VIRsiRNAdb

SpecificityServer - An siRNA design program for determining siRNA specificity
Automatic recommendation
Not OK - siRNA is not specific

49 hits were found for CGGACACACAAAAAGAAAG (26 guide strand hits and 23 sense strand hits.)

Hits to Guide strand

Accession

Gene

Start

End

Details of mismatches

Spec. Score

Mismatches

Contiguous

Jackson style specificity

Hits similar gene

HSP Score

HSP % Identity

HSP length

Gene Length

1

2

3

4

5

6

7

8

9

10

11

12

13

14

15

16

17

18

19

NM_006425

SLU7

531

549

a

u

G

A

C

A

C

A

C

A

A

A

A

A

G

A

A

A

G

86

2

17

abc

false

<90%

3570

NM_198175

NME1

169

187

C

a

G

A

C

A

C

A

C

A

A

A

c

A

G

A

A

A

a

73

3

10

false

<90%

1031

NM_017738

C9orf39

1105

1123

u

G

G

A

C

A

C

A

C

A

A

A

A

A

G

u

A

c

u

64

4

14

c

false

<90%

5522

NM_000389

CDKN1A

1503

1521

g

G

G

A

C

A

C

A

C

A

A

g

A

A

G

A

A

g

G

61

3

10

false

<90%

2140

NM_078467

CDKN1A

1643

1661

g

G

G

A

C

A

C

A

C

A

A

g

A

A

G

A

A

g

G

61

3

10

false

<90%

2281

NM_001006684

TCEAL8

959

977

C

u

c

A

C

A

C

A

C

A

A

A

A

A

G

A

A

u

u

61

4

14

c

false

<90%

1158

NM_153333

TCEAL8

1032

1050

C

u

c

A

C

A

C

A

C

A

A

A

A

A

G

A

A

u

u

61

4

14

c

false

<90%

1231

NM_001031730

TSPAN18

1166

1184

a

u

G

A

C

A

C

A

C

A

A

A

A

A

c

A

A

A

a

56

4

12

c

false

<90%

1198

NM_207645

LOC399947

2329

2347

a

u

G

A

C

A

C

A

C

A

A

A

A

A

a

A

A

A

a

56

4

12

c

false

<90%

2353

NM_016284

CNOT1

8422

8440

a

u

G

A

C

A

C

A

C

A

A

A

A

A

a

A

A

A

a

56

4

12

c

false

<90%

8449

NM_031466

NIBP

1966

1984

C

G

G

c

C

A

C

A

C

A

A

A

A

u

G

A

A

A

a

38

3

9

c

false

<90%

4472

XR_018312

LOC440180

1509

1527

a

a

G

A

a

A

C

A

C

A

A

A

A

A

G

A

A

A

u

30

4

13

bc

false

<90%

1817

XR_017569

LOC440180

1509

1527

a

a

G

A

a

A

C

A

C

A

A

A

A

A

G

A

A

A

u

30

4

13

bc

false

<90%

1817

NM_032226

ZCCHC7

1504

1522

a

a

G

A

a

A

C

A

C

A

A

A

A

A

G

A

A

A

u

30

4

13

bc

false

<90%

2701

NM_022817

PER2

551

569

u

G

G

A

C

A

C

A

C

A

c

A

A

A

G

A

A

c

u

29

4

9

c

false

<90%

6342

NM_198483

RUFY4

2316

2334

g

G

G

A

C

u

C

A

C

A

A

A

A

A

G

g

A

A

G

8

3

9

c

false

<90%

3567

NM_014309

RBM9

1583

1601

a

a

a

A

C

A

C

A

a

A

A

A

A

A

G

A

A

A

G

6

4

10

bc

false

<90%

6948

NM_001082579

RBM9

1589

1607

a

a

a

A

C

A

C

A

a

A

A

A

A

A

G

A

A

A

G

6

4

10

bc

false

<90%

6954

NM_001082577

RBM9

1592

1610

a

a

a

A

C

A

C

A

a

A

A

A

A

A

G

A

A

A

G

6

4

10

bc

false

<90%

6957

NM_001082578

RBM9

1592

1610

a

a

a

A

C

A

C

A

a

A

A

A

A

A

G

A

A

A

G

6

4

10

bc

false

<90%

6957

NM_001082576

RBM9

1620

1638

a

a

a

A

C

A

