene | Colour | Hits to the same accession or genes with 100 percent identity (i.e. likely transcript variants). It is advisable to always check the genes hit. | |
High scoring hit | ≥80 | Colour | Hits are very likely to cause an off-target effect |
Medium scoring hit | <80¸≥40 | Colour | Hits with a small chance of causing an off target effect. |
Low scoring hit | Colour | Hits very unlikely to cause an off-target effect |
The mismatches are colour coded based on the weighting of that position.
Cut-off | Colour |
Match | Colour |
Wobble base pairs (G-U) | Colour |
Critical bases (none) | Colour |
0.6 bits | Colour |
0.4 bits | Colour |
0.2 bits | Colour |
SpecificityServer is designed to help you identify potential non-specific matches to your siRNA. It incorporates the latest information about non-specific matches (sequence-specific only).
Designed by: Alistair Chalk, Quan Du, Zicai Liang and Erik L.L. Sonnhammer