Automatic recommendation
Warning - siRNA is specific for only the antisense (guide) strand
57 hits were found for GGUACAACAUCAUCAACUU (33 guide strand hits and 24 sense strand hits.)
Hits to Guide strand
Accession | Gene | Start | End | Details of mismatches | Spec. Score | Mismatches | Contiguous | Jackson style specificity | Hits similar gene | HSP Score | HSP % Identity | HSP length | Gene Length | ||||||||||||||||||
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | |||||||||||||
NR_001282 | PCDHB19P | 1066 | 1084 | a | G | U | A | C | A | A | C | A | U | C | A | c | C | A | A | C | a | c | 79 | 4 | 11 | false | <90% | 4613 | |||
NM_001103 | ACTN2 | 2218 | 2236 | a | G | c | A | C | A | A | C | A | U | C | A | U | C | A | A | C | U | a | 73 | 3 | 15 | bc | false | <90% | 4181 | ||
NM_002208 | ITGAE | 1273 | 1291 | G | G | U | A | C | A | A | C | A | U | C | A | U | C | A | g | C | a | U | 71 | 2 | 15 | c | false | <90% | 3927 | ||
NM_022111 | CLSPN | 4318 | 4336 | u | u | U | A | C | A | A | C | A | U | C | A | a | C | A | A | C | U | c | 71 | 4 | 10 | false | <90% | 4379 | |||
NM_138318 | KCNK10 | 1456 | 1474 | a | G | g | A | C | A | A | C | A | U | C | A | U | C | A | A | C | a | a | 70 | 4 | 14 | c | false | <90% | 2401 | ||
NM_138317 | KCNK10 | 1539 | 1557 | a | G | g | A | C | A | A | C | A | U | C | A | U | C | A | A | C | a | a | 70 | 4 | 14 | c | false | <90% | 2484 | ||
NM_021161 | KCNK10 | 1771 | 1789 | a | G | g | A | C | A | A | C | A | U | C | A | U | C | A | A | C | a | a | 70 | 4 | 14 | c | false | <90% | 2710 | ||
XM_942459 | LOC652798 | 1625 | 1643 | u | c | a | A | C | A | A | C | A | U | C | A | U | C | A | A | C | c | U | 64 | 4 | 14 | c | false | <90% | 1815 | ||
NM_020393 | PGLYRP4 | 1147 | 1165 | u | G | U | A | C | A | A | C | A | U | C | A | U | C | A | g | C | a | c | 64 | 4 | 14 | c | false | <90% | 1853 | ||
NM_001005337 | PKP1 | 2242 | 2260 | u | c | a | A | C | A | A | C | A | U | C | A | U | C | A | A | C | c | U | 64 | 4 | 14 | c | false | <90% | 5388 | ||
NM_000299 | PKP1 | 2305 | 2323 | u | c | a | A | C | A | A | C | A | U | C | A | U | C | A | A | C | c | U | 64 | 4 | 14 | c | false | <90% | 5451 | ||
XM_001126252 | LOC727965 | 431 | 449 | G | c | a | A | C | A | A | C | A | U | C | A | U | C | A | A | C | a | c | 61 | 4 | 14 | c | false | <90% | 843 | ||
NM_212539 | PRKCD | 1269 | 1287 | a | G | U | g | C | A | A | C | A | U | C | A | a | C | A | A | C | U | U | 50 | 3 | 8 | false | <90% | 2738 | |||
NM_006254 | PRKCD | 1381 | 1399 | a | G | U | g | C | A | A | C | A | U | C | A | a | C | A | A | C | U | U | 50 | 3 | 8 | false | <90% | 2850 | |||
NM_152336 | AGBL1 | 1882 | 1900 | a | c | U | u | C | A | A | C | A | U | C | A | U | C | A | A | C | U | g | 44 | 4 | 14 | bc | false | <90% | 3493 | ||
NM_005955 | MTF1 | 161 | 179 | a | c | a | A | C | A | A | C | A | U | C | A | U | C | u | A | C | U | U | 42 | 4 | 11 | c | false | <90% | 7986 | ||
XR_016445 | LOC392538 | 730 | 748 | a | G | a | u | C | A | A | C | A | U | C | A | U | C | A | A | C | U | c | 36 | 4 | 14 | bc | false | <90% | 914 | ||
XR_018555 | LOC392538 | 730 | 748 | a | G | a | u | C | A | A | C | A | U | C | A | U | C | A | A | C | U | c | 36 | 4 | 14 | bc | false | <90% | 914 | ||
NM_017789 | SEMA4C | 1293 | 1311 | c | c | g | A | C | A | A | C | A | U | C | c | U | C | A | A | C | U | U | 33 | 4 | 8 | false | <90% | 3537 | |||
NM_017539 | DNAH3 | 5225 | 5243 | a | G | U | A | C | A | A | g | A | U | C | A | U | C | A | A | C | c | c | 28 | 4 | 9 | c | false | <90% | 12351 | ||
XR_015266 | LOC286467 | 646 | 664 | u | G | U | A | C | A | c | C | A | U | C | A | U | C | A | A | C | g | g | 24 | 4 | 10 | c | false | <90% | 1466 | ||