C

A

a

A

A

A

A

A

G

A

A

A

G

6

4

10

bc

false

<90%

6985

NM_001031695

RBM9

1632

1650

a

a

a

A

C

A

C

A

a

A

A

A

A

A

G

A

A

A

G

6

4

10

bc

false

<90%

6997

NM_015446

AHCTF1

6615

6633

a

a

a

A

C

A

C

A

g

A

A

A

A

A

G

A

A

A

G

6

4

10

bc

false

<90%

8526

XM_001126456

AHCTF1

6611

6629

a

a

a

A

C

A

C

A

g

A

A

A

A

A

G

A

A

A

G

6

4

10

bc

false

<90%

8526

NM_007344

TTF1

2676

2694

a

G

G

A

C

A

u

A

g

A

A

A

A

A

G

A

A

A

G

-28

3

10

bc

false

<90%

3137

NM_003750

EIF3S10

3754

3772

g

G

G

A

C

A

g

A

g

A

A

A

A

A

G

A

A

A

G

-28

3

10

bc

false

<90%

5305

Hits to Sense strand

Accession

Gene

Start

End

Details of mismatches

Spec. Score

Mismatches

Contiguous

Jackson style specificity

Hits similar gene

HSP Score

HSP % Identity

HSP length

Gene Length

1

2

3

4

5

6

7

8

9

10

11

12

13

14

15

16

17

18

19

NM_014166

MED4

1255

1273

a

U

U

U

C

U

U

U

U

U

G

U

G

U

G

U

C

a

u

90

3

16

ac

false

<90%

2022

NM_014050

MRPL42

1050

1068

a

U

c

U

C

U

U

U

U

U

G

U

G

U

G

U

C

u

a

70

4

14

c

false

<90%

2070

NM_172177

MRPL42

1053

1071

a

U

c

U

C

U

U

U

U

U

G

U

G

U

G

U

C

u

a

70

4

14

c

false

<90%

2073

NM_172178

MRPL42

1073

1091

a

U

c

U

C

U

U

U

U

U

G

U

G

U

G

U

C

u

a

70

4

14

c

false

<90%

2093

NM_015020

PHLPPL

4058

4076

a

U

U

U

C

U

U

U

U

U

G

U

u

U

G

U

u

C

G

64

3

11

false

<90%

7919

NM_013402

FADS1

4129

4147

u

a

U

U

C

U

U

U

U

U

G

U

G

U

G

U

g

C

c

58

4

14

ac

false

<90%

4213

NM_001915

CYB561

2285

2303

C

U

U

U

C

U

U

U

U

U

G

g

G

U

G

U

u

u

G

40

3

11

false

<90%

3003

NM_001017917

CYB561

2288

2306

C

U

U

U

C

U

U

U

U

U

G

g

G

U

G

U

u

u

G

40

3

11

false

<90%

3006

NM_001017916

CYB561

2455

2473

C

U

U

U

C

U

U

U

U

U

G

g

G

U

G

U

u

u

G

40

3

11

false

<90%

3173

NM_014225

PPP2R1A

2238

2256

g

U

U

U

u

U

U

U

U

U

G

U

G

U

G

U

C

a

a

38

4

12

c

false

<90%

2357

NM_033626

CCDC120

2457

2475

C

U

U

U

C

U

U

U

U

U

c

U

G

U

G

U

C

u

u

31

3

10

c

false

<90%

4004

NM_014243

ADAMTS3

4553

4571

C

U

U

U

g

U

U

U

U

U

G

U

G

U

G

U

g

u

G

22

3

11

c

false

<90%

5821

NM_001080519

BAHCC1

10453

10471

C

U

U

U

C

U

U

U

U

U

c

U

G

U

G

U

g

C

G

15

2

10

c

false

<90%

10708

NM_012099

CD3EAP

3077

3095

C

U

U

U

C

U

U

U

g

U

G

U

G

U

G

U

g

u

G

13

3

8

c

false

<90%

3286

NM_022652

DUSP6

1263

1281

C

U

U

U

C

U

U

U

g

U

G

U

G

U

G

g

C

C

c

8

3

8

c

false

<90%

2404

NM_001946

DUSP6

1701

1719

C

U

U

U

C

U

U

U

g

U

G

U

G

U

G

g

C

C

c

8

3

8

c

false

<90%

2842

NM_004404

SEPT2

2587

2605

C

U

U

U

C

U

U

c

U

U

G

U

G

U

c

U

C

C

u