XR_015395 | LOC286467 | 646 | 664 | u | G | U | A | C | A | c | C | A | U | C | A | U | C | A | A | C | g | g | 24 | 4 | 10 | c | false | <90% | 1466 | ||
NM_053067 | UBQLN1 | 653 | 671 | G | u | U | A | C | u | A | C | A | U | C | A | U | C | A | A | C | U | c | 20 | 3 | 12 | bc | false | <90% | 2354 | ||
NM_013438 | UBQLN1 | 738 | 756 | G | u | U | A | C | u | A | C | A | U | C | A | U | C | A | A | C | U | c | 20 | 3 | 12 | bc | false | <90% | 3952 | ||
NM_002556 | OSBP | 3398 | 3416 | G | G | U | A | u | A | A | C | A | U | C | A | U | C | A | A | g | U | g | 20 | 3 | 11 | c | false | <90% | 5083 | ||
NM_020202 | NIT2 | 943 | 961 | u | c | U | A | C | A | A | C | A | U | a | A | U | C | A | A | C | U | c | 20 | 4 | 8 | c | false | <90% | 1016 | ||
NM_015885 | PCF11 | 3179 | 3197 | G | G | g | A | C | A | A | C | A | U | a | A | U | C | A | A | C | U | U | 19 | 2 | 8 | c | false | <90% | 5834 | ||
NM_015071 | ARHGAP26 | 1882 | 1900 | G | G | a | A | C | A | g | C | A | U | C | A | U | C | A | A | C | U | c | 9 | 3 | 11 | bc | false | <90% | 9035 | ||
NM_002971 | SATB1 | 2302 | 2320 | a | G | U | A | C | A | c | C | A | U | C | A | U | C | A | A | g | U | U | 8 | 3 | 9 | c | false | <90% | 3782 | ||
NM_001077501 | LRRCC1 | 1670 | 1688 | G | c | a | A | C | A | A | C | u | U | C | A | U | C | A | A | C | U | U | 8 | 3 | 10 | bc | false | <90% | 3619 | ||
NM_033402 | LRRCC1 | 1876 | 1894 | G | c | a | A | C | A | A | C | u | U | C | A | U | C | A | A | C | U | U | 8 | 3 | 10 | bc | false | <90% | 3825 | ||
XM_941646 | LOC649371 | 216 | 234 | u | G | U | A | C | g | A | C | A | U | C | u | U | C | A | A | C | U | U | 0 | 3 | 7 | false | <90% | 1092 | |||
NM_170776 | GPR97 | 202 | 220 | u | G | U | A | C | g | A | C | A | U | C | u | U | C | A | A | C | U | U | 0 | 3 | 7 | false | <90% | 2681 |
Hits to Sense strand
Accession | Gene | Start | End | Details of mismatches | Spec. Score | Mismatches | Contiguous | Jackson style specificity | Hits similar gene | HSP Score | HSP % Identity | HSP length | Gene Length | ||||||||||||||||||
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | |||||||||||||
XM_939560 | NBPF7 | 135 | 153 | u | g | G | U | U | G | A | U | G | A | U | G | U | U | G | U | A | C | a | 81 | 3 | 16 | abc | false | <90% | 1887 | ||
NM_001047980 | NBPF7 | 135 | 153 | u | g | G | U | U | G | A | U | G | A | U | G | U | U | G | U | A | C | a | 81 | 3 | 16 | abc | false | <90% | 1887 | ||
NM_007202 | AKAP10 | 1493 | 1511 | g | A | u | U | U | G | A | U | G | A | U | G | U | U | G | U | A | C | g | 73 | 3 | 15 | bc | false | <90% | 2929 | ||
NM_017440 | MDM1 | 2608 | 2626 | u | A | a | U | U | G | A | U | G | A | U | G | U | U | G | U | A | a | a | 70 | 4 | 14 | c | false | <90% | 2936 | ||
XR_015405 | LOC728895 | 327 | 345 | g | c | c | U | U | G | A | U | G | A | U | G | U | U | G | U | A | C | a | 62 | 4 | 15 | bc | false | <90% | 1819 | ||
XM_001133092 | LOC728912 | 584 | 602 | g | c | c | U | U | G | A | U | G | A | U | G | U | U | G | U | A | C | a | 62 | 4 | 15 | bc | false | <90% | 2950 | ||
XM_001133097 | LOC728912 | 584 | 602 | g | c | c | U | U | G | A | U | G | A | U | G | U | U | G | U | A | C | a | 62 | 4 | 15 | bc | false | <90% | 3194 | ||
XM_001133188 | LOC728936 | 68 | 86 | g | c | c | U | U | G | A | U | G | A | U | G | U | U | G | U | A | C | a | 62 | 4 | 15 | bc | false | <90% | 4492 | ||
XM_001133155 | LOC728936 | 589 | 607 | g | c | c | U | U | G | A | U | G | A | U | G | U | U | G | U | A | C | a | 62 | 4 | 15 | bc | false | <90% | 4784 | ||
XM_001133176 | LOC728936 | 589 | 607 | g | c | c | U | U | G | A | U | G | A | U | G | U | U | G | U | A | C | a | 62 | 4 | 15 | bc | false | <90% | 4898 | ||
XM_001133169 | LOC728936 | 589 | 607 | g | c | c | U | U | G | A | U | G | A | U | G | U | U | G | U | A | C | a | 62 | 4 | 15 | bc | false | <90% | 4922 | ||