8

3

7

c

false

<90%

3311

NM_001008492

SEPT2

2672

2690

C

U

U

U

C

U

U

c

U

U

G

U

G

U

c

U

C

C

u

8

3

7

c

false

<90%

3396

NM_006155

SEPT2

2773

2791

C

U

U

U

C

U

U

c

U

U

G

U

G

U

c

U

C

C

u

8

3

7

c

false

<90%

3497

NM_001008491

SEPT2

2874

2892

C

U

U

U

C

U

U

c

U

U

G

U

G

U

c

U

C

C

u

8

3

7

c

false

<90%

3598

NM_033656

BRWD1

9771

9789

C

U

U

U

C

U

U

U

U

U

u

c

G

U

G

U

C

C

u

0

3

10

false

<90%

13003

NM_001010861

PAP2D

1361

1379

C

U

U

U

u

U

U

U

U

U

c

U

G

U

G

U

C

C

c

-17

3

7

c

false

<90%

4008

NM_001037317

PAP2D

1376

1394

C

U

U

U

u

U

U

U

U

U

c

U

G

U

G

U

C

C

c

-17

3

7

c

false

<90%

4023

---

3 prime UTR Seed matches

Hits to same gene : 0
Hits to other genes : 419
View matched accessions

3 prime seed match details

Accession	Gene name	Hits to this gene
XM_936719	LOC647662	5
NM_001004358	FGFRL1	4
NM_021923	FGFRL1	4
NM_001004356	FGFRL1	4
NM_175895	C12orf61	2
NM_015627	LDLRAP1	2
NM_005187	CBFA2T3	2
NM_175931	CBFA2T3	2
NM_182562	FLJ39743	2
NM_005772	RCL1	2
NM_152346	SLC43A2	2
NM_002698	POU2F2	2
NM_005860	FSTL3	2
NM_001026213	CYP11B1	1
NM_000497	CYP11B1	1
NM_003950	F2RL3	1
NM_018121	C10orf6	1
NM_001042682	RERE	1
NM_012102	RERE	1
NM_001042681	RERE	1
NM_005263	GFI1	1
NM_014112	TRPS1	1
NM_023004	RTN4R	1
XM_001128832	LOC730625	1
XM_001133424	LOC729224	1
NM_032595	PPP1R9B	1
NM_020956	PRX	1
NM_174953	ATP2A3	1
NM_174956	ATP2A3	1
NM_174954	ATP2A3	1
NM_174957	ATP2A3	1
NM_174958	ATP2A3	1
NM_005173	ATP2A3	1
NM_174955	ATP2A3	1
XM_001125753	MUC3B	1
XM_001131827	MUC3B	1
NM_005205	COX6A2	1
NM_004907	IER2	1
NM_032977	CASP10	1
NM_001230	CASP10	1
NM_003430	ZNF91	1
NM_030761	WNT4	1
NM_023112	OTUB2	1
NM_152536	FGD5	1
NM_001007271	DUSP13	1
NM_018436	ALLC	1
NM_002695	POLR2E	1
NM_015934	NOP5/NOP58	1
NM_013305	ST8SIA5	1
NM_013433	TNPO2	1
NM_001204	BMPR2	1
XM_928199	LOC400581	1
NM_017720	STAP2	1
NM_001013841	STAP2	1
NM_020698	TMCC3	1
NM_003180	SYT5	1
NM_030818	CCDC130	1
NM_003281	TNNI1	1
NM_000913	OPRL1	1
NM_182647	OPRL1	1
NM_002688	SEPT5	1
NM_198183	UBE2L6	1
NM_004223	UBE2L6	1
NM_001077511	TCF19	1
NM_007109	TCF19	1
NM_057170	GIT2	1
NM_057169	GIT2	1
NM_014776	GIT2	1
NM_032864	PRPF38A	1
NM_014077	FAM32A	1
NM_014015	DEXI	1
XM_926382	LOC642755	1
NM_198829	RAC1	1
NM_006908	RAC1	1
NM_018890	RAC1	1
NM_001299	CNN1	1
NM_198458	ZNF497	1
NM_014460	CSDC2	1
NM_139278	LGI3	1
NM_005766	FARP1	1
NM_022062	PKNOX2	1
NM_002978	SCNN1D	1
NM_002340	LSS	1
NM_001008392	CTDSPL	1
NM_005808	CTDSPL	1
NM_183361	DTNB	1
NM_021907	DTNB	1
NM_033147	DTNB	1
NM_033148	DTNB	1
NM_183360	DTNB	1
NM_005400	PRKCE	1
NM_181553	CMTM3	1
NM_001048251	CMTM3	1
NM_181554	CMTM3	1
NM_144601	CMTM3	1
NM_018958	C15orf2	1
NM_005076	CNTN2	1
NM_015995	KLF13	1
NM_001420	ELAVL3	1
NM_032281	ELAVL3	1
NM_004745	DLGAP2	1
NM_002360	MAFK	1
NM_001042690	LOC345222	1
NM_144564	SLC39A3	1
NM_024833	ZNF671	1
NM_174914	UGT3A2	1
NM_199205	KIAA1967	1
NM_021174	KIAA1967	1