XM_001133179 | LOC728936 | 589 | 607 | g | c | c | U | U | G | A | U | G | A | U | G | U | U | G | U | A | C | a | 62 | 4 | 15 | bc | false | <90% | 5093 | ||
XM_001133191 | LOC728936 | 589 | 607 | g | c | c | U | U | G | A | U | G | A | U | G | U | U | G | U | A | C | a | 62 | 4 | 15 | bc | false | <90% | 5111 | ||
XR_015220 | LOC730476 | 641 | 659 | g | c | c | U | U | G | A | U | G | A | U | G | U | U | G | U | A | C | a | 62 | 4 | 15 | bc | false | <90% | 5174 | ||
XM_001133181 | LOC728936 | 589 | 607 | g | c | c | U | U | G | A | U | G | A | U | G | U | U | G | U | A | C | a | 62 | 4 | 15 | bc | false | <90% | 5622 | ||
XR_015592 | LOC729561 | 1070 | 1088 | A | A | G | U | U | G | A | U | G | A | U | G | a | U | G | U | u | u | a | 58 | 4 | 12 | false | <90% | 1478 | |||
XR_015583 | LOC731731 | 1085 | 1103 | A | A | G | U | U | G | A | U | G | A | U | G | a | U | G | U | u | u | a | 58 | 4 | 12 | false | <90% | 1493 | |||
NM_001047160 | NET1 | 352 | 370 | A | A | G | a | U | G | A | U | G | A | U | G | U | U | G | U | A | a | g | 55 | 3 | 13 | c | false | <90% | 3414 | ||
NM_001644 | APOBEC1 | 553 | 571 | c | u | G | U | g | G | A | U | G | A | U | G | U | U | G | U | A | C | g | 30 | 4 | 13 | bc | false | <90% | 911 | ||
XM_001131192 | LOC646520 | 553 | 571 | A | A | G | U | U | G | A | U | G | A | U | G | U | U | a | a | g | g | C | 23 | 4 | 14 | c | false | <90% | 690 | ||
NM_020792 | AADACL1 | 705 | 723 | A | A | a | U | U | c | A | U | G | A | U | G | U | U | G | U | A | C | g | 12 | 3 | 12 | bc | false | <90% | 4289 | ||
NM_001081676 | SCN3A | 3775 | 3793 | c | A | G | U | U | G | A | U | G | u | U | G | U | U | c | U | A | C | C | -9 | 3 | 8 | c | false | <90% | 8994 | ||
NM_001081677 | SCN3A | 3775 | 3793 | c | A | G | U | U | G | A | U | G | u | U | G | U | U | c | U | A | C | C | -9 | 3 | 8 | c | false | <90% | 8994 | ||
NM_006922 | SCN3A | 3922 | 3940 | c | A | G | U | U | G | A | U | G | u | U | G | U | U | c | U | A | C | C | -9 | 3 | 8 | c | false | <90% | 9141 |
3 prime UTR Seed matches
Hits to same gene : 0
Hits to other genes : 1022
View matched accessions
3 prime seed match details
Immunostimulatory motif search results
UGUGU - Judge et al. Sequence-dependent stimulation of the mammalian innate immune response by synthetic siRNA. Nat Biotechnol. 2005 Apr;23(4):457-62. (PMID: 15778705)
No Immunostimulatory motif found (UGUGU).
GUCCUUCAA - Hornung et al. Sequence-specific potent induction of IFN-alpha by short interfering RNA in plasmacytoid dendritic cells through TLR7. Nat Med. 2005 Mar;11(3):263-70. Epub 2005 Feb 20. (PMID: 15723075)
No Immunostimulatory motif found (GUCCUUCAA).
Settings used for search
Scores were calculated using scoring scheme SS1
Using AVG scoring mode.
Hit scores
Parameter | Spec. Score cut-off | Colour | Comment |
Hit to same gene | Colour | Hits to the same accession or genes with 100 percent identity (i.e. likely transcript variants). It is advisable to always check the genes hit. | |
High scoring hit | ≥80 | Colour | Hits are very likely to cause an off-target effect |
Medium scoring hit | <80¸≥40 | Colour | Hits with a small chance of causing an off target effect. |
Low scoring hit | Colour | Hits very unlikely to cause an off-target effect |
The mismatches are colour coded based on the weighting of that position.
Cut-off | Colour |
Match | Colour |
Wobble base pairs (G-U) | Colour |
Critical bases (none) | Colour |
0.6 bits | Colour |
0.4 bits | Colour |
0.2 bits | Colour |
SpecificityServer is designed to help you identify potential non-specific matches to your siRNA. It incorporates the latest information about non-specific matches (sequence-specific only).
Designed by: Alistair Chalk, Quan Du, Zicai Liang and Erik L.L. Sonnhammer