NM_183048	ZMYND8	1
NM_183047	ZMYND8	1
NM_012408	ZMYND8	1
NM_003120	SPI1	1
NM_001080547	SPI1	1
NM_020946	DENND1A	1
NM_145257	C1orf96	1
NM_172212	CSF1	1
NM_020205	OTUD7B	1
NM_002797	PSMB5	1
XM_932965	LOC645553	1
XM_945010	LOC645553	1
NM_001859	SLC31A1	1
NM_001918	DBT	1
NM_012306	FAIM2	1
NM_006180	NTRK2	1
NM_001018064	NTRK2	1
NM_152219	GJC1	1
NM_001102402		1
NM_021213	PCTP	1
NM_001080396	LOC728215	1
XM_001133620	LOC731895	1
NM_020742	NLGN4X	1
NM_181332	NLGN4X	1
NM_003482	MLL2	1
NM_014572	LATS2	1
NM_014021	SSX2IP	1
NM_173575	STK32C	1
NM_001039618	CREBZF	1
NM_017911	FAM118A	1
NM_001104595		1
NM_025237	SOST	1
NM_014141	CNTNAP2	1
NM_003592	CUL1	1
XM_001128325	LOC283481	1
XM_001133584	LOC283481	1
NM_000135	FANCA	1
NM_000599	IGFBP5	1
NM_173065	IL28RA	1
NM_173064	IL28RA	1
NM_170743	IL28RA	1
NM_006875	PIM2	1
NM_178812	MTDH	1
NM_152833		1
NM_138783	ZNF653	1
NM_007030	TPPP	1
NM_152308	C16orf75	1
NM_024830	AYTL2	1
NM_001012418	LOC340156	1
NM_016458	C8orf30A	1
XM_001126758	LOC728071	1
NM_022134	GAL3ST2	1
NM_178013	PRIMA1	1
NM_001128	AP1G1	1
NM_001030007	AP1G1	1
NM_144993		1
NM_001035235	SRA1	1
NM_001043	SLC6A2	1
NM_000033	ABCD1	1
XM_930750	LOC642366	1
XM_943652	LOC642366	1
NM_145239	PRRT2	1
NM_015341	NCAPH	1
NM_002829	PTPN3	1
NM_001098798		1
NM_001098797		1
NM_001098796		1
NM_032883	TOX2	1
NM_006532	ELL	1
NM_030578	MGC4093	1
NM_002309	LIF	1
NM_001033602	KIAA0774	1
NM_015233	KIAA0774	1
NM_004095	EIF4EBP1	1
NM_002229	JUNB	1
XM_001126328	LOC388152	1
NM_003670	BHLHB2	1
NM_001001795	MGC70857	1
NM_014213	HOXD9	1
NM_007174	CIT	1
NM_007079	PTP4A3	1
NM_032611	PTP4A3	1
NM_006160	NEUROD2	1
NM_207391	RGS9BP	1
NM_022124	CDH23	1
NM_020908		1
NM_005078	TLE3	1
NM_001105192		1
NM_174940	TMEM80	1
NM_001042463	TMEM80	1
NM_001010898	SLC6A17	1
NM_016406	UFC1	1
NM_032482	DOT1L	1
NM_024509	LRFN3	1
NM_005652	TERF2	1
NM_015101	GLT25D2	1
NM_001098721		1
NM_001098722		1
NM_004485	GNG4	1
NM_001099857		1
NM_003639	IKBKG	1
NM_001099856		1
NM_130848	C5orf20	1
NM_015694	ZNF777	1
NM_145173	DIRAS1	1
XM_943278	LOC644727	1
XM_932334	LOC644727	1
NM_001001938	C9orf47	1
XM_379029	LOC400879	1
NM_013449	BAZ2A	1
NM_005185	CALML3	1
NM_013261	PPARGC1A	1
NM_181552	CUTL1	1
NM_033064	ATCAY	1
NM_033044	MACF1	1
NM_012090	MACF1	1
NM_001042646	TRAK1	1
NM_003225	TFF1	1
NM_014742	TM9SF4	1
NM_006861	RAB35	1
NM_013937	OR11A1	1
NM_001099784		1
NM_020972	ZFYVE28	1
NM_001017995	SH3PXD2B	1
NM_178520	TMEM105	1
NM_006291	TNFAIP2	1
NM_002359	MAFG	1
NM_032711	MAFG	1
NM_181846	ZSCAN22	1
NM_016734	PAX5	1
NM_206920	MAMDC4	1
NM_001077418	FLJ22167	1
NM_001077416	FLJ22167	1
NM_001077419	FLJ22167	1
NM_173832	ZFP41	1
NM_005663	WHSC2	1
NM_006591	POLD3	1
NM_017827	SARS2	1
NM_153329	ALDH16A1	1
XM_001133339	LOC731569	1
XM_001133506	LOC729534	1
NM_144599	NIPA1	1
NM_002531	NTSR1	1
NM_001037666	LOC652968	1
NM_144659	TCP10L	1
NM_014494	TNRC6A	1
NM_001286	CLCN6	1
NM_153812	PHF13	1
NM_000748	CHRNB2	1
NM_024318	LILRA6	1
NM_004822	NTN1	1
NM_001080397	SLC45A1	1
XM_937695	SLC45A1	1
NM_006037	HDAC4	1
NM_182533	C1orf86	1
NM_199131	VAX1	1
NM_144991	C21orf29	1
NM_205843	NFIC	1
NM_005597	NFIC	1
NM_033259	CAMK2N2	1
NM_024653	PRKRIP1	1
NM_001039919	ZNF384	1
NM_001039917	ZNF384	1
NM_001039916	ZNF384	1
NM_001039920	ZNF384	1
NM_133476	ZNF384	1
NM_001039918	ZNF384	1
NM_024554	PGBD5	1
NM_001003676	C11orf49	1
NM_003486	SLC7A5	1
NM_004943	DMWD	1
NM_174937	TCERG1L	1
NM_198883	MTX1	1
NM_002455	MTX1	1
XM_926474	LOC642648	1
NM_182578	THEM5	1
NM_001031672	LOC606495	1
NM_002079	GOT1	1
NM_005160	ADRBK2	1
NM_001106	ACVR2B	1
NM_033201	C16orf45	1
NM_018426	TMEM63B	1
NM_021927	GUF1	1
NM_144968	RIBC1	1
NM_021163	RBAK	1
NM_015276		1
NM_014481	APEX2	1
NM_024028	PCYOX1L	1
XM_001125098	LOC730417	1
NM_002267	KPNA3	1
NM_001946	DUSP6	1
NM_022652	DUSP6	1
NM_181359	IL6R	1
NM_000565	IL6R	1
NM_018208	ETNK2	1
NM_004807	HS6ST1	1
XM_001132928	LOC728969	1
NM_194312	ESPNL	1
NM_015151	DIP2A	1
XM_001134349	LOC730176	1
NM_018206	VPS35	1
NM_014396	VPS41	1
NM_080631	VPS41	1
NM_006918	SC5DL	1
NM_001024956	SC5DL	1
NM_015316	PPP1R13B	1
NM_080764	SUHW2	1
NM_015264	C22orf9	1
NM_001009880	C22orf9	1
NM_201274	M-RIP	1
NM_015134	M-RIP	1
NM_032932	RAB11FIP4	1
NM_001076675	ZNF626	1
NM_015395	DKFZP434B0335	1
NM_005318	H1F0	1
NM_004624	VIPR1	1
NM_032836	FIZ1	1
NM_022844	MYH11	1
NM_001040113	MYH11	1
NM_004959	NR5A1	1
NM_033641	COL4A6	1
NM_001847	COL4A6	1
NM_001080543	C19orf29	1
NM_177402	SYT2	1
NM_006943	SOX12	1
NM_207322	NLF1	1
XM_940314	NLF2	1
NM_001007595	NLF2	1
NM_138353	LOC90379	1
NM_181876	PPP2R2C	1
NM_020416	PPP2R2C	1
NM_173354	SNF1LK	1
NM_001010867	C1orf69	1
NM_015059	TLN2	1
NM_021637	TMEM35	1
NM_001076682	NCAM1	1
NM_018404	CENTA2	1
NM_020910		1
NM_173541	C10orf91	1
NM_021958	HLX1	1
NM_032294	CAMKK1	1
NM_172206	CAMKK1	1
NM_001003656	PPP2R2D	1
NM_018461	PPP2R2D	1
NM_014644	PDE4DIP	1
XM_001126245	LOC727893	1
XM_001126231	LOC727893	1
NM_000115	EDNRB	1
XM_001134172	LOC730034	1
XM_001133186	LOC731345	1
NM_033551	LARP1	1
NM_015315	LARP1	1
NM_018425	PI4KII	1
NM_032630	CINP	1
NM_153481	IL17RE	1
NM_153483	IL17RE	1
NM_153480	IL17RE	1
NM_016339	RAPGEFL1	1
NM_002475	MYL6B	1
NM_004689	MTA1	1
NM_203434	IER5L	1
NM_001099691		1
NM_003236	TGFA	1
NM_020381	PDSS2	1
NM_020695	REXO1	1
NM_001430	EPAS1	1
NM_001316	CSE1L	1
NM_032781	PTPN5	1
NM_001039970	PTPN5	1
NM_006906	PTPN5	1
NM_207306		1
NM_152740	HIBADH	1
XM_937753	PRDM6	1
XM_927647	PRDM6	1
NM_020459		1
NM_020710	LRRC47	1
NM_015247	CYLD	1
NM_001042412	CYLD	1
NM_001042355	CYLD	1
NM_207445	FLJ39531	1
NM_003423	ZNF43	1
NM_032532	FNDC1	1
NM_004356	CD81	1
NM_005895	GOLGA3	1
NM_006288	THY1	1
NM_001044392	MUC1	1
NM_001044390	MUC1	1
NM_001044393	MUC1	1
NM_001044391	MUC1	1
NM_001018017	MUC1	1
NM_001018016	MUC1	1
NM_002456	MUC1	1
NM_002751	MAPK11	1
NM_144775	SMCR8	1
NM_005450	NOG	1
NM_194284	CLDN23	1
NM_007176	C14orf1	1
NM_006764	IFRD2	1
NM_182501	MTERFD2	1
NM_032444	BTBD12	1
NM_006336	ZER1	1
NM_001024457	RGPD1	1
NM_004831	CRSP7	1
NM_000092	COL4A4	1
NM_018313	PBRM1	1
NM_181042	PBRM1	1
NM_018165	PBRM1	1
NM_173569		1
NM_000333	ATXN7	1
NM_001127	AP1B1	1
NM_145730	AP1B1	1

---

Immunostimulatory motif search results

UGUGU - Judge et al. Sequence-dependent stimulation of the mammalian innate immune response by synthetic siRNA. Nat Biotechnol. 2005 Apr;23(4):457-62. (PMID: 15778705)
Motif found in sense strand

GUCCUUCAA - Hornung et al. Sequence-specific potent induction of IFN-alpha by short interfering RNA in plasmacytoid dendritic cells through TLR7. Nat Med. 2005 Mar;11(3):263-70. Epub 2005 Feb 20. (PMID: 15723075)
No Immunostimulatory motif found (GUCCUUCAA).

---

---

Settings used for search

Scores were calculated using scoring scheme SS1
Using AVG scoring mode.

Hit scores

Parameter	Spec. Score cut-off	Colour	Comment
Hit to same gene		Colour	Hits to the same accession or genes with 100 percent identity (i.e. likely transcript variants). It is advisable to always check the genes hit.
High scoring hit	≥80	Colour	Hits are very likely to cause an off-target effect
Medium scoring hit	<80¸≥40	Colour	Hits with a small chance of causing an off target effect.
Low scoring hit		Colour	Hits very unlikely to cause an off-target effect

The mismatches are colour coded based on the weighting of that position.

Cut-off	Colour
Match	Colour
Wobble base pairs (G-U)	Colour
Critical bases (none)	Colour
0.6 bits	Colour
0.4 bits	Colour
0.2 bits	Colour

About SpecificityServer

SpecificityServer is designed to help you identify potential non-specific matches to your siRNA. It incorporates the latest information about non-specific matches (sequence-specific only).

Designed by: Alistair Chalk, Quan Du, Zicai Liang and Erik L.L. Sonnhammer

For more details about the algorithm "siRNA specificity searching incorporating mismatch tolerance data" Bioinformatics (2008) 24 (10): 1316-1317